Monoallelic maternal expression of <it>STAT5A </it>affects embryonic survival in cattle by Monson Ricky L, Maltecca Christian, Khatib Hasan, Schutzkus Valerie, Rutledge Jack J

“…Furthermore, the occurrence of monoallelic maternal expression of <it>STAT5A </it>was significantly higher in blastocysts, while paternal expression was more frequent in degenerative embryos.…”
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Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse. by Davies, T, Fairchild, P

“…These ESCs displayed a normal karyotype that was robustly stable during long-term culture, were capable of forming teratomas in vivo and germline competent chimeras after injection into recipient blastocysts. Further, these novel ESC lines efficiently formed embryoid bodies in vitro and could be directed in their differentiation along the dendritic cell lineage, thus illustrating their potential application to the generation of cell types of relevance to the pathogenesis of type I diabetes.…”

Translational inhibition of cyclin B1 and appearance of cyclin D1 very early in the differentiation of mouse trophoblast giant cells. by Palazón, L, Davies, T, Gardner, R

“…The latter was ruled out by examining blastocysts which had been cultured in the presence of the proteasome inhibitor N-acetyl-leu-leu-norleucinal followed by immunostaining for cyclin B1. …”

Hysteroscopic Endometrial Fundal Incision in Oocyte Recipients before Embryo Transfer May Improve Reproductive Outcomes: A Prospective Study by Robert Najdecki, Nikolaos Peitsidis, Ioannis Tsakiridis, Georgios Michos, Evi Timotheou, Tatiana Chartomatsidou, Apostolos Athanasiadis, Evangelos Papanikolaou

“…Bothgroups were similar in terms of age, years of infertility, duration of hormone replacement treatment (HRT) and numberof blastocysts transferred. In the EFI group, minor anomalies were detected and treated in 6.1% (n=7) of the participants.Moreover, pregnancy test was positive in 73.7% of the women in the hysteroscopy group compared to 57.8% in the nonhysteroscopygroup (P=0.004). …”
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Identification of the Lineage Markers and Inhibition of <i>DAB2</i> in In Vitro Fertilized Porcine Embryos by Jong-Nam Oh, Mingyun Lee, Gyung Cheol Choe, Dong-Kyung Lee, Kwang-Hwan Choi, Seung-Hun Kim, Jinsol Jeong, Chang-Kyu Lee

“…We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. …”
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ZFP57 regulates DNA methylation of imprinted genes to facilitate embryonic development of somatic cell nuclear transfer embryos in Holstein cows by Tong Yu, Ru Meng, Weijia Song, Hongzheng Sun, Quanli An, Chengtu Zhang, Yong Zhang, Jianmin Su

“…Our results demonstrated that overexpression of ZFP57 increased total and trophectoderm cell numbers and the ratio of inner cell mass to total cells, reduced the apoptosis rate and significantly enhanced the development of SCNT blastocysts in vitro, ultimately achieving a degree of methylation similar to that in in vitro fertilization embryos. …”
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Tetrabromobisphenol Exposure Impairs Bovine Oocyte Maturation by Inducing Mitochondrial Dysfunction by Jing Guo, Chang-Guo Min, Kai-Yan Zhang, Cheng-Lin Zhan, Yu-Chan Wang, Sheng-Kui Hou, Xin Ma, Wen-Fa Lu

“…In addition, the developmental competence of oocytes and the quality of blastocysts were also reduced after TBBPA treatment. …”
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#91 : Development of a Combined Artificial Intelligence Score for Evaluating Both Embryo Ploidy and Viability to Aid in Embryo Selection During IVF by Sonya Diakiw, Matthew VerMilyea, Michelle Perugini, Tuc Nguyen, Don Perugini, Jonathan Hall

“…Previous studies described development of AI for selecting embryos likely to be euploid (genetics AI), or likely to lead to clinical pregnancy (viability AI), based on analysis of images of blastocysts on day 5 of development. The aim of this study was to determine if a combination of these AI scores could be used to effectively evaluate both outcomes. …”
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Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development by Kihae Ra, Hyun Ju Oh, Eun Young Kim, Sung Keun Kang, Jeong Chan Ra, Eui Hyun Kim, Se Chang Park, Byeong Chun Lee

“…AMSC-CM (10%) was optimal for overall preimplantation embryo developmental processes and upregulated the expression of FoxOs and their downstream antioxidants in blastocysts (BL). Subsequently, compared to adipose-derived mesenchymal stem cell (ASC)-CM, AMSC-CM enhanced antioxidant gene expression and intracellular GSH levels in the BL. …”
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Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos by Wen-Jie Jiang, Song-Hee Lee, Geun Heo, Hak Jae Chung, Eun Seok Cho, Soo Jin Sa, Shinichi Hochi, Xiang-Shun Cui

“…The mRNA level of YBX1 was found to be highly expressed at the four-cell (4C) stage in porcine embryos compared with one-cell (1C) and two-cell (2C) stages. The number of blastocysts was reduced following YBX1 knockdown. Notably, YBX1 knockdown decreased the phosphatase and tensin homolog-induced kinase 1 (PINK1) and parkin RBR E3 ubiquitin protein ligase (PRKN) mRNA levels. …”
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The Quality and Fertilizing Potential of Red Deer (<i>Cervus elaphus</i> L.) Epididymal Spermatozoa Stored in a Liquid State by Anna Dziekońska, Magdalena Koziorowska-Gilun, Władysław Kordan, Nicoletta M. Neuman, Angelika M. Kotlarczyk, Anna J. Korzekwa

