Combinatorial nanococktails via self-assembling lipid prodrugs for synergistically overcoming drug resistance and effective cancer therapy by Tongyu Li, Weiwei Shi, Jie Yao, Jingyun Hu, Qiong Sun, Jing Meng, Jian Wan, Haihan Song, Hangxiang Wang

“…Results The coassembled nanoparticle cocktails were feasibly fabricated and further refined with an amphiphilic matrix to form a systemically injectable and PEGylated nanomedicine with minimal excipients. …”
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Effect of epitope variant co-delivery on the depth of CD8 T cell responses induced by HIV-1 conserved mosaic vaccines by Edmund G. Wee, Nathifa Moyo, Zara Hannoun, Elena E. Giorgi, Bette Korber, Tomáš Hanke

“…Numerous strategies have been proposed, of which the most promising include focusing responses on the vulnerable sites of HIV-1 displaying the least entropy among global isolates and using algorithms that maximize vaccine match to circulating HIV-1 variants by vaccine cocktails of optimized complementing sequences. In this study, we investigated CD8 T cell responses induced by a bi-valent mosaic of highly conserved HIVconsvX regions delivered by a combination of simian adenovirus ChAdOx1 and poxvirus MVA. …”
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Anti-CfaE nanobodies provide broad cross-protection against major pathogenic enterotoxigenic Escherichia coli strains, with implications for vaccine design by Alla Amcheslavsky, Aaron L. Wallace, Monir Ejemel, Qi Li, Conor T. McMahon, Matteo Stoppato, Serena Giuntini, Zachary A. Schiller, Jessica R. Pondish, Jacqueline R. Toomey, Ryan M. Schneider, Jordan Meisinger, Raimond Heukers, Andrew C. Kruse, Eileen M. Barry, Brian G. Pierce, Mark S. Klempner, Lisa A. Cavacini, Yang Wang

“…Nanobodies are successful new biologics in treating mucosal infectious disease as they recognize conserved epitopes on hypervariable pathogens. Cocktails consisting of multiple nanobodies could provide even broader epitope coverage at a lower cost compared to monoclonal antibodies. …”
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Characterizing the Inflammatory Profile of Neutrophil-Rich Triple-Negative Breast Cancer by Fatma Al Qutami, Walaa AlHalabi, Aswathy Vijayakumar, Surendra Singh Rawat, Abubakr H. Mossa, Manju Nidagodu Jayakumar, Baila Samreen, Mahmood Y. Hachim

“…Flow cytometry showed comparable expression of inflammatory markers by polymorphonuclear cells (PMN) when treated with MDA-MB-231-CM and standard polarizing cocktails. Furthermore, MDA-MB-231-CM triggered an inflammatory pattern with evidence of stronger adhesion (CD62L) and degranulation (CD11b and CD66b) phenotypes. …”
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Myceliophthora thermophila Xyr1 is predominantly involved in xylan degradation and xylose catabolism by Ana Carolina dos Santos Gomes, Daniel Falkoski, Evy Battaglia, Mao Peng, Maira Nicolau de Almeida, Nancy Coconi Linares, Jean-Paul Meijnen, Jaap Visser, Ronald P. de Vries

“…Abstract Background Myceliophthora thermophila is a thermophilic ascomycete fungus that is used as a producer of enzyme cocktails used in plant biomass saccharification. Further development of this species as an industrial enzyme factory requires a detailed understanding of its regulatory systems driving the production of plant biomass-degrading enzymes. …”
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Shutting off the fuel supply to target metabolic vulnerabilities in multiple myeloma by Priyanka S. Rana, Priyanka S. Rana, Krishna Goparaju, Krishna Goparaju, James J. Driscoll, James J. Driscoll, James J. Driscoll

“…A better understanding of the events that reprogram metabolism in myeloma and immune cells may reveal unforeseen vulnerabilities and advance the rational design of drug cocktails that improve patient survival.…”
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Decellularized squid mantle scaffolds as tissue‐engineered corneal stroma for promoting corneal regeneration by Honghua Kang, Yi Han, Mengyi Jin, Lan Zheng, Zhen Liu, Yuhua Xue, Zuguo Liu, Cheng Li

