Glucose tightly controls morphological and functional properties of astrocytes by Chun-Yao eLee, Glenn eDallérac, Pascal eEzan, Miroslava eAnderova, Miroslava eAnderova, Nathalie eRouach

“…We indeed found that glucoprivation decreases their gap junction-mediated coupling, while it progressively and reversibly increases their intracellular calcium levels during the slow depression of synaptic transmission occurring simultaneously, as assessed by dual electrophysiological and calcium imaging recordings. Together, these data indicate that astrocytes rapidly respond to metabolic dysfunctions, and are therefore central to the neuroglial dialogue at play in brain adaptation to glycopenia.…”
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DRG-Derived Neural Progenitors Differentiate into Functional Enteric Neurons Following Transplantation in the Postnatal Colon by Hui Hu, Yuanyuan Ding, Wenbo Mu, Ying Li, Yanpeng Wang, Weifang Jiang, Yong Fu, Jinfa Tou, Wei Chen

“…The exogenous EGFP+ neurons were physiologically functional as shown by the activity of calcium imaging. This study shows that that other tissues besides the postnatal bowel harbor neural crest stem cells or neural progenitors that have the potential to differentiate into functional enteric neurons in vivo and can potentially be used for intestinal nerve regeneration. …”
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Mycobacteria attenuate nociceptive responses by formyl peptide receptor triggered opioid peptide release from neutrophils. by Heike L Rittner, Dagmar Hackel, Philipp Voigt, Shaaban Mousa, Andrea Stolz, Dominika Labuz, Michael Schäfer, Michael Schaefer, Christoph Stein, Alexander Brack

“…Mycobacterium butyricum induced a rise in intracellular calcium as determined by FURA loading and calcium imaging. Opioid peptide release was blocked by intracellular calcium chelation as well as phosphoinositol-3-kinase inhibition. …”
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Mitochondrial Reactive Oxygen Species Mediate Activation of TRPV1 and Calcium Entry Following Peripheral Sensory Axotomy by Bradley Kievit, Aaron D. Johnstone, Julien Gibon, Philip A. Barker

“…We coupled transections with intra-axonal calcium imaging and found that Ca2+ influx is sharply reduced in axons lacking trpv1 (for transient receptor potential cation channel vanilloid 1) and in axons treated with capsazepine (CPZ), a TRPV1 antagonist. …”
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Fast and slow feedforward inhibitory circuits for cortical odor processing by Norimitsu Suzuki, Malinda LS Tantirigama, K Phyu Aung, Helena HY Huang, John M Bekkers

“…Using in vivo two-photon-targeted patch clamping and calcium imaging in mice, we find that odors evoke strong excitation in two classes of interneurons – neurogliaform (NG) cells and horizontal (HZ) cells – that provide feedforward inhibition in layer 1 of the piriform cortex. …”
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Neural Systems Under Change of Scale by Erik D. Fagerholm, W. M. C. Foulkes, Yasir Gallero-Salas, Yasir Gallero-Salas, Fritjof Helmchen, Fritjof Helmchen, Karl J. Friston, Robert Leech, Rosalyn J. Moran

“…As an example of such a system, we use calcium imaging collected in murine cortex and show that the dynamical critical exponent, as defined in renormalization group theory, can be estimated in an empirical biological setting. …”
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Cholinergic Signals in Mouse Barrel Cortex during Active Whisker Sensing by Emmanuel Eggermann, Yves Kremer, Sylvain Crochet, Carl C.H. Petersen

“…However, a whisking-related cortical state change persists after thalamic inactivation, which is mediated at least in part by acetylcholine, as we show here by using whole-cell recordings, local pharmacology, axonal calcium imaging, and optogenetic stimulation. During whisking, we find prominent cholinergic signals in the barrel cortex, which suppress spontaneous cortical activity. …”
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Texture coarseness responsive neurons and their mapping in layer 2–3 of the rat barrel cortex in vivo by Liora Garion, Uri Dubin, Yoav Rubin, Mohamed Khateb, Yitzhak Schiller, Rony Azouz, Jackie Schiller

“…Using in vivo two-photon calcium imaging in the rat barrel cortex during artificial whisking against different surface coarseness or controlled passive whisker vibrations simulating different coarseness, we show that layer 2–3 neurons within barrel boundaries differentially respond to specific texture coarsenesses, while only a minority of neurons responded monotonically with increased or decreased surface coarseness. …”
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Laser-Induced Nuclear Damage Signaling and Communication in Astrocyte Networks Through Parp-Dependent Calcium Oscillations by Nicole M. Wakida, Ryan D. Ha, Edward K. Kim, Xiangduo Kong, Kyoko Yokomori, Michael W. Berns, Michael W. Berns

