Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein. by Ken-ichiro Nakajima, Kanako Yokoyama, Taichi Koizumi, Ayako Koizumi, Tomiko Asakura, Tohru Terada, Katsuyoshi Masuda, Keisuke Ito, Akiko Shimizu-Ibuka, Takumi Misaka, Keiko Abe

“…To identify the critical histidine residue(s) responsible for this activity, we produced a series of His-to-Ala neoculin variants and evaluated their sweetness levels using cell-based calcium imaging and a human sensory test. Our results suggest that NBS His11 functions as a primary pH sensor for neoculin to elicit taste modification. …”
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Massive normalization of olfactory bulb output in mice with a 'monoclonal nose' by Roland, Benjamin, Jordan, Rebecca, Sosulski, Dara L., Diodato, Assunta, Fukunaga, Izumi, Wickersham, Ian R., Franks, Kevin M., Schaefer, Andreas T., Fleischmann, Alexander

“…To gain insights into how olfactory circuits can process such degraded inputs we characterized odor-evoked responses of olfactory bulb mitral cells and interneurons. Surprisingly, calcium imaging experiments reveal that mitral cell responses in M71 transgenic mice are largely normal, highlighting a remarkable capacity of olfactory circuits to normalize sensory input. …”
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Distinct prefrontal top-down circuits differentially modulate sensorimotor behavior by Huda, Rafiq, Sipe, Grayson O, Breton-Provencher, Vincent, Cruz, K Guadalupe, Pho, Gerald N, Adam, Elie, Gunter, Liadan M, Sullins, Austin, Wickersham, Ian R, Sur, Mriganka

“…Projection-specific two-photon calcium imaging and optogenetic manipulations show that ACC outputs to the superior colliculus, a key midbrain structure for response selection, principally coordinate specific motor responses. …”
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Widespread and Highly Correlated Somato-dendritic Activity in Cortical Layer 5 Neurons by Beaulieu-Laroche, Lou, Toloza, Enrique HS, Brown, Norma J, Harnett, Mark T

“…Beaulieu-Laroche et al. perform near-simultaneous calcium imaging of somatic and dendritic activity to reveal that active dendritic integration is an integral feature of information processing in cortical pyramidal neurons.…”
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Neural Regression, Representational Similarity, Model Zoology Neural Taskonomy at Scale in Rodent Visual Cortex by Conwell, Colin, Mayo, David, Buice, Michael A., Katz, Boris, Alvarez, George A., Barbu, Andrei

“…Using the Allen Brain Observatory’s 2-photon calcium-imaging dataset of activity in over 6,000 reliable rodent visual cortical neurons recorded in response to natural scenes, we replicate previous findings and resolve previous discrepancies, ultimately demonstrating that modern neural networks can in fact be used to explain activity in the mouse visual cortex to a more reasonable degree than previously suggested. …”
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Technological Innovation and Integration of Whole Brain Imaging, Olfactory Stimulation, and Correlative Microscopy in Larval Zebrafish by Swain, Corban N.

“…And, finally, we showcase a set of proof-of-concept experiments and analyses which demonstrate our ability to integrate olfactory stimulation, whole-brain calcium imaging, behavioral recording, and structural staining in individual larva.…”
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Functional Clustering Drives Encoding Improvement in a Developing Brain Network during Awake Visual Learning by Dunfield, Derek, Podgorski, Kaspar, Haas, Kurt

“…Using two-photon calcium imaging to monitor evoked activity in over 100 neurons simultaneously, we investigate network-level changes in the developing Xenopus laevis tectum during visual training with motion stimuli. …”
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An Overview of Bayesian Methods for Neural Spike Train Analysis by Chen, Zhe

“…On the application side, the topics include spike sorting, tuning curve estimation, neural encoding and decoding, deconvolution of spike trains from calcium imaging signals, and inference of neuronal functional connectivity and synchrony. …”
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Of sounds, photons and maps: in vivo optical characterisation of the auditory thalamocortical system by Vasquez Lopez, SA

“…To better understand the rules governing thalamocortical connectivity and the origin of cortical maps, I used in vivo two-photon calcium imaging to characterise the properties of thalamic axons innervating different layers and subfields of mouse auditory cortex. …”

Developmental Axon Stretch Stimulates Neuron Growth While Maintaining Normal Electrical Activity, Intracellular Calcium Flux, and Somatic Morphology by Joseph R Loverde, Joseph R Loverde, Bryan J Pfister

“…Unlike traumatic axonal injury, functional calcium imaging of the soma revealed no shifts in free intracellular calcium during axon stretch. …”
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Molecular Mechanism Analysis of STIM1 Thermal Sensation by Xiaoling Liu, Tianyuan Zheng, Yan Jiang, Lei Wang, Yuchen Zhang, Qiyu Liang, Yuejie Chen

