Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations by Evgeny S. Gerasimov, Dmitry A. Afonin, Oksana A. Korzhavina, Julius Lukeš, Ross Low, Neil Hall, Kevin Tyler, Vyacheslav Yurchenko, Sara L. Zimmer

“…We utilized computational methods to show that the T. borreli transcriptome includes a substantial number of transcripts with inconsistent editing patterns, apparently products of non-canonical editing. This species utilizes the most extensive uridine deletion compared to other studied kinetoplastids to enforce amino acid conservation of cryptogene products, although insertions still remain more frequent. …”
Get full text

Essential Control of the Function of the Striatopallidal Neuron by Pre-coupled Complexes of Adenosine A2A-Dopamine D2 Receptor Heterotetramers and Adenylyl Cyclase by Sergi Ferré, Jordi Bonaventura, Wendy Zhu, Candice Hatcher-Solis, Jaume Taura, Jaume Taura, César Quiroz, Ning-Sheng Cai, Estefanía Moreno, Verónica Casadó-Anguera, Alexxai V. Kravitz, Kimberly R. Thompson, Dardo G. Tomasi, Gemma Navarro, Arnau Cordomí, Leonardo Pardo, Carme Lluís, Carmen W. Dessauer, Nora D. Volkow, Vicent Casadó, Francisco Ciruela, Francisco Ciruela, Diomedes E. Logothetis, Daniel Zwilling

“…The A2AR-D2R heteromer has a tetrameric structure, with two homodimers, which allows not only multiple allosteric interactions between different orthosteric ligands, agonists, and antagonists, but also the canonical Gs-Gi antagonistic interaction at the level of AC5. …”
Get full text

Dysregulated Immunological Functionome and Dysfunctional Metabolic Pathway Recognized for the Pathogenesis of Borderline Ovarian Tumors by Integrative Polygenic Analytics by Chia-Ming Chang, Yao-Feng Li, Hsin-Chung Lin, Kai-Hsi Lu, Tzu-Wei Lin, Li-Chun Liu, Kuo-Min Su, Cheng-Chang Chang

“…We also noticed that a dysfunctional pathway of galactose catabolism had taken place among all BOTs, SBOTs, and MBOTs from the analyzed gene set databases of canonical pathways. With the help of immunostaining, we verified significantly higher performance of interleukin 6 (IL6) and galactose-1-phosphate uridylyltransferase (GALT) among BOTs than the controls. …”
Get full text

Involvement of extracellular vesicles in the progression, diagnosis, treatment, and prevention of whole-body ionizing radiation-induced immune dysfunction by Roland F. Seim, Laura E. Herring, Angie L. Mordant, Micah L. Willis, Micah L. Willis, Shannon M. Wallet, Leon G. Coleman, Leon G. Coleman, Robert Maile

“…WBIR-EVs (9 Gy) were biologically active and blunted immune responses to LPS in RAW264.7 macrophages, inhibiting canonical signaling pathways associated with wound healing and phagosome formation. …”
Get full text

Transcriptomic signatures reveal a shift towards an anti-inflammatory gene expression profile but also the induction of type I and type II interferon signaling networks through ary... by Johannes R. Schmidt, Johannes R. Schmidt, Janine Haupt, Janine Haupt, Sina Riemschneider, Christoph Kämpf, Dennis Löffler, Dennis Löffler, Conny Blumert, Conny Blumert, Kristin Reiche, Kristin Reiche, Ulrike Koehl, Ulrike Koehl, Stefan Kalkhof, Stefan Kalkhof, Stefan Kalkhof, Jörg Lehmann, Jörg Lehmann

“…Mapping of known aryl hydrocarbon response element (AHRE) sequence motifs in identified DEGs revealed more than 200 genes not possessing any AHRE, and therefore being not eligible for canonical regulation. Bioinformatic approaches modeled a central role of type I and type II interferons in the regulation of those genes. …”
Get full text

