Cholesterol and egg activation by Wang, Li

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Clinical application of next generation sequencing-based haplotype linkage analysis in the preimplantation genetic testing for germline mosaicisms by Dongjia Chen, Yan Xu, Yu Fu, Yali Wang, Yuliang Liu, Chenhui Ding, Bing Cai, Jiafu Pan, Jing Wang, Rong Li, Jing Guo, Han Zhang, Yanhong Zeng, Xiaoting Shen, Canquan Zhou

“…Trophectoderm biopsy and multiple displacement amplification (MDA) were performed for a total of nine blastocysts. NGS and Sanger sequencing were performed in genomic DNA of family members and embryonic MDA products to detect DMD deletion and TSC1 mutation, respectively. …”
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Xeno-free generation of new Yazd human embryonic stem cell lines (Yazd4-7) as a prior stage toward good manufacturing practice of clinical-grade raw materials from discarded embryo... by Fatemeh Hajizadeh-Tafti, Jalal Golzadeh, Fatemeh Akyash, Somayyeh-Sadat Tahajjodi, Ehsan Farashahi-Yazd, Hassan Heidarian-Meimandi, Behrouz Aflatoonian

“…In this study, we report the xeno-free derivation of new Yazd hESC lines (Yazd4-7), without using immunosurgery, by culturing intact zona-free blastocysts obtained from discarded embryos onto the YhFF#8 cells as a feeder layer in a microdrop culture system. …”
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Plastic used in in vitro fertilization procedures induces massive placental gene expression alterationsResearch in context by Franck Kouakou, Anne-Lyse Denizot, Audrey L'Hostis, Julie Colet, Sébastien Jacques, Amira Sallem, Ahmed Ziyyat, Daniel Vaiman, Jean-Philippe Wolf

“…Methods: Three groups of blastocysts were transferred to pseudopregnant mice. …”
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GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment by Jia Mao, Ying Feng, Yayun Zheng, Yaqiu Gao, Linyu Zhang, Xinrui Sun, Yilun Wu, Xiaofeng Zhu, Fang Ma

“…Methods The effects of G protein-coupled receptor 65 (GPR65) on cell adhesion and other cellular functions were tested using JAR spheroids, mouse blastocysts, and HTR-8/SVneo cells. Specifically, we employed HTR-8/SVneo cells for gene overexpression and silencing to investigate the underlying mechanism of GPR65's impact on trophoblast cell function under acidic conditions. …”
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Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development by Tatiana Kamaletdinova, Wen Zong, Pavel Urbánek, Sijia Wang, Mara Sannai, Paulius Grigaravičius, Wenli Sun, Zahra Fanaei-Kahrani, Aswin Mangerich, Michael O. Hottiger, Tangliang Li, Zhao-Qi Wang

“…Embryonic stem (ES) cell lines can be derived from PARP1^ΔC/ΔC blastocysts, and these mutant ES cells can differentiate into all three germ layers, yet, with a high degree of cystic structures, indicating defects in epithelial cells. …”
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Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome by Anastasia V. Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella I. Enukashvily

“…However, transcription of <i>OCT4</i> mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. …”
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Impact of dienogest pretreatment on IVF-ET outcomes in patients with endometriosis: a systematic review and meta-analysis by Wenjing Shao, Yuying Li, Yanli Wang

“…Secondary outcomes included retrieved oocytes, mature oocytes, blastocysts, growing follicles, transferrable embryos, fertilization rate, implantation rate, and miscarriage rate. …”
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Outcomes of assisted reproductive technologies in discordant couples with HIV infection in a woman by Helen V. Mituyrina, Svetlana G. Perminova, Elena S. Sannikova

“…In the main group, a significantly lower number of oocyte-cumulus complexes [6.5 (3; 12) and 9 (6; 15); p0.001], zygotes [4 (2; 8) and 6 (4; 10); p0.001] and blastocysts [2 (0; 2) and 4 (1; 6); p0.001] were obtained in comparison with the control group. …”
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A 3D “sandwich” co-culture system with vascular niche supports mouse embryo development from E3.5 to E7.5 in vitro by Junjun Xu, Linye Zhang, Zihui Ye, Binwen Chang, Zheng Tu, Xuguang Du, Xi Wen, Yili Teng

“…However, limitations remain regarding the in vitro culture platforms used before implanting mouse embryos and the normal development of mouse blastocysts in vitro. Furthermore, vascular niche support during mouse embryo development from embryonic day (E) 3.5 to E7.5 is unknown in vitro. …”
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Investigation of the role of X chromosome inactivation and androgen receptor CAG repeat polymorphisms in patients with recurrent pregnancy loss: a prospective case–control study by Yilun Sui, Jing Fu, Shuo Zhang, Lu Li, Xiaoxi Sun

“…AR polymorphisms were assessed using quantitative fluorescent polymerase chain reactions and direct sequencing. Blastocysts of the patients with RPL were tested by single nucleotide polymorphism microarray based preimplantation genetic testing for aneuploidy. …”
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Personalization of IVF-ICSI workflow based on patient characteristics improves IVF laboratory outcomes and embryo ploidy by PGT-A by Brandon A. Wyse, Noga Fuchs Weizman, Janice Montbriand, Rima Kharonsky, Ran Antes, Rina Abramov, Svetlana Madjunkova, Clifford L. Librach

