Artificial oocyte activation with Ca2+ ionophore improves reproductive outcomes in patients with fertilization failure and poor embryo development in previous ICSI cycles by Jing Ling Ruan, Jing Ling Ruan, Shan Shan Liang, Shan Shan Liang, Jia Ping Pan, Jia Ping Pan, Zhi Qin Chen, Xiao Ming Teng

“…The fertilization rates, cleavage rates, transferable embryo rates and blastocyst formation rates of the two groups were compared. …”
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Human papillomavirus infection in women undergoing in-vitro fertilization: effects on embryo development kinetics and live birth rate by Federica Zullo, Valentina Fiano, Anna Gillio-Tos, Sara Leoncini, Ginevra Nesi, Luigia Macrì, Mario Preti, Alessandro Rolfo, Chiara Benedetto, Alberto Revelli, Laura De Marco

“…In the following days, embryo kinetic was comparable in the two groups until the early blastocyst stage, when embryos of HPV-positive women became significantly slower than those of HPV-negative women. …”
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Ultrasound Molecular Imaging of Neovascularization for Evaluation of Endometrial Receptivity Using Magnetic iRGD-Modified Lipid-Polymer Hybrid Microbubbles by He Y, Zhou M, Li S, Gong Z, Yan F, Liu H

“…Yanni He,1,* Meijun Zhou,1,* Sushu Li,1,* Zheli Gong,2 Fei Yan,3 Hongmei Liu1 1Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People’s Republic of China; 2Department of Ultrasound, The People’s Hospital of Hunan Province, Changsha, 410061, People’s Republic of China; 3CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongmei Liu, Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People’s Republic of China, Tel/Fax +86-19924270557, Email hongmeiliu3@163.com Fei Yan, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People’s Republic of China, Tel/Fax +86-755-86392284, Email fei.yan@siat.ac.cnBackground: Angiogenesis plays an important role in endometrial receptivity, determining the response of the endometrium to the blastocyst at the early stage of embryo implantation. …”
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Decreased HAT1 expression in granulosa cells disturbs oocyte meiosis during mouse ovarian aging by Bichun Guo, Sainan Zhang, Shanshan Wang, Huidan Zhang, Junshun Fang, Nannan Kang, Xin Zhen, Yang Zhang, Jidong Zhou, Guijun Yan, Haixiang Sun, Lijun Ding, Chuanming Liu

“…To explore the function of HAT1 in the process of mouse oocyte maturation, we used Anacardic Acid (AA) and small interfering RNAs (siRNA) to culture cumulus-oocyte complexes (COCs) from ICR female mice in vitro and gathered statistics of germinal vesicle breakdown (GVBD), the first polar body extrusion (PBE), meiotic defects, aneuploidy, 2-cell embryos formation, and blastocyst formation rate. Moreover, the human granulosa cell (GC)-like line KGN cells were used to investigate the mechanisms of HAT1 in this progress. …”
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The likelihood of a healthy live birth after frozen embryo transfer with endometrium prepared by natural ovulation regimen vs programmed regimen: a propensity-score matching studyA... by Ze Wang, MD, Yingxin Zhang, PhD, Xue Shang, MS, Ruolan Miao, MS, Mengfei Yin, MS, Huiming Yang, MS, Yunhai Yu, MD, Daimin Wei, PhD, MD

“…STUDY DESIGN: We conducted a retrospective cohort study including 7824 ovulatory women who underwent the first frozen embryo transfer cycle of single-blastocyst transfer with endometrial preparation by natural ovulation regimen vs programmed regimen, between June 2017 and June 2021. …”
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Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells by Lucie Gavin-Plagne, Florence Perold, Pierre Osteil, Sophie Voisin, Synara Cristina Moreira, Quitterie Combourieu, Véronique Saïdou, Magali Mure, Gérard Louis, Anne Baudot, Samuel Buff, Thierry Joly, Marielle Afanassieff

