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2601
Protein Engineering of Endoglucanase CelR of Clostridium thermocellum for Enhanced Expression
Published 2019-02-01“…The cellulase genes from the confirmed transformed plasmids were sub-cloned in T7 promoter-based expression vector pET-28a and expression analysis was done in E. coli (DE3) BL21 codon Plus. Results: An SDS PAGE analysis of both the CelR derivatives revealed that the truncated version i.e. …”
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2602
Fluoroquinolone resistance mutations among Mycobacterium tuberculosis and their interconnection with treatment outcome
Published 2023-01-01“…Conclusions: GyrA MUT3C hybridization corresponding to single-point mutation of aspartic acid to glycine at codon 94 constitutes the most common mutation in GyrA gene locus in M. tuberculosis with significant association with treatment outcome as died compared to those with treatment outcome as cured.…”
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2603
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2604
Genetic Characterization, Agro-Morphological and Physiological Evaluation of Grafted Tomato under Salinity Stress Conditions
Published 2020-12-01“…At the molecular level, two robust gene-targeting marker systems (Conserved DNA-Derived Polymorphism; CDDP and Start Codon Targeted Polymorphism; SCoT) were employed. …”
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2605
Construction and periplasmic expression of a bispecific tandem scFv for dual targeting of immune checkpoints
Published 2023-01-01“…Materials and Methods: The bispecific tandem scFv was constructed based on the variable regions gene of anti-PD1 and anti-CTLA-4 antibodies. The optimum codon for expression in E. coli was chemically synthesized and subcloned in periplasmic expression plasmid. …”
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2606
Inoperable Renal Malignant Glomus Tumor, the answers for all the “W’s”?
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2607
Lack of EGFR mutations benefiting gefitinib treatment in adenocarcinoma of esophagogastric junction
Published 2012-01-01“…</p> <p>Results</p> <p>In exon 20, a variant from CAG to CAA at codon 787 (2361G-> A) was identified in 19 patients, which was a genomic variation of EGFR since it was found in both cancer tissue and white blood cells. …”
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2608
Identifying Aspects of the Post-Transcriptional Program Governing the Proteome of the Green Alga Micromonas pusilla.
Published 2016-01-01“…Several features in the genome sequence were found to influence protein abundance including codon usage as well as 3' UTR length and structure. …”
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2609
Understanding the Mechanism of Diabetes Mellitus in a LRBA-Deficient Patient
Published 2022-04-01“…The scope of this study is to show that DM in a LRBA-deficient patient with a stop codon mutation (c.3999 G > A) was not mediated through autoimmunity. …”
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2610
State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, an...
Published 2024-02-01“…This is always the case in studies dealing with the heterologous expression of oxidoreductase enzymes, since there is a requirement not only for multiple OXR gene integrations into the yeast genome (super transformations), and codon optimization, but also very careful design of fermentation media composition and fermentation conditions during expression due to the need for transition metals (copper and iron) and metabolic precursors of FAD and heme. …”
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2611
Markov Chain-Like Quantum Biological Modeling of Mutations, Aging, and Evolution
Published 2015-08-01“…In our recent paper published in Life, we have derived the operator-sum representation of a biological channel based on codon basekets, and determined the quantum channel model suitable for study of the quantum biological channel capacity. …”
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2612
The First Genome-Wide Mildew Locus O Genes Characterization in the <i>Lamiaceae</i> Plant Family
Published 2023-09-01“…Finally, we performed a codon-based evolutionary analysis, revealing a general high level of purifying selection in the eleven <i>Lamiaceae</i> MLO gene families, and the occurrence of few regions under diversifying selection in candidate susceptibility factors. …”
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2613
Novel homozygous variant in the PDZD7 gene in a family with nonsyndromic sensorineural hearing loss
Published 2022-06-01“…This variant lies in exon 15 of PDZD7 and results in a frame shift followed by an early stop codon. It is classified as pathogenic according to the ACMG/AMP guidelines and ClinGen specifications. …”
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2614
Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora
Published 2022-10-01“…For the establishment of BioID in S. macrospora, a codon-optimized TurboID biotin ligase was fused to SCI1. …”
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2615
The Mitochondrial Genome of <i>Littoraria melanostoma</i> Reveals a Phylogenetic Relationship within Littorinimorpha
Published 2023-09-01“…The amino acid composition and codon usage of the mitochondrial genomes from seven superfamilies of Littorinimorpha were analyzed, and the results showed that CUU (Leu), GCU (Ala), AUU (Ile), UCU (Ser), UUA (Leu), GUU (Gly), and UUU (Phe) are the commonly used codons. …”
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2616
Mutation of the ALDH7A1 gene in a patient with pyridoxal phosphate-dependent neonatal epileptic encephalopathy: a clinical case
Published 2019-04-01“…This mutation led to the appearance of a stop codon in position 82 of the protein p.Arg82Ter and the amino acid substitution in position 399 of the protein p.Glu399Gln. …”
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2617
Kaposi’s Sarcoma-Associated Herpesvirus ORF21 Enhances the Phosphorylation of MEK and the Infectivity of Progeny Virus
Published 2023-01-01“…Here, we investigated the role of ORF21 in lytic replication and infection by generating two ORF21-mutated KSHV BAC clones: ORF21-kinase activity deficient KSHV (21KD) and stop codon-induced ORF21-deleted KSHV (21del). The results showed that both ORF21 mutations did not affect viral genome replication, lytic gene transcription, or the production of viral genome-encapsidated particles. …”
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2618
DeepRaccess: high-speed RNA accessibility prediction using deep learning
Published 2023-10-01“…In addition, we confirmed that DeepRaccess could predict protein abundance in E.coli with moderate accuracy from the sequences around the start codon. We also demonstrated that DeepRaccess achieved tens to hundreds of times software speed-up in a GPU environment. …”
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2619
Next-generation sequencing reveals a case of Norrie disease in a child with bilateral ocular malformation
Published 2022-08-01“…It was concurrently confirmed that the NDP gene variant led to the deletion of 246 bp at the 3′ end of exon 2, resulting in the deletion of the initiation codon and the occurrence of disease. Our study suggests that the diagnosis of rare diseases through next-generation sequencing, combined with prenatal ultrasound and prenatal diagnosis, can help families with known familial genetic diseases. …”
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2620
Genotypic detection of rifampicin-resistant M. tuberculosis strains in Syrian and Lebanese patients
Published 2012-12-01“…The changes were mostly within codons 531 (37/97: 38%), 533 (28/97: 29%) and 526 (9/97: 9%). …”
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