Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer by Khine Myint, Linda Shyue Huey Chuang, Yu Xuan Teh, Nur Astiana Mawan, Edward Jizhong Shi, Michelle Meng Huang Mok, Napat Nuttonmanit, Junichi Matsuo, Ying Li, Henry Yang, Atsushi Okabe, Atsushi Kaneda, Motomi Osato, Jimmy Bok-Yan So, Wei Peng Yong, Patrick Tan, Khay Guan Yeoh, Yoshiaki Ito

“…Here, we show that IGF2BP1 and IQGAP3 expression levels are highest in the blastocyst, with both decreasing during adulthood. This suggests that IQGAP3, like IGF2BP1, is an early developmental gene that is aberrantly upregulated upon re-expression of IGF2BP1 during carcinogenesis. …”
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Extracellular Vesicles Secreted by Pre-Hatching Bovine Embryos Produced In Vitro and In Vivo Alter the Expression of IFNtau-Stimulated Genes in Bovine Endometrial Cells by Constanza Aguilera, Alejandra Estela Velásquez, Miguel Angel Gutierrez-Reinoso, Yat Sen Wong, Barbara Melo-Baez, Joel Cabezas, Diego Caamaño, Felipe Navarrete, Daniela Rojas, Gonzalo Riadi, Fidel Ovidio Castro, Llretny Rodriguez-Alvarez

“…In bovines, the secretion of interferon Tau (IFNT) during elongation is the main signal for pregnancy recognition, but its expression starts around the blastocyst stage. Embryos release extracellular vesicles (EVs) as an alternative mechanism of embryo-maternal communication. …”
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An Optogenetic‐Controlled Cell Reprogramming System for Driving Cell Fate and Light‐Responsive Chimeric Mice by Meiyan Wang, Yuanxiao Liu, Ziwei Wang, Longliang Qiao, Xiaoding Ma, Lingfeng Hu, Deqiang Kong, Yuan Wang, Haifeng Ye

“…This system enables remote, non‐invasive optogenetical regulation of endogenous Sox2 and Oct4 loci to reprogram mouse embryonic fibroblasts into iPSCs (iPSCLIRE) under light‐emitting diode‐based illumination. iPSCLIRE cells can be efficiently differentiated into different cells by upregulating a corresponding TF. iPSCLIRE cells are used for blastocyst injection and optogenetic chimeric mice are successfully generated, which enables non‐invasive control of user‐defined endogenous genes in vivo, providing a valuable tool for facile and traceless controlled gene expression studies and genetic screens in mice. …”
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Impact of COVID-19 convalescence on pregnancy outcomes in patients undergoing IVF/ICSI during fresh ART cycles: a retrospective cohort study by Mingya Cao, Yan Han, Tengfei Feng, Peiyang Lu, Yue Wang, Qingyun Sun, Zhiming Zhao, Wensen Pan

“…Although the number of blastocysts formed was lower in the COVID-19 group (P=0.017), this group also exhibited a higher blastocyst freezing rate and a higher rate of high-quality embryos per retrieved oocyte (P<0.001 and P=0.023, respectively). …”
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Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes by Desmond A. R. Tutt, Desmond A. R. Tutt, Gizem Guven-Ates, Gizem Guven-Ates, Wing Yee Kwong, Wing Yee Kwong, Rob Simmons, Fei Sang, Giuseppe Silvestri, Carla Canedo-Ribeiro, Alan H. Handyside, Remi Labrecque, Marc-André Sirard, Richard D. Emes, Richard D. Emes, Darren K. Griffin, Kevin D. Sinclair, Kevin D. Sinclair

“…These effects preceded the establishment of distinct metabolic and epigenetic signatures several days later in expanded and hatching blastocysts.ConclusionsThese findings highlight the importance of lipid, particularly sterol, metabolism by the COC during IVM. …”
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Identification of zona pellucida defects revealed a novel loss-of-function mutation in ZP2 in humans and rats by Jun Zeng, Ying Sun, Jing Zhang, Xiaozhu Wu, Yan Wang, Ruping Quan, Wanjuan Song, Dan Guo, Shengran Wang, Jianlin Chen, Hongmei Xiao, Hongmei Xiao, Hua-Lin Huang

