Genome-wide polysomal analysis of a yeast strain with mutated ribosomal protein S9 by Arava Yoav, Pnueli Lilach

“…Read-through analysis did not reveal any increase in read-through of a premature stop codon by the mutant strain.</p> <p>Conclusion</p> <p>The ribosomal protein rpS9 appears to be involved in the translation of many mRNAs, since altering its C terminus led to a significant change in ribosomal association of many mRNAs. …”
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Kaposi’s Sarcoma-Associated Herpesvirus ORF7 Is Essential for Virus Production by Yuki Iwaisako, Tadashi Watanabe, Mizuki Hanajiri, Yuichi Sekine, Masahiro Fujimuro

“…Here, we investigated the role of ORF7 during KSHV lytic replication by generating two types of ORF7 knock-out (KO) mutants (frameshift-induced and stop codon-induced ORF7 deficiency), KSHV BAC16, and its revertants. …”
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Role of ribosome recycling factor in natural termination and translational coupling as a ribosome releasing factor. by Yoshio Inokuchi, Fabio Quaglia, Akikazu Hirashima, Yoshihiro Yamamoto, Hideko Kaji, Akira Kaji

“…These in vivo and in vitro studies indicate that RRF releases ribosomes from mRNA at the termination codon of u-ORF. Furthermore, the non-dissociable ribosomes read downstream from AUG of UAAUG with RRF in vitro. …”
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Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer’s and Creutzfeldt–Jakob Disease: A Micromorphological Pilot Study on 20 Brains by Nikol Jankovska, Tomas Olejar, Radoslav Matej

“…The AD ABC score according to the NIA-Alzheimer’s association guidelines, and prion protein subtype with codon 129 methionine–valine (M/V) polymorphisms in sCJD, while representing key characteristics of these diseases, did not correlate with the morphology of the Aβ/PrP^Sc co-aggregates. …”
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Molecular Detection of Dapsone and Rifampicin Resistance on Mycobacterium leprae from Leprosy Patients in East Java by DINAR ADRIATY, RATNA WAHYUNI, CITA ROSITA S. PRAKOESWA, NI PUTU SUSARI, INDROPO AGUSNI, SHINZO IZUMI

“…From 70 isolates for folP gene examination, there were 3 isolates which had mutation in the amino acid at codon 53; 2 cases Threonin (ACC) became Alanin (GCC) and 1 case Threonin (ACC) became Arginin (AGA). …”
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Virulence Structure and Genetic Diversity of <i>Blumeria graminis</i> f. sp. <i>avenae</i> from Different Regions of Europe by Magdalena Cieplak, Aleksandra Nucia, Tomasz Ociepa, Sylwia Okoń

“…<i>avenae</i> was assessed based on 80 single-spore isolates collected in different European countries such as Poland (40 isolates), Germany (10), Finland (10), Czech Republic (10) and Ireland (10) using ISSR (<i>Inter-Simple Sequence Repeats</i>) and SCoT (<i>Start Codon Targeted</i>) markers. This work demonstrated differences in virulence of <i>B. graminis</i> f. sp. …”
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Identification of HDV-like theta ribozymes involved in tRNA-based recoding of gut bacteriophages by Kasimir Kienbeck, Lukas Malfertheiner, Susann Zelger-Paulus, Silke Johannsen, Christian von Mering, Roland K. O. Sigel

“…Bacteriophages, the dominant virome constituents, can utilize suppressor tRNAs to switch to alternative genetic codes (e.g., the UAG stop-codon is reassigned to glutamine) while infecting hosts with the standard bacterial code. …”
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A LINE-1 insertion in DLX6 is responsible for cleft palate and mandibular abnormalities in a canine model of Pierre Robin sequence. by Zena T Wolf, Elizabeth J Leslie, Boaz Arzi, Kartika Jayashankar, Nili Karmi, Zhonglin Jia, Douglas J Rowland, Amy Young, Noa Safra, Saundra Sliskovic, Jeffrey C Murray, Claire M Wade, Danika L Bannasch

“…The LINE-1 insertion is predicted to insert a premature stop codon within the homeodomain of DLX6. This prompted the sequencing of DLX5 and DLX6 in a human cohort with CP, where a missense mutation within the highly conserved DLX5 homeobox of a patient with PRS was identified. …”
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An insight into the sialotranscriptome of the West Nile mosquito vector, <it>Culex tarsalis</it> by Olson Kenneth E, Pham Van M, Favreau Amanda J, Barbian Kent D, Sanchez-Vargas Irma, Calvo Eric, Ribeiro José MC

