Transformation of barley microspores by electroporation by Zlata LUTHAR

“…The pBI121 plasmid contains b-glucuronidase (GUS) reporter gene, NPT II - neomycin phosphotransferase II gene conferring kanamycin resistance, and promoters, NOS (nopaline synthase) and 35S from the cauliflower mosaic virus (CaMV); the pAKC plasmid contains DHDPS (14 kb) - dihydrodipicolinate gene for high lysine content in the seeds, and NPT II resistance gene; and the pAK-CLP plasmid contains a modified DHDPS (7 kb) gene ending protein with leader sequences for chloroplast and AACCl - acetyltransferase gene conferring gentamycin resistance, under the control of the CaMV 35S promoter. …”
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Divergencia entre las subespecies del complejo Alstroemeria garaventae Ehr. Bayer (Alstroemeriaceae) sobre la base de datos cariotípicos y de secuencias de ADN cloroplastidial by Carlos Baeza Perry, Oscar Toro-Núñez, Eduardo Ruiz, David Santos, Ariel Salvatierra, Eitel Thielemann

“…Bayer in Chile was conducted using chromosomes and chloroplast DNA as sources of taxonomic evidence. Both characters indicate both subspecies as separate entities, supporting previous taxonomic hypotheses. …”
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The complete plastid gentome of Dendrocalamus yunnanicus (Poaceae, Bambuseae) by Tian-You He, Ling-Yan Chen, Jing Ye, Biao Yin, Yu-Shan Zheng

“…In this study, the complete chloroplast genome of D. yunnanicus was determined from Illumina HiSeq pair-end sequencing data. …”
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Characterization and phylogenetic analysis of the complete plastome of Maclura tricuspidata (Moraceae) by Xin-Xin Zhu, Rong Wang, Xue-Jie Zhang

“…The complete chloroplast genome (plastome) of Maclura tricuspidate Carriere, a thorny and deciduous tree, is determined. …”
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Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds by Mok Chuen-shing, Mak Chun-yin

“…Two polymerase chain reaction (PCR) systems were developed to amplify a region of the chloroplast DNA and the nuclear phosphoribulokinase (PRK) region specific to <it>Lodoicea maldivica </it>respectively. …”
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Characterization and phylogenetic analysis of the complete plastome of Veronica undulata (Plantaginaceae) by Kun Liu, Shou-Jin Fan

“…Veronica undulata is a perennial herb, and the complete chloroplast genome (plastome) of V. undulata was determined in this study. …”
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Characterization and phylogenetic analysis of the complete plastome of Amaranthus retroflexus L. (Amaranthaceae), an annual weeds by Ben-Xia Lou, Shou-Jin Fan

“…A total of 113 genes were identified, including 79 protein-coding genes, four rRNA genes, and 30 tRNA genes. Twenty chloroplast genomes from Amaranthaceae were selected to reconstruct phylogenetic tree and the result supported that A. retroflexus was sister to A. hypochondriacus and A. caudatus.…”
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The complete mitochondrial genome of Dumortiera hirsuta (Sw.) Nees (Dumortieraceae, Marchantiophyta) by Woochan Kwon, Yongsung Kim, Jongsun Park

“…Phylogenetic trees show that topology agrees with that of phylogenetic tree of chloroplast genome as well as taxonomical classification.…”
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<em>Dasylirion</em> systematics: taxonomy and molecular phylogeny by David J. Bogler

“…Sixteen species, including four new ones, were recognized. Data from chloroplast DNA restriction site analysis suggest that the species of Dasylirion in southern Mexico are basal in the genus, that Nolina parviflora is particularly close to both Dasylirion and Beaucarnea, and that Calibanus is closest to Beaucarnea. …”
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Artemisia qingheensis (Asteraceae, Anthemideae), a new species from Xinjiang, China by Guang-Zhao Jin, Mariya Sheludyakova, Wen-Jun Li, Feng Song, Zhi-Bin Wen, Ying Feng

“…We investigated its phylogenetic position and relationships with 35 other species of Artemisia using whole chloroplast DNA sequence data. The molecular phylogenetic results and morphological evidence (multi-layered involucral bracts and homogamous capitula with bisexual flowers) showed that the new species belongs to Artemisia subgenus Seriphidium. …”
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Hemiboea kaiyangensis, a new species of Gesneriaceae endemic to Guizhou, China by Tao Peng, Shun-Zhi He, Shun-Li Wang, Dan Huang, Xu-Ping Zhou

“…We investigated its phylogenetic position and relationships with 13 other species of Hemiboea C.B.Clarke, which present large morphological diversity in the genus, based on molecular analyses of the nuclear ribosomal internal transcribed spacer (ITS) and the chloroplast trnL-F intron-spacer sequences. The molecular phylogenetic analyses revealed that the new species is most closely related to H. ovalifolia. …”
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Sweet potato and wild relatives - Molecular data by Rodriguez, P, Scotland, R