Identification of immunological patterns characterizing immune-related psoriasis reactions in oncological patients in therapy with anti-PD-1 checkpoint inhibitors by Martina Morelli, Maria Luigia Carbone, Giovanni Luca Scaglione, Claudia Scarponi, Valentina Di Francesco, Sabatino Pallotta, Federica De Galitiis, Siavash Rahimi, Stefania Madonna, Cristina Maria Failla, Cristina Albanesi

“…The pathogenesis of anti-PD-1-induced psoriasis has yet to be clarified, even though it is plausible that some innate and adaptive immunity processes are in common with canonical psoriasis. The genetic predisposition to psoriasis of patients could also be a contributing factor. …”
Get full text

Six NSCL/P Loci Show Associations With Normal-Range Craniofacial Variation by Karlijne Indencleef, Karlijne Indencleef, Jasmien Roosenboom, Hanne Hoskens, Hanne Hoskens, Julie D. White, Mark D. Shriver, Stephen Richmond, Hilde Peeters, Eleanor Feingold, Mary L. Marazita, Mary L. Marazita, John R. Shaffer, John R. Shaffer, Seth M. Weinberg, Seth M. Weinberg, Greet Hens, Peter Claes, Peter Claes

“…The effect of each SNP was tested using a multivariate canonical correlation analysis (CCA) against 63 hierarchically-constructed facial segments in each of the three datasets and meta-analyzed. …”
Get full text

Honduras HIV cohort: HLA class I and CCR5-Δ32 profiles and their associations with HIV disease outcome by Humberto Valenzuela-Ponce, Candy Carbajal, Maribel Soto-Nava, Daniela Tapia-Trejo, Claudia García-Morales, Wendy Murillo, Ivette Lorenzana, Gustavo Reyes-Terán, Santiago Ávila-Ríos

“…IMPORTANCE We identify both canonical and novel human leukocyte antigen (HLA)-HIV associations, providing a first step toward improved understanding of HIV immune control among the understudied Honduras Mestizo population. …”
Get full text

Clinical Impact of Molecular Subtyping of Pancreatic Cancer by Xu Zhou, Xu Zhou, Kai Hu, Kai Hu, Peter Bailey, Peter Bailey, Peter Bailey, Christoph Springfeld, Susanne Roth, Roma Kurilov, Benedikt Brors, Benedikt Brors, Thomas Gress, Malte Buchholz, Jingyu An, Jingyu An, Kongyuan Wei, Kongyuan Wei, Teresa Peccerella, Teresa Peccerella, Markus W. Büchler, Thilo Hackert, John P. Neoptolemos, John P. Neoptolemos

“…There is, however, a molecular diversity beyond mutations in genes responsible for producing classical canonical signaling pathways. Pancreatic cancer is almost unique in promoting an excess production of other components of the stroma, resulting in a complex tumor microenvironment that contributes to tumor development, progression, and response to treatment. …”
Get full text

A low-dimensional cognitive-network space in Alzheimer’s disease and frontotemporal dementia by Lorenzo Pini, Siemon C de Lange, Francesca Benedetta Pizzini, Ilaria Boscolo Galazzo, Rosa Manenti, Maria Cotelli, Samantha Galluzzi, Maria Sofia Cotelli, Maurizio Corbetta, Martijn P van den Heuvel, Michela Pievani

“…We confirmed these results by means of a multivariate analysis (canonical correlation). Conclusions A low-dimensional representation can account for a large variance in cognitive scores in the continuum from normal to pathological aging. …”
Get full text

Oncogenic Alterations in Histologically Negative Lymph Nodes Are Associated with Prognosis of Patients with Stage I Lung Adenocarcinoma by Yiping Tian, Qian Lai, Yuansi Zheng, Lisha Ying, Canming Wang, Jiaoyue Jin, Minran Huang, Yingxue Wu, Huizhang Li, Jianjun Zhang, Dan Su

