Elevation of MPF and MAPK gene expression, GSH content and mitochondrial distribution quality induced by melatonin promotes porcine oocyte maturation and development in vitro by Zimo Zhao, Ling Yang, Dan Zhang, Zi Zheng, Ning Li, Qianjun Li, Maosheng Cui

“…As far as the embryonic developmental potential is concerned, the cleavage and blastocyst rate of oocytes cultured with 10−6 and 10−9 mol/L melatonin was significantly higher than the 10−12 mol/L melatonin and control. …”
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The histone lysine demethylase KDM7A is required for normal development and first cell lineage specification in porcine embryos by Vitor Braga Rissi, Werner Giehl Glanzner, Mariana Priotto De Macedo, Karina Gutierrez, Hernan Baldassarre, Paulo Bayard Dias Gonçalves, Vilceu Bordignon

“…However, the ratio of inner cell mass (ICM)/total number of cells in D7 blastocysts was reduced by 45.5% in KDM7A knockdown compared to control embryos. …”
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The embryo mosaicism profile of next-generation sequencing PGT-A in different clinical conditions and their associations by Hadassa Campos Heiser, Natalia Fagundes Cagnin, Mariane Uehara de Souza, Taccyanna Mikulski Ali, Paula Regina Queiroz Estrada, Camila Cristina Wuaquim Dantas de Souza, Bruno Coprerski, Carmen Rubio, Marcia Riboldi

“…We identified associations between low (negatively-associated) and high mosaicism (positively-associated) and maternal age, indication (male factor and uterus/ovarian factor negatively associated with low and high mosaic, respectively), day of blastocyst development (day five has an overall better outcome), morphology grade (lower quality increased the chances of low and high mosaicism), origin (vitrified oocyte and embryo increased the rates of low and high mosaicism, respectively), and embryo sex (male embryos negatively associated with low mosaic).DiscussionWith these results, we hope to foster an improved understanding of the chromosomal mosaicism linked with distinct clinical conditions and their associations in Brazil.…”
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Validation of Non-Invasive Preimplantation Genetic Screening Using a Routine IVF Laboratory Workflow by Ni-Chin Tsai, Yun-Chiao Chang, Yi-Ru Su, Yi-Chi Lin, Pei-Ling Weng, Yin-Hua Cheng, Yi-Ling Li, Kuo-Chung Lan

“…All embryos were cultured to the blastocyst stage (day 5 or 6) in a single-embryo culture time-lapse incubator. …”
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Stem cell factor’s role in enhancing the quality of fertilized and cloned porcine embryos for improved embryonic stem cell derivation by Lian Cai, Lian Cai, Lian Cai, Sang-Hwan Hyun, Sang-Hwan Hyun, Sang-Hwan Hyun, Eunhye Kim

“…The rates of cleavage and blastocyst formation exhibited no significant differences between fertilized and cloned embryos, even with the addition of SCF. …”
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Essential role of Mg2+ in mouse preimplantation embryo development revealed by TRPM7 chanzyme-deficient gametes by Neha Gupta, Cristina Soriano-Úbeda, Paula Stein, Virginia Savy, Brian N. Papas, Goli Ardestani, Ingrid Carvacho, Dominique Alfandari, Carmen J. Williams, Rafael A. Fissore

“…Using gamete-specific Trpm7-null lines, we report that TRPM7-mediated Mg2+ influx is indispensable for reaching the blastocyst stage. TRPM7 is expressed dynamically from gametes to blastocysts; displays stage-specific localization on the plasma membrane, cytoplasm, and nucleus; and undergoes cleavage that produces C-terminal kinase fragments. …”
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Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development by Tabinda Sidrat, Abdul Aziz Khan, Myeon-Don Joo, Yiran Wei, Kyeong-Lim Lee, Lianguang Xu, Il-Keun Kong

“…Oviduct flushing is enriched by a wide variety of nutrients that guide the 3–4 days journey of pre-implantation embryo through the oviduct as it develops into a competent blastocyst (BL). However, little is known about the specific requirement and role of these nutrients that orchestrate the early stages of embryonic development. …”
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Hypoxia-sensitive miRNA regulation via CRISPR/dCas9 loaded in hybrid exosomes: A novel strategy to improve embryo implantation and prevent placental insufficiency during pregnancy by Alireza Yaghoobi, Yasaman Nazerian, Arman Zeinaddini Meymand, Ali Ansari, Amirhossein Nazerian, Hassan Niknejad

“…In order to avoid concerns about the fetus’s genetic manipulation, our vector would be transfected specifically into the trophoblast layer of the blastocyst via binding to trophoblast Erb-B4 receptors without entering the inner cell mass. …”
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Nicotinamide Supplementation during the In Vitro Maturation of Oocytes Improves the Developmental Competence of Preimplantation Embryos: Potential Link to SIRT1/AKT Signaling by Marwa El Sheikh, Ahmed Atef Mesalam, Muhammad Idrees, Tabinda Sidrat, Ayman Mesalam, Kyeong-Lim Lee, Il-Keun Kong

