Calcium ionophore improves embryonic development and pregnancy outcomes in patients with previous developmental problems in ICSI cycles by Xiaolei Chen, Haibin Zhao, Jiale Lv, Yi Dong, Maoning Zhao, Xinlei Sui, Ran Cui, Boyang Liu, Keliang Wu

“…Significantly higher day 3 good quality embryo rate (P = 0.034), good quality blastocyst formation rate (P <  0.001), and utilization rate (P <  0.001) were seen in AOA group. …”
Get full text

The duration of estrogen treatment before progesterone application does not affect neonatal and perinatal outcomes in frozen embryo transfer cycles by Junwei Zhang, Mingze Du, Zhongkai Wang, Sheling Wu, Yichun Guan, Lijun Sun

“…After multiple logistic regression analysis, the duration of estrogen treatment did not affect the rate of GDM or other outcomes.ConclusionThe estrogen treatment duration before progesterone application does not affect neonatal and perinatal outcomes in single frozen blastocyst transfer cycles.…”
Get full text

Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos by Lianguang Xu, Muhammad Idrees, Myeong-Don Joo, Tabinda Sidrat, Yiran Wei, Seok-Hwan Song, Kyeong-Lim Lee, Il-Keun Kong

“…<i>TERT</i> plasmid injection into pronuclear zygotes not only markedly enhanced day-8 blastocysts’ development competence (39.1 ± 0.8%) compared to the control (31.1 ± 0.5%) and D-galactose (17.9 ± 1.0%) treatment groups but also enhanced <i>KLB</i> and <i>FGFR1</i> expression. …”
Get full text

Case report: Preimplantation genetic testing for infantile GM1 gangliosidosis by Valeria A. Zagaynova, Yulia A. Nasykhova, Ziravard N. Tonyan, Maria M. Danilova, Natalya M. Dvoynova, Tatyana E. Lazareva, Tatyana E. Ivashchenko, Elena S. Shabanova, Inna O. Krikheli, Elena A. Lesik, Olesya N. Bespalova, Igor Yu. Kogan, Andrey S. Glotov

“…Trophectoderm biopsy was performed on 8 out of 10 resulting embryos at the blastocyst stage. To perform PGT-M, we developed a novel testing system, allowing for direct analysis of disease-causing mutations, as well as haplotype analysis based on the study of polymorphic markers—short tandem repeats (STR), located upstream and downstream of the GLB1 gene. …”
Get full text

Melatonin Abrogates the Anti-Developmental Effect of the AKT Inhibitor SH6 in Bovine Oocytes and Embryos by Marwa El Sheikh, Ayman Mesalam, Ahmed Atef Mesalam, Muhammad Idrees, Kyeong-Lim Lee, Il-Keun Kong

“…Results showed that the inclusion of SH6, a specific AKT inhibitor, during in vitro maturation (IVM) significantly reduced oocyte maturation, cumulus cell expansion, cleavage, and blastocyst development that were rescued upon addition of melatonin. …”
Get full text

Modified <i>Spirulina maxima</i> Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes by Pantu-Kumar Roy, Ahmad-Yar Qamar, Bereket-Molla Tanga, Seonggyu Bang, Gyeonghwan Seong, Xun Fang, Ghangyong Kim, Shan-Lakmal Edirisinghe, Mahanama De Zoysa, Do-Hyung Kang, Islam M. Saadeldin, Jongki Cho

“…Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. …”
Get full text

Cumulative live birth rates after IVF/ICSI cycles with sperm prepared by density gradient centrifugation vs. swim-up: a retrospective study using a propensity score-matching analys... by Meng Rao, Li Tang, Longda Wang, Mengxiang Chen, Gaofeng Yan, Shuhua Zhao

“…After adjusting for confounders, the recovery rate, progressive motility rate after sperm preparation, fertilization rate, good-quality embryo rate, and blastocyst formation rate were similar between the DGC and SU groups. …”
Get full text

cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes by Daniela-Alejandra Medina-Chávez, Irene Sánchez-Ajofrín, Patricia Peris-Frau, Carolina Maside, Vidal Montoro, Rocío Fernández-Santos, José Julián Garde, Ana Josefa Soler

“…Results showed that pre-IVM treatment significantly decreased (<i>p</i> < 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% ± 3.51% vs. control = 20.58% ± 3.51%) and increased (<i>p</i> ≤ 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% ± 1.47% vs. control = 18.22% ± 1.47%, and from the cleaved embryos: pre-IVM = 45.16% ± 1.73% vs. control = 32.88% ± 1.73%). …”
Get full text

Improvement of post-thaw quality and fertilizing ability of bull spermatozoa using Rho kinase inhibitor in freezing extender by Mina Behnam, Reza Asadpour, Tohid Rezaei Topraggaleh, Tohid Rezaei Topraggaleh, Hossein Hamali

“…The results of IVF demonstrated that the treatment of frozen–thawed spermatozoa with 20 μM Y-27632 increased blastocyst rates compared to the control group (p &lt; 0.05). …”
Get full text

Characterization of Glutathione Peroxidase 4 in Rat Oocytes, Preimplantation Embryos, and Selected Maternal Tissues during Early Development and Implantation by Andrea Kreheľová, Veronika Kovaříková, Iveta Domoráková, Peter Solár, Alena Pastornická, Andriana Pavliuk-Karachevtseva, Silvia Rybárová, Ingrid Hodorová, Jozef Mihalik