“…The results of IVF and AI demonstrated that the fertilizing potential of spermatozoa differs between days of storage. The percentage of blastocysts was higher when oocytes were fertilized on D1 (17.4 %) compared to D7 (8.5%) and D10 sperm (10.5%). …”
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A New Strategy for Constructing Mouse Models of Complex Diseases: Semi-cloning Technology Based on Sperm-like Haploid Embryonic Stem Cells by LAI Suomei, DING Yifu, LI Jinsong

“…Sperm-like haESCs are novel haESCs derived from mouse parthenogenetic blastocysts. Sperm-like haESCs only contain paternal genetic material, and their sex chromosome is the X chromosome. …”
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Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization by Zhuo Song, Bo Li, Meng-Yuan Li, Jia-Mei Luo, Yu-Qi Hong, Yu-Ying He, Si-Ting Chen, Zhen-Shan Yang, Chen Liang, Zeng-Ming Yang

“…Caveolin-1 overexpression and siRNA suppress and upregulate <i>IGFBP1</i> expression under in vitro decidualization, respectively. Blastocysts-derived tumor necrosis factor α (TNFα) and human Chorionic Gonadotropin (hCG) regulate Caveolin-1 in mouse and human decidual cells, respectively. …”
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Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes by Jae Kyun Park, Ju Hee Lee, Eun A Park, Hyunjung J. Lim, Sang Woo Lyu, Woo Sik Lee, Jayeon Kim, Haengseok Song

“…Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future.…”
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Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes by Udayanga Sanath Kankanam Gamage, Shu Hashimoto, Yuki Miyamoto, Tatsuya Nakano, Masaya Yamanaka, Akiko Koike, Manabu Satoh, Yoshiharu Morimoto

“…We found that, compared to their counterpart, mitochondria supplementation significantly improved development from 2-cell embryos to blastocysts (56.8% vs. 38.2%) and ATP production in 2-cell embryos (905.6 & 561.1 pmol), while reactive oxygen species levels were comparable. …”
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Earlier second polar body transfer and further mitochondrial carryover removal for potential mitochondrial replacement therapy by Wenzhi Li, Xiaoyu Liao, Kaibo Lin, Renfei Cai, Haiyan Guo, Meng Ma, Yao Wang, Yating Xie, Shaozhen Zhang, Zhiguang Yan, Jiqiang Si, Hongyuan Gao, Leiwen Zhao, Li Chen, Weina Yu, Chen Chen, Yun Wang, Yanping Kuang, Qifeng Lyu

“…The results showed that our scheme could produce a nearly normal proportion of normal‐karyotype blastocysts with further reduced mitochondrial carryover, both in mice and humans. …”
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Selection of Boar Sperm by Reproductive Biofluids as Chemoattractants by Luis Alberto Vieira, Alessia Diana, Cristina Soriano-Úbeda, Carmen Matás

“…Regarding the in vitro fertility, the spermatozoa attracted by FF produced higher rates of penetration of oocytes and development of expanded blastocysts. In conclusion, porcine reproductive biofluids show an in vitro chemotactic effect on spermatozoa and modulate their fertilizing potential.…”
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Jarid2 Coordinates Nanog Expression and PCP/Wnt Signaling Required for Efficient ESC Differentiation and Early Embryo Development by David Landeira, Hakan Bagci, Andrzej R. Malinowski, Karen E. Brown, Jorge Soza-Ried, Amelie Feytout, Zoe Webster, Elodie Ndjetehe, Irene Cantone, Helena G. Asenjo, Neil Brockdorff, Thomas Carroll, Matthias Merkenschlager, Amanda G. Fisher

“…In addition, we show that ESCs lacking Jarid2 or Wnt9a/Prickle1/Fzd2 or overexpressing Nanog induce multiple ICM formation when injected into normal E3.5 blastocysts. These data describe a previously unrecognized role for Jarid2 in regulating a core pluripotency and Wnt/PCP signaling circuit that is important for ESC differentiation and for pre-implantation development.…”
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Exploration of the interchromosomal effects in preimplantation genetic testing for structural rearrangements based on next‐generation sequencing by Junmei Fan, Xueluo Zhang, Yanhua Chen, Junkun Zhang, Lei Zhang, Xingyu Bi, Jinbao Wang, Xiang Huang, Meiqin Yan, Xueqing Wu

“…Results Among the 283 couples who participated in this study, 1076 blastocysts from 352 cycles were collected, and 246 de novo abnormal embryos were included. …”
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An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells by Jianying Guo, Dacheng Ma, Rujin Huang, Jia Ming, Min Ye, Kehkooi Kee, Zhen Xie, Jie Na

“…Interestingly, an elevated NANOG level promoted naïve pluripotent gene expression, enhanced cell survival and clonogenicity, and enabled hESCs to integrate with the inner cell mass (ICM) of mouse blastocysts in vitro. Thus, iVPR cells provide a convenient platform for gene function studies as well as high-throughput screens in hPSCs.…”
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