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1401
Assessing the influence of distinct culture media on human pre-implantation development using single-embryo transcriptomics
Published 2023-06-01“…Overall, the differences were reduced at the blastocyst stage, highlighting the ability of embryos conceived in a suboptimal in vitro culture medium to mitigate the transcriptomic profile acquired under different pre-compaction environments.…”
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1402
Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation
Published 2023-10-01“…The epigenetic remodelling promoted by vitC improves the efficiency of induced pluripotent stem cell derivation, and is required to attain a ground-state of pluripotency in embryonic stem cells (ESCs) that closely mimics the inner cell mass of the early blastocyst. However, genome-wide DNA and histone demethylation can lead to upregulation of transposable elements (TEs), and it is not known how vitC addition in culture media affects TE expression in pluripotent stem cells. …”
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1403
Effect of D-Glucuronic Acid and N-acetyl-D-Glucosamine Treatment during In Vitro Maturation on Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
Published 2021-04-01“…In both the chemically defined and the complex medium (Medium-199 + porcine follicular fluid), oocytes derived from the GA and AG treatments presented significantly higher blastocyst rates than the control after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). …”
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1404
Higher Doses of FSH Used for Superovulation Do Not Adversely Affect Embryonic Ploidy: A Randomised Controlled Trial (Stimulation Resulting in Embryonic Aneuploidy Using Menopur (ST...
Published 2022-09-01“…Aim: To determine the proportion of euploid blastocysts resulting from higher versus lower dose ovarian stimulation protocols. …”
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1405
Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19
Published 2021-04-01“…Naïve pluripotent stem cells (PSCs) correspond to the early pre-implantation blastocyst and, in mice, constitute the optimal starting state for subsequent developmental applications. …”
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1406
Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
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Thesis -
1407
Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency
Published 2023“…Here, we demonstrate the mechanism of how LTR7Ys, the human morula-blastocyst-specific HERVH long terminal repeats, are activated by the naive pluripotency transcription network. …”
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Journal Article -
1408
Characterisation of AEBP2: a polycomb repressive complex 2 component
Published 2013“…Additionally, these cells have been used for blastocyst injection to create an <em>Aebp2</em> gene-trapped mouse. …”
Thesis -
1409
Exogenous L-glutathione improves vitrification outcomes in murine preimplantation embryos
Published 2022“…In the non-vitrified groups, there were significant increases in the number of Grade-1 blastocysts in GSH cultures (p < 0.05). Similarly, in the vitrified groups, GSH supplementation was also seen to significantly increase blastocyst formation. …”
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1410
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1411
Knockdown of YY1 Inhibits <i>XIST</i> Expression and Enhances Cloned Pig Embryo Development
Published 2022-11-01“…The microinjection of YY1 siRNA into cloned pig embryos reduced the transcript abundance of <i>XIST</i> and upregulated the mRNA level of X-linked genes at the 4-cell and blastocyst stages. The siRNA-mediated knockdown of YY1 altered the transcriptome and enhanced the in vitro and in vivo developmental efficiency of cloned porcine embryos. …”
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1412
Association of ADAMTS proteoglycanases downregulation with IVF-ET outcomes in patients with polycystic ovary syndrome: a systematic review and meta-analysis
Published 2022-12-01“…Finally, the oocytes recovery rate, oocyte maturity rate, fertilization rate, cleavage rate, good-quality embryos rate, blastocyst formation rate, and clinical pregnancy rate were not affected by the downregulation of ADAMTS-1, ADAMTS-4, ADAMTS-5, and ADAMTS-9 (P > 0.05). …”
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1413
Assisted gamete treatment to pinpoint acquired meiotic maturity and overcome oocyte activation deficiency contributed by both gametes
Published 2023-03-01“…Couple 2 underwent 3 ICSI cycles with EOM and AGT; 91.4% (32/35) of oocytes displayed normal metaphase II spindle and achieved an overall fertilization rate of 43.8% (14/32). A total of 12 blastocysts were cryopreserved. A single 46XY blastocyst was thawed and transferred, resulting in a singleton pregnancy. …”
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1414
Hepatitis B Infection Negatively Affects the Outcome of Fresh IVF/ICSI Cycles
Published 2022-07-01“…The number of obtained embryos, blastocyst, and embryo utilization rates were similar between the groups, although the rate of cryopreserved embryos was higher in the HF (p = 0.007) and the HM groups (p = 0.027) compared to the control group. …”
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1415
Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning
Published 2023-05-01“…Experimental results showed that we increased the blastocyst rate from 10.0% to 20.8%, and we successfully produced 17 cloned piglets by robotic SCNT for the first time. …”
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1416
Chromosome screening using culture medium of embryos fertilised in vitro: a pilot clinical study
Published 2019-03-01“…NICS was carried out using embryo culture medium at the blastocyst stage via multiple annealing and looping-based amplification cycles, whole-genome amplification and next-generation sequencing. …”
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1417
Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis
Published 2021-02-01“…In addition, Day-2 embryos and Day-6 blastocysts treated with 0.1 μM lycopene showed significantly reduced mRNA expression related to antioxidant enzymes (<i>SOD1, SOD2, CATALASE</i>) and apoptosis (<i>BAX/BCL2L1</i> ratio). …”
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1418
Nomogram for predicting the risk of preterm delivery after IVF/ICSI treatment: an analysis of 11513 singleton births
Published 2023-05-01“…Receiver operating characteristic curves (ROC), areas under the ROC curves (AUC), concordance index (C-index), and calibration plots were analyzed for assessing the performance of this nomogram in the training and validation group.ResultsThere were fourteen risk factors significantly related to PTD in IVF/ICSI singleton live births, including maternal body mass index (BMI) > 24 kg/m2, smoking, uterine factors, cervical factors, ovulatory factors, double embryo transferred (DET), blastocyst transfer, FET, vanishing twin syndrome (VTS), obstetric complications (placenta previa, placenta abruption, hypertensive of pregnancies, and premature rupture of membrane), and a male fetus. …”
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1419
Events Leading to the Establishment of Pregnancy and Placental Formation: The Need to Fine-Tune the Nomenclature on Pregnancy and Gestation
Published 2023-10-01“…This intense molecular cross-talk between the early embryo and the endometrium starts even before the blastocyst reaches the uterine cavity, substantially initiating and conditioning the process of implantation and the formation of the placenta. …”
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1420
Antioxidant Nobiletin Enhances Oocyte Maturation and Subsequent Embryo Development and Quality
Published 2020-07-01“…Similarly, cleavage rate and cumulative blastocyst yield on Days 7 and 8 were significantly higher for Nob25 and Nob50 groups. …”
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Article