Rescue in vitro maturation may increase the pregnancy outcomes among women undergoing intracytoplasmic sperm injection by Dan-Yu Qin, Hua-Hua Jiang, Qing-Yun Yao, Wen Yao, Xiao-Qiong Yuan, Yi Wang, Tao-Ran Deng, Yao-Yao Du, Xin-Ling Ren, Na Guo, Yu-Feng Li

“…The intermediate reproductive outcomes and pregnancy outcomes were assessed, including the number of normally fertilized embryos, number of cleaved embryos, number of good-quality embryos, number of day-3 available embryos, number of embryos cultured past day-3, number of blastocysts, number of available blastocysts, biochemical pregnancy, miscarriage, clinical pregnancy and live birth. …”
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Intracytoplasmic sperm injection with assisted oocyte activation resulting in successful pregnancies and live birth in couples with globozoospermia: A report of two cases by Puneet Kaur Kochhar, Pranay Ghosh

“…Four oocytes were fertilized and two blastocysts were transferred resulting in a clinical pregnancy. …”
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The Effect of In Vitro Maturation (IVM) Protocol Changes on Measures of Oocyte/Embryo Competence by Bruce I. Rose, Kevin Nguyen

“…Methods: A cohort of 45 women underwent an IVM cycle in which letrozole was used with the theoretical objective of improving the competence of small antral follicles, the oocyte aspiration technique was modified to minimize the time between oocyte aspiration and oocyte identification, and blastocysts were transferred during a subsequent cycle with controlled endometrial development. …”
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The disruptive effects of mastitis on reproduction and fertility in dairy cows by David Wolfenson, Gabriel Leitner, Yaniv Lavon

“…Mastitis, clinical and subclinical, also impairs oocyte competence, reflected in low production of blastocysts. The <em>corpus luteum</em> seems to be insensitive to mastitis, possible due to the use of non-steroidal anti-inflammatory drugs when mastitis is first diagnosed.…”
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A genome-wide RNAi screen identifies determinants of human embryonic stem cells by Chia, Na Yu

“…The derivation of human embryonic cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. …”
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Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse. by Heytens, E, Schmitt-John, T, Moser, J, Jensen, N, Soleimani, R, Young, C, Coward, K, Parrington, J, De Sutter, P

“…The quality of the obtained blastocysts was assessed and embryo transfer was performed to evaluate post-implantation development. …”

In vivo gene transfer into testis and sperm: developments and future application. by Coward, K, Kubota, H, Parrington, J

“…Established methods of creating transgenic animals rely heavily upon injection of DNA into the pronucleus or the injection of transfected embryonic stem cells into blastocysts to form chimeras. Despite the success of these two approaches for making transgenic and knockout animals, concerns remain over costs and the efficiency of transgene integration. …”

Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer by Zhaodi Liao, Jixiang Zhang, Shiyu Sun, Yuzhuo Li, Yuting Xu, Chunyang Li, Jing Cao, Yanhong Nie, Zhuoyue Niu, Jingwen Liu, Falong Lu, Zhen Liu, Qiang Sun

“…Our findings reveal a widespread decrease in DNA methylation and the loss of imprinting in maternally imprinted genes within SCNT monkey blastocysts. This loss of imprinting persists in SCNT embryos cultured in-vitro until E17 and in full-term SCNT placentas. …”
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Limitation of adipose tissue by the number of embryonic progenitor cells by Kristina Hedbacker, Yi-Hsueh Lu, Olof Dallner, Zhiying Li, Gulya Fayzikhodjaeva, Kıvanç Birsoy, Chiayun Han, Chingwen Yang, Jeffrey M Friedman

“…We addressed this by injecting labeled wild-type embryonic stem cells into blastocysts derived from lipodystrophic A-ZIP transgenic mice, which have a genetic block in adipogenesis. …”
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Lessons Learned from Somatic Cell Nuclear Transfer by Chantel Gouveia, Carin Huyser, Dieter Egli, Michael S. Pepper

“…The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic cloning, or for the purpose of reproductive cloning. …”
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Successful production of offspring derived from mouse zygotes vitrified with carboxylated ε-poly-L-lysine and polyvinyl alcohol without serum by Midori ISHII, Maki KAMOSHITA, Yoshihiro KURIHARA, Kazuaki MATSUMURA, Suong-Hyu HYON, Junya ITO, Naomi KASHIWAZAKI

