Protein Engineering of Endoglucanase CelR of Clostridium thermocellum for Enhanced Expression by Hafiz Muzzammel Rehman, Hira Nasir, Adnan Iqbal, Syed Zawar Shah, Muhammad Umair Naseem

“…The cellulase genes from the confirmed transformed plasmids were sub-cloned in T7 promoter-based expression vector pET-28a and expression analysis was done in E. coli (DE3) BL21 codon Plus. Results: An SDS PAGE analysis of both the CelR derivatives revealed that the truncated version i.e. …”
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Fluoroquinolone resistance mutations among Mycobacterium tuberculosis and their interconnection with treatment outcome by Ramakant Dixit, Emil Mohan, Ankur Gupta, Priyanka Soni Gupta, Tarun Patni, Mukesh Goyal, Roshan Kumar Meena

“…Conclusions: GyrA MUT3C hybridization corresponding to single-point mutation of aspartic acid to glycine at codon 94 constitutes the most common mutation in GyrA gene locus in M. tuberculosis with significant association with treatment outcome as died compared to those with treatment outcome as cured.…”
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Simultaneous Bilateral Testicular Metastases from Renal Clear Cell Carcinoma: A Rare Presentation in Von Hippel–Lindau disease by Asaad Moradi, Delaram Farhoumand, Behnaz Bouzari, Behnam Shakiba

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Genetic Characterization, Agro-Morphological and Physiological Evaluation of Grafted Tomato under Salinity Stress Conditions by Emad A. Abdeldym, Mohamed M. El-Mogy, Hoda R. L. Abdellateaf, Mohamed A. M. Atia

“…At the molecular level, two robust gene-targeting marker systems (Conserved DNA-Derived Polymorphism; CDDP and Start Codon Targeted Polymorphism; SCoT) were employed. …”
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Construction and periplasmic expression of a bispecific tandem scFv for dual targeting of immune checkpoints by Amirreza Rashti, Vajihe Akbari

“…Materials and Methods: The bispecific tandem scFv was constructed based on the variable regions gene of anti-PD1 and anti-CTLA-4 antibodies. The optimum codon for expression in E. coli was chemically synthesized and subcloned in periplasmic expression plasmid. …”
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Inoperable Renal Malignant Glomus Tumor, the answers for all the “W’s”? by Pushkala Surianarayanan, Arun Ramdas Menon, Shriley Sundersingh, Anand Raja

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Lack of EGFR mutations benefiting gefitinib treatment in adenocarcinoma of esophagogastric junction by Wang Wen-Ping, Wang Kang-Ning, Gao Qiang, Chen Long-Qi

“…</p> <p>Results</p> <p>In exon 20, a variant from CAG to CAA at codon 787 (2361G-> A) was identified in 19 patients, which was a genomic variation of EGFR since it was found in both cancer tissue and white blood cells. …”
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Identifying Aspects of the Post-Transcriptional Program Governing the Proteome of the Green Alga Micromonas pusilla. by Peter H Waltman, Jian Guo, Emily Nahas Reistetter, Samuel Purvine, Charles K Ansong, Marijke J van Baren, Chee-Hong Wong, Chia-Lin Wei, Richard D Smith, Stephen J Callister, Joshua M Stuart, Alexandra Z Worden

“…Several features in the genome sequence were found to influence protein abundance including codon usage as well as 3' UTR length and structure. …”
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Understanding the Mechanism of Diabetes Mellitus in a LRBA-Deficient Patient by Iman Hawari, Johan Ericsson, Basirudeen Syed Ahamed Kabeer, Damien Chaussabel, Asma Alsulaiti, Sanaa A. Sharari, Cristina Maccalli, Faiyaz Ahmad Khan, Khalid Hussain

“…The scope of this study is to show that DM in a LRBA-deficient patient with a stop codon mutation (c.3999 G > A) was not mediated through autoimmunity. …”
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State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, an... by Milica Crnoglavac Popović, Marija Stanišić, Radivoje Prodanović

“…This is always the case in studies dealing with the heterologous expression of oxidoreductase enzymes, since there is a requirement not only for multiple OXR gene integrations into the yeast genome (super transformations), and codon optimization, but also very careful design of fermentation media composition and fermentation conditions during expression due to the need for transition metals (copper and iron) and metabolic precursors of FAD and heme. …”
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Markov Chain-Like Quantum Biological Modeling of Mutations, Aging, and Evolution by Ivan B. Djordjevic

