MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice by Jonathan Vásquez Cavero, Pilar Pino Velásquez, José Gonzáles Daga, Eduardo Pulido-Murillo, José L. Pino Gaviño

“…However, no significant differences were found in the percentage of blastocyst stage embryos, good quality embryos, or embryos with greater than 50% the size of the embryo between the two groups (p> 0.05) blastoceles. …”
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Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrification by A. Habibi, N. Farrokhi, F. Morreira da Silva, A. Hosseini, B.F. Bettencourt, F. Amidi

“…The relative expression of Hsp72 was the same in vit1 and vit2 and significantly higher than in the control.The survival, cleavage, and blastocyst rates were the same for both vitrification treatments and significantly lower than in the control group. …”
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The regulation and function of acetylated high-mobility group box 1 during implantation and decidualization by Yue Li, Yue Li, Si-Ting Chen, Yu-Ying He, Bo Li, Chen Yang, Zhen-Shan Yang, Zeng-Ming Yang, Zeng-Ming Yang

“…There is an obvious difference for secreted HMGB1 levels in uterine fluid between day 4 of pregnancy and day 4 of pseudopregnancy, suggesting the involvement of blastocysts during HMGB1 secretion. Trypsin is clearly detected in mouse blastocyst cavity and in the supernatant of cultured blastocysts. …”
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Identification of Important Factors Causing Developmental Arrest in Cloned Pig Embryos by Embryo Biopsy Combined with Microproteomics by Yuxing Zhang, Liusong Yang, Yiqian Zhang, Yalin Liang, Huaxing Zhao, Yanan Li, Gengyuan Cai, Zhenfang Wu, Zicong Li

“…<i>PDCD6</i> knockdown also increased the cleavage rate and blastocyst rate of cloned porcine embryos. Overexpression of <i>PLK1</i> via mRNA microinjection also improved the cleavage rate of cloned pig embryos. …”
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Telomere Length in Human Spermatogenic Cells as a New Potential Predictor of Clinical Outcomes in ART Treatment with Intracytoplasmic Injection of Testicular Spermatozoa by Anna A. Pendina, Mikhail I. Krapivin, Yanina M. Sagurova, Irina D. Mekina, Evgeniia M. Komarova, Andrei V. Tikhonov, Arina V. Golubeva, Alexander M. Gzgzyan, Igor Yu. Kogan, Olga A. Efimova

“…The analysis of embryological outcomes revealed a dependence between embryo competence for development to the blastocyst stage and the TL in spermatogenic cells. …”
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Stage-Specific L-Proline Uptake by Amino Acid Transporter Slc6a19/B⁰AT1 Is Required for Optimal Preimplantation Embryo Development in Mice by Tamara Treleaven, Matthew Zada, Rajini Nagarajah, Charles G. Bailey, John E. J. Rasko, Michael B. Morris, Margot L. Day

“…In WT embryos, Pro increased development to the cavitation stage (on day 4); whereas in B⁰AT1 knockout embryos Pro improved development to the 5–8-cell (day 3) and blastocyst stages (day 6) but not at cavitation (day 4), suggesting B⁰AT1 is the main contributor to Pro uptake on day 4 of development. …”
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Time-Lapse Incubation for Embryo Culture-Morphokinetics and Environmental Stability May Not Be Enough: Results from a Pilot Randomized Controlled Trial by Gilat C. Sacks, Henny Mozes, Ruth Ronn, Talia Elder-Geva, Oshrat Schonberger, Ido Ben-Ami, Naama Srebnik

“…Comparable pregnancy rates, clinical pregnancy rates, and cumulative pregnancy were found for similar top-quality embryos for days 2, 3, and blastocyst stages across groups. A similar number of embryos were suitable for either transfer or cryopreservation within the different groups. …”
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Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation by Constance E. Clare, Valerie Pestinger, Wing Yee Kwong, Desmond A. R. Tutt, Juan Xu, Helen M. Byrne, David A. Barrett, Richard D. Emes, Kevin D. Sinclair