“…The dataset consists of three types of DNA sequences generated through high-throughput sequencing: whole chloroplast genomes, single-copy nuclear coding regions, and the non-coding ribosomal Internal Transcribed Spacer (ITS). …”

Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis by Siritanaratkul, B, Megarity, C, Roberts, T, Samuels, T, Winkler, M, Warner, J, Happe, T, Armstrong, F

“…In a discovery of the transfer of chloroplast biosynthesis activity to an inorganic material, ferredoxin-NADP+ reductase (FNR), the pivotal redox flavoenzyme of photosynthetic CO2 assimilation, binds tightly within the pores of indium tin oxide (ITO) to produce an electrode for direct studies of the redox chemistry of the FAD active site, and fast, reversible and diffusion-controlled interconversion of NADP+ and NADPH in solution. …”

Disentangling <i>Crocus</i> Series <i>Verni</i> and Its Polyploids by Irena Raca, Frank R. Blattner, Nomar Espinosa Waminal, Helmut Kerndorff, Vladimir Ranđelović, Dörte Harpke

“…The multi-data approach we used here for series <i>Verni</i>, combining evidence from nuclear and chloroplast phylogenies, genome sizes, chromosome numbers, and genomic heterozygosity for ploidy estimations, provides a way to disentangle the evolution of plant taxa with complex karyotype changes that can be used for the analysis of other groups within <i>Crocus</i> and beyond. …”
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The Single-Stranded DNA-Binding Gene <i>Whirly</i> (<i>Why1</i>) with a Strong Pathogen-Induced Promoter from <i>Vitis pseudoreticulata</i> Enhances Resistance to <i>Phytophthora c... by Chengchun Lai, Qiuxia Que, Ruo Pan, Qi Wang, Huiying Gao, Xuefang Guan, Jianmei Che, Gongti Lai

“…Both <i>Why1</i> gene sequences had seven exons and six introns, and they had a C-terminal Whirly conserved domain and N-terminal chloroplast transit peptide, which was then verified to be chloroplast localization. …”
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Complete mitochondrial genome of the endangered Prunus pedunculata (Prunoideae, Rosaceae) in China: characterization and phylogenetic analysis by Qian Liu, Zinian Wu, Zinian Wu, Chunyu Tian, Yanting Yang, Lemeng Liu, Yumei Feng, Zhiyong Li, Zhiyong Li

“…A total of 262 potential RNA editing sites in 26 PCGs were identified. Furthermore, 56 chloroplast-derived fragments were ascertained in the mitogenome, ranging from 30 to 858 bp, and were mainly located across IGS (intergenic spacer) regions or rRNA genes. …”
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Cross species/genera transferability of simple sequence repeat markers, genetic diversity and population structure analysis in gladiolus (Gladiolus × grandiflorus L.) genotypes by Varun Hiremath, Kanwar Pal Singh, Neelu Jain, Kishan Swaroop, Pradeep Kumar Jain, Sapna Panwar, Nivedita Sinha

“…Methods In the present investigation, we screened 26 genomic SSRs (Gladiolus palustris, Crocus sativus, Herbertia zebrina, Sysirinchium micranthum), 14 chloroplast SSRs (Gladiolus spp., chloroplast DNA regions) and 25 Iris Expressed Sequence Tags (ESTs) derived SSRs across the 84 gladiolus (Gladiolus × grandiflorus L.) genotypes. …”
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WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications by Minh Bui Manh, Charlotte Ost, Edgar Peiter, Bettina Hause, Karin Krupinska, Klaus Humbeck

“…WHIRLY1, a small plant-specific ssDNA-binding protein, dually located in chloroplasts and the nucleus, is discussed to act as a retrograde signal transmitting a stress signal from the chloroplast to the nucleus and triggering there a stress-related gene expression. …”
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Identification, Expression Analysis and Cloning of Capsicum chinense BCAT Gene Family by Bingchun XIE, Junlin HUANG, Songsen WEN, Tao LI, Ying LI, Xiaowan XU, Xiaomei XU, Zhiming1 WU, Zhou HENG

“…Subcellular localization prediction results showed that CcBCAT1 was located in chloroplast and mitochondria, and other gene family members were located in chloroplast. …”
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Functional analysis of CqPORB in the regulation of chlorophyll biosynthesis in Chenopodium quinoa by Chao Li, Minyuan Ran, Minyuan Ran, Jianwei Liu, Xiaoxiao Wang, Xiaoxiao Wang, Qingbing Wu, Qingbing Wu, Qiang Zhang, Jing Yang, Feng Yi, Heng Zhang, Jian-Kang Zhu, Chunzhao Zhao

“…Subcellular localization analysis indicates that CqPORB is exclusively localized in chloroplast. Together, our study elucidates the important role of CqPORB in the regulation of Chl biosynthesis and chloroplast development in quinoa. …”
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