Efficient Reprogramming of Mouse Embryonic Stem Cells into Trophoblast Stem-like Cells via Lats Kinase Inhibition by Yake Gao, Wenrui Han, Rui Dong, Shu Wei, Lu Chen, Zhaolei Gu, Yiming Liu, Wei Guo, Fang Yan

“…Mouse zygotes undergo multiple rounds of cell division, resulting in the formation of preimplantation blastocysts comprising three lineages: trophectoderm (TE), epiblast (EPI), and primitive endoderm (PrE). …”
Get full text

Feasibility of combining short tandem repeats (STRs) haplotyping with preimplantation genetic diagnosis (PGD) in screening for beta thalassemia by Vu Viet Ha Vuong, Thinh Huy Tran, Phuoc-Dung Nguyen, Nha Nguyen Thi, Phuong Le Thi, Dang Thi Minh Nguyet, Manh-Ha Nguyen, The-Hung Bui, Thanh Van Ta, Van-Khanh Tran

“…With 231 embryos, 168 blastocysts were formed (formation rate of 72.73%), and 88 were biopsied and examined with STRs haplotyping and pedigree analysis. …”
Get full text

Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and in vitro embryo production in Japanese Black cows by Kensuke TOMITA, Toshimichi ISHII, Natsumi ENDO, Tomomi TANAKA

“…The proportion of cleaved oocytes was lower in the HD group than in the MD group (P < 0.05); consequently, there was no significant difference in the number of blastocysts obtained between the HD and MD groups. The present findings suggest that high-energy diets can promote follicular growth in parallel with an increase in plasma concentrations of insulin, but have a detrimental effect on the quality of oocytes with the OPU-IVF procedure in Japanese Black cows.…”
Get full text

Protective Effects of Hydroalcoholic Extract of Achillea Millefolium on In-vitro Fertilization in Streptozotocin-Induced Diabetic Male Mice by Mohammadreza Hosseinchi, Gholamreza Najafi

“…Results: The sperm count, percentage of live sperms, IVF rate, percentage of two-cell embryos, and percentage of blastocysts decreased significantly in the diabetic group compared to the control group. …”
Get full text

The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period by Hitomi Matsuzaki, Shokichi Sugihara, Keiji Tanimoto

“…In contrast, the mouse IG-DMR, the methylation of which is acquired during spermatogenesis at the endogenous locus, did not acquire methylation in the sperm of TgM, yet became highly methylated in blastocysts after fertilization, but only when the transgene was paternally inherited. …”
Get full text

Seasonal Effect on Developmental Competence, Oxidative Status and Tubulin Assessment of Prepubertal Ovine Oocyte by Elisa Serra, Sergio Domenico Gadau, Giovanni Giuseppe Leoni, Salvatore Naitana, Sara Succu

“…The results on embryo production, as a percentage of first divisions and number of blastocysts obtained, were significantly higher in oocytes collected in the spring. …”
Get full text

Clinical outcome of intrauterine infusion of platelet‐rich plasma in patients with recurrent implantation failure by Yihsien Enatsu, Noritoshi Enatsu, Kanako Kishi, Junko Otsuki, Toshiroh Iwasaki, Eri Okamoto, Shoji Kokeguchi, Masahide Shiotani

“…Methods The authors retrospectively analyzed 54 ET cycles involving frozen and thawed high‐quality blastocysts after intrauterine PRP infusion between September 2019 and November 2020. …”
Get full text

#123 : Caveolin-1 Function and Regulation in Mouse Uterus During Early Pregnancy and Under Human In Vitro Decidualization by Song Zhuo

“…In mESCs, Caveolin-1 seems to play a different role in decidualization, over-expression of Caveolin-1 could promote decidualization and senescence in mESCs, but siRNA depressed it. Blastocysts-derived TNF and hCG stimulated Caveolin-1 in mice and depressed it in human decidual cells, respectively. …”
Get full text

<it>In silico </it>identification of a core regulatory network of OCT4 in human embryonic stem cells using an integrated approach by Adjaye James, Lehrach Hans, Vingron Martin, Bais Abha S, Chavez Lukas, Herwig Ralf

“…<p>Abstract</p> <p>Background</p> <p>The transcription factor OCT4 is highly expressed in pluripotent embryonic stem cells which are derived from the inner cell mass of mammalian blastocysts. Pluripotency and self renewal are controlled by a transcription regulatory network governed by the transcription factors OCT4, SOX2 and NANOG. …”
Get full text

A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and protein... by Chou Chi-Hsien, Yu Sung-Liang, Singh Sher, Tsai Zong-Yun, Li Steven

“…<p>Abstract</p> <p>Background</p> <p>Human embryonic stem (hES) cell lines were derived from the inner cell mass of human blastocysts, and were cultured on mouse embryonic fibroblast (MEF) feeder to maintain undifferentiated growth, extensive renewal capacity, and pluripotency. …”
Get full text

