Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method by Osamu Shibata, Kenya Kamimura, Yuto Tanaka, Kohei Ogawa, Takashi Owaki, Chiyumi Oda, Shinichi Morita, Atsushi Kimura, Hiroyuki Abe, Satoshi Ikarashi, Kazunao Hayashi, Takeshi Yokoo, Shuji Terai

“…KRASG12D and YAP combined transfer contributes to the E- to N-cadherin switch in pancreatic ductal adenocarcinoma cells and to tumor metastases. …”
Get full text

Premna puberula root petroleum ether extract inhibits proliferation, migration and invasion, and induces apoptosis through mitochondrial pathway in non-small cell lung cancer A549... by Nian Yang, Xiaoyan Jia, Yao Yang, Jingming Niu, Xia Wu, Furong Ding, Minyi Tian, Dongxin Tang

“…It prevented the migration and invasion of A549 cells by reducing the expression of MMP-2 and N-cadherin. In conclusion, P. puberula root PEE can be a new source of antitumor agents and has significant anticancer activity in vitro.…”
Get full text

Transforming growth factor-β1 and SMAD signalling pathway in the small airways of smokers and patients with COPD: potential role in driving fibrotic type-2 epithelial mesenchymal t... by Samuel James Brake, Wenying Lu, Wenying Lu, Collin Chia, Collin Chia, Greg Haug, Josie Larby, Ashutosh Hardikar, Gurpreet K. Singhera, Gurpreet K. Singhera, Tillie L. Hackett, Tillie L. Hackett, Mathew Suji Eapen, Sukhwinder Singh Sohal, Sukhwinder Singh Sohal

“…Tissue was also stained for EMT markers E-cadherin, S100A4 and vimentin.ResultsThe Staining of pSMAD2/3 was significantly increased in the epithelium, and RBM of all COPD groups compared to NC (p <0.0005). …”
Get full text

A mutation that blocks integrin α4β7 activation prevents adaptive immune-mediated colitis without increasing susceptibility to innate colitis by Hailong Zhang, Yajuan Zheng, Youdong Pan, Changdong Lin, Shihui Wang, Zhanjun Yan, Ling Lu, Gaoxiang Ge, Jinsong Li, Yi Arial Zeng, Jianfeng Chen

“…By contrast, integrin αEβ7 mediates the adhesion of lymphocytes to gut epithelial cells by interacting with E-cadherin. Integrin β7 blocking antibodies have shown efficacy in clinical management of inflammatory bowel disease (IBD); however, fully blocking β7 function leads to the depletion of colonic regulatory T (Treg) cells and exacerbates dextran sulfate sodium (DSS)-induced colitis by evoking aberrant innate immunity, implying its potential adverse effect for IBD management. …”
Get full text

ACE2 Attenuates Epithelial-Mesenchymal Transition in MLE-12 Cells Induced by Silica by Li S, Li Y, Xu H, Wei Z, Yang Y, Jin F, Zhang M, Wang C, Song W, Huo J, Zhao J, Yang X, Yang F

“…The mRNA expression of ACE and ACE2, and protein expression of the renin-angiotensin system (RAS) components and EMT indicators were studied by qRT-PCR and Western blot, respectively.Results: DIZE treatment and overexpression of ACE2 markedly inhibited the formation of silica-induced lung fibrosis and increased the level of E-cadherin, with concomitant downregulation of pro-collagen, vimentin, and α-SMA via RAS signaling. …”
Get full text

Diesel exhaust particle exposure exacerbates ciliary and epithelial barrier dysfunction in the multiciliated bronchial epithelium models by Eunsook Park, Bu-Yeo Kim, Seahyoung Lee, Kuk Hui Son, Jihye Bang, Se Hyang Hong, Joong Won Lee, Kyung-Ok Uhm, Hyun-Jeong Kwak, Hyun Joung Lim

“…Furthermore, DEPs significantly increased the expression of MUC5AC, whereas they decreased the expression of epithelial junction proteins, namely, ZO1, Occludin, and E-cadherin. Impairment of mucociliary clearance by DEPs significantly improved the release of epithelial-derived inflammatory and fibrotic mediators such as IL-1β, IL-6, IL-8, GM-CSF, MMP-1, VEGF, and S100A9. …”
Get full text

Expression of SOCS1 and CXCL12 Proteins in Primary Breast Cancer Are Associated with Presence of Circulating Tumor Cells in Peripheral Blood by Bozena Smolkova, Michal Mego, Viera Horvathova Kajabova, Zuzana Cierna, Ludovit Danihel, Tatiana Sedlackova, Gabriel Minarik, Iveta Zmetakova, Tomas Krivulcik, Paulina Gronesova, Marian Karaba, Juraj Benca, Daniel Pindak, Jozef Mardiak, James M. Reuben, Ivana Fridrichova

“…Expression of APC, ADAM23, CXCL12, E-cadherin, RASSF1, SYK, TIMP3, BRMS1, and SOCS1 proteins in primary breast tumor tissue was evaluated by immunohistochemistry. …”
Get full text