“…Remarkably, the hatching rates of day-7 and day-8 blastocysts were significantly improved under 0.1 mM NAM treatment. …”
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The Effects of Heat Shock Protein 70 Addition in the Culture Medium on the Development and Quality of In Vitro Produced Heat Shocked Bovine Embryos by Konstantina Stamperna, Themistoklis Giannoulis, Eleni Dovolou, Maria Kalemkeridou, Ioannis Nanas, Katerina Dadouli, Katerina Moutou, Zissis Mamuris, Georgios S. Amiridis

“…Cleavage and embryo yield were assessed 48 h p.i. and on days 7, 8, 9, respectively and gene expression in day 7 blastocysts was assessed by RT-PCR. Blastocyst yield was the highest in group C39; and higher in group H41 compared to group C41. …”
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The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey by Kui Duan, Chen-Yang Si, Shu-Mei Zhao, Zong-Yong Ai, Bao-Hua Niu, Yu Yin, Li-Feng Xiang, Hao Ding, Yun Zheng

“…Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. …”
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Assessment of artificial intelligence model and manual morphokinetic annotation system as embryo grading methods for successful live birth prediction: a retrospective monocentric s... by Myrto-Sotiria Papamentzelopoulou, Ilectra-Niki Prifti, Despoina Mavrogianni, Thomais Tseva, Ntilay Soyhan, Aikaterini Athanasiou, Antonia Athanasiou, Adamantios Athanasiou, Paraskevi Vogiatzi, George Konomos, Dimitrios Loutradis, Maria Sakellariou

“…Therefore, the present study aims at investigating the efficiency of KIDScore and iDAScore systems for blastocyst stage embryos in predicting live birth events. …”
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The modulator role of Urtica dioica on deleterious effects of retinoic acid high doses on histological parameters and fertilization of rats by Shuli Du, Lijuan Wang, Yinghui Wang, Yanna Jin, Aijing Wang, Cuiting Lv, Razzagh Abedi-Firouzjah

“…The rate of oocyte maturation, 2-cell–4-cell and 4-cell–8-cell embryos, and blastocyst formation increased significantly in the groups in which UD extracts were administered compared to the control and RA groups. …”
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Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stress on bovine oocytes and embryos by Nico G. Menjivar, Ahmed Gad, Ahmed Gad, Samuel Gebremedhn, Samuel Gebremedhn, Soham Ghosh, Dawit Tesfaye

“…Moreover, our findings indicate a carryover impact from the addition of GC-EVs during oocyte maturation in the development to the blastocyst stage with enhanced viability.…”
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Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence by Cecilia Valencia, Felipe Pérez-García, Luis Aguila, Ricardo Felmer, María Elena Arias

“…The quality of blastocysts in terms of cell counts, their allocation, and the numbers of TUNEL-positive cells did not differ among groups. …”
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Glutathione Ethyl Ester Protects In Vitro<i>-</i>Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming by Tania García-Martínez, Meritxell Vendrell-Flotats, Iris Martínez-Rodero, Erika Alina Ordóñez-León, Manuel Álvarez-Rodríguez, Manel López-Béjar, Marc Yeste, Teresa Mogas

“…No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of <i>GPX1</i>, <i>AQP3</i> and <i>CX43</i> in derived blastocysts. …”
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Anti-Müllerian Hormone and OPU-ICSI Outcome in the Mare by Marion Papas, Jan Govaere, Sofie Peere, Ilse Gerits, Margot Van de Velde, Daniel Angel-Velez, Tine De Coster, Ann Van Soom, Katrien Smits

“…Mares with a high AMH level (≥2.5 µg/L) yielded significantly greater numbers of follicles (22.9 ± 1.2), oocytes (13.5 ± 0.8), and blastocysts (2.1 ± 0.4) per OPU-ICSI session compared to mares with medium (1.5–2.5 µg/L) or low AMH levels (<1.5 µg/L), but no significant differences in blastocyst rates were observed. …”
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Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes by Yu Wang, Jia-Jia Qi, Yi-Jing Yin, Hao Jiang, Jia-Bao Zhang, Shuang Liang, Bao Yuan

“…Compared to the control group, supplementation with 5 μM FA significantly enhanced the maturation rates of bovine oocytes and the expansion of the cumulus cells area, as well as the subsequent cleavage and blastocyst formation rates after in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). …”
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Profiling the transcriptomic signatures and identifying the patterns of zygotic genome activation – a comparative analysis between early porcine embryos and their counterparts in o... by Yanhui Zhai, Hao Yu, Xinglan An, Zhiren Zhang, Meng Zhang, Sheng Zhang, Qi Li, Ziyi Li

“…By treating with 10,058-F4, an inhibitor of c-MYC, the cleavage rate (38.33 ± 3.4%) and blastocyst rate (23.33 ± 4.3%) of porcine embryos were significantly lower than those of the control group (50.82 ± 2.7% and 34.43 ± 1.9%). …”
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Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation by Sanghoon Lee, Hyo-Gu Kang, Pil-Soo Jeong, Tsevelmaa Nanjidsuren, Bong-Seok Song, Yeung Bae Jin, Sang-Rae Lee, Sun-Uk Kim, Bo-Woong Sim

“…After in vitro fertilization, the BCB+ group showed a significantly higher monospermy rate, fertilization efficiency, percentage of cleavage and blastocyst formation, with a higher total cell number and a lower apoptosis in blastocysts as compared with the BCB- group. …”
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