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1721
Prevalence of Cryptosporidium spp. and Blastocystis hominis in faecal samples among diarrheic HIV patients in Medan, Indonesia
Published 2021-02-01“…CD4 count showed a significant relationship with Cryptosporidium spp. infection, but not with Blastocystic hominis. Microscopic examination is still the most reliable technique to diagnose the parasites in faecal samples.…”
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1722
Effects of growth factors during in vitro culture of mouse and rat embryos
Published 2015-12-01“…Supplementation of the culture medium with EGF increased the percentage of developing blastocysts in OXYS/Icgn rat strain, while GM-CSF had no effect. …”
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1723
#322 : A Rare Case Report of Successfull Live Twin Birth in a Couple Who are Carriers of Mucopolysaccharidoses VII-Sly Syndrome
Published 2023-12-01“…The resultant 2 carrier blastocysts were transferred. Post transfer Beta Hcg was positive. …”
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1724
Modulation of Apoptosis and Autophagy by Melatonin in Juglone-Exposed Bovine Oocytes
Published 2023-04-01“…We found that administration of melatonin during the maturation of oocytes under juglone stress significantly improved the cleavage rate, 8-16 cell-stage embryos and day-8 blastocysts when compared to the sole juglone treatment. …”
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1725
Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism
Published 2022-07-01“…Organotypic culture of human fetal testis has achieved fertilization-competent spermatids followed by blastocysts development. This study focuses on whether the organotypic culture of testicular tissue from infant boys with cryptorchidism could support the development of spermatogonia and somatic cells. …”
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1726
Hormonal parameters and embryological outcomes of in vitro fertilisation/intra cytoplasmic sperm injection cycles in women using dydrogesterone for the prevention of premature lute...
Published 2022-11-01“…We evaluated the starting and total dose of gonadotropins, duration of stimulation, serum levels of LH, oestradiol and progesterone on Day 1 and 6 of stimulation and the day of trigger administration, number of retrieved and mature oocytes, fertilization rate, and the number of blastocysts and cryopreserved embryos. Results. There were no significant differences in most hormonal parameters, characteristics of the ovarian stimulation and embryological outcomes between the two groups. …”
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1727
The Effect of COVID-19 Severity, Associated Serum Autoantibodies and Time Interval after the Disease on the Outcomes of Fresh Oocyte ART Cycles in Non-Vaccinated Patients
Published 2023-06-01“…However, when oocyte retrieval was performed less than 180 days after COVID-19, a higher proportion of poor-quality blastocysts was obtained (<i>p</i> = 0.006). A high risk of early miscarriage was found in the patients with moderate COVID-19. …”
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1728
CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics
Published 2023-04-01“…We verified that genome-editing did not interfere with the Gnao1 mRNA or Gαo protein synthesis and did not alter localization of the protein in the brain structures. The analysis of blastocysts revealed the off-target activity of the CRISPR/Cas9 complexes; however, no modifications of the predicted off-target sites were detected in the founder mouse. …”
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1729
The vertebrate- and testis- specific transmembrane protein C11ORF94 plays a critical role in sperm-oocyte membrane binding
Published 2022-09-01“…In consistence, when the sperm of C11orf94-deficient mice were microinjected into the oocyte cytoplasm, fertilized oocytes were obtained and developed normally to blastocysts. Proteomics analysis revealed that C11orf94 influenced the expression of multiple gene products known to be indispensable for sperm-oocyte binding and fusion, including IZUMO1, EQTN and CRISP1. …”
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1730
Donor template delivery by recombinant adeno-associated virus for the production of knock-in mice
Published 2024-02-01“…By comparing our production data against genetically altered knock-in mice generated via gene targeting in embryonic stem cells and their microinjection into blastocysts, we assess the animal cost of the two methods. …”
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1731
Oxidative damage contributes to bisphenol S-induced development block at 2-cell stage preimplantation embryos in mice through inhibiting of embryonic genome activation
Published 2023-06-01“…The results showed that 10–6 mol/L BPS exposure delayed the blastocysts stage, and exposure to 10–4 mol/L BPS induced 2-cell block in mice preimplantation embryos. …”
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1732
SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo
Published 2021-12-01“…The impact of COS protocols on endometrial gene expression profile of SARS-CoV-2-associated receptors and proteases is minimal, suggesting no additional potential risks during stimulated ART procedure. In blastocysts, <i>ACE2</i>, <i>BSG</i>, <i>CTSL</i>, <i>CTSA</i> and <i>FURIN</i> were detectable in the entire cohort at high expression level. …”
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1733
Developmental competence of interspecies cloned embryos produced using cells from large Japanese field mice (Apodemus speciosus) and oocytes from laboratory mice (Mus musculus dome...
