Prevalence of Cryptosporidium spp. and Blastocystis hominis in faecal samples among diarrheic HIV patients in Medan, Indonesia by Hemma Yulfi, Muhammad Fakhrur Rozi, Yunilda Andriyani, Dewi Masyithah Darlan

“…CD4 count showed a significant relationship with Cryptosporidium spp. infection, but not with Blastocystic hominis. Microscopic examination is still the most reliable technique to diagnose the parasites in faecal samples.…”
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Effects of growth factors during in vitro culture of mouse and rat embryos by E. Yu. Brusentsev, T. N. Igonina, I. N. Rozhkova, D. S. Ragaeva, S. Ya. Amstislavsky

“…Supplementation of the culture medium with EGF increased the percentage of deve­loping blastocysts in OXYS/Icgn rat strain, while GM-CSF had no effect. …”
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#322 : A Rare Case Report of Successfull Live Twin Birth in a Couple Who are Carriers of Mucopolysaccharidoses VII-Sly Syndrome by Vasundara Jagannathan, Srinidhi Ranganathan

“…The resultant 2 carrier blastocysts were transferred. Post transfer Beta Hcg was positive. …”
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Modulation of Apoptosis and Autophagy by Melatonin in Juglone-Exposed Bovine Oocytes by Marwa El-Sheikh, Ahmed Atef Mesalam, Seon-Min Kang, Myeong-Don Joo, Seham Samir Soliman, Atif Ali Khan Khalil, Mi-Jeong Ahn, Il-Keun Kong

“…We found that administration of melatonin during the maturation of oocytes under juglone stress significantly improved the cleavage rate, 8-16 cell-stage embryos and day-8 blastocysts when compared to the sole juglone treatment. …”
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Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism by Danyang Wang, Simone Hildorf, Elissavet Ntemou, Linn Salto Mamsen, Lihua Dong, Susanne Elisabeth Pors, Jens Fedder, Erik Clasen-Linde, Dina Cortes, Jørgen Thorup, Claus Yding Andersen

“…Organotypic culture of human fetal testis has achieved fertilization-competent spermatids followed by blastocysts development. This study focuses on whether the organotypic culture of testicular tissue from infant boys with cryptorchidism could support the development of spermatogonia and somatic cells. …”
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Hormonal parameters and embryological outcomes of in vitro fertilisation/intra cytoplasmic sperm injection cycles in women using dydrogesterone for the prevention of premature lute... by Irina E. Korneeva, Alla I. Kovalchuk, Tatiana A. Nazarenko, Elena V. Mityurina, Svetlana G. Perminova, Aleksei V. Bachurin, Tatiana Yu. Ivanets

“…We evaluated the starting and total dose of gonadotropins, duration of stimulation, serum levels of LH, oestradiol and progesterone on Day 1 and 6 of stimulation and the day of trigger administration, number of retrieved and mature oocytes, fertilization rate, and the number of blastocysts and cryopreserved embryos. Results. There were no significant differences in most hormonal parameters, characteristics of the ovarian stimulation and embryological outcomes between the two groups. …”
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The Effect of COVID-19 Severity, Associated Serum Autoantibodies and Time Interval after the Disease on the Outcomes of Fresh Oocyte ART Cycles in Non-Vaccinated Patients by Nataliya V. Dolgushina, Irina V. Menzhinskaya, Daria M. Ermakova, Natalia A. Frankevich, Valentina V. Vtorushina, Gennady T. Sukhikh

“…However, when oocyte retrieval was performed less than 180 days after COVID-19, a higher proportion of poor-quality blastocysts was obtained (<i>p</i> = 0.006). A high risk of early miscarriage was found in the patients with moderate COVID-19. …”
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CRISPR/Cas9-generated mouse model with humanizing single-base substitution in the Gnao1 for safety studies of RNA therapeutics by Anna V. Polikarpova, Anna V. Polikarpova, Tatiana V. Egorova, Tatiana V. Egorova, Evgenii A. Lunev, Evgenii A. Lunev, Evgenii A. Lunev, Alexandra A. Tsitrina, Svetlana G. Vassilieva, Svetlana G. Vassilieva, Irina M. Savchenko, Irina M. Savchenko, Yuliya Y. Silaeva, Alexey V. Deykin, Alexey V. Deykin, Alexey V. Deykin, Maryana V. Bardina, Maryana V. Bardina, Maryana V. Bardina