“…Subsequently, a novel clearing method, namely clear, unobstructed brain imaging cocktails (CUBIC) method was used to transparent it. …”
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Fate and Growth Kinetics of Salmonella and Listeria monocytogenes on Mangoes During Storage by Joyjit Saha, Zeynal Topalcengiz, Vijendra Sharma, Loretta M. Friedrich, Michelle D. Danyluk

“…Whole mangoes were spot inoculated with rifampicin-resistant pathogen cocktails (6 log CFU/mango) onto the midsection of whole fruit (n = 6). …”
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Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from <i>Plasmodium</i> Challenge in a Mouse Model by Sophia M. Reeder, Emma L. Reuschel, Mamadou A. Bah, Kun Yun, Nicholas J. Tursi, Kevin Y. Kim, Jacqueline Chu, Faraz I. Zaidi, Ilknur Yilmaz, Robert J. Hart, Benjamin Perrin, Ziyang Xu, Laurent Humeau, David B. Weiner, Ahmed S. I. Aly

“…To further evaluate protection mediated by LS antigens, we focused on exported pre-erythrocytic proteins (exported protein 1 (EXP1), profilin (PFN), exported protein 2 (EXP2), inhibitor of cysteine proteases (ICP), transmembrane protein 21 (TMP21), and upregulated in infective sporozoites-3 (UIS3)) expressed in all <i>Plasmodium</i> species and designed optimized, synthetic DNA (synDNA) immunogens. SynDNA antigen cocktails were tested with and without the molecular adjuvant plasmid IL-33. …”
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Extracellular Vesicles from <i>Bothrops jararaca</i> Venom Are Diverse in Structure and Protein Composition and Interact with Mammalian Cells by Larissa Gonçalves-Machado, Brunno Renato Farias Verçoza, Fábio César Sousa Nogueira, Rafael Donadélli Melani, Gilberto Barbosa Domont, Silas Pessini Rodrigues, Juliany Cola Fernandes Rodrigues, Russolina Benedeta Zingali

“…Snake venoms are complex cocktails of non-toxic and toxic molecules that work synergistically for the envenoming outcome. …”
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Antibiotics Modulate Intestinal Regeneration by Lymarie M. Díaz-Díaz, Natalia Rosario-Meléndez, Andrea Rodríguez-Villafañe, Yariel Y. Figueroa-Vega, Omar A. Pérez-Villafañe, Angela M. Colón-Cruz, Paola I. Rodríguez-Sánchez, Julio M. Cuevas-Cruz, Sonya J. Malavez-Cajigas, Sergio M. Maldonado-Chaar, José E. García-Arrarás

“…For this, holothurians were eviscerated and left to regenerate for 10 days in seawater with different penicillin/streptomycin-based cocktails (100 µg/mL PS) including: 100 µg/mL kanamycin (KPS), 5 µg/mL vancomycin (VPS), and 4 µg/mL (E4PS) or 20 µg/mL (E20PS) erythromycin. …”
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Brief Local Application of Progesterone via a Wearable Bioreactor Induces Long-Term Regenerative Response in Adult Xenopus Hindlimb by Celia Herrera-Rincon, Annie S. Golding, Kristine M. Moran, Christina Harrison, Christopher J. Martyniuk, Justin A. Guay, Julia Zaltsman, Hayley Carabello, David L. Kaplan, Michael Levin

“…Our experiments establish a model for testing therapeutic cocktails in vertebrate hindlimb regeneration, identify pro-regenerative activities of progesterone-containing bioreactors, and provide proof of principle of brief use of integrated device-based delivery of small-molecule drugs as a viable strategy to induce and maintain a long-term regenerative response. : The complexity of vertebrate limbs drives the search for regenerative treatments that trigger endogenous processes of repair. …”
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Direct Neuronal Reprogramming: Bridging the Gap Between Basic Science and Clinical Application by Lakshmy Vasan, Lakshmy Vasan, Eunjee Park, Eunjee Park, Luke Ajay David, Luke Ajay David, Taylor Fleming, Carol Schuurmans, Carol Schuurmans, Carol Schuurmans