Published 2020-03-01“…Only a small number of iSCNT embryos developed to the 4-cell (0–4%) and blastocysts (0–1%) stages under sequential treatment using trichostatin A (TSA) and vitamin C (VC) supplemented with deionized bovine serum albumin (d-BSA). …”
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1734
Evaluation of Cardiovascular Toxicity of Folic Acid and 6S-5-Methyltetrahydrofolate-Calcium in Early Embryonic Development
Published 2022-12-01“…We then analyzed the differently expressed genes in in vitro fertilized murine blastocysts cultured with FA and MTHF-Ca. By using gene-expression profiling, we identified a novel gene in mice that encodes an essential eukaryotic translation initiation factor (<i>Eif1ad7</i>). …”
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1735
#264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development
Published 2023-12-01“…Results: Using the proposed method, live mouse embryos were monitored at different developmental stages, ranging from 2-cell embryos to expanded blastocysts. The measured 3D RI maps of the developing embryos reveal 3D landscape of subcellular features inside the embryos (Fig. 1). …”
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1736
A cost-effectiveness analysis of the use of highly purified human menopausal gonadotropins in assisted reproductive technology programs in gonadotropinreleasing hormone agonist or...
Published 2022-04-01“…Prescription of highly purified HMG (Menopur Multidose 1200 IU) to stimulate ovarian function in patients of different ages is accompanied by an adequate number of mature oocytes, good quality blastocysts, as well as satisfactory indicators of clinical pregnancy. …”
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1737
Facts about Stem Cells and Importance of Them
Published 2014-05-01“…In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. …”
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1738
#248 : Maternal Hypercholesterolemia Would Increase the Incidence of Embryo Aneuploidy in Couples with Recurrent Implantation Failure
Published 2023-12-01“…Compared with the NonH group, SHC group had a significantly increased aneuploid embryo rate [48.3% vs. 36.7%, P=0.006; adjusted OR (95% CI) = 1.52(1.04-2.22)], as well as a reduced number of good-quality blastocysts [3.00±2.29 vs. 3.74±2.77, P=0.033]. The SHC group showed a lower cumulative live-birth rate (47.0% vs. 40.0%), good birth outcome (37.2% vs. 34.5%) and a higher risk of clinical pregnancy loss (11.1% vs. 17.9%) but did not reach statistical significance. …”
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1739
Influence of different environments on oocyte maturation and development of bovine embryos in vitro
Published 2022-09-01“…Thus, at 48 hours of cultivation (the initial stage of embryo development), 64.3 ± 1.0 and 60.3 ± 1.4% of 2-8 cell embryos were obtained, and on Day 8 – 25.3 ± 1.0 and 20.0 ± 0.6% of blastocysts, respectively. The results of a comparison of bovine embryo culture protocols showed that when using both Minitube and Origio media, the percentage of division and the percentage of resulting embryos corresponded to their known values. …”
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1740
Efficient reprogramming of naïve-like induced pluripotent stem cells from porcine adipose-derived stem cells with a feeder-independent and serum-free system.
Published 2014-01-01“…Furthermore, nuclear transfer embryos from naïve-like piPSCs could develop to blastocysts with improved quality. Thus, we provided an efficient protocol for generating naïve-like piPSCs from pADSCs in a feeder-independent and serum-free system with controlled regulation of exogenous genes, which may facilitate optimization of culture media and the production of transgenic pigs.…”
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