Assessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics by Bastien Ducreux, Julie Barberet, Julie Barberet, Magali Guilleman, Magali Guilleman, Raquel Pérez-Palacios, Aurélie Teissandier, Déborah Bourc’his, Patricia Fauque, Patricia Fauque

“…Overall, the differences were reduced at the blastocyst stage, highlighting the ability of embryos conceived in a suboptimal in vitro culture medium to mitigate the transcriptomic profile acquired under different pre-compaction environments.…”
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Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation by Kevin C. L. Cheng, Jennifer M. Frost, Francisco J. Sánchez-Luque, Marta García-Canãdas, Darren Taylor, Wan R. Yang, Branavy Irayanar, Swetha Sampath, Hemalvi Patani, Karl Agger, Kristian Helin, Gabriella Ficz, Kathleen H. Burns, Adam Ewing, José L. García-Pérez, Miguel R. Branco

“…The epigenetic remodelling promoted by vitC improves the efficiency of induced pluripotent stem cell derivation, and is required to attain a ground-state of pluripotency in embryonic stem cells (ESCs) that closely mimics the inner cell mass of the early blastocyst. However, genome-wide DNA and histone demethylation can lead to upregulation of transposable elements (TEs), and it is not known how vitC addition in culture media affects TE expression in pluripotent stem cells. …”
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Effect of D-Glucuronic Acid and N-acetyl-D-Glucosamine Treatment during In Vitro Maturation on Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs by Joohyeong Lee, Eunhye Kim, Seon-Ung Hwang, Lian Cai, Mirae Kim, Hyerin Choi, Dongjin Oh, Eunsong Lee, Sang-Hwan Hyun

“…In both the chemically defined and the complex medium (Medium-199 + porcine follicular fluid), oocytes derived from the GA and AG treatments presented significantly higher blastocyst rates than the control after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). …”
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Higher Doses of FSH Used for Superovulation Do Not Adversely Affect Embryonic Ploidy: A Randomised Controlled Trial (Stimulation Resulting in Embryonic Aneuploidy Using Menopur (ST... by Akshara SHYAMSUNDER, Tristan HARDY, Anusch YAZDANI, Alex POLYAKOV, Robert NORMAN, Roger HART, Franca AGRESTA, Luk ROMBAUTS, Clare BOOTHROYD, Michael CHAPMAN, Prudence SWEETEN, Eleanor SOMERVILLE, Rachel JOSE, Handan WAND, William L. LEDGER

“…Aim: To determine the proportion of euploid blastocysts resulting from higher versus lower dose ovarian stimulation protocols. …”
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Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19 by Raquel Bernad, Cian J. Lynch, Rocio G. Urdinguio, Camille Stephan-Otto Attolini, Mario F. Fraga, Manuel Serrano

“…Naïve pluripotent stem cells (PSCs) correspond to the early pre-implantation blastocyst and, in mice, constitute the optimal starting state for subsequent developmental applications. …”
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Dicer deletion and short RNA expression analysis in mouse embryonic stem cells by Calabrese, Joseph Mauro

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Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency by Ai, Zhipeng, Xiang, Xinyu, Xiang, Yangquan, Szczerbinska, Iwona, Qian, Yuli, Xu, Xiao, Ma, Chenyang, Su, Yaqi, Gao, Bing, Shen, Hao, Muhammad Nadzim Bin Ramli, Chen, Di, Liu, Yue, Hao, Jia-Jie, Ng, Huck Hui, Zhang, Dan, Chan, Yun-Shen, Liu, Wanlu, Liang, Hongqing

“…Here, we demonstrate the mechanism of how LTR7Ys, the human morula-blastocyst-specific HERVH long terminal repeats, are activated by the naive pluripotency transcription network. …”
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Characterisation of AEBP2: a polycomb repressive complex 2 component by Grijzenhout, A

“…Additionally, these cells have been used for blastocyst injection to create an <em>Aebp2</em> gene-trapped mouse. …”

Exogenous L-glutathione improves vitrification outcomes in murine preimplantation embryos by Abdul Rahman, Nor Shahida, Mohamed Noor Khan, Nor Ashikin, Eshak, Zolkapli, Sarbandi, Mimi Sophia, Mohammad Kamal, Aqila Akmal, Abd Malek, Mastura, Abdullah, Fathiah, Abdullah, Maizaton Atmadini, Othman, Fezah

“…In the non-vitrified groups, there were significant increases in the number of Grade-1 blastocysts in GSH cultures (p < 0.05). Similarly, in the vitrified groups, GSH supplementation was also seen to significantly increase blastocyst formation. …”

Essays on Developmental Biology. by Wassarman, Paul M., editor

Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development by Yazheng Dong, Xiao Wu, Xitong Peng, Liusong Yang, Baohua Tan, Huaxing Zhao, Enqin Zheng, Linjun Hong, Gengyuan Cai, Zhenfang Wu, Zicong Li

