Improved in vitro development of cloned bovine embryos using S-adenosylhomocysteine, a non-toxic epigenetic modifying reagent. by Jafari, Shahram, Hosseini, Morteza S., Hajian, Mehdi, Forouzanfar, Mohsen, Jafarpour, Farnoosh, Abedi, Parvaneh, Ostadhosseini, Somayyeh, Abbasi, Hassan, Gourabi, Hamid, Shahverdi, Abdolhossein H., Dizaj, A. V. T., Anjomshoaa, Maryam, Haron, Wahid, Noor, Noorshariza, Yakub, Halimhatoon, Nasr Esfahani, Mohammad Hosein

“…Real-time PCR analysis of gene expression revealed significantly higher expression of POU5F1 in cloned versus IVF blastocysts. Neither embryo production method (SCNT vs. …”
Get full text

Identification of Differentially Methylated Regions (DMRs) between Embryonic Stem Cells (ESCs) and Embryonic Germ Cells (ESCs) by DNA methylation studies by Lynn, Khoo Jo

“…ESCs are pluripotent stem cells derived from inner cell mass (ICM) of pre–implantation embryos called blastocyst, while EGCs are pluripotent stem cells originated from primordial germ cells (PGCs). …”
Get full text

Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos by Weikai Ge, Xiaozhu Zhao, Shixue Gou, Qin Jin, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu, Kexin Zhong, Yanhui Liang, Tao Chen, Han Wu, Nan Li, Yinghua Ye, Liangxue Lai, Kepin Wang

“…Here, using pig embryos as subjects, we observed that Cas9 protein transcribed from injected Cas9 mRNA can persist until at least the blastocyst stage. Cas9 protein alone can induce genome damage in preimplantation embryos, represented by the increased number of phosphorylated histone H2AX foci on the chromatin fiber, which led to apoptosis and decreased cell number of blastocysts. …”
Get full text

Single-embryo transcriptomic atlas of oxygen response reveals the critical role of HIF-1α in prompting embryonic zygotic genome activation by Fusheng Yao, Meiqiang Chu, Guangyin Xi, Jiage Dai, Zhaochen Wang, Jia Hao, Qianying Yang, Wenjing Wang, Yawen Tang, Jingyu Zhang, Yuan Yue, Yue Wang, Yefen Xu, Wei Zhao, Lizhu Ma, Juan Liu, Zhenni Zhang, Jianhui Tian, Lei An

“…In addition, we found atmosO2 impedes metabolic shift to glycolysis before blastocyst formation, thus resulting a low-level histone lactylation deposition. …”
Get full text

The Resurrection of Mabrokan: Production of Multiple Cloned Offspring from Decade-Old Vitrified Tissue Collected from a Deceased Champion Show Camel by Mohammad Shamim Hossein, Xianfeng Yu, Young-Bum Son, Yeon-Ik Jeong, Yeon-Woo Jeong, Eun-Ji Choi, Alex H. Tinson, Kuhad Kuldip Singh, Rajesh Singh, Al Shamsi Noura, Woo-Suk Hwang

“…We observed a higher blastocyst formation rate when in vivo matured oocytes (43.45 ± 2.07%) were used compared to in vitro matured oocytes (21.52 ± 1.74%). …”
Get full text

Isolation, characterization, proteome, miRNAome, and the embryotrophic effects of chicken egg yolk nanovesicles (vitellovesicles) by Islam M. Saadeldin, Bereket Molla Tanga, Seonggyu Bang, Chaerim Seo, Abdulkadir Y. Maigoro, Heejae Kang, Dabin Cha, Sung Ho Yun, Seung Il Kim, Sanghoon Lee, Jongki Cho

“…VVs were supplemented with the in vitro culture medium of day-7 hatched parthenogenetic blastocysts. After 2 days of blastocyst culture, the embryonic cell count was increased in VVs supplemented embryos in comparison to the non-supplemented embryos. …”
Get full text

Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility by YuSheng Zhang, YaNan Liu, Zi Teng, ZeLin Wang, Peng Zhu, ZhiXin Wang, FuJun Liu, XueXia Liu

“…In IVF experiments, PTX impaired sperm fertility and blastocyst development, but hUC-MSCs treatment rescued these indicators. hUC-MSCs’ protective role was also displayed through the increased expression of the fertility-related proteins HSPA2 and HSPA4L in testes with decreased expression in the PTX-treated group. …”
Get full text

Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls by Anna Lange-Consiglio, Emanuele Capra, Noemi Monferini, Simone Canesi, Giampaolo Bosi, Marina Cretich, Roberto Frigerio, Valentina Galbiati, Federica Bertuzzo, Francesco Cobalchini, Fausto Cremonesi, Bianca Gasparrini

“…Subsequently, the spermatozoa of low-fertility bulls, with EVs incorporated, were used for the in vitro production of embryos. The rate of blastocyst at seventh day yield in vitro, with the use of sperm with EVs incorporated, increased by about twice the yield obtained with the same sperm in the absence of EVs: bulls having an average embryonic yield of 6.41 ± 1.48%, 10.32 ± 4.34%, and 10.92 ± 0.95% improved their yield to 21.21 ± 1.99%, 22.17 ± 6.09%, and 19.99 ± 5.78%, respectively (P < 0.05). …”
Get full text

Cleavage Patterns of 9600 Embryos: The Importance of Irregular Cleavage by Maya Shavit, Daniel Gonen, Yuval Atzmon, Nardin Aslih, Asaf Bilgory, Yasmin Shibli Abu-Raya, Moamina Sharqawi, Daniela Estrada Garcia, Mediea Michaeli, Diana Polotov, Einat Shalom-Paz

“…Frozen transfer cycles of blastocyst embryos, with or without IRC, had comparable results. …”
Get full text

Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation by Daniel Angel-Velez, Tim Meese, Mohamed Hedia, Andrea Fernandez-Montoro, Tine De Coster, Osvaldo Bogado Pascottini, Filip Van Nieuwerburgh, Jan Govaere, Ann Van Soom, Krishna Pavani, Katrien Smits

“…In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. …”
Get full text

Autologous Platelet-Rich Plasma (PRP) Efficacy on Endometrial Thickness and Infertility: A Single-Centre Experience from Romania by Anca Huniadi, Ioana Alexandra Zaha, Petronela Naghi, Liana Stefan, Liliana Sachelarie, Alin Bodog, Erika Szuhai-Bimbo, Codruta Macovei, Mircea Sandor

“…The control group included 51 patients with at least one attempt to transfer a good-quality blastocyst, but the endometrial thickness did not surpass 7 mm under standard endometrial preparation protocol with oestradiol and with adjuvant therapy (other than PRP, such as aspirin, vitamin C, and vitamin E), and the study group included the same 51 patients that had the embryo transfer performed under the same standard endometrial preparation protocol with oestradiol preparation protocol and intrauterine PRP infusion. (3) <i>Results</i>: In our study, the PRP treatment had a positive impact on the parameters that were followed for the evaluation of the success rate of the embryo transfer procedure. …”
Get full text

Double cytoplast embryonic cloning improves in vitro but not in vivo development from mitotic pluripotent cells in cattle by Sarah Jane Appleby, Sarah Jane Appleby, Pavla Misica‐Turner, Fleur Catherine Oback, Arindam Dhali, Zachariah Louis McLean, Zachariah Louis McLean, Björn Oback, Björn Oback, Björn Oback

“…Sequential double cytoplast ECT, whereby another cytoplast was fused to the first cloned reconstruct, doubled cloned blastocyst development and improved morphological embryo quality. …”
Get full text

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine <i>miR-17-92</i> Cluster by Chao Lu, Daxin Pang, Mengjing Li, Hongming Yuan, Tingting Yu, Peixuan Huang, Jianing Li, Xue Chen, Huping Jiao, Zicong Xie, Hongsheng Ouyang

