Cattle phenotypes can disguise their maternal ancestry by Kanokwan Srirattana, Kieren McCosker, Tim Schatz, Justin C. St. John

“…However, embryos derived from oocytes with Bos indicus mtDNA had the same ability to develop to the blastocyst stage and the levels of mtDNA copy number in their blastocysts were similar to blastocysts derived from oocytes harbouring Bos taurus mtDNA. …”
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Extracellular Vesicles from Follicular and Ampullary Fluid Isolated by Density Gradient Ultracentrifugation Improve Bovine Embryo Development and Quality by Anise Asaadi, Nima Azari Dolatabad, Hadi Atashi, Annelies Raes, Petra Van Damme, Michael Hoelker, An Hendrix, Osvaldo Bogado Pascottini, Ann Van Soom, Mojtaba Kafi, Krishna Chaitanya Pavani

“…The characterization of EVs isolated using ODG UC or SEC from FF and AOF did not show any differences in terms of EV sizes (40–400 nm) and concentrations (2.4 ± 0.2 × 10¹²−1.8 ± 0.2 × 10¹³ particles/mL). Blastocyst yield and quality was higher in groups supplemented with EVs isolated from FF and AOF by ODG UC, with higher total cell numbers and a lower apoptotic cell ratio compared with the other groups (<i>p</i> < 0.05). …”
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ART outcomes during the COVID-19 pandemic by Isaac J. Chamani, James Grifo, Monica S. Chung, Laurie J. McKenzie, David H. McCulloh, William E. Gibbons, Frederick L. Licciarid

“…Results: We found no consistent significant differences in the number of oocytes retrieved, embryos fertilized, blastocysts or euploid embryos produced at either institution over the study period. …”
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Effect of use and dosage of p-follicle-stimulating hormone for ovarian superstimulation before ovum pick-up and in vitro embryo production in pregnant Holstein heifers by Cameron B. Hayden, Rodrigo V. Sala, Daniela C. Pereira, Juan F. Moreno, Alvaro García-Guerra

“…Cleavage percentage, number of cleaved oocytes, blastocyst percentage, and number of blastocysts increased linearly with increasing p-FSH dose. …”
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High-Throughput In Vitro Screening Identified Nemadipine as a Novel Suppressor of Embryo Implantation by Xian Chen, Sudini Ranshaya Fernando, Yin-Lau Lee, William Shu-Biu Yeung, Ernest Hung-Yu Ng, Raymond Hang-Wun Li, Kai-Fai Lee

“…To explore the use of non-hormonal compounds that suppress embryo implantation, we established a high-throughput spheroid-endometrial epithelial cell co-culture assay to screen the Library of Pharmacologically Active Compounds (LOPAC) for compounds that affect trophoblastic spheroid (blastocyst surrogate) attachment onto endometrial epithelial Ishikawa cells. …”
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Iron overload in endometriosis peritoneal fluid induces early embryo ferroptosis mediated by HMOX1 by Shishi Li, Yier Zhou, Qiongxiao Huang, Xiaohua Fu, Ling Zhang, Fang Gao, Zhen Jin, Limei Wu, Chongyi Shu, Xirong Zhang, Weihai Xu, Jing Shu

“…Iron overload in endometriosis PF disrupted blastocyst formation, decreased GPX4 expression and induced lipid peroxidation, suggesting that iron overload causes embryotoxicity and induces ferroptosis. …”
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Molecular determinants of epithelial mesenchymal transition in mouse placenta and trophoblast stem cell by Shipra Kanti Jena, Shreya Das, Shreeta Chakraborty, Rupasri Ain

“…Abstract Trophectoderm cells of the blastocyst are the precursor of the placenta that is comprised of trophoblast, endothelial and smooth muscle cells. …”
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Cluster analysis reveals a homogeneous subgroup of PCOS women with metabolic disturbance associated with adverse reproductive outcomes by Hanxiao Chen, Rujun Zeng, Xun Zeng, Lang Qin, Yanjie Yin

“…Compared with PCOS patients in reproductive and balanced clusters, those in metabolic cluster had lower rates of good quality day 3 embryo and blastocyst formation. Moreover, PCOS patients in the reproductive cluster had greater fresh embryo transfer (ET) cancelation rate and clinical pregnancy rate after fresh ET than metabolic cluster (odds ratio [OR] = 3.37, 95% confidence interval [CI]: 1.77–6.44, and OR = 6.19, 95% CI: 1.58–24.24, respectively). …”
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Superfetation and heterotopic pregnancy: Case report of two rare phenomena coexisting and implications in the era of assisted reproductive technologies by Merhan Badran, Mazed Labib, Omar Abouali, Prakriti Pokhrel

“…Various theories have been proposed to explain its etiology, including polyovulation, delayed blastocyst implantation, and abnormal estrogen and b‐hCG surges. …”
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The Sperm DNA Fragmentation Assay with SDF Level Less Than 15% Provides a Useful Prediction for Clinical Pregnancy and Live Birth for Women Aged under 40 Years by Shiao Chuan Chua, Steven John Yovich, Peter Michael Hinchliffe, John Lui Yovich