“…In this study, a variety of mutants of STIM1, Orai1 and Orai3 were generated. The single–cell calcium imaging and confocal analysis were used to evaluate the thermal sensitivity of the resulting STIM mutants and the interaction between STIM1 and Orai mutants in response to temperature. …”
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In Vivo Transplantation of Enteric Neural Crest Cells into Mouse Gut; Engraftment, Functional Integration and Long-Term Safety. by Julie E Cooper, Conor J McCann, Dipa Natarajan, Shanas Choudhury, Werend Boesmans, Jean-Marie Delalande, Pieter Vanden Berghe, Alan J Burns, Nikhil Thapar

“…Intestines were then assessed for ENCC integration and differentiation using immunohistochemistry, cell function using calcium imaging, and long-term safety using PCR to detect off-target YFP expression.YFP+ ENCCs engrafted, proliferated and differentiated into enteric neurons and glia within recipient ganglionic gut. …”
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A Molecular and Cellular Context-Dependent Role for Ir76b in Detection of Amino Acid Taste by Anindya Ganguly, Lisa Pang, Vi-Khoi Duong, Angelina Lee, Hanni Schoniger, Erika Varady, Anupama Dahanukar

“…From a Drosophila RNAi screen, we identify an ionotropic receptor, Ir76b, as necessary for yeast preference. Using calcium imaging, we identify Ir76b+ amino acid taste neurons in legs, overlapping partially with sweet neurons but not those that sense other tastants. …”
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Reelin secreted by GABAergic neurons regulates glutamate receptor homeostasis. by Cecilia Gonzalez Campo, Mélanie Sinagra, Danièle Verrier, Olivier J Manzoni, Pascale Chavis

“…We demonstrate that secreted reelin binds to receptors of the lipoprotein family on neurons with a punctate reelin IR. Secondly, using calcium imaging techniques, we examined the physiological consequences of reelin secretion blockade. …”
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In vivo chromatic and spatial tuning of foveolar retinal ganglion cells in Macaca fascicularis. by Tyler Godat, Nicolas P Cottaris, Sara Patterson, Kendall Kohout, Keith Parkins, Qiang Yang, Jennifer M Strazzeri, Juliette E McGregor, David H Brainard, William H Merigan, David R Williams

“…Here we overcome this challenge by combining high resolution fluorescence adaptive optics ophthalmoscopy with calcium imaging to optically record functional responses of foveal retinal ganglion cells in the living eye. …”
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Puerarin Enhances Ca2+ Reuptake and Ca2+ Content of Sarcoplasmic Reticulum in Murine Embryonic Stem Cell-Derived Cardiomyocytes via Upregulation of SERCA2a by Lu Wang, Yurong Cui, Qinghua Liu, Yuanlong Song, Qinghua Hu, Ming Tang, Jürgen Hescheler, Jiaoya Xi

“…Methods: Murine ES-CMs were harvested by embryoid body-based differentiation method. Confocal calcium imaging and whole-cell patch clamps were performed to assess the function of SR. …”
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Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo by Attila Kaszas, Gergely Szalay, Andrea Slézia, Alexandra Bojdán, Ivo Vanzetta, Balázs Hangya, Balázs Rózsa, Rodney O’Connor, David Moreau

“…Using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f, here we show that the application of infrared neural stimulation induces intracellular calcium signals in Layer 2/3 neurons in mouse cortex in vivo. …”
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Antipsychotic drugs selectively decorrelate long-range interactions in deep cortical layers by Matthias Heindorf, Georg B Keller

“…We then used widefield calcium imaging to determine the cell type-specific functional effects of antipsychotic drugs in mouse dorsal cortex under different conditions of visuomotor coupling. …”
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Neuropeptide VF neurons promote sleep via the serotonergic raphe by Daniel A Lee, Grigorios Oikonomou, Tasha Cammidge, Andrey Andreev, Young Hong, Hannah Hurley, David A Prober

“…Using genetic labeling and calcium imaging, we show that npvf-expressing neurons innervate and can activate serotonergic RN neurons. …”
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L-Type Calcium Channel Inhibition Contributes to the Proarrhythmic Effects of Aconitine in Human Cardiomyocytes. by Jianjun Wu, Xiangchong Wang, Ying Ying Chung, Cai Hong Koh, Zhenfeng Liu, Huicai Guo, Qiang Yuan, Chuan Wang, Suwen Su, Heming Wei

“…Laser-scanning confocal calcium imaging analysis showed that ACO decreased the duration and amplitude of [Ca2+]i transients and increased in the beating frequencies by over 60%. …”
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