Molecular Heterogeneity of High Grade Colorectal Adenocarcinoma by Cristian Perna, Antonia Navarro, Ignacio Ruz-Caracuel, Tamara Caniego-Casas, Eva Cristóbal, Susanna Leskelä, Federico Longo, Alejandra Caminoa, Almudena Santón, Reyes Ferreiro, David Pizarro, María Luisa Palacios-Berraquero, José Palacios

“…The expression of MLH1, PMS2, MSH2, MSH6, p53, E-cadherin, CDX2, CK20, CD8, PDL1, PAN-TRK, c-MET, SMARCB1, ARID1A, SMARCA2, and SMARCA4 was analyzed by immunohistochemistry. …”
Get full text

Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells by Sheng-Kai Huang, Ruo-Xuan Ni, Wen-Jie Wang, Wen-Jie Wang, Di Wang, Mei Zhao, Cheng-Zhi Lei, Xiao-Jie Sun, Chang-Zhi Huang, Ping Bai, Yi-Qun Che, Jian-Ping Xu

“…Western blotting showed that cell cycle-related proteins cyclin A2 and cyclin E and interstitial-associated proteins Snail and N-cadherin were upregulated and p53 signaling pathway-related proteins were downregulated in LINC00673-overexpressing HeLa and SiHa cells.ConclusionLINC00673 plays an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.…”
Get full text

Developing a new animal model of subcutaneous transplanted prostate cancer with cell sheet technology by ZHOU Shukui, ZHANG Dongliang, WANG Xiang, LIU Lei, LI Zeng, YANG Shengke, LIAO Hong

“…The DU145 cell sheet was rich in collagen fibrin with high vimentin expression and low E-cadherin expression. H-E staining showed that the tumor structure was dense with obvious infiltration of surrounding muscles in the cell sheet group, while the tumor had more vacuoles and a clear boundary with surrounding tissues in the cell suspension group 4 weeks after subcutaneous transplantation. …”
Get full text

Cdh5-mediated Fpn1 deletion exerts neuroprotective effects during the acute phase and inhibitory effects during the recovery phase of ischemic stroke by Huiwen Zheng, Xin Guo, Shaomeng Kang, Zhongda Li, Tian Tian, Jianhua Li, Fudi Wang, Peng Yu, Shiyang Chang, Yan-zhong Chang

“…Herein, we conditionally deleted Fpn1 in mouse endothelial cells (ECs), using VE-cadherin-Cre transgenic mice, and explored the impact on brain iron homeostasis after stroke. …”
Get full text

Histopathological and Immunohistochemical Prognostic Factors in High-Grade Non-Endometrioid Carcinomas of the Endometrium (HG-NECs): Is It Possible to Identify Subgroups at Increas... by Michele Paudice, Chiara Maria Biatta, Giulia Scaglione, Alessia Parodi, Serafina Mammoliti, Melita Moioli, Maria Grazia Centurioni, Fabio Barra, Simone Ferrero, Franco De Cian, Katia Mazzocco, Valerio Gaetano Vellone

“…Methods: Each surgical specimen was routinely processed; the most significant block was selected for immunohistochemistry and tested for ER, PR, ki67, p53, E-cadherin, β-catenin, Bcl-2 and cyclin D1. For each immunomarker, the percentage of positive tumor cells was evaluated (%) and dichotomized as low and high according to the distribution in the study population. …”
Get full text

Genomic and epigenomic basis of breast invasive lobular carcinomas lacking CDH1 genetic alterations by Higinio Dopeso, Andrea M. Gazzo, Fatemeh Derakhshan, David N. Brown, Pier Selenica, Sahar Jalali, Arnaud Da Cruz Paula, Antonio Marra, Edaise M. da Silva, Thais Basili, Laxmi Gusain, Lorraine Colon-Cartagena, Shirin Issa Bhaloo, Hunter Green, Chad Vanderbilt, Steffi Oesterreich, Anne Grabenstetter, M. Gabriela Kuba, Dara Ross, Dilip Giri, Hannah Y. Wen, Hong Zhang, Edi Brogi, Britta Weigelt, Fresia Pareja, Jorge S. Reis-Filho

“…Abstract CDH1 (E-cadherin) bi-allelic inactivation is the hallmark alteration of breast invasive lobular carcinoma (ILC), resulting in its discohesive phenotype. …”
Get full text

Euphorbia factor L1 inhibited transport channel and energy metabolism in human colon adenocarcinoma cell line Caco-2 by Xiaoying Chen, Hong Hu, Xiaohuang Lin, Mengting Chen, Wenqiang Bao, Yajiao Wu, Chutao Li, Yadong Gao, Shaozhang Hou, Qiaomei Yang, Li Chen, Jian Zhang, Kunqi Chen, Qi Wang, An Zhu

