Granulocyte colony-stimulating factor priming improves embryos and pregnancy rate in patients with poor ovarian reserve: a randomized controlled trial by Masao Jinno, Yukoku Tamaoka, Koji Teruya, Aiko Watanabe, Naohisa Hatakeyama, Tomoya Goda, Hayato Kimata, Yuichi Jinno

“…With priming, significantly more patients achieved cryopreservation of redundant blastocysts. The cumulative live birth rate was 32% in 50 patients with priming, significantly higher than 14% in 49 controls (relative risk, 2.8; 95% confidence interval, 1.04–7.7). …”
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Antioxidant Activity and Anti-Apoptotic Effect of the Small Molecule Procyanidin B1 in Early Mouse Embryonic Development Produced by Somatic Cell Nuclear Transfer by Wei Gao, Tingting Yu, Guomeng Li, Wei Shu, Yongxun Jin, Mingjun Zhang, Xianfeng Yu

“…DNA damage can lead to cell apoptosis; therefore, we also tested the level of apoptosis of blastocysts, and we found that PB1 reduced the level of apoptosis. …”
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The development of new biomarkers of spermatozoa quality in cattle by Lindsey Fallon, Edgar Diaz-Miranda, Edgar Diaz-Miranda, Lauren Hamilton, Peter Sutovsky, Peter Sutovsky, Peter Sutovsky, Michal Zigo, Thomas E. Spencer, M. Sofia Ortega

“…This study utilized imaging techniques to identify potential biomarkers of sperm quality, using sires previously classified as high (n = 4) or low (n = 4) performing at producing blastocysts in vitro. Spermatozoa were assessed before and following a gradient purification protocol, to understand how populations of cells are impacted by such protocols and may differ between in vivo and in vitro use. …”
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Murine pluripotent stem cells that escape differentiation inside teratomas maintain pluripotency by Yangli Pei, Liang Yue, Wei Zhang, Jinzhu Xiang, Zhu Ma, Jianyong Han

“…OCT4-MES (mouse embryonic stem cells) were isolated from the blastocysts of 3.5-day OCT4-EGFP mice (transgenic mice express EGFP cDNA under the control of the Pou5f1 promoter) embryos, and TG iPS 1-7 (induced pluripotent stem cells) were generated from mouse embryonic fibroblasts (MEFs) from 13.5-day OCT4-EGFP mice embryos by infecting them with a virus carrying OCT4, SOX2, KLF4 and c-MYC. …”
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Influence of the starting day of luteal phase stimulation on double stimulation cycles by Ana Fuentes, Cristina García-Ajofrín, Ruth Romero, Juan Carlos Castillo, Juan Carlos Castillo, Jose A. Ortíz, Mónica Hortal, Jaime Guerrero, Andrea Bernabeu, Andrea Bernabeu, Rafael Bernabeu, Rafael Bernabeu

“…A lower number of fertilized oocytes was observed when stimulation began before the fourth day (3.36 ± 2.80 vs. 3.95 ± 2.53 vs. 4.03 ± 2.73 vs. 4.48 ± 3.11; p=0,036). The number of blastocysts was higher when the stimulation started 5-6 days after retrieval (1.82 ± 1.74 vs. 2.13 ± 1.61 vs. 2.33 ± 2.06 vs. 2.91 ± 2.39; p= 0,030).DiscussionThe number of oocytes retrieved does not differ depending on the day that stimulation begins. …”
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The cumulative live birth rate of recombinant follicle-stimulating hormone alfa verse urinary human follicle-stimulating hormone for ovarian stimulation in assisted reproductive te... by Chunxia Yang, Naijun Dong, Feng Li, Yurong Ji, Yu Pan, Hong She

“…Conclusions rFSH-alfa and uFSH have similar pregnancy rates and LBR per transfer cycle, rFSH-alfa might achieve more transferrable blastocysts and higher CLBR per started cycle compared to uFSH.…”
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Deep sequencing analysis of the developing mouse brain reveals a novel microRNA. by Ling, King Hwa, Brautigan, Peter J., Hahn, Christopher N., Daish, Tasman, Rayner, John R., Cheah, Pike See, Raison, Joy M., Piltz, Sandra, Mann, Jeffrey R., Mattiske, Deidre M., Thomas, Paul Q., Adelson, David L., Scott, Hamish S.