“…The resulting cells possess the same differentiation properties as blastocyst-stage embryonic stem cells (ESCs) and can be used to produce new individuals by embryonic complementation, nuclear transfer cloning, or in vitro fertilization after differentiation into male or female gametes. …”
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Deficiency of AP1 Complex <i>Ap1g1</i> in Zebrafish Model Led to Perturbation of Neurodevelopment, Female and Male Fertility; New Insight to Understand Adaptinopathies by Luca Mignani, Nicola Facchinello, Marco Varinelli, Elena Massardi, Natascia Tiso, Cosetta Ravelli, Stefania Mitola, Peter Schu, Eugenio Monti, Dario Finazzi, Giuseppe Borsani, Daniela Zizioli

“…<i>Ap1g1</i> (γ1) knockout mice cease development at the blastocyst stage, while <i>Ap1m1</i> (μ1A) knockouts cease during mid-organogenesis. …”
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Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep by Yukun Song, Hao Wu, Xuguang Wang, Aerman Haire, Xiaosheng Zhang, Jinlong Zhang, Yingjie Wu, Zhengxing Lian, Juncai Fu, Guoshi Liu, Abulizi Wusiman

“…In addition, 28 donors and 44 recipient ewes were used to produce morula/blastocyst and embryo transferring. Melatonin (5 mg) was given during estrus. …”
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Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes by Jing-Tao Sun, Jin-Dong Yuan, Qi Zhang, Xin Luo, Xin-Yue Qi, Jia-Hui Liu, Xi-Qing Jiang, Sanghoon Lee, Anukul Taweechaipaisankul, Zhong-Hua Liu, Jun-Xue Jin

“…Although there was no significant difference in cleavage rate, the blastocyst formation rate, total cell numbers, and hatching/-ed rate were significantly improved in 10⁻¹¹ M ramelteon group compared to the control (<i>p</i> < 0.05). …”
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Oocyte degeneration in a cohort adversely affects clinical outcomes in conventional IVF cycles: a propensity score matching study by Lanlan Liu, Lanlan Liu, Xiaoming Jiang, Zhenfang Liu, Jinghua Chen, Chao Yang, Kaijie Chen, Xiaolian Yang, Jiali Cai, Jiali Cai, Jianzhi Ren

“…The PS matching method was used to control for potential confounding factors, and a multivariate logistic regression analysis was made to evaluate whether the presence of oocyte degeneration would affect the cumulative live birth rate (CLBR).ResultsAfter PS matching, basic characteristics were similar between the two groups, oocyte yield was significantly higher in the DEG group than the NON-DEG group (P &lt; 0.05), mature oocyte number, 2 pronuclear (2PN) embryo number, 2PN embryo clearage rate, “slow” embryo number, “accelerated” embryo number, rate of cycles with total day 3 embryo extended culture, number of frozen embryo transfer (FET) cycles, transferred embryo stage, transferred embryo number, and live birth rate in fresh embryo transfer cycles were all similar between the two groups (P &gt; 0.05), but the 2PN fertilization rate, available embryo number, high-quality embryo number, “normal” embryo number, frozen embryo number, blastocyst formation rate, and no available embryo cycle rate were all significantly lower in the DEG group than the NON-DEG group (P &lt; 0.05). …”
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The effects of laser-assisted zona pellucida manipulations on embryo competency and the mechanisms underlying embryo hatching in a mouse model by Liu, Y

“…No overt deleterious effects were identified with regards to the blastocyst development, cell apoptosis, heat shock stress (HSP70), and the expression of key developmental genes. …”

Exogenous L-Glutathione Improves Vitrification Outcomes in Murine Preimplantation Embryos by Nor-Shahida Abdul Rahman, Nor-Ashikin Mohamed Noor Khan, Zolkapli Eshak, Mimi-Sophia Sarbandi, Aqila-Akmal Mohammad Kamal, Mastura Abd Malek, Fathiah Abdullah, Maizaton Atmadini Abdullah, Fezah Othman

“…In the non-vitrified groups, there were significant increases in the number of Grade-1 blastocysts in GSH cultures (<i>p</i> < 0.05). Similarly, in the vitrified groups, GSH supplementation was also seen to significantly increase blastocyst formation. …”
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Blastomere aggregation using phytohemagglutinin-L improves the establishment efficiency of porcine parthenogenesis-derived embryonic stem-like cell lines by Joohyeong Lee, Joohyeong Lee, Lian Cai, Lian Cai, Lian Cai, Mirae Kim, Mirae Kim, Hyerin Choi, Hyerin Choi, Dongjin Oh, Dongjin Oh, Ali Jawad, Ali Jawad, Eunsong Lee, Sang-Hwan Hyun, Sang-Hwan Hyun, Sang-Hwan Hyun