“…When COOH-PLL was added to the vitrification solutions, zygotes vitrified with solutions containing 0.01% PVA (PV0.01) and those vitrified in a control solution containing FCS (75.6%) developed into blastocysts (78.4%). In addition, there were no significant differences in the ability to develop to term between the control solution (46.6%) and PV0.01 (44.1%) groups. …”
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MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway by Karina Cañón-Beltrán, Yulia N Cajas, Vasileios Almpanis, Sandra Guisado Egido, Alfonso Gutierrez-Adan, Encina M González, Dimitrios Rizos

“…Embryos at ≥ 16-cells and D7 blastocysts (BD7) were collected to examine the mRNA abundance of transcripts linked to the TGF-β pathway (TGFBR2, SMAD1, SMAD2, SMAD3, SMAD5, BMPR2, RPS6KB1, POU5F1, NANOG), total cell number (TC), trophectoderm (TE), and inner cell mass (ICM) were also evaluated. …”
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Single-Step Genome Editing of Small Ruminant Embryos by Electroporation by Ahmed K. Mahdi, Juan F. Medrano, Pablo J. Ross

“…Electroporation of sheep zygotes 6 h after fertilization with settings that included short high-voltage (poring) and long low-voltage (transfer) pulses was efficient at producing SOCS2 knock-out blastocysts. The mutation rate after CRISPR/Cas9 electroporation was 95.6% ± 8%, including 95.4% ± 9% biallelic mutations; which compared favorably to 82.3% ± 8% and 25% ± 10%, respectively, when using microinjection. …”
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DNA polymerase delta is required for early mammalian embryogenesis. by Arikuni Uchimura, Yuko Hidaka, Takahiro Hirabayashi, Masumi Hirabayashi, Takeshi Yagi

“…Although Pold1(-/-) blastocysts appeared normal, their in vitro culture showed defects in outgrowth proliferation and DNA synthesis and frequent spontaneous apoptosis, indicating Poldelta participates in DNA replication during mouse embryogenesis. …”
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Developmental Acquisition of <i>p53</i> Functions by Sushil K. Jaiswal, Sonam Raj, Melvin L. DePamphilis

“…Here we attempt to reconcile disparate data into justifiable conclusions predicated on reports that <i>p53</i>-dependent transcription is first detected in late mouse blastocysts, that <i>p53</i> activity first becomes potentially lethal during gastrulation, and that apoptosis does not depend on <i>p53</i>. …”
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First sex modification case in equine cloning by Mariana Suvá, Victoria Helga Arnold, Elisabet Astrid Wiedenmann, Roberto Jordan, Emanuel Galvagno, Marcela Martínez, Gabriel Damián Vichera

“…After a single SCNT procedure, 16 blastocysts were obtained and transferred to eight recipient mares, resulting in the birth of two clones: one male and one female. …”
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Legislation governing pluripotent stem cells in South Africa by Michael Pepper, C Gouveia, M Slabbert

“…Pluripotent cells derived from the inner cell mass of blastocysts (either from in vitro fertilisation or following somatic cell nuclear transfer) are called embryonic stem (ES) cells, while those derived by reprogramming adult cells are called induced pluripotent stem (iPS) cells. …”
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First sex modification case in equine cloning. by Mariana Suvá, Victoria Helga Arnold, Elisabet Astrid Wiedenmann, Roberto Jordan, Emanuel Galvagno, Marcela Martínez, Gabriel Damián Vichera

“…After a single SCNT procedure, 16 blastocysts were obtained and transferred to eight recipient mares, resulting in the birth of two clones: one male and one female. …”
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In vivo gene transfer into testis and sperm: Developments and future application by Coward, K, Kubota, H, Parrington, J

“…Established methods of creating transgenic animals rely heavily upon injection of DNA into the pronucleus or the injection of transfected embryonic stem cells into blastocysts to form chimeras. Despite the success of these two approaches for making transgenic and knockout animals, concerns remain over costs and the efficiency of transgene integration. …”

Improved prediction of clinical pregnancy using artificial intelligence with enhanced inner cell mass and trophectoderm images by Hyung Min Kim, Taehoon Ko, Hyoeun Kang, Sungwook Choi, Jong Hyuk Park, Mi Kyung Chung, Miran Kim, Na Young Kim, Hye Jun Lee

“…In this retrospective study, we included static images of 2555 day-5-blastocysts from seven in vitro fertilization centers in South Korea. …”
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