“…<i>Results</i>: A total of 102 (82.9%) patients had at least one canonical oncogenic alteration detected in primary tumors, and 57 LNs from 12 patients (11.8%) were found to carry the identical oncogenic alterations detected in the corresponding primary tumor tissues, including <i>EGFR</i> mutations (six cases), <i>KRAS</i> mutations (three cases), <i>ALK</i> fusion (one case), <i>BRAF</i> mutation (one case) and <i>HER2</i> & <i>NRAS</i> co-mutations (one case). …”
Get full text

Single‐cell RNA sequencing reveals characteristics of myeloid cells in post-acute sequelae of SARS-CoV-2 patients with persistent respiratory symptoms by Hyundong Yoon, Logan S. Dean, Logan S. Dean, Boonyanudh Jiyarom, Vedbar S. Khadka, Youping Deng, Vivek R. Nerurkar, Dominic C. Chow, Dominic C. Chow, Cecilia M. Shikuma, Cecilia M. Shikuma, Cecilia M. Shikuma, Gehan Devendra, Gehan Devendra, Youngil Koh, Youngil Koh, Juwon Park, Juwon Park

“…After integrating scRNA-seq data with a naïve participant from a published dataset, 11 distinct cell populations were identified based on the expression of canonical markers. The proportion of myeloid-lineage cells ([MLCs]; CD14+/CD16+monocytes, and dendritic cells) was increased in PPASC (n=2) compared to controls (n=2). …”
Get full text

Significant but partial lipoprotein lipase functional loss caused by a novel occurrence of rare LPL biallelic variants by Yuepeng Hu, Jian-Min Chen, Han Zuo, Na Pu, Guofu Zhang, Yichen Duan, Gang Li, Zhihui Tong, Weiqin Li, Baiqiang Li, Qi Yang

“…The aim was to identify rare variants, including nonsense, frameshift, missense, small in-frame deletions or insertions, and canonical splice site mutations. The functional impact of identified LPL missense variants on protein expression, secretion, and activity was assessed in HEK293T cells through single and co-transfection experiments, with and without heparin treatment. …”
Get full text

Flow Cytometry-Detected Immunological Markers and on Farm Recorded Parameters in Composite Cow Milk as Related to Udder Health Status by Giovanna De Matteis, Francesco Grandoni, Maria Carmela Scatà, Gennaro Catillo, Bianca Moioli, Luca Buttazzoni

“…Linear discriminant analysis (LDA) indicated that 5 of the 10 parameters could best be used to reveal the differences between positive and negative samples among the considered groups of SCCs. Furthermore, the Canonical discriminant analysis (CDA) indicated that composite milk samples with different SCC and infection status were clearly separated from each other in a two-dimensional space. …”
Get full text

Inhibitory to non-inhibitory evolution of the ζ subunit of the F1FO-ATPase of Paracoccus denitrificans and α-proteobacteria as related to mitochondrial endosymbiosis by Francisco Mendoza-Hoffmann, Francisco Mendoza-Hoffmann, Lingyun Yang, Damiano Buratto, Jorge Brito-Sánchez, Gilberto Garduño-Javier, Emiliano Salinas-López, Cristina Uribe-Álvarez, Raquel Ortega, Oliver Sotelo-Serrano, Miguel Ángel Cevallos, Leticia Ramírez-Silva, Salvador Uribe-Carvajal, Gerardo Pérez-Hernández, Heliodoro Celis-Sandoval, José J. García-Trejo

“…Introduction: The ζ subunit is a potent inhibitor of the F1FO-ATPase of Paracoccus denitrificans (PdF1FO-ATPase) and related α-proteobacteria different from the other two canonical inhibitors of bacterial (ε) and mitochondrial (IF1) F1FO-ATPases. ζ mimics mitochondrial IF1 in its inhibitory N-terminus, blocking the PdF1FO-ATPase activity as a unidirectional pawl-ratchet and allowing the PdF1FO-ATP synthase turnover. ζ is essential for the respiratory growth of P. denitrificans, as we showed by a Δζ knockout. …”
Get full text

