Sperm Affects Head Sensory Neuron in Temperature Tolerance of Caenorhabditis elegans by Satoru Sonoda, Akane Ohta, Ayana Maruo, Tomoyo Ujisawa, Atsushi Kuhara

“…Genetic impairment of sperm caused abnormal cold tolerance, which was unexpectedly restored by impairment of temperature signaling in ASJ neurons. Calcium imaging revealed that ASJ neuronal activity in response to temperature was decreased in sperm mutant gsp-4 with impaired protein phosphatase 1 and rescued by expressing gsp-4 in sperm. …”
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Endothelin increases the proliferation of rat olfactory mucosa cells by Bertrand Bryche, Audrey Saint-Albin, Claire Le Poupon Schlegel, Christine Baly, Patrice Congar, Nicolas Meunier

“…We then used combined approaches of calcium imaging, reverse transcriptase-quantitative polymerase chain reaction and protein level measurements to show that endothelin was locally synthetized by the primary culture until it reached confluency. …”
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Modulation of sleep-courtship balance by nutritional status in Drosophila by José M Duhart, Victoria Baccini, Yanan Zhang, Daniel R Machado, Kyunghee Koh

“…Trans-synaptic tracing and calcium imaging identified dopaminergic neurons projecting to the protocerebral bridge (DA-PB) as postsynaptic partners of P1 neurons. …”
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Two-Photon Voltage Imaging of Spontaneous Activity from Multiple Neurons Reveals Network Activity in Brain Tissue by Binglun Li, Mariya Chavarha, Yuho Kobayashi, Satoshi Yoshinaga, Kazunori Nakajima, Michael Z. Lin, Takafumi Inoue

“…We provide evidence for the developmental changes in intralaminar horizontal connections in somatosensory cortex layer 2/3 with a greater sensitivity than calcium imaging. This method thus enables investigation of neuronal network connectivity at the cellular resolution in brain tissue.…”
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β-amyloid monomer scavenging by an anticalin protein prevents neuronal hyperactivity in mouse models of Alzheimer’s Disease by Benedikt Zott, Lea Nästle, Christine Grienberger, Felix Unger, Manuel M. Knauer, Christian Wolf, Aylin Keskin-Dargin, Anna Feuerbach, Marc Aurel Busche, Arne Skerra, Arthur Konnerth

“…Using in vivo two-photon calcium imaging and SF-iGluSnFR-based glutamate imaging in hippocampal slices, we demonstrate that an Aβ binding anticalin protein (Aβ-anticalin) can suppress early neuronal hyperactivity and synaptic glutamate accumulation in the APP23xPS45 mouse model of β-amyloidosis. …”
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A Hallucinogenic Serotonin-2A Receptor Agonist Reduces Visual Response Gain and Alters Temporal Dynamics in Mouse V1 by Angie M. Michaiel, Philip R.L. Parker, Cristopher M. Niell

“…Using widefield and two-photon calcium imaging and single-unit electrophysiology in awake mice, we find that administration of the hallucinogenic selective 5-HT2AR agonist DOI (2,5-dimethoxy-4-iodoamphetamine) leads to a net reduction in visual response amplitude and surround suppression in primary visual cortex, as well as disrupted temporal dynamics. …”
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The role of pruriceptors in enhancing sensitivity to pruritogens in a murine chronic compression model of dorsal root ganglion by Tao Wang, Jin Tao, Yehong Fang, Chao Ma

“…In addition, we investigated if pruritogen-evoked activities of DRG neurons were enhanced in this model. In vivo calcium imaging revealed that compressed DRG neurons exhibited enhanced responses to histamine and BAM8-22. …”
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Hydrogen sulfide suppresses outward rectifier potassium currents in human pluripotent stem cell-derived cardiomyocytes. by Heming Wei, Guangqin Zhang, Suhua Qiu, Jun Lu, Jingwei Sheng, Manasi, Grace Tan, Philip Wong, Shu Uin Gan, Winston Shim

“…The effect of NaHS, a donor of H₂S, on action potential (AP), outward rectifier potassium currents (I(Ks) and I(Kr)), L-type Ca²⁺ currents (I(CaL)) and hyperpolarization-activated inward current (I(f)) were determined by patch-clamp electrophysiology and confocal calcium imaging. In a concentration-dependent manner, NaHS (100 to 300 µM) consistently altered the action potential properties including prolonging action potential duration (APD) and slowing down contracting rates of ventricular-and atrial-like cardiomyocytes derived from both hESCs and hiPSCs. …”
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Neocortical inhibitory imbalance predicts successful sensory detection by Christopher A. Deister, Alexander I. Moore, Jakob Voigts, Sophia Bechek, Rebecca Lichtin, Tyler C. Brown, Christopher I. Moore