“…The interaction between EFL1 and the tight junction proteins Occludin, Claudin-4, Tricellulin, ZO-1, JAM-1, and E-cadherin was simulated by molecular docking. The expression of proteins involved in the energy metabolism, the ion transporters and aquaporins, the tight junction, and the F-actin cytoskeleton were detected by Western blotting and cell immunofluorescence. …”
Get full text

Effect of SARS-CoV-2 proteins on vascular permeability by Rossana Rauti, Meishar Shahoha, Yael Leichtmann-Bardoogo, Rami Nasser, Eyal Paz, Rina Tamir, Victoria Miller, Tal Babich, Kfir Shaked, Avner Ehrlich, Konstantinos Ioannidis, Yaakov Nahmias, Roded Sharan, Uri Ashery, Ben Meir Maoz

“…While validating the PPI network model, we found that the tight junction (TJ) proteins cadherin-5, ZO-1, and β-catenin are affected by nsp2, nsp5_c145a, and nsp7 consistent with the model prediction. …”
Get full text

Meta-Analysis of the Luminal and Basal Subtypes of Bladder Cancer and the Identification of Signature Immunohistochemical Markers for Clinical Use by Vipulkumar Dadhania, Miao Zhang, Li Zhang, Jolanta Bondaruk, Tadeusz Majewski, Arlene Siefker-Radtke, Charles C. Guo, Colin Dinney, David E. Cogdell, Shizhen Zhang, Sangkyou Lee, June G. Lee, John N. Weinstein, Keith Baggerly, David McConkey, Bogdan Czerniak

“…In addition, luminal tumors were characterized by the overexpression of E-Cadherin, HER2/3, Rab-25, and Src. Basal tumors showed the expression signature similar to the basal layer of normal urothelium. …”
Get full text

Ethanolic extract of Origanum syriacum L. leaves exhibits potent anti-breast cancer potential and robust antioxidant properties by Joelle Mesmar, Rola Abdallah, Kamar Hamade, Serine Baydoun, Najlaa Al-Thani, Abdullah Shaito, Marc Maresca, Adnan Badran, Elias Baydoun

“…Protein levels of markers of apoptosis (BCL-2, pro-Caspase3, p53), proliferation (p21, Ki67), cell migration, invasion, or adhesion (FAK, E-cadherin), angiogenesis (iNOS), and cell signaling (STAT3, p38) were determined by immunoblotting. …”
Get full text

Basement membrane product, endostatin, as a link between inflammation, coagulation and vascular permeability in COVID-19 and non-COVID-19 acute respiratory distress syndrome by Katharina Jandl, Katharina Jandl, Johannes Lorenz Berg, Johannes Lorenz Berg, Anna Birnhuber, Anna Birnhuber, Elisabeth Fliesser, Izabela Borek, Benjamin Seeliger, Sascha David, Julius J. Schmidt, Gregor Gorkiewicz, Martin Zacharias, Tobias Welte, Horst Olschewski, Horst Olschewski, Akos Heinemann, Malgorzata Wygrecka, Malgorzata Wygrecka, Grazyna Kwapiszewska, Grazyna Kwapiszewska, Grazyna Kwapiszewska

“…Finally, we showed a positive correlation of endostatin with soluble disease markers VE-Cadherin, c-reactive protein (CRP), fibrinogen, and interleukin (IL)-6 in our COVID-19 cohort.ConclusionThe cumulative effects of endostatin on propagating neutrophil chemotaxis, platelet aggregation, and endothelial cell barrier disruption may suggest endostatin as a link between those cellular events in ARDS pathology.…”
Get full text

Comparison of renal histopathology and gene expression profiles between severe COVID-19 and bacterial sepsis in critically ill patients by Meint Volbeda, Daniela Jou-Valencia, Marius C. van den Heuvel, Marjolein Knoester, Peter J. Zwiers, Janesh Pillay, Stefan P. Berger, Peter H. J. van der Voort, Jan G. Zijlstra, Matijs van Meurs, Jill Moser

“…Renal mRNA levels of endothelial integrity markers CD31, PV-1 and VE-Cadherin did not differ from control individuals yet were increased in bacterial sepsis patients (CD31 fold change 2.3, p = 0.0006, PV-1 fold change 1.5, p = 0.008). …”
Get full text

Fibroblast growth factors preserve blood–brain barrier integrity through RhoA inhibition after intracerebral hemorrhage in mice by Bin Huang, Paul R. Krafft, Qingyi Ma, William B. Rolland, Basak Caner, Tim Lekic, Anatol Manaenko, Mai Le, Jiping Tang, John H. Zhang

“…Evans blue extravasation as well as Western blot analysis for the quantification of activated FGFR, Akt, Ras-related C3 botulinum toxin substrate 1 (Rac1), Ras homolog gene family member A (RhoA) and adherens junction proteins (p120-catenin, β-catenin and VE-cadherin) were conducted at 72 h post-cICH. FGF treatment reduced perihematomal brain edema and improved neurofunctional deficits at 72 h after experimental ICH (p<0.05, compared to vehicle); however, FGFR and PI3K inhibition reversed these neuroprotective effects. …”
Get full text