“…Mm_br_e15_1181 biogenesis was Dicer1-dependent and was expressed in E3.5 blastocysts and E7 whole embryos. Embryo-wide expression patterns were observed at E9.5 and E11.5 followed by a near complete loss of expression by E13.5, with expression restricted to a specialised layer of cells within the developing and early postnatal brain. …”
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Metabolites Secreted by Bovine Embryos In Vitro Predict Pregnancies That the Recipient Plasma Metabolome Cannot, and Vice Versa by Enrique Gomez, Nuria Canela, Pol Herrero, Adrià Cereto, Isabel Gimeno, Susana Carrocera, David Martin-Gonzalez, Antonio Murillo, Marta Muñoz

“…Day-6 in vitro produced embryos developed singly in modified synthetic oviduct fluid culture medium (CM) drops for 24 h were vitrified as Day-7 blastocysts and transferred to recipients. Day-0 and Day-7 recipient plasma (<i>N</i> = 36 × 2) and CM (<i>N</i> = 36) were analyzed by gas chromatography coupled to the quadrupole time of flight mass spectrometry (GC-qTOF). …”
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Occurrence and Genetic Diversity of Protist Parasites in Captive Non-Human Primates, Zookeepers, and Free-Living Sympatric Rats in the Córdoba Zoo Conservation Centre, Southern Spa... by Pamela C. Köster, Alejandro Dashti, Begoña Bailo, Aly S. Muadica, Jenny G. Maloney, Mónica Santín, Carmen Chicharro, Silvia Migueláñez, Francisco J. Nieto, David Cano-Terriza, Ignacio García-Bocanegra, Rafael Guerra, Francisco Ponce-Gordo, Rafael Calero-Bernal, David González-Barrio, David Carmena

“…Zoonotic transmission of <i>Blastocysts</i> ST1 was highly suspected between captive NHP and zookeepers.…”
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Could the Age Difference of a Single Calendar Year between Patients Undergoing IVF at 34, 35 or at 36 Years Old Affect the IVF Outcome? A Retrospective Data Analysis by Konstantinos Pantos, Konstantinos Sfakianoudis, Sokratis Grigoriadis, Evangelos Maziotis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Anastasios Atzampos, Sevasti Koulouraki, Michael Koutsilieris, Nikolaos Vlahos, George Mastorakos, Mara Simopoulou

“…Embryo transfer including two blastocysts was performed for every patient. Comparisons were performed regarding hormonal profile, response to stimulation, quality of transferred embryos, positive hCG test and clinical pregnancy rate. …”
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Outcomes of a GnRH Agonist Trigger Following a GnRH Antagonist or Flexible Progestin-Primed Ovarian Stimulation Cycle by Erkan Kalafat, Erkan Kalafat, Engin Turkgeldi, Sule Yıldız, Merve Dizdar, Ipek Keles, Baris Ata, Baris Ata

“…However, the number of remaining blastocysts was higher in the fPPOS group (median: 5.0 vs. 6.0, P &lt; 0.001). …”
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Anti-blastocystis activity of hop extracts in vitro by S.I. Pokhil, V.V. Kazmirchuk, O.M. Tymchenko, V.Y. Yevsiukova, A.L. Melnyk

“…To detect the anti­blastocystic activity hop extracts and metronidazole were tested in the range from 1000 μg/ml to 1 μg/ml.The presence and number of viable Blastocystis sp. cells were determined after 24, 48, 72 and 96 hours. …”
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Serum Progesterone Profile Across the Mid and Late Luteal Phase in Artificial Cycles Is Associated With Pregnancy Outcome by Elena Labarta, Elena Labarta, Cristina Rodríguez-Varela, Giulia Mariani, Ernesto Bosch, Ernesto Bosch

