The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivoproduced bovine embryos by M. Lopatarova, S. Cech, L. Holy, R. Dolezel

“…Pregnancy rates after transfer of morulae, early blastocysts and expanded blastocysts in Group V compared to Group C (54.5%, 12/22 vs. 56.0%, 14/25; 53.3%, 16/30 vs. 58.1%, 18/31 and 57.7%, 15/26 vs. 48.3%, 14/29) were not different (P > 0.05). …”
Get full text

Leukemia inhibitory factor enhanced the developmental and implantation compatibility of mouse embryos in co-culture with human endometrial epithelial cells by Ali Hosseini, Bahar Movaghar, Showra Amani Abkenari, Hassan Nazari, Mehrdad Bakhtiyari

“…The expression levels of Oct4 and Cdx2 in blastocysts cultured in the presence of LIF were higher than those in blastocysts cultured without LIF. …”
Get full text

Sex determination in bisected bovine embryos and conception rate after the transfer of female demi-embryos by M. Lopatarova, S. Cech, P. Krontorad, L. Holy, J. Hlavicova, R. Dolezel

“…High-quality morulae (n = 100) and early blastocysts (n = 123) obtained from superovulated donors were microsurgically bisected and blastomeres biopsied from one half of bisected embryos were analysed by PCR using specific primers for the Y-chromosome determinant. …”
Get full text

PGT-M, a Useful Tool to Manage the Lynch Syndrome Transmission by Ilaria Listorti, Roberta Manzo, Cristiana Arrivi, Cecilia Mencacci, Anil Biricik, Ermanno Greco, Pierfrancesco Greco

“…In a pre-implantation genetic testing for aneuploidy (PGT-A) + PGT-M cycle, eight blastocysts were formed. Six out of eight blastocysts were affected by the same mother syndrome. …”
Get full text

Successful Live Twin Birth through IVF/ICSI from a Couple with an Infertile Father with Pericentric Inversion of Chromosome 9 (p12q13): A Case with a High Aneuploidy Rate by Ning-Shiuan Ting, Ying-Hsi Chen, Shih-Fen Chen, Pao-Chu Chen

“…PGT-A facilitates the selection of qualified blastocysts for the optimization of live-birth outcomes.…”
Get full text

Comparison of the Morphology and Developmental Potential of Oocytes Obtained from Prepubertal and Adult Domestic and Wild Cats by Joanna Kochan, Agnieszka Nowak, Wiesława Młodawska, Sylwia Prochowska, Agnieszka Partyka, Józef Skotnicki, Wojciech Niżański

“…Moreover, we observed differences in the quantity (28 vs. 39%; <i>p</i> < 0.05) and quality of blastocysts and even greater problems with hatching blastocysts from prepubertal kittens (8 vs. 19%; <i>p</i> < 0.001). …”
Get full text

Randomised double-blind controlled trial of non-invasive preimplantation genetic testing for aneuploidy in in vitro fertilisation: a protocol paper by William Shu Biu Yeung, Ernest H Y Ng, Hiu Yee Heidi Cheng, Judy F C Chow, Kevin K W Lam, Shui Fan Lai

“…In the control group, blastocysts with the best quality morphology will be replaced first. …”
Get full text

Evaluation of Dysfunctional HDL by Myeloperoxidase/Paraoxonase Ratio in Unexplained Infertility Patients Undergoing IVF/ICSI by Kadriye Erdoğan, Nazli Tunca Sanlier, Emine Utlu Özen, Süleyman Erol, Inci Kahyaoğlu, Salim Neselioglu, Özcan Erel, Serra Akar, Yaprak Engin Üstün

“…Number of Grade 1 embryos and the quality of blastocysts were lower in UEI than in the control group (<i>p</i> = 0.024, <i>p</i> = 0.020, respectively), whereas serum MPO/PON ratio was higher in UEI (<i>p</i> = 0.042). …”
Get full text

Derivation of Mouse Parthenogenetic Advanced Stem Cells by Mengyi Wei, Jindun Zhang, Jia Liu, Chaoyue Zhao, Shuo Cao, Xiaojie Yan, Baojiang Wu, Siqin Bao

“…In this study, we established a parthenogenetic epiblast-like stem cell line through culturing parthenogenetic diploid blastocysts in a chemically defined medium containing activin A and bFGF named paAFSCs. …”
Get full text

Usefulness of random-start progestin-primed ovarian stimulation for fertility preservation by Haipeng Huang, Yukiko Itaya, Kouki Samejima, Shunichiro Ichinose, Tatsuya Narita, Shigetaka Matsunaga, Masahiro Saitoh, Yasushi Takai

“…The secondary outcome was the number of vitrified blastocysts per cycle for embryo freezing cases. Results No significant differences were noted in the number of days of stimulation, total dose of gonadotropin preparation, and the number of mature oocytes and vitrified blastocysts. …”
Get full text

Evaluation of Protective Effects of Crocin Onembyo Developing Process in in Vitro Fertilization (IVF)in Cyclophosphamide Treated Mice by F Khan Mohammadi, R Shahrooz, A Ahmadi, M Razi

