Evidence of Selection Against Damaged Mitochondria During Early Embryogenesis in the Mouse by Thiago S. Machado, Thiago S. Machado, Carolina H. Macabelli, Maite Del Collado, Flávio V. Meirelles, Flávio V. Meirelles, Francisco E. G. Guimarães, Marcos R. Chiaratti, Marcos R. Chiaratti

“…Given that total mtDNA content remained stable between stages (zygotes vs. blastocysts) and treatments (photoirradiated vs. non-photoirradiated), these results indicate that the photoirradiated-derived mtDNA was replaced by recipient mtDNA in blastocysts. …”
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Assessment of the effectiveness of methods for the selection of sperm with increased DNA fragmentation in ART cycles by Leontyeva Eugene, Kim Alena, Zastavskiy Ivan, Yakhyarova Mukhabbat, Polumiskov Vadim

“…The formation of blastocysts of any quality did not exhibit any notable variations (p=0.4139; p>0.999; p>0.999). …”
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TET family regulates the embryonic pluripotency of porcine preimplantation embryos by maintaining the DNA methylation level of NANOG by Kyungjun Uh, Junghyun Ryu, Kayla Farrell, Noah Wax, Kiho Lee

“…Surprisingly, a threefold increase in the transcript level of TET3 was observed in blastocysts carrying modified TET1, which may explain the upregulation of NANOG and ESRRB. …”
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Development, Quality, and Production Parameters of In Vitro Embryo in Anatolian Water Buffaloes by M. A. Yılmaz, M. Kaymaz, Y. Ergün

“…The number of cleavages at the 24th hour was 93 out of 302 (30.79%), the number of morulae and compact morula at the 96th hour was 53 out of 302 (17.55%), the number of blastocysts at 7th day was 29 out of 302 (9.60%), and the number of hatched blastocysts were 12 out of 302 (3.97%) on the 8th and 9th days. …”
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Initiation of X Chromosome Inactivation during Bovine Embryo Development by Bo Yu, Helena T. A. van Tol, Tom A.E. Stout, Bernard A. J. Roelen

“…In female mouse embryos, paternal XCI is initiated at the 4-cell stage; however, the X chromosome is reactivated in the inner cell mass cells of blastocysts, and random XCI is subsequently initiated in epiblast cells. …”
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Generation of Rhesus Macaque Embryos with Expanded CAG Trinucleotide Repeats in the Huntingtin Gene by Ryu, Junghyun, Statz, John P., Chan, William, Oyama, Kiana, Custer, Maggie, Wienisch, Martin, Chen, Richard, Hanna, Carol B., Hennebold, Jon D.

“…Analyses were conducted on arrested embryos and trophectoderm (TE) cells biopsied from blastocysts to assess the insertion of the ssDNA into the <i>HTT</i> gene. …”
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Heat Shock Protein 70 Improves In Vitro Embryo Yield and Quality from Heat Stressed Bovine Oocytes by Konstantina Stamperna, Themistoklis Giannoulis, Eleni Dovolou, Maria Kalemkeridou, Ioannis Nanas, Katerina Dadouli, Katerina Moutou, Zissis Mamuris, Georgios S. Amiridis

“…Gene expression was assessed by RT-PCR in oocytes, cumulus cells and blastocysts. In C41, blastocysts formation rate was lower than in C39 and on day 9 it was lower than in H41. …”
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Rabbit as an Aging Model in Reproduction: Advanced Maternal Age Alters GLO1 Expression in the Endometrium at the Time of Implantation by Johanna de Nivelle, Juliane Thoma, Alicia Toto Nienguesso, Tom Seeling, Juliane-Susanne Jung, Anne Navarrete Santos, Maria Schindler

“…At day 6 post coitum, uterine, oviductal, ovarian tissue and blastocysts from young (16–20 weeks) and old rabbits (>108 weeks) were characterised for GLO1 expression. …”
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Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells by Ramon Cesar Botigelli, Ramon Cesar Botigelli, Naira Carolina Godoy Pieri, Brendon William Bessi, Lucas Simões Machado, Lucas Simões Machado, Alessandra Bridi, Aline Fernanda de Souza, Kaiana Recchia, Paulo Fantinato Neto, Pablo Juan Ross, Fabiana Fernandes Bressan, Marcelo Fábio Gouveia Nogueira, Marcelo Fábio Gouveia Nogueira

“…Moreover, biPSCs and bESCs labeled with GFP were able to contribute to chimeric blastocysts. Additionally, biPSCs have shown promising potential for contributing to chimeric blastocysts and for future studies.…”
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Expression of RONIN and NANOG-associated proteins in goat parthenogenetic embryos by Marcelo Tigre Moura, Pamela Ramos-Deus, José Carlos Ferreira-Silva, Priscila Germany Corrêa Silva, Ludymila Furtado Cantanhêde, Pábola Santos Nascimento, Roberta Lane Oliveira Silva, Ana Maria Benko-Iseppon, Marcos Antonio Lemos Oliveira

