Dihydromyricetin supplementation during in vitro culture improves porcine oocyte developmental competence by regulating oxidative stress by Jing WANG, Qing-Guo JIN, Rong-Ping LIU, Xin-Qin WANG, Ying-Hua LI, Nam-Hyung KIM, Yong-Nan XU

“…Supplementation with 5 μM DHM during in vitro culture (IVC) significantly improved blastocyst formation rate and increased the total number of cells in porcine embryos. …”
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Oviduct Fluid Extracellular Vesicles Change the Phospholipid Composition of Bovine Embryos Developed In Vitro by Charles Banliat, Daniel Le Bourhis, Ophélie Bernardi, Daniel Tomas, Valérie Labas, Pascal Salvetti, Benoît Guyonnet, Pascal Mermillod, Marie Saint-Dizier

“…The development rates, cryotolerance, and total cell number of blastocysts were also evaluated. The exposure to oEVs did not affect blastocyst yield or cryotolerance but modified the phospholipid content of blastocysts with specific changes before and after blastocoel expansion. …”
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Effect of db-cAMP on embryonic development of bovine oocytes fertilized with sex sorted semen by Hugo Hernández Fonseca, Héctor Nava Trujillo, Saksiri Sirisathien, Lawrence Johnson, David Guthrie, Glenn Welch, Adirmo Hernández, Benjamin Brackett

“…Treatment with db-cAMP enabled a better (P menor 0.05) blastocyst formation rate (19.29%) compared to control (8.47%) and heparin (10.44%). …”
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Generation of Artificial Blastoids Combining miR-200-Mediated Reprogramming and Mechanical Cues by Georgia Pennarossa, Sharon Arcuri, Fulvio Gandolfi, Tiziana A. L. Brevini

“…In vitro-generated blastocyst-like structures are of great importance since they recapitulate specific features or processes of early embryogenesis, thus avoiding ethical concerns as well as increasing scalability and accessibility compared to the use of natural embryos. …”
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A theoretical model of dietary lipid variance as the origin of primary ciliary dysfunction in preeclampsia by Nancy R. Hart

“…During normal embryonic development, trophoblastic cells, which arise from the outer layer of the 4-day-old blastocyst, invade the maternal endometrium and establish extensive placental vascular connections between mother and fetus. …”
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Preimplantation development of embryos in women of advanced maternal age by O. V. Chaplia, J. V. Gontar, N. M. Bilko

“…Nevertheless, no substantial difference was found between blastocyst formation rate among embryos with similar genetic component regardless of the maternal age. …”
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Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos by Zhiguo Liu, Guangming Xiang, Kui Xu, Jingjing Che, Changjiang Xu, Kui Li, Bingyuan Wang, Yulian Mu

“…It was reported that the SCNT embryos that complete the first cell division sooner are more likely to develop to the blastocyst stage, suggesting their better developmental competence. …”
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Detecting Embryo Developmental Potential by Single Blastomere RNA-Seq by Monika Nõmm, Marilin Ivask, Pille Pärn, Ene Reimann, Sulev Kõks, Ülle Jaakma

“…This study was conducted to develop a single cell embryo biopsy technique and gene expression analysis method with a very low input volume to ensure normal embryo development and to see if there are differences in gene expression profiles between day-5 biopsied bovine embryos that developed into blastocysts and embryos arrested at morula stage. Out of the 65 biopsied morulae, 32 developed to blastocysts (49.2%). …”
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Conjoined Twins Complicating a Dichorionic Triplet Pregnancy after Intracytoplasmic Sperm Injection: A Case Report and Review of the Literature by Anna Eleftheriades, Panagiotis Christopoulos, Elsa Tsapakis, Ermioni Tsarna, Nikolaos F. Vlahos, Emmanouil Kalampokas, Daniele Bolla, Makarios Eleftheriades

“…This case report presents a rare case of conjoined twins complicating a dichorionic-diamniotic triplet pregnancy, achieved after intracytoplasmic sperm injection (ICSI) and blastocyst transfer. A 44-year-old woman was referred for chorionicity determination to our Fetal Medicine Centre due to suspicion of conjoined twins in a triplet pregnancy. …”
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Sperm Energy Restriction and Recovery (SER) Alters Epigenetic Marks during the First Cell Cycle of Development in Mice by Darya A. Tourzani, Qiangzong Yin, Erica A. Jackson, Oliver J. Rando, Pablo E. Visconti, Maria G. Gervasi