“…Results: In sham control group a significantly reduction in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and increase the number of arrested developing embryos observed in comparison to control group, and administration of crocin caused significantly increase in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and significantly reduction of arrested developing embryos in comparison to sham control group (P<0.05). …”
Get full text

LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele by Sadie L. Hennig, Bret R. McNabb, Josephine F. Trott, Alison L. Van Eenennaam, James D. Murray

“…Thirty-nine presumptive edited 7-day blastocysts were transferred to 13 synchronized recipient cows resulting in ten pregnancies, five with embryos heterozygous for the dominant PC POLLED allele at the POLLED locus, and five with the recessive pp genotype. …”
Get full text

ICSI in non-male factor infertility patients does not alter metabolomic signature in sibling embryos as evidenced by sensitivity enhanced nuclear magnetic resonance (NMR) spectrosc... by Ameya Jijo, Aswathi Cheredath, Shubhashree Uppangala, Vani Lakshmi R, David Joseph, Huidrom Yaiphaba Meitei, Gitanjali Asampille, Pratap Kumar, Nagana Gowda G A, Guruprasad Kalthur, Borut Kovacic, Satish Kumar Adiga

“…Hence, this study was undertaken to explore if there are any metabolomic differences between IVF and ICSI derived sibling day-5 blastocysts from non-male factor infertility patients. …”
Get full text

Treatment with the proteasome inhibitor MG132 during the end of oocyte maturation improves oocyte competence for development after fertilization in cattle. by Jinyoung You, Eunsong Lee, Luciano Bonilla, Jasmine Francis, Jin Koh, Jeremy Block, Sixue Chen, Peter J Hansen

“…Treatment with MG132 early in maturation prevented progression to meiosis II and reduced fertilization rate and the proportion of oocytes and cleaved embryos that became blastocysts. Conversely, treatment with MG132 late in maturation improved the percentage of oocytes and cleaved embryos that became blastocysts without affecting nuclear maturation or fertilization rate. …”
Get full text

Protease Activity in Different Blastocystis Subtypes in Isfahan by Farzin Khosravi Danesh, Somayeh Mousavi-Mobarakeh, Hossain Yousofi-Darani, Zahra Ghayour Najafabadi

“…The samples were examined microscopically and the blastocysts positive samples were cultured in medium. …”
Get full text

An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo. by Beddington, R, Robertson, E

“…Embryonic stem cells (ES) cells were injected into host blastocysts either in groups of 10-15 cells or as single cells in order to test their developmental potential in the developing embryo. …”

DNA Damage Induction Alters the Expression of Ubiquitin and SUMO Regulators in Preimplantation Stage Pig Embryos by Zigomar da Silva, Werner Giehl Glanzner, Luke Currin, Mariana Priotto de Macedo, Karina Gutierrez, Vanessa Guay, Paulo Bayard Dias Gonçalves, Vilceu Bordignon

“…The relative mRNA abundance of most UBs, DUBs, SUMOs, and deSUMOs was higher in oocytes and early-stage embryos than in blastocysts. Transcript levels for UBs (<i>RNF20</i>, <i>RNF40</i>, <i>RNF114</i>, <i>RNF169</i>, <i>CUL5</i>, <i>DCAF2</i>, <i>DECAF13</i>, and <i>DDB1</i>), DUBs (<i>USP16</i>), and SUMOs (<i>CBX4</i>, <i>UBA2</i> and <i>UBC9</i>), were upregulated in early-stage embryos (D2 and/or D4) compared to oocytes and blastocysts. …”
Get full text

Morphology and molecular phylogeny of trypanorhynchid metacestodes infecting commercial fish of the Mediterranean Sea by K. Morsy, S.B. Dajem, A. Alghamdi, A. El-kott, E. Ibrahim, K. Attia, A. Al-Doaiss, H. El-Mekkawy, N. Sheraba, A. Baiomy, M. Fahmy, F. Shalaby

“…Thirty fish were infected; the infection was recorded as blastocysts embedded in fish flesh. Three different parasitic species were recovered and classified morphologically as Gymnorhynchus isuri, Pseudotobothrium dipsacum and Heteronybelinia estigmena. …”
Get full text

Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does It Matter? by Pin-Yao Lin, Chun-I Lee, En-Hui Cheng, Chun-Chia Huang, Tsung-Hsien Lee, Hui-Hsin Shih, Yi-Ping Pai, Yi-Chun Chen, Maw-Sheng Lee

“…To further confirm the concordance of chromosome constitution between TE and inner cell mass (ICM), 41 remaining embryos designated as mosaic blastocysts donated for research were also analyzed. …”
Get full text

Meiotic arrest with roscovitine and sexual maturity improve competence of mouse oocytes by regulating expression of competence-related genes by Hao CHENG, Xue SUN, Fei CHEN, Liu-Zhu PAN, Guo-Liang WANG, Hong-Jie YUAN, Zhong-Le CHANG, Jing-He TAN

“…Without MAM, while 40% of the 2-cell embryos derived from prepubertal SN oocytes developed into 4-cell embryos, none of the 2-cell embryos derived from prepubertal NSN oocytes did, and while 42% of the 4-cell embryos derived from adult SN oocytes developed into blastocysts, only 1% of the 4-cell embryos derived from prepubertal SN oocytes developed into blastocysts. …”
Get full text