“…We verified that genome-editing did not interfere with the Gnao1 mRNA or Gαo protein synthesis and did not alter localization of the protein in the brain structures. The analysis of blastocysts revealed the off-target activity of the CRISPR/Cas9 complexes; however, no modifications of the predicted off-target sites were detected in the founder mouse. …”
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The vertebrate- and testis- specific transmembrane protein C11ORF94 plays a critical role in sperm-oocyte membrane binding by Hongying Hao, Baolu Shi, Jiacheng Zhang, Ao Dai, Wenhao Li, Haidi Chen, Wenya Ji, Chenjia Gong, Chang Zhang, Jing Li, Li Chen, Bin Yao, Peng Hu, Hao Yang, Juergen Brosius, Shanshan Lai, Qinghua Shi, Cheng Deng

“…In consistence, when the sperm of C11orf94-deficient mice were microinjected into the oocyte cytoplasm, fertilized oocytes were obtained and developed normally to blastocysts. Proteomics analysis revealed that C11orf94 influenced the expression of multiple gene products known to be indispensable for sperm-oocyte binding and fusion, including IZUMO1, EQTN and CRISP1. …”
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Donor template delivery by recombinant adeno-associated virus for the production of knock-in mice by Graham Duddy, Katherine Courtis, Juliette Horwood, Jessica Olsen, Helen Horsler, Tina Hodgson, Sunita Varsani-Brown, Abdullah Abdullah, Laura Denti, Hollie Lane, Fabio Delaqua, Julia Janzen, Molly Strom, Ian Rosewell, Katharine Crawley, Benjamin Davies

“…By comparing our production data against genetically altered knock-in mice generated via gene targeting in embryonic stem cells and their microinjection into blastocysts, we assess the animal cost of the two methods. …”
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Oxidative damage contributes to bisphenol S-induced development block at 2-cell stage preimplantation embryos in mice through inhibiting of embryonic genome activation by Anfeng Ning, Nansong Xiao, Hu Wang, Chunyi Guan, Xu Ma, Hongfei Xia

“…The results showed that 10–6 mol/L BPS exposure delayed the blastocysts stage, and exposure to 10–4 mol/L BPS induced 2-cell block in mice preimplantation embryos. …”
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SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo by Delphine Haouzi, Frida Entezami, Edward Tuaillon, Anna Gala, Alice Ferrières-Hoa, Sophie Brouillet, Alain R. Thierry, Samir Hamamah

“…The impact of COS protocols on endometrial gene expression profile of SARS-CoV-2-associated receptors and proteases is minimal, suggesting no additional potential risks during stimulated ART procedure. In blastocysts, <i>ACE2</i>, <i>BSG</i>, <i>CTSL</i>, <i>CTSA</i> and <i>FURIN</i> were detectable in the entire cohort at high expression level. …”
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Developmental competence of interspecies cloned embryos produced using cells from large Japanese field mice (Apodemus speciosus) and oocytes from laboratory mice (Mus musculus dome... by Rika AZUMA, Yuki HATANAKA, Seung-Wook SHIN, Hitoshi MURAI, Minoru MIYASHITA, Masayuki ANZAI, Kazuya MATSUMOTO

“…Only a small number of iSCNT embryos developed to the 4-cell (0–4%) and blastocysts (0–1%) stages under sequential treatment using trichostatin A (TSA) and vitamin C (VC) supplemented with deionized bovine serum albumin (d-BSA). …”
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Evaluation of Cardiovascular Toxicity of Folic Acid and 6S-5-Methyltetrahydrofolate-Calcium in Early Embryonic Development by Zenglin Lian, Zhuanbin Wu, Rui Gu, Yurong Wang, Chenhua Wu, Zhengpei Cheng, Mingfang He, Yanli Wang, Yongzhi Cheng, Harvest F. Gu