Assessment of Fertility in Female Offspring of Stressed Male Rats Treated with Sulpiride by Shahla Amiri, Farrin Babaei-Balderlou, Gholamreza Najafi

“…Results: The weight of the body and ovaries, percentage of egg cells, bicellular embryos, blastocysts, and hatched embryos after IVF, significantly reduced in offspring of stressed male rats compared with control group (P<0.05). …”
Get full text

Review: Progress in producing chimeric ungulate livestock for agricultural applications by Alba V. Ledesma, Maci L. Mueller, Alison L. Van Eenennaam

“…Despite the successful production of chimeras with germ cells generated from pluripotent donor stem cells injected into preimplantation-stage blastocysts in model species, there are no documented cases of this occurring in livestock. …”
Get full text

Embryo Cryopreservation in a Patient with Sickle Cell Disease Utilizing Letrozole and Enoxaparin: A Case Report by Stephanie J. Gunderson, Nina Snowden, Joshua J. Field

“…Ten mature oocytes were retrieved and a total of 10 blastocysts were cryopreserved. The patient required pain medication and intravenous fluids after oocyte retrieval due to pain but had significant improvement at the scheduled post-operative day one follow-up. …”
Get full text

Effects of bovine spermatozoa preparation on embryonic development in vitro by Cergolj Marijan, Makek Zdenko, Getz Iva, Karadjole Martina, Samardzija Marko, Dobranic Tomislav

“…Also, total cell number and embryo differential staining (inner cell mass and trophectoderm cells) of Day 7 morulas and blastocysts showed that BoviPure^®treated sperm displayed higher quality embryos compared to swim up method (P < 0.05). …”
Get full text

Feasibility of combining short tandem repeats (STRs) haplotyping with preimplantation genetic diagnosis (PGD) in screening for beta thalassemia. by Vu Viet Ha Vuong, Thinh Huy Tran, Phuoc-Dung Nguyen, Nha Nguyen Thi, Phuong Le Thi, Dang Thi Minh Nguyet, Manh-Ha Nguyen, The-Hung Bui, Thanh Van Ta, Van-Khanh Tran

“…With 231 embryos, 168 blastocysts were formed (formation rate of 72.73%), and 88 were biopsied and examined with STRs haplotyping and pedigree analysis. …”
Get full text

#292 : Combination of Hormone Replacement Therapy and Ovarian Stimulation as Endometrial Preparation Strategy for Refractory Thin Endometrium by Uma Mariappen

“…She then underwent an IVF cycle and obtained five blastocysts. We struggled with thin ET with one failed frozen embryo transfer (FET) and two abandoned cycles. …”
Get full text

Self-Organization of Stem Cell Colonies and of Early Mammalian Embryos: Recent Experiments Shed New Light on the Role of Autonomy vs. External Instructions in Basic Body Plan Devel... by Hans-Werner Denker

“…The present article focuses on recent reports using the term self-organization in the context of studies on embryogenesis, specifically addressing pattern formation processes in human blastocysts attaching in vitro, or in colonies of pluripotent stem cells (“gastruloids”). …”
Get full text

Analysis of histone variant H3.3 function in embryonic development and stem cell differentiation by Tai, Joyce An Yi

“…Preliminary results showed that mutation of H3.3A K27 may delay embryonic development by approximately 1 cleavage-stage while mutation of H3.3A K4 may lead to improper formation of ICM in blastocysts. However, due to the insufficient statistical evidence to support the abnormal ICM formation induced in embryos expressing H3.3A K4A mutants, more experimental repeats have to be performed. …”
Get full text

Increasing knock-in efficiency in mouse zygotes by transient hypothermia by Bouchareb, A, Biggs, D, Alghadban, S, Preece, C, Davies, B

“…The efficiency of gene editing with and without the cold shock was first assessed by genotyping blastocysts. As a proof of concept, we then confirmed that the modified embryo culture conditions were compatible with live births by targeting the coat colour gene Tyrosinase and observing the repair of the albino mutation. …”

Generation and characterization of transgenic mice with the full-length human DMD gene. by 't Hoen, P, de Meijer, E, Boer, J, Vossen, R, Turk, R, Maatman, R, Davies, K, van Ommen, G, van Deutekom, J, den Dunnen, J

“…Yeast spheroplasts containing an artificial chromosome with the full-length hDMD gene were fused with mouse embryonic stem cells and were subsequently injected into mouse blastocysts to produce transgenic hDMD mice. Human-specific PCR, Southern blotting, and fluorescent in situ hybridization techniques demonstrated the intactness and stable chromosomal integration of the hDMD gene on mouse chromosome 5. …”