“…The patient successfully conceived by receiving the only two embryos that developed to the blastocyst stage. The immunofluorescence staining showed an apparently abnormal form of the stopped oocytes. …”
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Intra-ovarian inflammatory states and their associations with embryo quality in normal-BMI PCOS patients undergoing IVF treatment by Jie Shang, Siyu Wang, Aiyuan Wang, Fang Li, Jing Zhang, Jin Wang, Rui Lv, Haixia Chen, Xiaohuan Mu, Kai Zhang, Xiaohong Bai, Ye Tian

“…Besides, FF MIP-1β showed an inverse correlation with the number of D3 good-quality embryos and the good-quality blastocyst rate in patients with PCOS (P = 0.006; P = 0.003, respectively), which remained significant after correction for multiple comparisons. …”
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hsa-miR-320a-3p and hsa-miR-483-5p levels in human granulosa cells: promising bio-markers of live birth after IVF/ICSI by Yu Liu, Qiaojuan Mei, Jiahao Yang, Qiuzi Shen, Min Zou, Jiao Li, Huaibiao Li, Ling Zhang, Wenpei Xiang

“…Patients in Q3 were less likely to achieve a normal fertilization rate for in vitro fertilization and blastocyst formation than those in Q1 as they expressed high levels of hsa-miR-320a-3p and hsa-miR-483-5p (P < 0.05). …”
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The influence of embryo stage on obstetric complications and perinatal outcomes following programmed compared to natural frozen-thawed embryo transfer cycles: a systematic review a... by Zhonghua Zhao, Zhonghua Zhao, Zhonghua Zhao, Zhonghua Zhao, Yan Chen, Yan Chen, Yan Chen, Yan Chen, Hongxia Deng, Hongxia Deng, Hongxia Deng, Hongxia Deng, Lu Huang, Lu Huang, Lu Huang, Lu Huang, Danhua Lu, Danhua Lu, Danhua Lu, Danhua Lu, Xiaoyang Shen, Xiaoyang Shen, Xiaoyang Shen, Xiaoyang Shen, Liangzhi Xu, Liangzhi Xu, Liangzhi Xu, Liangzhi Xu

“…Cleavage-stage embryo transfers revealed no difference in HDPs (1 study, OR 0.81; 95% CI 0.32-2.02; P=0.65; I2 not applicable), gestational hypertension (2 studies, OR 0.85; 95% CI 0.48-1.51; P=0.59; I2 = 0%), PE (1 study, OR 1.19; 95% CI 0.58-2.42; P=0.64; I2not applicable), GDM (3 study, OR 0.79; 95% CI 0.52-1.20; P=0.27; I2 = 21%), LGA (1 study, OR 1.15; 95% CI 0.62-2.11; P=0.66; I2not applicable), macrosomia (1 study, OR 1.22; 95% CI 0.54-2.77; P=0.64; I2 not applicable), PTD (2 studies, OR 1.05; 95% CI 0.74-1.49; P=0.79; I2 = 0%), PPH (1 study, OR 1.49; 95% CI 0.85-2.62; P=0.17; I2not applicable), or PPROM (2 studies, OR 0.74; 95% CI 0.46-1.21; P=0.23; I2 = 0%) between programmed FET cycles and natural FET cycles.Conclusion(s)The risks of HDPs, gestational hypertension, PE, GDM, LGA, macrosomia, SGA, PTD, placenta previa, PPROM, and PPH were increased in programmed FET cycles versus natural FET cycles with overall embryo transfer and blastocyst transfer, but the risks were not clear for cleavage-stage embryo transfer.…”
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One-Step In Vitro Generation of ETV2-Null Pig Embryos by Marta Moya-Jódar, Giulia Coppiello, Juan Roberto Rodríguez-Madoz, Gloria Abizanda, Paula Barlabé, Amaia Vilas-Zornoza, Asier Ullate-Agote, Chiara Luongo, Ernesto Rodríguez-Tobón, Sergio Navarro-Serna, Evelyne París-Oller, Maria Oficialdegui, Xonia Carvajal-Vergara, Laura Ordovás, Felipe Prósper, Francisco Alberto García-Vázquez, Xabier L. Aranguren

