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1261
Calcium ionophore improves embryonic development and pregnancy outcomes in patients with previous developmental problems in ICSI cycles
Published 2022-12-01“…Significantly higher day 3 good quality embryo rate (P = 0.034), good quality blastocyst formation rate (P < 0.001), and utilization rate (P < 0.001) were seen in AOA group. …”
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1262
The duration of estrogen treatment before progesterone application does not affect neonatal and perinatal outcomes in frozen embryo transfer cycles
Published 2023-07-01“…After multiple logistic regression analysis, the duration of estrogen treatment did not affect the rate of GDM or other outcomes.ConclusionThe estrogen treatment duration before progesterone application does not affect neonatal and perinatal outcomes in single frozen blastocyst transfer cycles.…”
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1263
Constitutive Expression of TERT Enhances β-Klotho Expression and Improves Age-Related Deterioration in Early Bovine Embryos
Published 2021-05-01“…<i>TERT</i> plasmid injection into pronuclear zygotes not only markedly enhanced day-8 blastocysts’ development competence (39.1 ± 0.8%) compared to the control (31.1 ± 0.5%) and D-galactose (17.9 ± 1.0%) treatment groups but also enhanced <i>KLB</i> and <i>FGFR1</i> expression. …”
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1264
Case report: Preimplantation genetic testing for infantile GM1 gangliosidosis
Published 2024-02-01“…Trophectoderm biopsy was performed on 8 out of 10 resulting embryos at the blastocyst stage. To perform PGT-M, we developed a novel testing system, allowing for direct analysis of disease-causing mutations, as well as haplotype analysis based on the study of polymorphic markers—short tandem repeats (STR), located upstream and downstream of the GLB1 gene. …”
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1265
Melatonin Abrogates the Anti-Developmental Effect of the AKT Inhibitor SH6 in Bovine Oocytes and Embryos
Published 2019-06-01“…Results showed that the inclusion of SH6, a specific AKT inhibitor, during in vitro maturation (IVM) significantly reduced oocyte maturation, cumulus cell expansion, cleavage, and blastocyst development that were rescued upon addition of melatonin. …”
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1266
Modified <i>Spirulina maxima</i> Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes
Published 2021-08-01“…Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. …”
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1267
Cumulative live birth rates after IVF/ICSI cycles with sperm prepared by density gradient centrifugation vs. swim-up: a retrospective study using a propensity score-matching analys...
Published 2022-03-01“…After adjusting for confounders, the recovery rate, progressive motility rate after sperm preparation, fertilization rate, good-quality embryo rate, and blastocyst formation rate were similar between the DGC and SU groups. …”
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1268
cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
Published 2021-08-01“…Results showed that pre-IVM treatment significantly decreased (<i>p</i> < 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% ± 3.51% vs. control = 20.58% ± 3.51%) and increased (<i>p</i> ≤ 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% ± 1.47% vs. control = 18.22% ± 1.47%, and from the cleaved embryos: pre-IVM = 45.16% ± 1.73% vs. control = 32.88% ± 1.73%). …”
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1269
Improvement of post-thaw quality and fertilizing ability of bull spermatozoa using Rho kinase inhibitor in freezing extender
Published 2023-07-01“…The results of IVF demonstrated that the treatment of frozen–thawed spermatozoa with 20 μM Y-27632 increased blastocyst rates compared to the control group (p < 0.05). …”
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1270
Characterization of Glutathione Peroxidase 4 in Rat Oocytes, Preimplantation Embryos, and Selected Maternal Tissues during Early Development and Implantation
Published 2021-05-01“…Whereas in the oocytes, GPx4 was homogeneously diffused, in the blastomeres, granules were formed, and in the blastocysts, even clusters were present mainly around the cell nuclei. …”
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1271
Derivation of Arbas Cashmere Goat Induced Pluripotent Stem Cells in LCDM with Trophectoderm Lineage Differentiation and Interspecies Chimeric Abilities
Published 2023-09-01“…The giPSCs contributed to embryonic and extraembryonic tissue in preimplantation blastocysts and postimplantation chimeric embryos. RNA-sequencing analysis showed that the giPSCs were very close to goat embryos at the blastocyst stage and giPSCs have similar properties to typical extended PSCs (EPSCs). …”
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1272
Original delayed-start ovarian stimulation protocol with a gonadotropin-releasing hormone antagonist, medroxyprogesterone acetate, and high-dose gonadotropin for poor responders an...
Published 2023-10-01“…Similar results were obtained in Group B (n=83; 2PN zygotes [1.7 ± 1.7 versus 2.3 ± 1.8], good blastocysts [0.4 ± 0.7 versus 0.9 ± 1.3], live birth rates [0 versus 18.1]) and Group C (n=73; MII oocytes [5.1 ± 3.8 versus 6.6 ± 4.0], 2PN zygotes [4.0 ± 3.4 versus 5.4 ± 3.4], good blastocysts [0.7 ± 1.1 versus 1.6 ± 1.9], and live birth rates [1.4 versus 31.5]).ConclusionThis original protocol increased the number of MII oocytes retrieved, 2PN zygotes, good blastocysts, and live birth rates in both poor responders and in patients with poor-quality embryos.…”
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1273
Controlled ovarian hyperstimulation parameters are not associated with de novo chromosomal abnormality rates and clinical pregnancy outcomes in preimplantation genetic testing
Published 2023-01-01“…ResultsA total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. …”
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1274
#92 : An Artificial Intelligence Algorithm Outperforms Highly Variable Embryologist Grading for Predicting the Likelihood of Pregnancy Outcome from Embryo Images
Published 2023-12-01“…Method: 20 images of blastocyst-stage embryos on day 5 of in vitro development were selected to represent a range of morphological qualities. …”
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1275
Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates
Published 2023-05-01“…However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. …”
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1276
Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant
Published 2022-07-01“…The concentration of CPLL in the freezing extender did not affect the ability of frozen-thawed spermatozoa to fertilize oocytes in vitro. However, the blastocyst formation rate of embryos derived from spermatozoa cryopreserved with 0.25% CPLL (24.6%) was significantly higher (P < 0.01) than that of embryos derived from spermatozoa cryopreserved without CPLL (11.2%). …”
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1277
Pre-Implantation Genetic Testing for Oculocutaneous Albinism Type 1 Using Karyomapping
Published 2022-05-01“…In the clinical PGT cycle, two blastocyst stage embryos were subjected to PGT. Karyotyping PGT results of OCA1 revealed both of the embryos to be normal. …”
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1278
Effects of Donor Cell Types on the Development of Bovine Embryos Using Cytoplasm Injection Cloning Technology
Published 2021-05-01“…We initially used AF cells to develop cloned embryos and then cultured the cloned day-8 blastocysts for 10 days to obtain ECs as donor cells for second embryo cloning. …”
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1279
Effect of different levels of serum progesterone on day of frozen ET on clinical outcome
Published 2023-10-01“…Conclusion The current study demonstrated that high and low serum progesterone (P4) levels on embryo transfer day were associated with reduced clinical outcomes following blastocyst transfer during IVF/ICSI. However, more studies with large sizes are required in this regard.…”
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1280
Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos
Published 2020-09-01“…Further research should focus on mechanisms underlying demethylation during the early cleavage of embryos and de novo DNA methylation at the blastocyst stage.…”
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