“…The sperm energy restriction and recovery (SER) treatment developed in our laboratory was shown to improve fertilization and blastocyst development following in vitro fertilization (IVF) in mice. …”
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Co‐culturing or conditioned medium of Sertoli cells: Which one supports in vitro maturation of bovine oocytes and developmental competency of resulting embryos? by Sina Nazifi, Hassan Nazari, Hossein Hassanpour, Ebrahim Ahmadi, Azita Afzali

“…Besides, the degenerated oocytes in the co‐culture group were significantly higher than those in the control, CM33 and CM39 groups (p < 0.05), and the lowest cleavage rate belonged to the co‐culture group (p < 0.05). The blastocyst rate was also lower in the co‐culture group than other groups and there was a significant difference between the control and two CM groups (p < 0.05). …”
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Establishing some Correlations between Certain Morphometric Parameters and Embryo Quality by Nicolae Păcală, Ioan Bencsik, Dorel Dronca, Gabi Dumitrescu, Ada Cean, Alexandra Ivan, Oana Ciobotaru

“…After statistical analyze of the data we found that the thickness of the zona pellucida can constitute a morphometric parameter that can be used as an indicator of subsequent development of the 2 cell embryos to morula and blastocyst stage respectively. The other morphometric parameters studied (outer and inner diameter, and outer and inner perimeter) cannot be used as indicators of the embryo development.…”
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Order from chaos: a case report of a healthy live birth from a genetically “chaotic” embryo by Joanna Lin, M.D., Wendy Vitek, M.D., Erin L. Scott, M.D., Ph.D.

“…A single embryo underwent trophectoderm (TE) biopsy at the blastocyst stage and was cryopreserved. PGT-A revealed a “chaotic” test result. …”
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Detection of condition-specific marker genes from RNA-seq data with MGFR by Khadija El Amrani, Gregorio Alanis-Lobato, Nancy Mah, Andreas Kurtz, Miguel A. Andrade-Navarro

“…Then, we performed a more detailed analysis for three of these datasets, which profile the transcriptomes of different human tissues, immune and human blastocyst cell types, respectively. MGFR’s predicted markers were compared to gold-standard lists for these datasets and outperformed the other marker detectors. …”
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Effect of method and media type for in vitro fertilization on equine (Equus ferus caballus) embryo development by Aryan Hussam, Mardenli Omar, Groza Ioan, Bogdan Liviu

“…Zygotes cultured in the CII medium achieved a higher rate of blastocyst formation (30%) compared to those in the CI medium (26.31%); p< 0.001. …”
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Embryo transfer of dorper breed to Mongolian sheep by Chuluunbayar Uuganbayar, Tsolmonbaatar Boldsaikhan, Byambasaikhan Danzan-Osor, Ho-Jun Lee, Sang-Hwan Kim, Enkhbolor Barsuren

“…After embryo recover, code 1 and 2 embryos (morula or blastocyst stage) for ET in the present study were 63% (63/100) and 24% (24/100), respectively. …”
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HEX expression and localization in normal mammary gland and breast carcinoma by Pandolfi Maura, Pestrin Marta, Manfioletti Guidalberto, Pellizzari Lucia, Puglisi Fabio, Puppin Cinzia, Piga Andrea, Di Loreto Carla, Damante Giuseppe

“…During early mouse development, HEX is expressed in the primitive endoderm of blastocyst. Later, HEX is expressed in developing thyroid, liver, lung, as well as in haematopoietic progenitors and endothelial cells. …”
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#186 : Ultra-Long Protocol During IVF Outcome in Infertile Women with Endometriosis by Dafi Yulinda, Louis Regan, Fahreza Akbar Siregar, Axel Jovito, Budi Wiweko

“…Parameters observed were maternal age, body mass index, oocytes retrieved, fertilization rate, blastocyst rate, and pregnancy rate. Result: There were 12 patients who underwent IVF and received ovarian stimulation with ultra long protocol. …”
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Sexually Dimorphic Gene Expression in X and Y Sperms Instructs Sexual Dimorphism of Embryonic Genome Activation in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) by Yang Xiong, Dan-Yang Wang, Wenjie Guo, Gaorui Gong, Zhen-Xia Chen, Qin Tang, Jie Mei

“…Through whole-genome re-sequencing (WGRS) of parental fish and RNA-seq of five early embryonic stages, we found the low-blastocyst time point is a key to maternal transcriptome degradation and zygotic genome activation. …”
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Rosmarinic acid treatment during porcine oocyte maturation attenuates oxidative stress and improves subsequent embryo development in vitro by Yan Zhang, Jing Guo, Xiao Wei Nie, Zi Yue Li, Yu Meng Wang, Shuang Liang, Suo Li

“…Results and Discussion The results showed that 5 µM RA treatment during the period of porcine oocyte IVM improves blastocyst quality and hatching ability after parthenogenetic activation. …”
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