“…The striking observation that postmitotic, terminally differentiated somatic cells can be converted to iNs by mis-expression of TFs or miRNAs involved in neural lineage development, and/or by exposure to growth factors or small molecule cocktails that recapitulate the signaling environment of the developing brain, has opened the door to the rapid expansion of new neuronal reprogramming methodologies. …”
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Distribution of CRISPR Types in Fluoroquinolone-Resistant <i>Campylobacter jejuni</i> Isolates by Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin

“…These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant <i>Campylobacter</i>.…”
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Molecular mechanisms and design principles for promiscuous inhibitors to avoid drug resistance: Lessons learned from HIV-1 protease inhibition by Shen, Yang, Radhakrishnan, Mala L., Tidor, Bruce

“…As no inhibitor bound to all variants, we designed small cocktails of inhibitors to do so and discovered that they often jointly covered the target set through mechanistic complementarity. …”
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Using an Inducible Promoter of a Gene Encoding Penicillium verruculosum Glucoamylase for Production of Enzyme Preparations with Enhanced Cellulase Performance. by Alexander G Bulakhov, Pavel V Volkov, Aleksandra M Rozhkova, Alexander V Gusakov, Vitaly A Nemashkalov, Aidar D Satrutdinov, Arkady P Sinitsyn

“…Content of the heterologous AnBGL in the secreted multienzyme cocktails (hBGL1, hBGL2 and hBGL3) varied from 4 to 10% of the total protein, while the content of TrLPMO in the hLPMO sample was ~3%. …”
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Investigation of prevalence of free Shiga toxin-producing Escherichia coli (STEC)-specific bacteriophages and its correlation with STEC bacterial hosts in a produce-growing area in... by Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu

“…Surface water from 20 samples sites was subjected to free bacteriophage isolation using host strains of both generic E. coli and STEC (O157, six non-O157 and one O179 strains) cocktails, and isolation of O157 and non-O157 STEC strains by use of culture methods combined with PCR-based confirmation. …”
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Transcriptional Response of <i>Salmonella enterica</i> to Bacteriophage Treatments with Differential Multiplicities of Infection by Catherine W. Y. Wong, Siyun Wang

“…Therefore, bacteriophages have been suggested as an antimicrobial treatment against <i>S. enterica</i>, but it is currently unclear if there is an optimal bacteriophage multiplicity of infection (MOI) against <i>S. enterica</i>. Two bacteriophage cocktails at MOIs 1, 10, 100, 1000 and 10,000 were co-inoculated against four <i>S. enterica</i> strains (<i>S.…”
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Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice by Jiaoyu Shi, Yuan Hu, Guangze Shao, Yixiang Zhu, Zhonghua Zhao, Yanyong Xu, Zhigang Zhang, Huijuan Wu

“…In this study, clear, unobstructed brain imaging cocktails and computational analysis (CUBIC), one of three-dimensional optical clearing approaches, was used which combines tissue clearing, immunolabeling, and a light-sheet microscope to image and evaluate podocytes in C57BL/6 (C57) and db/db mice. …”
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The nature and combination of subunits used in epitope-based <it>Schistosoma japonicum </it>vaccine formulations affect their efficacy by Liu Feng, He Lei, Wen Xiaoyun, Zhou Sha, Hoellwarth Jason, Chi Ying, Zhang Lei, Wang Xuefeng, Wu Calvin, Su Chuan

“…</p> <p>Results</p> <p>In this study, we developed PDDV cocktails containing multiple epitopes of <it>S. japonicum </it>from Sj22.6, Sj62 and Sj97 antigens by predicting cytotoxic, helper, and B-cell epitopes, and evaluated vaccine potential <it>in vivo</it>. …”
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