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1481
Artificial oocyte activation with Ca2+ ionophore improves reproductive outcomes in patients with fertilization failure and poor embryo development in previous ICSI cycles
Published 2023-08-01“…The fertilization rates, cleavage rates, transferable embryo rates and blastocyst formation rates of the two groups were compared. …”
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1482
Human papillomavirus infection in women undergoing in-vitro fertilization: effects on embryo development kinetics and live birth rate
Published 2023-04-01“…In the following days, embryo kinetic was comparable in the two groups until the early blastocyst stage, when embryos of HPV-positive women became significantly slower than those of HPV-negative women. …”
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1483
Ultrasound Molecular Imaging of Neovascularization for Evaluation of Endometrial Receptivity Using Magnetic iRGD-Modified Lipid-Polymer Hybrid Microbubbles
Published 2022-12-01“…Yanni He,1,* Meijun Zhou,1,* Sushu Li,1,* Zheli Gong,2 Fei Yan,3 Hongmei Liu1 1Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People’s Republic of China; 2Department of Ultrasound, The People’s Hospital of Hunan Province, Changsha, 410061, People’s Republic of China; 3CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongmei Liu, Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, Guangzhou, 510317, People’s Republic of China, Tel/Fax +86-19924270557, Email hongmeiliu3@163.com Fei Yan, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, People’s Republic of China, Tel/Fax +86-755-86392284, Email fei.yan@siat.ac.cnBackground: Angiogenesis plays an important role in endometrial receptivity, determining the response of the endometrium to the blastocyst at the early stage of embryo implantation. …”
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1484
Decreased HAT1 expression in granulosa cells disturbs oocyte meiosis during mouse ovarian aging
Published 2023-10-01“…To explore the function of HAT1 in the process of mouse oocyte maturation, we used Anacardic Acid (AA) and small interfering RNAs (siRNA) to culture cumulus-oocyte complexes (COCs) from ICR female mice in vitro and gathered statistics of germinal vesicle breakdown (GVBD), the first polar body extrusion (PBE), meiotic defects, aneuploidy, 2-cell embryos formation, and blastocyst formation rate. Moreover, the human granulosa cell (GC)-like line KGN cells were used to investigate the mechanisms of HAT1 in this progress. …”
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1485
The likelihood of a healthy live birth after frozen embryo transfer with endometrium prepared by natural ovulation regimen vs programmed regimen: a propensity-score matching studyA...
Published 2023-05-01“…STUDY DESIGN: We conducted a retrospective cohort study including 7824 ovulatory women who underwent the first frozen embryo transfer cycle of single-blastocyst transfer with endometrial preparation by natural ovulation regimen vs programmed regimen, between June 2017 and June 2021. …”
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1486
Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells
Published 2020-10-01“…The resulting cells possess the same differentiation properties as blastocyst-stage embryonic stem cells (ESCs) and can be used to produce new individuals by embryonic complementation, nuclear transfer cloning, or in vitro fertilization after differentiation into male or female gametes. …”
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1487
Deficiency of AP1 Complex <i>Ap1g1</i> in Zebrafish Model Led to Perturbation of Neurodevelopment, Female and Male Fertility; New Insight to Understand Adaptinopathies
Published 2023-04-01“…<i>Ap1g1</i> (γ1) knockout mice cease development at the blastocyst stage, while <i>Ap1m1</i> (μ1A) knockouts cease during mid-organogenesis. …”
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1488
Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep
Published 2019-04-01“…In addition, 28 donors and 44 recipient ewes were used to produce morula/blastocyst and embryo transferring. Melatonin (5 mg) was given during estrus. …”
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1489
Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes
Published 2022-08-01“…Although there was no significant difference in cleavage rate, the blastocyst formation rate, total cell numbers, and hatching/-ed rate were significantly improved in 10<sup>−11</sup> M ramelteon group compared to the control (<i>p</i> < 0.05). …”
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1490
Oocyte degeneration in a cohort adversely affects clinical outcomes in conventional IVF cycles: a propensity score matching study