“…We then analyzed the differently expressed genes in in vitro fertilized murine blastocysts cultured with FA and MTHF-Ca. By using gene-expression profiling, we identified a novel gene in mice that encodes an essential eukaryotic translation initiation factor (<i>Eif1ad7</i>). …”
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#264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development by Chungha Lee, Sangho Lee, Taeseop Shin, Geon Kim, Jieun Do, Jaephil Do, Ji Hyang Kim, Kyoung Hee Choi, Jae Young Kim, YongKeun Park

“…Results: Using the proposed method, live mouse embryos were monitored at different developmental stages, ranging from 2-cell embryos to expanded blastocysts. The measured 3D RI maps of the developing embryos reveal 3D landscape of subcellular features inside the embryos (Fig. 1). …”
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A cost-effectiveness analysis of the use of highly purified human menopausal gonadotropins in assisted reproductive technology programs in gonadotropinreleasing hormone agonist or... by Ju. S. Drapkina, N. P. Makarova, V. Yu. Smolnikova, B. V. Zingerenko

“…Prescription of highly purified HMG (Menopur Multidose 1200 IU) to stimulate ovarian function in patients of different ages is accompanied by an adequate number of mature oocytes, good quality blastocysts, as well as satisfactory indicators of clinical pregnancy. …”
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Facts about Stem Cells and Importance of Them by Masumeh Saeidi, Ali Khakshour, Habibolah Taghizadeh Moghadam, Foad Faroughi, Majid Rahban

“…In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in various tissues. …”
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#248 : Maternal Hypercholesterolemia Would Increase the Incidence of Embryo Aneuploidy in Couples with Recurrent Implantation Failure by Yang Liu, Tianxiang Ni, Junhao Yan

“…Compared with the NonH group, SHC group had a significantly increased aneuploid embryo rate [48.3% vs. 36.7%, P=0.006; adjusted OR (95% CI) = 1.52(1.04-2.22)], as well as a reduced number of good-quality blastocysts [3.00±2.29 vs. 3.74±2.77, P=0.033]. The SHC group showed a lower cumulative live-birth rate (47.0% vs. 40.0%), good birth outcome (37.2% vs. 34.5%) and a higher risk of clinical pregnancy loss (11.1% vs. 17.9%) but did not reach statistical significance. …”
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Influence of different environments on oocyte maturation and development of bovine embryos in vitro by Vitalii Kovpak, Oksana Kovpak, Yelyzaveta Babii, Serhii Derkach, Yurii Masalovych

“…Thus, at 48 hours of cultivation (the initial stage of embryo development), 64.3 ± 1.0 and 60.3 ± 1.4% of 2-8 cell embryos were obtained, and on Day 8 – 25.3 ± 1.0 and 20.0 ± 0.6% of blastocysts, respectively. The results of a comparison of bovine embryo culture protocols showed that when using both Minitube and Origio media, the percentage of division and the percentage of resulting embryos corresponded to their known values. …”
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Efficient reprogramming of naïve-like induced pluripotent stem cells from porcine adipose-derived stem cells with a feeder-independent and serum-free system. by Yu Zhang, Chao Wei, Pengfei Zhang, Xia Li, Tong Liu, Yong Pu, Yunsheng Li, Zubing Cao, Hongguo Cao, Ya Liu, Xiaorong Zhang, Yunhai Zhang

“…Furthermore, nuclear transfer embryos from naïve-like piPSCs could develop to blastocysts with improved quality. Thus, we provided an efficient protocol for generating naïve-like piPSCs from pADSCs in a feeder-independent and serum-free system with controlled regulation of exogenous genes, which may facilitate optimization of culture media and the production of transgenic pigs.…”
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