“…Eligible patients were aged between 18-42 years, with a normal uterus, and being transferred 1-2 good quality blastocysts in a frozen ET cycle after an artificial endometrial preparation with estradiol valerate and vaginal micronized progesterone (400 mg/12 hours).ResultsA total of 127 patients were included. …”
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Influence of sire fertility status on conceptus-induced transcriptomic response of the bovine endometrium by E. O’Callaghan, J.M. Sánchez, M.B. Rabaglino, M. McDonald, H. Liu, T.E. Spencer, S. Fair, D.A. Kenny, P. Lonergan

“…To generate elongated conceptuses, Day 7 blastocysts produced in vitro using frozen-thawed sperm from Holstein Friesian bulls (3 High fertility, HF and 3 Low fertility, LF) were transferred in groups of 5–10 into synchronized heifers (n = 7 heifers per bull) and recovered following slaughter on Day 15. …”
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The role of serum vitamin D in patients with normal ovarian reserve undergoing the first IVF/ICSI cycle by Rong Luo, Jiahui Wang, Yu Yang, Cen Xu, Minyan Yang, Dandan Zhu, Jia Wang, Ping Zhang, Hongshan Ge

“…The primary outcome was clinical pregnancy, while the secondary outcomes were mature oocytes, oocyte fertilization, available cleavage embryos, available blastocysts, biochemical pregnancy, early abortion, and embryo implantation. …”
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Impact of Chorionic Somatomammotropin In Vivo RNA Interference Phenotype on Uteroplacental Expression of the IGF Axis by Taylor K. Hord, Amelia R. Tanner, Victoria C. Kennedy, Cameron S. Lynch, Quinton A. Winger, Paul J. Rozance, Russell V. Anthony

“…The trophectoderm of hatched blastocysts (9 days of gestation, dGA) were infected with a lentivirus expressing either a non-targeting sequence (NTS RNAi) control or CSH-specific shRNA (CSH RNAi) prior to embryo transfer into synchronized recipient ewes. …”
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Stem Cells Era in Maxillofacial Surgery by Amr Mohamed Bayoumi

“…</p><p style="text-align: justify;">There are two basic types of stem cells, depending on their origin:</p><p style="text-align: justify;">• Embryonic Stem Cells (ESCs): ESCs are initiated by a fetus that has been fertilized in an in vitro fertilization clinic (100,200 organized cell blastocysts). They are called a pluripotent cell population and are characterized by the ability to maintain the karyotype. …”
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Supplementary dydrogesterone is beneficial as luteal phase support in artificial frozen-thawed embryo transfer cycles compared to micronized progesterone alone by Angela Vidal, Carolin Dhakal, Nathalie Werth, Jürgen Michael Weiss, Dirk Lehnick, Alexandra Sabrina Kohl Schwartz, Alexandra Sabrina Kohl Schwartz

“…The proportions of blastocysts and single transferred embryos were lower in the micronized progesterone gel group. …”
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DEVELOPMENT OF MURINE STEM CELLS WITH CONDITIONAL KNOCKOUT OF HUMANIZED SNCA GENE by E. A. Patrakhanov, V. M. Pokrovsky, A. Yu. Karagodina, A. M. Krayushkina, N. S. Zhunusov, A. V. Deykin, M. V. Korokin, M. V. Pokrovsky, O. B. Altukhova

“…The accumulated data indicate that pathogenic mutations in the Snca gene are associated with the development of neurodegenerative brain damage, indicating the relevance of studying the synuclein neurobiological role.The aim of the study was to create a genetically modified clone of mouse stem cells with a conditional knockout of humanized α-synuclein, which can be used for the reinjection into mouse blastocysts, as well as for basic and applied in vitro research in the field of pathophysiology and neuropharmacology.Materials and methods. …”
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Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization by Giacomo Lazzarino, Romina Pallisco, Gabriele Bilotta, Ilaria Listorti, Renata Mangione, Miriam Wissam Saab, Giuseppe Caruso, Angela Maria Amorini, Maria Violetta Brundo, Giuseppe Lazzarino, Barbara Tavazzi, Pasquale Bilotta

“…Furthermore, Biomarker Score values correlated with outcome measures of ovarian stimulation, in vitro fertilization, number and quality of blastocysts, clinical pregnancy, and healthy offspring. …”
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