3389 Coagulation Factor Xll-mediated contact system and its role in adaptive and innate immune cell movement by Emily Beltran, Tukisa Smith, Beth Graczyk, Jan Breslow, Manish Ponda

“…METHODS/STUDY POPULATION: To test whether the addition of HK-D5 peptide would accelerate C-C chemokine receptor type 2 (CCR2)-mediated chemotaxis in vitro, a real-time transwell chemotaxis assay was developed utilizing human THP-1 monocyte cell line (Fig 1). …”
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Polarization and migration in the zebrafish posterior lateral line system. by Hildur Knutsdottir, Cole Zmurchok, Dhananjay Bhaskar, Eirikur Palsson, Damian Dalle Nogare, Ajay B Chitnis, Leah Edelstein-Keshet

“…We then use a 3D cell-based simulation with realistic cell-cell adhesion, interaction forces, and chemotaxis. Our model is able to reproduce experimentally observed motility with leading cells migrating up a gradient of CXCL12a, and trailing (FGF receptor active) cells moving actively by chemotaxis towards FGF ligand secreted by the leading cells. …”
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Three-dimensional cancer cell migration directed by dual mechanochemical guidance by Pedram Esfahani, Herbert Levine, Mrinmoy Mukherjee, Bo Sun

“…When contact guidance is parallel to the chemical gradient, coherent ECM fibers significantly increase the efficiency of chemotaxis. When contact guidance is perpendicular to the chemical gradient, cells exploit the ECM disorder to locate paths for chemotaxis. …”
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Micro-scale interactions between chemotactic bacteria and algae by Vahora, Nisha

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New methods to study macrophage biology in vitro and in vivo by Rumianek, AN

“…Using the FF chemotaxis assay, I observed novel properties of macrophage behaviour such as population-level coordination of chemotaxis and the ability to sense the gradients both spatially and temporally. …”

Modelling of macrophages interactions in breast cancer by partial differential equations by Admon, Mohd. Rashid

“…Tthe signalling interaction between tumor cells and macrophages will form spontaneous aggregation that causes tumor spreading. tumor cells and macrophages interchange their respective signals which results a paracrine and autocrine signalling loop. this interaction process can be represented by mathematical model in the form of chemotaxis and reaction diffusion equations. the existing models that consider paracrine signalling loop alone or with the inclusion of paracrine and autocrine signalling loop had been developed by assuming linear signals production. however, this assumption does not give a better representation on the signal dynamics where it is supposed to be in nonlinear form that saturate with increasing cell densities. therefore, in this research, two existing interaction models are improved by considering the nonlinear form of signals production. besides, another new interaction model is also developed based on the facts that tumor cells release enzyme during the signaling interaction to penetrate the surrounding tissues. the stability analysis is conducted on three separated models to investigate the condition for spontaneous aggregation. each of these conditions then are validated using numerical simulations. stability analysis shows that for all models, the formation of aggregation could be determined by the parameter that represents the secretion and degradation rates of signals together with chemotaxis rates towards signals. however, the inclusion of autocrine signalling loop in the second model increase the possibility of the aggregation. while in the third model, an additional parameter that represents the secretion and degradation rates of enzyme as well as chemotaxis rates towards them could also determine the formation of the aggregation. by numerical simulations, the results are in agreement with the stability analysis obtained for each of the interaction models. besides, cell clusters that result from the aggregation will be merged to the other cells cluster due to the “effective attraction” between them. reducing the production rates of signal or chemotaxis rates towards signals or increasing degradation rates of signal is required to prevent aggregation. the same changes towards enzymes will give the same result on preventing the aggregation. these valuable suggestions are crucial for medical experts during treatments.…”
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Re-engineering bacterial two-component signalling systems by Blades, G

“…TCS are used to navigate to nutrients or away from toxins (chemotaxis) and to adapt to changes in osmolarity (osomosensing). …”

CRTH2 mediates the activation of human Th2 cells in response to PGD(2) released from IgE/anti-IgE treated nasal polyp tissue. by Pérez-Novo, C, Holtappels, G, Vinall, S, Xue, L, Zhang, N, Bachert, C, Pettipher, R

“…Supernatants from IgE/anti-IgE-stimulated nasal polyp tissue caused significantly increased chemotaxis of Th2 cells. The levels of PGD(2) produced and the degree of Th2 cell chemotaxis were highly correlated. …”