“…Whereas in the oocytes, GPx4 was homogeneously diffused, in the blastomeres, granules were formed, and in the blastocysts, even clusters were present mainly around the cell nuclei. …”
Get full text

Derivation of Arbas Cashmere Goat Induced Pluripotent Stem Cells in LCDM with Trophectoderm Lineage Differentiation and Interspecies Chimeric Abilities by Fang Liu, Jing Wang, Yongli Yue, Chen Li, Xuemin Zhang, Jinzhu Xiang, Hanning Wang, Xueling Li

“…The giPSCs contributed to embryonic and extraembryonic tissue in preimplantation blastocysts and postimplantation chimeric embryos. RNA-sequencing analysis showed that the giPSCs were very close to goat embryos at the blastocyst stage and giPSCs have similar properties to typical extended PSCs (EPSCs). …”
Get full text

Original delayed-start ovarian stimulation protocol with a gonadotropin-releasing hormone antagonist, medroxyprogesterone acetate, and high-dose gonadotropin for poor responders an... by Kazuhiro Takeuchi, Yuji Orita, Tokiko Iwakawa, Yukari Kuwatsuru, Yuko Kuroki, Yumiko Fukumoto, Yamato Mizobe, Mari Tokudome, Harue Moewaki

“…Similar results were obtained in Group B (n=83; 2PN zygotes [1.7 ± 1.7 versus 2.3 ± 1.8], good blastocysts [0.4 ± 0.7 versus 0.9 ± 1.3], live birth rates [0 versus 18.1]) and Group C (n=73; MII oocytes [5.1 ± 3.8 versus 6.6 ± 4.0], 2PN zygotes [4.0 ± 3.4 versus 5.4 ± 3.4], good blastocysts [0.7 ± 1.1 versus 1.6 ± 1.9], and live birth rates [1.4 versus 31.5]).ConclusionThis original protocol increased the number of MII oocytes retrieved, 2PN zygotes, good blastocysts, and live birth rates in both poor responders and in patients with poor-quality embryos.…”
Get full text

Controlled ovarian hyperstimulation parameters are not associated with de novo chromosomal abnormality rates and clinical pregnancy outcomes in preimplantation genetic testing by Yanli Liu, Junhan Shen, Yuchao Zhang, Rui Peng, Junliang Zhao, Pengfei Zhou, Rujing Yang, Yichun Guan

“…ResultsA total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. …”
Get full text

#92 : An Artificial Intelligence Algorithm Outperforms Highly Variable Embryologist Grading for Predicting the Likelihood of Pregnancy Outcome from Embryo Images by Matthew VerMilyea, Jonathan Hall, Michelle Perugini, Tuc Nguyen, Don Perugini, Sonya Diakiw

“…Method: 20 images of blastocyst-stage embryos on day 5 of in vitro development were selected to represent a range of morphological qualities. …”
Get full text

Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates by Andrea Fernández-Montoro, Daniel Angel-Velez, Camilla Benedetti, Nima Azari-Dolatabad, Osvaldo Bogado Pascottini, Ann Van Soom, Krishna Chaitanya Pavani

“…However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. …”
Get full text

Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant by Hansol JIN, Wooyoon CHOI, Kazuaki MATSUMURA, Suong-hyu HYON, Yuki GEN, Masakazu HAYASHI, Tadahiro KAWABATA, Moe IJIRI, Kazuchika MIYOSHI

“…The concentration of CPLL in the freezing extender did not affect the ability of frozen-thawed spermatozoa to fertilize oocytes in vitro. However, the blastocyst formation rate of embryos derived from spermatozoa cryopreserved with 0.25% CPLL (24.6%) was significantly higher (P &lt; 0.01) than that of embryos derived from spermatozoa cryopreserved without CPLL (11.2%). …”
Get full text

Pre-Implantation Genetic Testing for Oculocutaneous Albinism Type 1 Using Karyomapping by Sirivipa Piyamongkol, Suchada Mongkolchaipak, Winai Chaidaroon, Tawiwan Pantasri, Rungthiwa Sirapat, Wanwisa Suriya, Theera Tongsong, Wirawit Piyamongkol

“…In the clinical PGT cycle, two blastocyst stage embryos were subjected to PGT. Karyotyping PGT results of OCA1 revealed both of the embryos to be normal. …”
Get full text

Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology by Lianguang Xu, Seok-Hwan Song, Muhammad Idrees, Ayman Mesalam, Myeong-Don Joo, Tabinda Sidrat, Yiran Wei, Kyeong-Lim Lee, Wenfa Lu, Il-Keun Kong

“…We initially used AF cells to develop cloned embryos and then cultured the cloned day-8 blastocysts for 10 days to obtain ECs as donor cells for second embryo cloning. …”
Get full text

Effect of different levels of serum progesterone on day of frozen ET on clinical outcome by Sedigheh Hosseini, Saghar Salehpour, Parisa Taherzadeh Borujeni, Nazanin Hajizadeh

“…Conclusion The current study demonstrated that high and low serum progesterone (P4) levels on embryo transfer day were associated with reduced clinical outcomes following blastocyst transfer during IVF/ICSI. However, more studies with large sizes are required in this regard.…”
Get full text

Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos by Weihua XU, Hongyi LI, Mao ZHANG, Junsong SHI, Zhengchao WANG

“…Further research should focus on mechanisms underlying demethylation during the early cleavage of embryos and de novo DNA methylation at the blastocyst stage.…”
Get full text