“…The average amino acid identity among salivary proteins is 70.1%, while that for housekeeping proteins is 91.2% (P < 0.05), and the codon volatility of secreted proteins is significantly higher than those of housekeeping proteins. …”
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Forward genetic studies reveal LsAPRR2 as a key gene in regulating the green color of pericarp in bottle gourd (Lagenaria siceraria) by Yulai Huo, Gui Zhang, Wenjin Yu, Zhengguo Liu, Mujie Shen, Rongchong Zhao, Shengping Hu, Xuyang Zheng, Peng Wang, Yanjuan Yang

“…Cloning and sequence comparison of the two parental LsAPRR2 promoter regions indicated 11 bases insertion and 8 SNPs mutations in the region -991~-1033, upstream of the start codon in white bottle gourd. Proof of GUS reporting system, Genetic variation in this fragment significantly reduced the expression of LsAPRR2 in the pericarp of white bottle gourd. …”
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16-membered ring macrolides and erythromycin induce ermB expression by different mechanisms by Weizhi He, Kai Jiang, Hua Qiu, Lijun Liao, Shasha Wang

“…Abstract Background Ribosome stalling on ermBL at the tenth codon (Asp) and mRNA stabilization are believed to be mechanisms by which erythromycin (Ery) induces ermB expression. …”
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IgA Nephropathy Associated with IgA Vasculitis Coexisting with Focal Segmental Glomerulosclerosis Tip Variant by Zitlali Guadalupe Paulín Zepeda, Karla Daniela Salgado Guizar, Vianey Guadalupe Tellez Bolaños, Louis Fernando Robles Fernandes, María Inés Gil Arredondo

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The Complete Mitochondrial Genome of Four Hylicinae (Hemiptera: Cicadellidae): Structural Features and Phylogenetic Implications by Jiu Tang, Weijian Huang, Yalin Zhang

“…Among 13 protein-coding genes, most show typical ATN start codons and TAR (TAA/TAG) or an incomplete stop codon T–, and several genes start by TTG/GTG. …”
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A dynamic RNA loop in an IRES affects multiple steps of elongation factor-mediated translation initiation by Marisa D Ruehle, Haibo Zhang, Ryan M Sheridan, Somdeb Mitra, Yuanwei Chen, Ruben L Gonzalez Jr, Barry S Cooperman, Jeffrey S Kieft

“…These IRESs require an RNA pseudoknot that mimics a codon-anticodon interaction and contains a conformationally dynamic loop. …”
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TERTmonitor—qPCR Detection of <i>TERTp</i> Mutations in Glioma by João Paulo Brás, Tito Teles Jesus, Hugo Prazeres, Jorge Lima, Paula Soares, João Vinagre

“…Telomerase promoter (TERTp) mutations are frequently observed in various types of tumours and commonly characterised by two specific hotspots located at positions −124 and −146 upstream of the start codon. They enhance <i>TERTp</i> activity, resulting in increased TERT expression. …”
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Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei by Shanshan Jiang, Yue Wang, Qin Liu, Qinqin Zhao, Liwei Gao, Xin Song, Xuezhi Li, Yinbo Qu, Guodong Liu

“…Here, we identified that the region 600- to 700-bp upstream of the start codon is critical for the efficiency of the cdna1 promoter. …”
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Identification of H-2d Restricted T Cell Epitope of Foot-and-mouth Disease Virus Structural Protein VP1 by Zhang Zhong-Wang, Zhou Peng, Lu Jian-Liang, Pan Li, Fang Yu-Zhen, Zhang Yong-Guang, Wang Yong-Lu, Liu Xin-Sheng, Jiang Shou-Tian

“…</p> <p>Results</p> <p>A codon-optimized expression method was adopted for effective expression of VP1 protein in colon bacillus. …”
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Antisense Oligonucleotide-Mediated Splice Switching: Potential Therapeutic Approach for Cancer Mitigation by Prithi Raguraman, Akilandeswari Ashwini Balachandran, Suxiang Chen, Sarah D. Diermeier, Rakesh N. Veedu

“…AOs can also be used to induce splice switching, where the expression of an oncogenic protein can be inhibited by the induction of a premature stop codon. In general, AOs are modified chemically to increase their stability and binding affinity. …”
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Sustainable recovery of MBNL activity in autoregulatory feedback loop in myotonic dystrophy by Zuzanna Rogalska, Krzysztof Sobczak

“…The genetic construct contains an MBNL1-coding sequence separated by the fragment of ATP2A1 pre-mRNA with an MBNL-sensitive alternative exon containing stop codon in the reading frame of MBNL1. Inclusion of this exon leads to the arrangement of an inactive form of the protein, but exclusion gives rise to fully active MBNL1. …”
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Application of reverse vaccinology to design a multi-epitope subunit vaccine against a new strain of Aeromonas veronii by Sk Injamamul Islam, Moslema Jahan Mou, Saloa Sanjida

“…Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher CAI value, which was then included in the cloning vector pET2+ (a). …”
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