“…Here we report that transcripts for all bar two genes (i.e., <i>BHMT</i>, <i>MAT1A</i>) encoding enzymes in the linked methionine-folate cycles are expressed in all cell types within the ovarian follicle, oocyte, and blastocyst in the cow, sheep, and pig; as well as in rat granulosa cells (GCs) and human KGN cells (a granulosa-like tumor cell line). …”
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Uterine Nodal expression supports maternal immunotolerance and establishment of the FOXP3+ regulatory T cell population during the preimplantation period by Sarah Yull, Sarah Yull, Shiva Shafiei, Shiva Shafiei, Craig B. Park, Parinaz Kazemi, Parinaz Kazemi, Emily B. Tiemann, Marie-Hélène Godin Pagé, Marie-Hélène Godin Pagé, Daniel Dufort, Daniel Dufort, Daniel Dufort, Daniel Dufort

“…The immunosuppressive function of regulatory T cells is critical to limit inflammation arising from implantation of the semi-allogeneic blastocyst. Insufficient maternal immune adaptations to pregnancy have been frequently associated with cases of female infertility and recurrent implantation failure. …”
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Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos by Pil-Soo Jeong, Hae-Jun Yang, Se-Been Jeon, Min-Ah Gwon, Min Ju Kim, Hyo-Gu Kang, Sanghoon Lee, Young-Ho Park, Bong-Seok Song, Sun-Uk Kim, Deog-Bon Koo, Bo-Woong Sim

“…After parthenogenetic activation or SCNT, the developmental competence of Lut-supplemented MII oocytes was significantly enhanced, as indicated by higher rates of cleavage, blastocyst formation, expanded or hatching blastocysts, and cell survival, as well as increased cell numbers. …”
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Gonadotropin-releasing hormone agonist downregulation combined with hormone replacement therapy improves the reproductive outcome in frozen–thawed embryo transfer cycles for patien... by Dan Pan, Jie Yang, Ni Zhang, Lei Wang, Na Li, Juanzi Shi, Hanying Zhou

“…Methods This retrospective cohort study involved 549 older patients undergoing their third cleavage-stage embryo or blastocyst transfer over a 5-year period (January 2015–December 2020) at Northwest Women’s and Children’s Hospital after in vitro fertilization/intracytoplasmic sperm injection cycles. …”
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The Immune Profile of the Endometrium in the "Uterine Factor" of Infertility by Miroslava L. Polina, Victor E. Radzinsky, Ludmila M. Mikhaleva, Irina I. Vityazeva, Marina B. Khamoshina, Sergey А. Mikhalev, Natalya I. Douglas

“…Aberrant expression of immune factors may contribute to the formation of a microenvironment unfavorable for blastocyst implantation.…”
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Assisted Reproductive Technique and Abnormal Cord Insertion: A Systematic Review and Meta-Analysis by Shinya Matsuzaki, Yutaka Ueda, Satoko Matsuzaki, Yoshikazu Nagase, Mamoru Kakuda, Misooja Lee, Michihide Maeda, Hiroki Kurahashi, Harue Hayashida, Tsuyoshi Hisa, Seiji Mabuchi, Shoji Kamiura

“…Nevertheless, the VCI rate was similar among the different ART types (blastocyst <i>versus</i> cleavage-stage transfer and frozen <i>v</i><i>ersus</i> fresh embryo transfer). …”
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Pterostilbene alleviates hydrogen peroxide-induced oxidative stress via nuclear factor erythroid 2 like 2 pathway in mouse preimplantation embryos by Obaid ULLAH, Zhongshu LI, Ihsan ALI, Lijie XU, Haixing LIU, Syed Zahid Ali SHAH, Nanzhu FANG