“…Overall, there was no difference in the rates of blastocyst development nor clinical pregnancy rates between the two SDF groups, but there was a significantly higher pregnancy rate for the younger women (<35 years) with the group of SDF level < 15% (44.1% vs. 37.4%; <i>p</i> = 0.04). …”
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#104 : Live Birth Rate After Preimplantation Genetic Testing for Monogenic Disorders (PGT-M) for Thalassemia in Vietnamese Patients by Lan Tram Ho, Tam Luu, Cam Tran, Nhung Nguyen, Anh Le, Tri Nguyen, Mai Ma, Vu Ho, Anh Ha, Tuong Ho

“…The rate of unaffected blastocysts was 38.0% and heterozygous blastocysts was 27.2% among euploid blastocysts. …”
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A combination containing natural extracts of clove, Sophora flower bud, and yam improves fertility in aged female mice via multiple mechanisms by Wei Liu, Xinyu Wang, Yating Chen, Huiyu Zhang, Jing Chen, Jing Zhang, Tinghe Wu, Tinghe Wu, Jing Li

“…We also observed increased blastocyst formation when mature oocytes from control and DACHAO treated mice were for IVF and in vitro embryo culture. …”
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Activation of Blood Vessel Development in Endometrial Stromal Cells In Vitro Cocultured with Human Peri-Implantation Embryos Revealed by Single-Cell RNA-Seq by Bo Lv, Xiaoyu Xu, Xunyi Zhang, Lingbin Qi, Wen He, Lu Wang, Xian Chen, Luying Peng, Jinfeng Xue, Yazhong Ji, Zhigang Xue

“…In humans, the maternal endometrium participates in the physical and physiological interaction with the blastocyst to begin implantation. A bidirectional crosstalk is critical for normal implantation and then a successful pregnancy. …”
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Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation by Shiro YAMASHITA, Yuhei KOGASAKA, Yuuki HIRADATE, Kentaro TANEMURA, Yutaka SENDAI

“…We also found bi-allelic, as well as mono-allelic, non-mosaic homozygous mutations in the blastocysts. Most importantly, co-delivery of mTrex2 mRNA with CRISPR/Cas9 increased non-mosaic mutant blastocysts (29.3 ± 4.5%) and reduced mosaic mutant blastocysts (70.7 ± 4.5%) as compared to CRISPR/Cas9 alone (5.6 ± 6.4% and 92.6 ± 8.6%, respectively). …”
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SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION by A. V. Lapshtaeva, I. V. Evsegneeva, V. V. Novikov, I. V. Sychev, A. V. Karaulov

“…Moreover, IL-1α may participate in formation of pro-inflammatory environment for successful blastocyst implantation.…”
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Human TIMP1 Is a Growth Factor That Improves Oocyte Developmental Competence by Yolanda Manríquez-Treviño, Blanca Sánchez-Ramírez, Juan Alberto Grado-Ahuir, Beatriz Castro-Valenzuela, Carmen González-Horta, M.Eduviges Burrola-Barraza

“…In a parthenogenesis assay, oocyte IVM in 50 ng/mL of TIMP1 produced the most blastocyst compared to the other treatments (<i>p</i> < 0.05). …”
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Human embryonic stem cells with biological and epigenetic to those of mouse ESCs by Jaenisch, Rudolf, Hanna, Jacob, Cheng, Albert W., Saha, Krishanu, Kim, Jongpil, Lengner, Christopher J., Soldner, Frank, Cassady, John P., Muffat, Julien, Carey, Bryce W.

“…Human and mouse embryonic stem cells (ESCs) are derived from blastocyst-stage embryos but have very different biological properties, and molecular analyses suggest that the pluripotent state of human ESCs isolated so far corresponds to that of mouse-derived epiblast stem cells (EpiSCs). …”
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Cytogenetic and epigenetic analysis of human gametes and preimplantation embryos by Babariya, D

“…A multiplex PCR based bisulfite sequencing approach was successfully developed which was further utilised to study imprinting patterns of 23 imprinted genes simultaneously in trophectoderm and inner cell mass biopsies from euploid human blastocysts. To our knowledge, this is the first study to successfully analyse the methylation patterns of a large number of imprinted genes simultaneously in human blastocysts.…”

Relationships between follicle, oocyte and embryo features and the developmental potential of human IVF embryos : an investigation by Saith, R

“…The patterns of features characterising the 'good' embryos that resulted in triplet pregnancies on 3 embryo transfer or high hCG secretion <em>in vitro</em> and the 'bad' embryos that did not progress to the blastocyst stage on <em>in vitro</em> culture were identified. …”

Improved in vitro development of cloned bovine embryos using S-Adenosylhomocysteine, a non-toxic epigenetic modifying reagent. by Jafari, Shahram, Hosseini, Morteza S., Hajian, Mahdi, Forouzanfar, Mohsen, Jafarpour, Farnoosh, Abedi, Parvaneh, Ostadhosseini, Somayyeh, Abbasi, Hassan, Gourabi, Hamid, Shahverdi, Abdol H., Taghi Dizaj, Ahmad Vosough, Anjomshoaa, Maryam, Haron, Abd Wahid, Nordin, Norshariza, Yaakub, Halimatun, Nasr Esfahani, Mohammad Hosein

“…Real-time PCR analysis of gene expression revealed significantly higher expression of POU5F1 in cloned versus IVF blastocysts. Neither embryo production method (SCNT vs. …”
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