Published 2023-05-01“…The PS matching method was used to control for potential confounding factors, and a multivariate logistic regression analysis was made to evaluate whether the presence of oocyte degeneration would affect the cumulative live birth rate (CLBR).ResultsAfter PS matching, basic characteristics were similar between the two groups, oocyte yield was significantly higher in the DEG group than the NON-DEG group (P < 0.05), mature oocyte number, 2 pronuclear (2PN) embryo number, 2PN embryo clearage rate, “slow” embryo number, “accelerated” embryo number, rate of cycles with total day 3 embryo extended culture, number of frozen embryo transfer (FET) cycles, transferred embryo stage, transferred embryo number, and live birth rate in fresh embryo transfer cycles were all similar between the two groups (P > 0.05), but the 2PN fertilization rate, available embryo number, high-quality embryo number, “normal” embryo number, frozen embryo number, blastocyst formation rate, and no available embryo cycle rate were all significantly lower in the DEG group than the NON-DEG group (P < 0.05). …”
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1491
The effects of laser-assisted zona pellucida manipulations on embryo competency and the mechanisms underlying embryo hatching in a mouse model
Published 2021“…No overt deleterious effects were identified with regards to the blastocyst development, cell apoptosis, heat shock stress (HSP70), and the expression of key developmental genes. …”
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1492
Exogenous L-Glutathione Improves Vitrification Outcomes in Murine Preimplantation Embryos
Published 2022-10-01“…In the non-vitrified groups, there were significant increases in the number of Grade-1 blastocysts in GSH cultures (<i>p</i> < 0.05). Similarly, in the vitrified groups, GSH supplementation was also seen to significantly increase blastocyst formation. …”
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1493
Blastomere aggregation using phytohemagglutinin-L improves the establishment efficiency of porcine parthenogenesis-derived embryonic stem-like cell lines
Published 2022-09-01“…Aggregation of blastomeres is a promising method to improve the developmental competence of blastocysts and may be useful for the production of chimeric animals and the establishment of embryonic stem cell lines by increasing inner cell masses. …”
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1494
WNT Co-Receptor LRP6 Is Critical for Zygotic Genome Activation and Embryonic Developmental Potential by Interacting with Oviductal Paracrine Ligand WNT2
Published 2023-04-01“…More importantly, we found that WNT2 supplementation in culture medium significantly promoted zygotic genome activation (ZGA) and improved blastocyst formation and quality following in vitro fertilization (IVF). …”
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1495
Can biomarkers identified from the uterine fluid transcriptome be used to establish a noninvasive endometrial receptivity prediction tool? A proof-of-concept study
Published 2023-02-01“…Subsequently, 22 IVF patients who underwent frozen-thaw blastocyst transfer were recruited and analyzed the correlation between the predicted results of nirsERT and pregnancy outcomes. …”
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1496
Ectopic Pregnancy: An Overview
Published 2022-11-01“…EP is a pathological condition characterized by an abnormal blastocyst implant at any site apart from uterine cavity. …”
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1497
Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
Published 2022-09-01“…Conclusion: The combination of CRISPR/Cas9 delivery methods may improve the mutation efficiency of triple-gene edited porcine blastocysts.…”
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1498
Copper deficiency affects the developmental competence of porcine oocytes matured in vitro
Published 2022-09-01“…TEPA-treated oocytes showed significantly decreased blastocyst formation rates compared to those of the control. …”
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1499
Analysis of predictors of clinical pregnancy and live birth in patients with RIF treated with IVF-ET technology: a cohort study based on a propensity score approach
Published 2024-04-01“…Patients were recruited who were enrolled at this hospital between November 1, 2019 and August 31, 2022, and who met the following criteria: a frozen embryo transfer (FET) at day 5 or 6 blastocyst stage was performed and the number of transfer cycles was not less than two. …”
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1500
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
Published 2020-02-01“…Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs are similar to blastocyst-derived hTSCs and acquire features of post-implantation trophectoderm. …”
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