Moda y salud en personas mayores. Revisión bibliográfica enfermera. by Mª Carmen Otero López, María Ballesteros Mantecón, Mª Milagros García Álvarez, Antonia Otero López, Sonia Pérez Martín, Mª del Carmen San Raimundo Morín, Casandra García Otero, Josefa González Centeno

“…Hablar de moda en la tercera edad, rompiendo los cánones de belleza preestablecidos, es extraño, la “arruga no es bella”, sin embargo, como consumidores, son un grupo activo, y se convertirán en el motor de la llamada silver economy, ya que “Son personas activas a las que les gusta cuidarse, hacer deporte, comer bien, ir a la moda y divertirse”. …”
Get full text

Perivascular localized cells commit erythropoiesis in PDGF‐B‐expressing solid tumors by Kayoko Hosaka, Chenchen Wang, Shiyue Zhang, Xue Lv, Takahiro Seki, Yin Zhang, Xu Jing, Jieyu Wu, Qiqiao Du, Xingkang He, Yulong Fan, Xuan Li, Makoto Kondo, Masahito Yoshihara, Hong Qian, Lihong Shi, Ping Zhu, Yuanfu Xu, Yunlong Yang, Tao Cheng, Yihai Cao

“…Single‐cell sequencing and colony formation assays validated the fact that, upon PDGF‐B stimulation, NG2+ cells isolated from tumors acted as erythroblast progenitor cells, which were distinctive from the canonical BM hematopoietic stem cells. Conclusions Our data provide a new concept of hematopoiesis within tumor tissues and novel mechanistic insights into perivascular localized cell‐derived erythroid cells within TME. …”
Get full text

Ecology, genetic diversity, and population structure among commercial varieties and local landraces of Capsicum spp. grown in northeastern states of India by Veerendra Kumar Verma, Avinash Pandey, Anbazhagan Thirugnanavel, Heiplanmi Rymbai, Niharika Dutta, Amit Kumar, Tshering Lhamu Bhutia, Anjani Kumar Jha, Vinay Kumar Mishra

“….); and cool to warm (11.3°C–33.1°C) and humid for bird’s eye chilli (C. frutescens L.) during the crop period. The canonical correspondence analysis has shown the significant impact of temperature on the agro-morphological traits and distribution of the landraces in their niche areas. …”
Get full text

Circulating Extracellular Vesicles and Particles Derived From Adipocytes: The Potential Role in Spreading MicroRNAs Associated With Cellular Senescence by Ionara Rodrigues Siqueira, Ionara Rodrigues Siqueira, Ionara Rodrigues Siqueira, Andressa de Souza Rodrigues, Marina Siqueira Flores, Eduarda Letícia Vieira Cunha, Madeleine Goldberg, Brennan Harmon, Rachael Batabyal, Robert J. Freishtat, Laura Reck Cechinel, Laura Reck Cechinel, Laura Reck Cechinel

“…A conservative filter was applied, and 18 microRNAs had experimentally validated and highly conserved predicted mRNA targets, with a total of 2,228 mRNAs. Canonical pathways, disease and functions, and upstream regulator analyses were performed using IPA-QIAGEN, allowing a global and interconnected evaluation. …”
Get full text

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE... by Vojtěch Kovařovic, Adéla Finstrlová, Ivo Sedláček, Petr Petráš, Pavel Švec, Ivana Mašlaňová, Meina Neumann-Schaal, Ondrej Šedo, Tibor Botka, Eva Staňková, Jiří Doškař, Roman Pantůček

“…Based on nucleotide and amino acid identity to other known ccr genes and the distinct integration site that differs from the canonical methyltransferase gene rlmH exploited by SCCs, we classified the ccr genes as novel variants, ccrDE. …”
Get full text