“…The aim of this study was to examine outcomes following different workflow protocols in IVF-ICSI procedures in blastocysts that have undergone undisturbed incubation and preimplantation genetic testing for aneuploidy (PGT-A) prior to transfer. …”
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OGM and WES identifies translocation breakpoints in PKD1 gene in an polycystic kidney patient and healthy baby delivered using PGT by Peiwen Xu, Lijuan Wang, Jing Li, Sexin Huang, Ming Gao, Ranran Kang, Jie Li, Hongqiang Xie, Xiaowei Liu, Junhao Yan, Xuan Gao, Yuan Gao

“…Based on this molecular diagnosis, a non-carrier embryo was selected out from three blastocysts. With preimplantation genetic testing (PGT) after in vitro fertilization (IVF), it was then transferred into uterus. …”
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Rare nonclassic type of Congenital adrenal hyperplasia due to 21-hydroxylase deficiency and genotype-phenotypic correlation by Yanru Hou, Yian Li, Jiajia Ai, Li Tian

“…Furthermore, PGTM gene detection was carried out on the four blastocysts of the proband’s offspring through IVF. …”
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Loss of Renewal of Extracellular Vesicles: Harmful Effects on Embryo Development in vitro by Qu P, Zhao J, Hu H, Cao W, Zhang Y, Qi J, Meng B, Zhao J, Liu S, Ding C, Wu Y, Liu E

“…RNA-seq, reactive oxygen species (ROS) staining, immunofluorescence of LC3 and H3K36me3, and qPCR of oxidative stress-related genes and autophagy-related genes of blastocysts in the in vivo group, non-EVs group, con-EVs group, and R-EVsM group was implemented.Results: Incubation of embryos with 9.1× 1011 EV particles/mL had a positive effect at 48 h and 72 h, which disappeared by 96 h, however. …”
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Non-invasive preimplantation genetic testing (niPGT): the next revolution in reproductive genetics? by Leaver, M, Wells, D

“…The recent discovery of DNA within the blastocoele fluid (BF) of blastocysts and in spent embryo culture media (SCM) has led to interest in the development of non-invasive methods of PGT (niPGT).…”

Knockdown of Placental Major Facilitator Superfamily Domain Containing 2a in Pregnant Mice Reduces Fetal Brain Growth and Phospholipid Docosahexaenoic Acid Content by Theresa L. Powell, Kenneth Barentsen, Owen Vaughan, Charis Uhlson, Karin Zemski Berry, Kathryn Erickson, Kelsey Faer, Stephanie S. Chassen, Thomas Jansson

“…We hypothesized that placenta-specific knockdown of MFSD2a in pregnant mice reduces phospholipid DHA accumulation in the fetal brain. Methods: Mouse blastocysts (E3.5) were transduced with an EGFP-expressing lentivirus containing either an shRNA targeting MFSD2a or a non-coding sequence (SCR), then transferred to pseudopregnant females. …”
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<i>Tropheryma whipplei</i>, <i>Helicobacter pylori</i>, and Intestinal Protozoal Co-Infections in Italian and Immigrant Populations: A Cross-Sectional Study by Lucia Moro, Elena Pomari, Martina Leonardi, Giulia La Marca, Barbara Pajola, Cristina Mazzi, Chiara Piubelli, Anna Beltrame

“…In addition, the proportion of Blastocysts positive finding was also significantly higher in HP-infected than in non-infected (40.9% vs. 17.4%, <i>p</i> = 0.018). …”
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Novel mutations in PLCZ1 lead to early embryonic arrest as a male factor by Yunying Lin, Yunying Lin, Yi Huang, Yi Huang, Boyu Li, Boyu Li, Ting Zhang, Ting Zhang, Yichao Niu, Yichao Niu, Shuanggang Hu, Shuanggang Hu, Ying Ding, Ying Ding, Guangxin Yao, Guangxin Yao, Zhe Wei, Zhe Wei, Ning Yao, Ning Yao, Yejie Yao, Yejie Yao, Yao Lu, Yao Lu, Yaqiong He, Yaqiong He, Qinling Zhu, Qinling Zhu, Ling Zhang, Ling Zhang, Yun Sun, Yun Sun

“…However, these rescued zygotes showed a lower potential in developing into blastocysts when cultured in vitro. Thus, fresh cleavage transfer was tried and two live births were successfully achieved thereafter. …”
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Application of machine learning to predict aneuploidy and mosaicism in embryos from in vitro fertilization cyclesAJOG Global Reports at a Glance by José A. Ortiz, PhD, Ruth Morales, PhD, Belén Lledó, PhD, Juan A. Vicente, PhD, Julio González, PhD, Eva M. García-Hernández, PhD, Alba Cascales, MSc, Jorge Ten, PhD, Andrea Bernabeu, PhD, MD, Rafael Bernabeu, PhD, MD

“…The trophoectoderm biopsies on day-5, -6, or -7 blastocysts were analyzed by preimplantation genetic testing for aneuploidies (PGT-A). …”
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