“…In our recent paper published in Life, we have derived the operator-sum representation of a biological channel based on codon basekets, and determined the quantum channel model suitable for study of the quantum biological channel capacity. …”
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The First Genome-Wide Mildew Locus O Genes Characterization in the <i>Lamiaceae</i> Plant Family by Andolfo Giuseppe, Ercolano Maria Raffaella

“…Finally, we performed a codon-based evolutionary analysis, revealing a general high level of purifying selection in the eleven <i>Lamiaceae</i> MLO gene families, and the occurrence of few regions under diversifying selection in candidate susceptibility factors. …”
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Novel homozygous variant in the PDZD7 gene in a family with nonsyndromic sensorineural hearing loss by Qiang Du, Qin Sun, Xiaodong Gu, Jinchao Wang, Weitao Li, Luo Guo, Huawei Li

“…This variant lies in exon 15 of PDZD7 and results in a frame shift followed by an early stop codon. It is classified as pathogenic according to the ACMG/AMP guidelines and ClinGen specifications. …”
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Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora by Lucas S. Hollstein, Kerstin Schmitt, Oliver Valerius, Gertrud Stahlhut, Stefanie Pöggeler

“…For the establishment of BioID in S. macrospora, a codon-optimized TurboID biotin ligase was fused to SCI1. …”
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The Mitochondrial Genome of <i>Littoraria melanostoma</i> Reveals a Phylogenetic Relationship within Littorinimorpha by Kun Chen, Mingliu Yang, Haisheng Duan, Xin Liao

“…The amino acid composition and codon usage of the mitochondrial genomes from seven superfamilies of Littorinimorpha were analyzed, and the results showed that CUU (Leu), GCU (Ala), AUU (Ile), UCU (Ser), UUA (Leu), GUU (Gly), and UUU (Phe) are the commonly used codons. …”
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Mutation of the ALDH7A1 gene in a patient with pyridoxal phosphate-dependent neonatal epileptic encephalopathy: a clinical case by T. V. Kozhanova, S. S. Zhilina, T. I. Mescheryakova, E. G. Lukyanova, K. V. Osipova, S. O. Ayvazyan, A. G. Prityko

“…This mutation led to the appearance of a stop codon in position 82 of the protein p.Arg82Ter and the amino acid substitution in position 399 of the protein p.Glu399Gln. …”
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Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus by Tatsuo Yamaguchi, Tadashi Watanabe, Yuki Iwaisako, Masahiro Fujimuro

“…Here, we investigated the role of ORF21 in lytic replication and infection by generating two ORF21-mutated KSHV BAC clones: ORF21-kinase activity deficient KSHV (21KD) and stop codon-induced ORF21-deleted KSHV (21del). The results showed that both ORF21 mutations did not affect viral genome replication, lytic gene transcription, or the production of viral genome-encapsidated particles. …”
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DeepRaccess: high-speed RNA accessibility prediction using deep learning by Kaisei Hara, Kaisei Hara, Natsuki Iwano, Tsukasa Fukunaga, Michiaki Hamada, Michiaki Hamada, Michiaki Hamada

“…In addition, we confirmed that DeepRaccess could predict protein abundance in E.coli with moderate accuracy from the sequences around the start codon. We also demonstrated that DeepRaccess achieved tens to hundreds of times software speed-up in a GPU environment. …”
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Next-generation sequencing reveals a case of Norrie disease in a child with bilateral ocular malformation by Haijun Li, Zhiming Li, Degang Wang, Chuanming Chen, Zhiqiang Chen, Jinhua Wang, Chenxia Xu, Xingsheng Dong

“…It was concurrently confirmed that the NDP gene variant led to the deletion of 246 bp at the 3′ end of exon 2, resulting in the deletion of the initiation codon and the occurrence of disease. Our study suggests that the diagnosis of rare diseases through next-generation sequencing, combined with prenatal ultrasound and prenatal diagnosis, can help families with known familial genetic diseases. …”
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Genotypic detection of rifampicin-resistant M. tuberculosis strains in Syrian and Lebanese patients by Abdulkader Rahmo, Zahraa Hamdar, Imad Kasaa, Fouad Dabboussi, Monzer Hamze

“…The changes were mostly within codons 531 (37/97: 38%), 533 (28/97: 29%) and 526 (9/97: 9%). …”
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