“…The microinjection of YY1 siRNA into cloned pig embryos reduced the transcript abundance of <i>XIST</i> and upregulated the mRNA level of X-linked genes at the 4-cell and blastocyst stages. The siRNA-mediated knockdown of YY1 altered the transcriptome and enhanced the in vitro and in vivo developmental efficiency of cloned porcine embryos. …”
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Association of ADAMTS proteoglycanases downregulation with IVF-ET outcomes in patients with polycystic ovary syndrome: a systematic review and meta-analysis by Yanbin Shi, Yang Shi, Guiyuan He, Guang Wang, Hongbo Liu, Xiaoguang Shao

“…Finally, the oocytes recovery rate, oocyte maturity rate, fertilization rate, cleavage rate, good-quality embryos rate, blastocyst formation rate, and clinical pregnancy rate were not affected by the downregulation of ADAMTS-1, ADAMTS-4, ADAMTS-5, and ADAMTS-9 (P > 0.05). …”
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Assisted gamete treatment to pinpoint acquired meiotic maturity and overcome oocyte activation deficiency contributed by both gametes by Philip Xie, B.Sc., Ashley Aluko, M.D., Stephanie Cheung, M.Sc., Dan Goldschlag, M.D., Owen Davis, M.D., Zev Rosenwaks, M.D., Gianpiero D. Palermo, M.D., Ph.D.

“…Couple 2 underwent 3 ICSI cycles with EOM and AGT; 91.4% (32/35) of oocytes displayed normal metaphase II spindle and achieved an overall fertilization rate of 43.8% (14/32). A total of 12 blastocysts were cryopreserved. A single 46XY blastocyst was thawed and transferred, resulting in a singleton pregnancy. …”
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Hepatitis B Infection Negatively Affects the Outcome of Fresh IVF/ICSI Cycles by Patricia Petric, Tadej Fevzer, Nina Jancar, Eda Vrtacnik-Bokal, Martin Stimpfel

“…The number of obtained embryos, blastocyst, and embryo utilization rates were similar between the groups, although the rate of cryopreserved embryos was higher in the HF (p = 0.007) and the HM groups (p = 0.027) compared to the control group. …”
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Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning by Ming-Zhu Sun, Yao-Wei Liu, Mao-Sheng Cui, Qi-Li Zhao, Xiang-Fei Zhao, Yi-Di Zhang, Jing-Jing Huang, Gui-Zhang Lu, Xin Zhao

“…Experimental results showed that we increased the blastocyst rate from 10.0% to 20.8%, and we successfully produced 17 cloned piglets by robotic SCNT for the first time. …”
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Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study by Rui Fang, Weimin Yang, Xin Zhao, Fang Xiong, Caiqing Guo, Jianping Xiao, Li Chen, Xiaoqing Song, Honghua Wang, Jie Chen, Xiao Xiao, Bing Yao, Li-Yi Cai

“…NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. …”
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Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis by Hyo-Gu Kang, Sanghoon Lee, Pil-Soo Jeong, Min Ju Kim, Soo-Hyun Park, Ye Eun Joo, Sung Hyun Park, Bong-Seok Song, Sun-Uk Kim, Min Kyu Kim, Bo-Woong Sim

“…In addition, Day-2 embryos and Day-6 blastocysts treated with 0.1 μM lycopene showed significantly reduced mRNA expression related to antioxidant enzymes (<i>SOD1, SOD2, CATALASE</i>) and apoptosis (<i>BAX/BCL2L1</i> ratio). …”
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Nomogram for predicting the risk of preterm delivery after IVF/ICSI treatment: an analysis of 11513 singleton births by Zhiqi Liao, Lei Cai, Chang Liu, Jie Li, Xinyao Hu, Youhua Lai, Lin Shen, Cong Sui, Hanwang Zhang, Kun Qian

“…Receiver operating characteristic curves (ROC), areas under the ROC curves (AUC), concordance index (C-index), and calibration plots were analyzed for assessing the performance of this nomogram in the training and validation group.ResultsThere were fourteen risk factors significantly related to PTD in IVF/ICSI singleton live births, including maternal body mass index (BMI) &gt; 24 kg/m2, smoking, uterine factors, cervical factors, ovulatory factors, double embryo transferred (DET), blastocyst transfer, FET, vanishing twin syndrome (VTS), obstetric complications (placenta previa, placenta abruption, hypertensive of pregnancies, and premature rupture of membrane), and a male fetus. …”
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Events Leading to the Establishment of Pregnancy and Placental Formation: The Need to Fine-Tune the Nomenclature on Pregnancy and Gestation by Giuseppe Benagiano, Salvatore Mancuso, Sun-Wei Guo, Gian Carlo Di Renzo

“…This intense molecular cross-talk between the early embryo and the endometrium starts even before the blastocyst reaches the uterine cavity, substantially initiating and conditioning the process of implantation and the formation of the placenta. …”
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Antioxidant Nobiletin Enhances Oocyte Maturation and Subsequent Embryo Development and Quality by Yulia N. Cajas, Karina Cañón-Beltrán, Magdalena Ladrón de Guevara, María G. Millán de la Blanca, Priscila Ramos-Ibeas, Alfonso Gutiérrez-Adán, Dimitrios Rizos, Encina M. González

“…Similarly, cleavage rate and cumulative blastocyst yield on Days 7 and 8 were significantly higher for Nob25 and Nob50 groups. …”
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