“…Moreover, all five PATF were detected in blastocysts by RT-qPCR (ZFP281, NAC1, RONIN, OCT4, and NR0B1). …”
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Gamma‐aminobutyric acid (GABA) can affect physiological processes in preimplantation embryos via GABAA and GABAB receptors by Veronika Kovaříková, Alexandra Špirková, Zuzana Šefčíková, Jozef Pisko, Laura Kalatová, Juraj Koppel, Dušan Fabian, Štefan Čikoš

“…Results We detected nine GABA receptor transcripts in mouse blastocysts and 14 GABA receptor transcripts in ovulated oocytes. …”
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Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos by Norikazu MIYASHITA, Satoshi AKAGI, Tamas SOMFAI, Yuji HIRAO

“…Embryonic transfer of bovine blastocysts produced using in vitro fertilization (IVF) is widely used, although the challenge of compromised conception rates remains. …”
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Potential of preimplantation genomic selection for carcass traits in Japanese Black cattle by Takashi FUJII, Akira NAITO, Hiroki HIRAYAMA, Masashi KASHIMA, Hitomi YOSHINO, Takeshi HANAMURE, Yukio DOMON, Hiroyuki HAYAKAWA, Toshio WATANABE, Satoru MORIYASU, Soichi KAGEYAMA

“…In Experiment 2, a total of 208 in vivo blastocysts were biopsied (~15-cell) and the biopsied cells were processed for SNP genotyping, where 88.5% of the samples were found to be suitable for GEBV calculation. …”
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Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes by Shinsuke Seki, Toshiaki Kawabe, Wataru Yamazaki, Kazuaki Matsumura, Takanori Oikawa, Takahiro Obata, Misako Higashiya, Megumi Yano, Tomoo Eto

“…Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo following embryo transfer. …”
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Temporal and Spatial Regulation of miR-320 in the Uterus during Embryo Implantation in the Rat by Chun-Mei Liu, Yi Cui, Pei-Pei Song, Xiao-Hua Jin, Hong-Fei Xia, Xu Ma

“…The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. There were reports to show that some microRNAs (miRNAs) may play a key role during embryo implantation in mouse. …”
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Transcriptome profiling reveals superovulation with the gonadotropin-releasing hormone agonist trigger impaired embryo implantation in mice by Meng Li, Jingmei Han, Nana Yang, Xiangyun Li, Xinglong Wu

“…We compared the number of oocytes, blastocysts, and corpus luteum between the two groups and the implantation sites after the transfer of natural blastocysts. …”
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Tasa de fertilización, desarrollo y calidad de embriones bovinos Holstein producidos in vitro con semen sexado y adición de IGF-I by Octavio Martínez-Guerrero, Javier Antillón-Ruíz, Felipe Alonso Rodríguez-Almeida

“…The addition of IGF-I to the media affected the fertilization rate (34 vs 42%; P < 0.05) and there was not effect (P > 0.05) of treatment for the rate blastocysts/fertilized oocytes fertilized or for the quality of blastocysts. …”
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Effects of Hydrosalpinx on Endometrial Implantation Failures: Evaluating Salpingectomy in Women Undergoing in vitro fertilization by Antonio Palagiano, Mauro Cozzolino, Filippo Maria Ubaldi, Chiara Palagiano, Maria Elisabetta Coccia

“…The window of endometrial receptivity is essential in the implantation of blastocysts, and it triggers multiple reactions arising from the endometrium as well as the blastocysts. …”
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Freeze-all, for whom, when, and how by Paula Celada, Ernesto Bosch

“…Thus, it has been demonstrated that elevation of progesterone at the end of ovarian stimulation decreases the implantation rates after the transfer of day 6 blastocysts in fresh and some uterine pathologies; freeze-all is also the preferred option for patients undergoing pre-implantation genetic testing, since there is an improvement of the results and it allows for inclusion of all blastocysts of the cohort. …”
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LH level on the antagonist administration day as a predictor of the reproductive outcomes in women with normal ovarian function by Conghui Pang, Conghui Pang, Kehua Wang, Ruihan Wang, Danyang Guo, Zixi Wen

“…A total of 452 fresh embryo transplantation cycles and 1726 Frozen embryo transplantation cycles were carried out.ResultThere were significant differences among the four groups in terms of total Gn dosage, E2, P and LH on trigger day, number of retrieved oocytes, number of 2PN embryos, number of blastocysts, Number of ET and fresh ETR.There is a significant correlation between LH on antagonist administration day and Basal LH Level,LH on trigger day,number of oocytes retrieved,number of 2PN embryos,number of blastocysts, number of ET.Using Fresh ETR,Fresh CPR,OHSS and Cumulative CPR as the criterion respectively, the optimal cut-off value for evaluating LH on antagonist administration day was 4.18IU/L,3.99IU/L,4.63IU/L,4.66IU/L.ConclusionThere was a significant positive correlation between LH on the antagonist administration day and number of oocytes retrieved,number of 2PN embryos,number of blastocysts.LH on the antagonist administration day could predict Fresh CPR,OHSS and Cumulative CPR to some extent.…”
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