“…We addressed this with population calcium imaging of FS/PVs and putative PYR neurons during threshold detection. …”
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In vivo chromatic and spatial tuning of foveolar retinal ganglion cells in Macaca fascicularis by Tyler Godat, Nicolas P. Cottaris, Sara Patterson, Kendall Kohout, Keith Parkins, Qiang Yang, Jennifer M. Strazzeri, Juliette E. McGregor, David H. Brainard, William H. Merigan, David R. Williams

“…Here we overcome this challenge by combining high resolution fluorescence adaptive optics ophthalmoscopy with calcium imaging to optically record functional responses of foveal retinal ganglion cells in the living eye. …”
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Red ginseng extract blocks histamine-dependent itch by inhibition of H1R/TRPV1 pathway in sensory neurons by Yongwoo Jang, Wook-Joo Lee, Gyu-Sang Hong, Won-Sik Shim

“…Methods: To examine the antipruritic effect of RGE, we performed in vivo scratching behavior test in mice, as well as in vitro calcium imaging and whole-cell patch clamp experiments to elucidate underlying molecular mechanisms. …”
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Specific Ion Channels Control Sensory Gain, Sensitivity, and Kinetics in a Tonic Thermonociceptor by Gabriella Saro, Andrei-Stefan Lia, Saurabh Thapliyal, Filipe Marques, Karl Emanuel Busch, Dominique A. Glauser

“…Here, we investigate the function of a single type of genetically identified C. elegans thermonociceptor named FLP. Using calcium imaging in vivo, we demonstrate that FLP encodes thermal information in a tonic and graded manner over a wide thermal range spanning from noxious cold to noxious heat (8°C–36°C). …”
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Long-range inhibition from prelimbic to cingulate areas of the medial prefrontal cortex enhances network activity and response execution by Nao Utashiro, Duncan Archibald Allan MacLaren, Yu-Chao Liu, Kaneschka Yaqubi, Birgit Wojak, Hannah Monyer

“…In vitro electrophysiological and in vivo calcium imaging studies support the notion that the GABAergic projection neurons from the PrL to the Cg1/2 exert a crucial role in regulating the activity in the target area by disinhibiting layer 5 output neurons. …”
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NMDA receptor inhibition increases, synchronizes, and stabilizes the collective pancreatic beta cell activity: Insights through multilayer network analysis. by Marko Šterk, Lidija Križančić Bombek, Maša Skelin Klemen, Marjan Slak Rupnik, Marko Marhl, Andraž Stožer, Marko Gosak

“…To address this issue, we combined confocal functional multicellular calcium imaging in mouse tissue slices with network science approaches. …”
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Erasable hippocampal neural signatures predict memory discrimination by Nathaniel R. Kinsky, Daniel J. Orlin, Evan A. Ruesch, Brian Kim, Siria Coello, Kamran Diba, Steve Ramirez

“…Summary: Memories involving the hippocampus can take several days to consolidate, challenging efforts to uncover the neuronal signatures underlying this process. Here, we use calcium imaging in freely moving mice to track the hippocampal dynamics underlying memory consolidation across a 10-day contextual fear conditioning task. …”
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Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes. by Anna Llach, Cristina E Molina, Enrique Alvarez-Lacalle, Lluis Tort, Raul Benítez, Leif Hove-Madsen

“…Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. …”
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Experimental and Computational Methods for the Study of Cerebral Organoids: A Review by Daniele Poli, Chiara Magliaro, Arti Ahluwalia, Arti Ahluwalia

“…Well-established techniques based on current/voltage clamp, optogenetics, calcium imaging, and Micro-Electrode Arrays (MEAs) are proposed for monitoring intra- and extra-cellular responses underlying neuronal dynamics and functional connections. …”
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GABA(A) receptors containing the α2 subunit are critical for direction-selective inhibition in the retina. by Olivia Nicola Auferkorte, Tom Baden, Sanjeev Kumar Kaushalya, Nawal Zabouri, Uwe Rudolph, Silke Haverkamp, Thomas Euler

“…In GABA(A)R α2 knock-out (KO) mice, light responses of retinal GCs recorded with two-photon calcium imaging revealed a significant impairment of DS responses compared to their wild-type littermates. …”
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C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples by Enrico Lanza, Martina Di Rocco, Silvia Schwartz, Davide Caprini, Edoardo Milanetti, Giuseppe Ferrarese, Maria Teresa Lonardo, Luca Pannone, Giancarlo Ruocco, Simone Martinelli, Viola Folli

“…Behavioral assays on animals lacking AWC sensory neurons demonstrate the relevance of these neurons in sensing cancer odorants: calcium imaging on AWC increases the accuracy of the discrimination (97.22%). …”
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The cellular basis of mechanosensation in mammalian tongue by Yalda Moayedi, Shan Xu, Sophie K. Obayashi, Benjamin U. Hoffman, Gregory J. Gerling, Ellen A. Lumpkin

“…Summary: Mechanosensory neurons that innervate the tongue provide essential information to guide feeding, speech, and social grooming. We use in vivo calcium imaging of mouse trigeminal ganglion neurons to identify functional groups of mechanosensory neurons innervating the anterior tongue. …”
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