“…To meet this clinical demand, one of the last frontiers of regenerative medicine is the generation of humanized organs in pigs from pluripotent stem cells (PSCs) via blastocyst complementation. For this, organ-disabled pig models are needed. …”
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Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy by Yufei Jiang, Yan He, Songting Liu, Gaizhen Li, Dunjin Chen, Wenbo Deng, Ping Li, Ying Zhang, Jinxiang Wu, Jianing Li, Longmei Wang, Jiajing Lin, Haibin Wang, Shuangbo Kong, Guixiu Shi

“…Finally, we disclosed that over-active inflammatory state in Gαq-deficient decidua deferred the blastocyst hatching and adhesion in vitro, and the decidual expression of Gαq was significantly lower in women with recurrent pregnancy loss compared with normal pregnancy. …”
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Effect of noninvasive embryo viability testing versus conventional IVF on the live birth rate in IVF/ICSI patients: a study protocol for a double-blind, multicenter, randomized con... by Yan-Fei Cheng, Cui-Lian Zhang, Yun Liu, Jian-Ping Ou, Lei Chen, Gui-Feng Cai, Zu Yang, Tian-Min Ye, Jun Wang, Juan-Ke Xie, Ping Xiong, Xi-Ya Zhang, Min Li, Wei-Biao Xu, Xiao-Qing Wang, Ling-Yin Kong, Bo Liang, Xiao-Hong Wang, Yue-Qiang Wang, Yuan-Qing Yao

“…As an alternative, noninvasive PGT-A (niPGT-A) approaches using spent blastocyst culture medium (SBCM) achieved comparable accuracy with PGT-A in several pilot studies. …”
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Dietary flaxseed oil and vitamin E improve semen quality via propionic acid metabolism by Chongshan Yuan, Chongshan Yuan, Kaiyan Zhang, Kaiyan Zhang, Zhe Wang, Zhe Wang, Xin Ma, Xin Ma, Hongyu Liu, Hongyu Liu, Jing Zhao, Jing Zhao, Wenfa Lu, Wenfa Lu, Jun Wang, Jun Wang

“…Compared with the control group, sperm from the FOVE group had a significantly higher in vitro fertilization (IVF) rate, and subsequent embryos had increased developmental ability with reduced ROS levels at the eight-cell stage, then increased adenosine triphosphate (ATP) content and gene expression levels of CAT, CDX2, Nanog, and SOD at the blastocyst stage (P &lt; 0.05). Metabolomic and transcriptomic results indicated that dietary supplementation of FO and VE increased the expression of the metabolite aconitic acid, as well as the expression of ABAT and AHDHA genes.ConclusionWith in-silico analysis, it can be concluded that the effects of dietary FO and VE on improving semen quality and embryo development may be related to increased aconitic acid via the ABAT and AHDHA genes involved in the propionic acid metabolism pathway.…”
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Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness by Zili Gao, Jia Guo, Bo Gou, Zhen Gu, Tan Jia, Sinan Ma, Liyuan Jiang, Wenli Liu, Lixun Zhou, Qi Gu

“…Compared with the spheroid, formed by the self-assembly of mTSCs (T(sph)), we could regulate the stiffness of the microcarrier, making the Young's modulus of mTSCs (367.70 ± 79.81 Pa) similar to that of the blastocyst trophoblast ectoderm (432.49 ± 151.90 Pa). …”
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Pregnancy incidence and associated risk factors of dichorionic triamniotic triplet under assisted reproduction: A large sample of clinical data analysis by Shuhua Liu, Shuhua Liu, Qianhua Xu, Qianhua Xu, Qianhua Xu, Qianhua Xu, Yafen Wang, Bing Song, Bing Song, Bing Song, Bing Song, Zhaolian Wei, Zhaolian Wei, Zhaolian Wei, Zhaolian Wei