“…Treatment of zygotes with 0.25 μM PTS improved the development of day 4 blastocysts (P &lt; 0.05). Moreover, H2O2 treatment significantly increased the reactive oxygen species level and reduced the glutathione level in mouse blastocyst, whereas PTS treatment counteracted these effects. …”
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Cervical Secretion Methylation Is Associated with the Pregnancy Outcome of Frozen-Thawed Embryo Transfer by Yi-Xuan Lee, Po-Hsuan Su, Anh Q. Do, Chii-Ruei Tzeng, Yu-Ming Hu, Chi-Huang Chen, Chien-Wen Chen, Chi-Chun Liao, Lin-Yu Chen, Yu-Chun Weng, Hui-Chen Wang, Hung-Cheng Lai

“…Cervical secretions were collected from women who entered the FET cycle with a blastocyst transfer (36 pregnant and 36 non-pregnant women). …”
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Serum uric acid did not affect embryonic and pregnancy outcomes in women without PCOS during IVF procedures by Niwei Yan, Niwei Yan, Niwei Yan, Junli Song, Junli Song, Huiying Jie, Huiying Jie, Pingyin Lee, Pingyin Lee, Simin Liu, Simin Liu, Yuan Yuan, Yuan Yuan

“…Multivariable linear regression and binary regression were applied to detect the association between IVF outcomes and serum uric acid levels.ResultsThe number of retrieved oocytes, fertilization rate, viable embryo rate, blastocyst formation rate and euploid rate were not associated with serum uric acid levels. …”
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#165 : Application of a Highly Reliable Protocol of Non-Invasive Preimplantation Genetic Testing for IVF Cycles and Its Clinical Outcome by Yen-An Tang, I-Ning Huang, Shiow-Jan Lee, H. Sunny Sun, Hsien-An Pan

“…Methods: Three hundred and sixty-two blastocysts from 94 patients undergoing IVF between January 2021 and August 2022 were analyzed. …”
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Melatonin, Its Beneficial Effects on Embryogenesis from Mitigating Oxidative Stress to Regulating Gene Expression by Dmitry Ivanov, Gianluigi Mazzoccoli, George Anderson, Natalia Linkova, Anastasiia Dyatlova, Ekaterina Mironova, Victoria Polyakova, Igor Kvetnoy, Inna Evsyukova, Annalucia Carbone, Ruslan Nasyrov

“…MT activates the expression of the <i>ErbB1, ErbB4, GJA1, POU5F1,</i> and <i>Nanog</i> genes which are necessary for embryo implantation and blastocyst growth. MT induces the expression of vascular endothelial growth factor (VEGF) and its type 1 receptor (VEGF-R1) in the ovaries, activating angiogenesis. …”
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Effectiveness of oestrogen pretreatment in patients with expected poor ovarian response (POSEIDON groups 3 and 4) undergoing GnRH antagonist protocol: study protocol for a randomis... by Yue Zhou, Qiao-Song Han, Zhi-Juan Wu, Jing-Nan Liang, Qing-Mei Jin, Jing-Yan Song, Zhen-Gao Sun

“…Secondary outcomes include the total number of retrieved metaphase II oocytes, average daily dose of gonadotropin, total gonadotropin dose and duration of ovarian stimulation, cycle cancellation rate, top quality embryos rate, blastocyst formation rate, embryo implantation rate, clinical pregnancy rate, early miscarriage rate and endometrial thickness on trigger day. …”
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The role of elagolix in the suppression of ovulation in donor oocyte cycles by Catherine Boniface, M.D., Jessica N. Schnorr, M.D., Jeff Gray, Ph.D., Jessica McLaughlin, M.D., Heather Cook, M.D., Michael Slowey, M.D., John Schnorr, M.D.

“…An analysis of the 580 fresh oocytes in the elagolix group and the 737 fresh oocytes in the ganirelix group showed similar outcomes with fertilization rates of 79.7% and 84.6%, respectively. Blastocyst development rates were also similar: 62.9% in the elagolix group and 57.3% in the ganirelix group. …”
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