“…Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous p<i>miR-17-92</i> expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. …”
Get full text

Biology of Perseverative Negative Thinking: The Role of Timing and Folate Intake by Nora Eszlari, Bence Bruncsics, Andras Millinghoffer, Gabor Hullam, Peter Petschner, Xenia Gonda, Gerome Breen, Peter Antal, Gyorgy Bagdy, John Francis William Deakin, Gabriella Juhasz

“…Furthermore, biological pathways leading to rumination appeared to differ according to folate intake: purinergic signaling and circadian regulator gene <i>ARNTL</i> emerged in the whole sample, blastocyst development, DNA replication, and C-C chemokines in the suboptimal folate group, and prostaglandin response and K⁺ channel subunit gene <i>KCNH3</i> in the optimal folate group. …”
Get full text

Butylparaben Is Toxic to Porcine Oocyte Maturation and Subsequent Embryonic Development Following In Vitro Fertilization by Pil-Soo Jeong, Sanghoon Lee, Soo-Hyun Park, Min Ju Kim, Hyo-Gu Kang, Tsevelmaa Nanjidsuren, Hee-Chang Son, Bong-Seok Song, Deog-Bon Koo, Bo-Woong Sim, Sun-Uk Kim

“…The 300 μM BP significantly decreased fertilization, cleavage, and blastocyst formation rates with lower total cell numbers and a higher rate of apoptosis in blastocysts compared to the control. …”
Get full text

Are Clinical Outcomes of Micro-TESE in Non-obstructive Azoospermic Men Affected by the Use of Fresh or Frozen Gametes? by Fabrizzio Horta, Dhanushi Fernando, Daniel Lantsberg, Sandra Holden, Darren J. Katz, Mark P. Green, Robert McLachlan, Deirdre Zander-Fox, Luk Rombauts

“…The impact of gamete fresh/frozen status and sperm motility was investigated on clinical outcomes such as fertilization, blastocyst formation, clinical pregnancy, and live birth rates (LBRs). …”
Get full text

The association between systemic immune-inflammation index and in vitro fertilization outcomes in women with polycystic ovary syndrome: a cohort study by Xin Li, Ting Luan, Yi Wei, Juan Zhang, JuanJuan Zhang, Chun Zhao, Xiufeng Ling

“…Moreover, our analysis demonstrated that women in the lower SII quartiles had a higher rate of available embryos and blastocyst formation compared with those in the highest SII quartile. …”
Get full text

6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress by Wenjie Yu, Yanxia Peng, Xinyue Peng, Ze Li, Chang Liu, Liu Yang, Yan Gao, Shuang Liang, Bao Yuan, Chengzhen Chen, Nam-hyung Kim, Hao Jiang, Jiabao Zhang

“…The results showed that 5 μM 6-gingerol significantly increased the blastocyst formation rates of porcine early embryos. 6-Gingerol attenuated intracellular reactive oxygen species accumulation and autophagy, increased intracellular glutathione levels, and increased mitochondrial activity. …”
Get full text

BDNF promotes mouse follicular development and reverses ovarian aging by promoting cell proliferation by Bin Liu, Yongjie Liu, Shuman Li, Pingping Chen, Jun Zhang, Liping Feng

“…Results rhBDNF treatment increased the ovarian weight, number of follicles, number and quality of oocytes including increased blastocysts formation, blood estrogen levels, and pregnancy rate in 35-37-week-old mice. …”
Get full text

Cell Cycle Stage and DNA Repair Pathway Influence CRISPR/Cas9 Gene Editing Efficiency in Porcine Embryos by Karina Gutierrez, Werner G. Glanzner, Mariana P. de Macedo, Vitor B. Rissi, Naomi Dicks, Rodrigo C. Bohrer, Hernan Baldassarre, Luis B. Agellon, Vilceu Bordignon

“…However, gene editing was higher in blastocysts from late-(73.8%) vs. early-(63.8%) injected zygotes. …”
Get full text