“…Combining laser nanosurgery with calcium imaging of primary astrocytes allow for spatial and temporal observation of the astrocyte network in response to nuclear damage. …”
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Global actions of nicotine on the striatal microcircuit by Victor E Plata, Mariana eDuhne, Jesús Esteban Pérez-Ortega, Ricardo eHernandez-Martinez, Pavel Ernesto Rueda Orozco, Elvira eGalarraga, Rene eDrucker-Colin, Jose eBargas

“…To answer this question some type of multicellular recording has to be used without losing single cell resolution. Here, we used calcium imaging and nicotine. It is known that in the presence of low micromolar N-Methyl-D-aspartate (NMDA), the striatal microcircuit exhibits neuronal activity consisting in the spontaneous synchronization of different neuron pools that interchange their activity following determined sequences. …”
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Cholinergic activity reflects reward expectations and predicts behavioral responses by Panna Hegedüs, Katalin Sviatkó, Bálint Király, Sergio Martínez-Bellver, Balázs Hangya

“…Therefore, we performed bulk calcium imaging and recorded spiking of identified cholinergic neurons from the basal forebrain of mice performing a probabilistic Pavlovian cued outcome task. …”
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Unveiling the functional connectivity of astrocytic networks with AstroNet, a graph reconstruction algorithm coupled to image processing by L. Zonca, F. C. Bellier, G. Milior, P. Aymard, J. Visser, A. Rancillac, N. Rouach, D. Holcman

“…To address this, we developed AstroNet, a data-driven algorithm that uses two-photon calcium imaging to map temporal correlations in astrocyte activation. …”
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Chemogenetic stimulation of the Gi pathway in astrocytes suppresses neuroinflammation by Jae‐Hong Kim, Md Habibur Rahman, Won Ha Lee, Kyoungho Suk

“…Similarly, in vitro calcium imaging showed that activation of the astrocytic Gi pathway attenuated intracellular calcium transients triggered by LPS treatment, suggesting a positive correlation between enhanced calcium transients and the inflammatory phenotype of astrocytes observed in the inflamed brain. …”
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Involvement of orexin neurons in fasting- and central adenosine-induced hypothermia by Takahiro Futatsuki, Akira Yamashita, Khairunnisa Novita Ikbar, Akihiro Yamanaka, Kazunori Arita, Yasuyuki Kakihana, Tomoyuki Kuwaki

“…Orexin neurons were active just before onset of hypothermia and during the recovery period as revealed by calcium imaging in vivo using G-CaMP. We next examined adenosine-induced hypothermia via an intracerebroventricular administration of an adenosine A1 receptor agonist, N6-cyclohexyladenosine (CHA), which induced hypothermia in both ORX-AB and WT mice. …”
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Visual projection neurons in the Drosophila lobula link feature detection to distinct behavioral programs by Ming Wu, Aljoscha Nern, W Ryan Williamson, Mai M Morimoto, Michael B Reiser, Gwyneth M Card, Gerald M Rubin

“…In vivo two-photon calcium imaging reveals that these LC types respond to looming stimuli, while another type does not, but instead responds to the motion of a small object. …”
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Programmable illumination and high-speed, multi-wavelength, confocal microscopy using a digital micromirror. by Franck P Martial, Nicholas A Hartell

“…We demonstrate the use of this system for high-speed calcium imaging using both a single wavelength calcium indicator and a genetically encoded, ratiometric, calcium sensor.…”
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Patterns of Spontaneous Local Network Activity in Developing Cerebral Cortex: Relationship to Adult Cognitive Function. by Alejandro Peinado, Charles K Abrams

“…To test this we used calcium imaging in brain slices and analyzed variations in the frequency and intensity of this early activity in one area, the entorhinal cortex (EC), in order to correlate early activity with level of cognitive function later in life. …”
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Impact of Magnetite Nanowires on In Vitro Hippocampal Neural Networks by Belén Cortés-Llanos, Rossana Rauti, Ángel Ayuso-Sacido, Lucas Pérez, Laura Ballerini

“…Hippocampal cultures were seeded on NWs devices, and after 14 days, the cell morphology was studied by immunocytochemistry and confocal microscopy. Live calcium imaging was performed to study neuronal activity. …”
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Extracellular Histones Activate Plasma Membrane Toll-Like Receptor 9 to Trigger Calcium Oscillations in Rat Pancreatic Acinar Tumor Cell AR4-2J by Hai Yan Guo, Zong Jie Cui

“…We have examined histone modulation of rat pancreatic acini and pancreatic acinar tumor cell AR4-2J by calcium imaging. Histones were found to have no effect on calcium in pancreatic acini but blocked calcium oscillations induced by cholecystokinin or acetylcholine. …”
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ER stress and subsequent activated calpain play a pivotal role in skeletal muscle wasting after severe burn injury. by Li Ma, Wanli Chu, Jiake Chai, Chuanan Shen, Dawei Li, Xiaoteng Wang

“…Tibial anterior muscle was harvested for transmission electron microscopy, calcium imaging, gene expression and protein analysis of ER stress / ubiquitin-proteasome system / autophagy, and calpain activity measurement. …”
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