“…Aggregation of blastomeres is a promising method to improve the developmental competence of blastocysts and may be useful for the production of chimeric animals and the establishment of embryonic stem cell lines by increasing inner cell masses. …”
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WNT Co-Receptor LRP6 Is Critical for Zygotic Genome Activation and Embryonic Developmental Potential by Interacting with Oviductal Paracrine Ligand WNT2 by Fusheng Yao, Jia Hao, Zhaochen Wang, Meiqiang Chu, Jingyu Zhang, Guangyin Xi, Zhenni Zhang, Lei An, Jianhui Tian

“…More importantly, we found that WNT2 supplementation in culture medium significantly promoted zygotic genome activation (ZGA) and improved blastocyst formation and quality following in vitro fertilization (IVF). …”
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Can biomarkers identified from the uterine fluid transcriptome be used to establish a noninvasive endometrial receptivity prediction tool? A proof-of-concept study by Aihua He, Hong Wu, Yangyun Zou, Cheng Wan, Jing Zhao, Qiong Zhang, Nenghui Liu, Donge Liu, Yumei Li, Jing Fu, Hui Li, Xi Huang, Tianli Yang, Chunxu Hu, Zhaojuan Hou, Yue Sun, Xin Dong, Jian Wu, Sijia Lu, Yanping Li

“…Subsequently, 22 IVF patients who underwent frozen-thaw blastocyst transfer were recruited and analyzed the correlation between the predicted results of nirsERT and pregnancy outcomes. …”
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Ectopic Pregnancy: An Overview by Daniele Di Gennaro, Gianluca Raffaello Damiani, Giuseppe Muzzupapa, Massimo Stomati, Rossana Cicinelli, Maria Gaetani, Luca Maria Schonauer, Edoardo Di Naro, Giuseppe Trojano, Ettore Cicinelli

“…EP is a pathological condition characterized by an abnormal blastocyst implant at any site apart from uterine cavity. …”
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Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods by Quynh Anh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Lanh Thi Kim Do, Takeshige Otoi

“…Conclusion: The combination of CRISPR/Cas9 delivery methods may improve the mutation efficiency of triple-gene edited porcine blastocysts.…”
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Copper deficiency affects the developmental competence of porcine oocytes matured in vitro by Hyerin Choi, Hyerin Choi, Dongjin Oh, Dongjin Oh, Mirae Kim, Mirae Kim, Lian Cai, Lian Cai, Lian Cai, Joohyeong Lee, Joohyeong Lee, Eunhye Kim, Gabsang Lee, Sang-Hwan Hyun, Sang-Hwan Hyun, Sang-Hwan Hyun

“…TEPA-treated oocytes showed significantly decreased blastocyst formation rates compared to those of the control. …”
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Analysis of predictors of clinical pregnancy and live birth in patients with RIF treated with IVF-ET technology: a cohort study based on a propensity score approach by Yan Jia, Yan Jia, Yan Jia, Zhonghua Ai, Xianglong Zhu, Zhuohang Che, Adhikari Pratikshya, Songyuan Tang, Qiong Zhang

“…Patients were recruited who were enrolled at this hospital between November 1, 2019 and August 31, 2022, and who met the following criteria: a frozen embryo transfer (FET) at day 5 or 6 blastocyst stage was performed and the number of transfer cycles was not less than two. …”
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Derivation of trophoblast stem cells from naïve human pluripotent stem cells by Chen Dong, Mariana Beltcheva, Paul Gontarz, Bo Zhang, Pooja Popli, Laura A Fischer, Shafqat A Khan, Kyoung-mi Park, Eun-Ja Yoon, Xiaoyun Xing, Ramakrishna Kommagani, Ting Wang, Lilianna Solnica-Krezel, Thorold W Theunissen

“…Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs are similar to blastocyst-derived hTSCs and acquire features of post-implantation trophectoderm. …”
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