Tracking bifurcating solutions of a model biological pattern generator by Winters, K, Myerscough, M, Maini, P, Murray, J

“…We study heterogeneous steady-state solutions of a cell-chemotaxis model for generating biological spatial patterns in two-dimensional domains with zero flux boundary conditions. …”

Specificity of localization and phosphotransfer in the CheA proteins of Rhodobacter sphaeroides. by Scott, K, Porter, S, Bagg, E, Hamer, R, Hill, J, Wilkinson, D, Armitage, J

“…Three CheA homologues are essential for chemotaxis in R. sphaeroides under laboratory conditions. …”

Tracking bifurcating solutions of a model biological pattern generator by Winters, K, Myerscough, MR, Maini, P, Murray, J

“…We study heterogeneous steady-state solutions of a cell-chemotaxis model for generating biological spatial patterns in two-dimensional domains with zero flux boundary conditions. …”

HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression by Jasmina Makarević, Jochen Rutz, Eva Juengel, Sebastian Maxeiner, Jens Mani, Stefan Vallo, Igor Tsaur, Frederik Roos, Felix K.-H. Chun, Roman A. Blaheta

“…Parental (par) and temsirolimus-resistant (res) PC3 prostate cancer cells were exposed to the HDAC inhibitor valproic acid (VPA), and tumor cell adhesion, chemotaxis, migration, and invasion were evaluated. …”
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Effect of selective phosphodiesterase inhibitors on the rat eosinophil chemotactic response in vitro by Alessandra C Alves, Ana Lucia A Pires, Vincent Lagente, Renato SB Cordeiro, Marco A Martins, Patricia MR e Silva

“…In the present study, we have performed a comparative analysis of the effect of selective inhibitors of phosphodiesterase (PDE) type III, IV and V on eosinophil chemotaxis triggered by platelet activating factor (PAF) and leukotriene B4 (LTB4) in vitro. …”
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Effect of selective phosphodiesterase inhibitors on the rat eosinophil chemotactic response in vitro by Alves Alessandra C, Pires Ana Lucia A, Lagente Vincent, Cordeiro Renato SB, Martins Marco A, Silva Patricia MR e

“…In the present study, we have performed a comparative analysis of the effect of selective inhibitors of phosphodiesterase (PDE) type III, IV and V on eosinophil chemotaxis triggered by platelet activating factor (PAF) and leukotriene B4 (LTB4) in vitro. …”
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Assessment of peripheral neutrophil functions in patients with localized aggressive periodontitis in the Indian population by Rahul S Bhansali, R K Yeltiwar, K G Bhat

“…The aim of the present study was to evaluate neutrophils chemotaxis, phagocytosis, microbicidal activity and superoxide generation in LAP patients of Indian origin. …”
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Submicron 3-D mass spectrometry imaging reveals an asymmetric molecular distribution on chemotaxing cells [version 1; peer review: 2 approved] by Anthony Castellanos, Richard H Gomer, Francisco Fernandez-Lima

“…When starved, D. discoideum cells aggregate into streams of cells in a process called chemotaxis. In this report, we studied D. discoideum cells during chemotaxis using 3D - mass spectrometry imaging (3D-MSI). …”
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Chemokine binding to tenascin-C influences chemokine-induced immune cell migration by Domaingo, A, Jokesch, P, Schweiger, A, Gschwandtner, Midwood, KIM

“…Pre-incubation of the immune cells with TNC resulted in a 3.5-fold increase of CCL26-induced eosinophil chemotaxis, whereas a 1.3-fold de-crease in chemotaxis was observed when monocytes were pre-incubated with CCL2. …”

Bend propagation in the flagella of migrating human sperm by Smith, D, Gaffney, E, Gadelha, H, Kapur, N, Kirkman-Brown, J

“…Assessment of motility in physiological viscosities will be essential in future basic research, studies of chemotaxis and novel diagnostics.…”

Bend propagation in the flagella of migrating human sperm, and its modulation by viscosity. by Smith, D, Gaffney, E, Gadêlha, H, Kapur, N, Kirkman-Brown, J

“…Assessment of motility in physiological viscosities will be essential in future basic research, studies of chemotaxis and novel diagnostics.…”

Physical ecology of marine microbes by Son, Kwangmin

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