“…There were significant differences in DCTA triplet pregnancies rate among receiving intracytoplasmic sperm injection (ICSI) and receiving in vitro fertilization (IVF) [1.92% vs. 1.02%, p &lt; 0.001, OR = 0.461, 95% confidence interval (CI) 0.315–0.673], blastocyst transfer (BT) versus cleavage-ET (1.66% vs. 0.57%, P &lt; 0.001, OR = 0.329, 95% CI 0.315–0.673), and maternal age ≥ 35 years versus maternal age &lt; 35 years (1.00% vs. 1.30%, P = 0.040, OR = 1.773, 95% CI 1.025–3.066). …”
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Superior performance of biocomposite nanoparticles PLGA-RES in protecting oocytes against vitrification stimuli by Guiping Hai, Jiachen Bai, Yucheng Liu, Jun Li, Aiju Liu, Jingjing Wang, Qian Liu, Weijun Liu, Pengcheng Wan, Xiangwei Fu

“…Notably, PLGA-RES even increased maturation (65.10% ± 4.11% vs. 52.85% ± 2.87%, p &lt; 0.05) and blastocyst rate (56.13% ± 1.36% vs. 40.91% ± 5.85%, p &lt; 0.05). …”
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Relationship of field and in vitro fertility of dairy bulls with sperm parameters, including DAG1 and SERPINA5 proteins by Saulo Menegatti Zoca, Saulo Menegatti Zoca, Julie A. Walker, Adalaide C. Kline, Taylor N. Andrews, Jerica J. J. Rich, Kaitlin M. Epperson, Kaitlin M. Epperson, Jessica Nora Drum, Jessica Nora Drum, M. Sofia Ortega, M. Sofia Ortega, Robert A. Cushman, George A. Perry

“…Thus, the objectives of this study were to characterize the localization and abundance variability of DAG1 and SERPINA5 in bovine sperm, and to investigate the relationship of DAG1 and SERPINA5 with field fertility (i.e., sire conception rate; SCR), in vitro embryo production (IVP), and sperm parameters.Material and methodsDairy bulls (n = 22) were classified as high-SCR (SCR &gt; 1.0) or low-SCR (SCR &lt; –4.0), and good [blastocyst (BL)-by-cleavage (CL) ratio (BL/CL) &gt; 39%] or poor (BL/CL &lt; 38%) BL/CL. …”
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Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk by Ellen Menkhorst, Ellen Menkhorst, Teresa So, Teresa So, Kate Rainczuk, Siena Barton, Siena Barton, Wei Zhou, Wei Zhou, Tracey Edgell, Evdokia Dimitriadis, Evdokia Dimitriadis, Evdokia Dimitriadis

“…IntroductionA healthy pregnancy requires successful blastocyst implantation into an adequately prepared or ‘receptive’ endometrium. …”
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The Impact of Malignancy on Assisted Reproductive Outcomes for Cancer Survivors: A Retrospective Case–Control Study by Yuehong Li, Xuefen Cai, Binhua Dong, Binhua Dong, Qi Wang, Xiaohui Yang, Aili Yu, Huijuan Wei, Zhanghong Ke, Pengming Sun, Pengming Sun, Beihong Zheng, Yan Sun

“…The total gonadotropin dose, number of oocytes retrieved, fertilization rate, cleavage rate, high-quality embryo rate, blastocyst rate and first-time embryo-transfer (ET) implantation rate were nonsignificantly lower in the cancer group than in the control group (P&gt;0.05). …”
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Predictive models of recurrent implantation failure in patients receiving ART treatment based on clinical features and routine laboratory data by Qunying Fang, Zonghui Qiao, Lei Luo, Shun Bai, Min Chen, Xiangjun Zhang, Lu Zong, Xian-hong Tong, Li-min Wu

“…The area under the curve of the logistic regression model was 0.900 (95% CI, 0.870–0.929) for the training cohort and 0.895 (95% CI, 0.865–0.925) for the testing cohort. (2) Advanced age (1.069; 95% CI, 1.015–1.126) was a risk factor associated with no live births after the first cycle of subsequent assisted reproduction treatment in patients with RIF. Blastocyst transfer (0.365; 95% CI = 0.181–0.736) increased the probability of live birth in subsequent cycles in patients with RIF. …”
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