STAT3 Relays a Differential Response to Melanoma-Associated <i>NRAS</i> Mutations by James Kim, Daniel Novak, Christos Sachpekidis, Jochen Utikal, Lionel Larribère

“…Here, we investigated the influence of different melanoma-associated <i>NRAS</i> mutations (codon 12, 13 or 61) on several parameters such as oncogene-induced senescence, cell proliferation, migration or colony formation in immortalized melanocytes and in melanoma cell lines. …”
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Identification of a novel ANK1 gene variant c.1504-9G>A and its mechanism of intron retention in hereditary spherocytosis by Ting Xiong, Zhongjin Xu, Qian Wan, Feng Chen, Yao Ye, Hong Wang, Chongjun Wu

“…This variant results in a premature stop codon and the production of a truncated protein. …”
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Nuclear mRNA Quality Control and Cytoplasmic NMD Are Linked by the Guard Proteins Gbp2 and Hrb1 by Yen-Yun Lu, Heike Krebber

“…This “memory” of splicing events is important for the ribosome, which translates the RNAs in the cytoplasm. In case a stop codon was detected before an EJC, translation is blocked and the RNA is eliminated by the nonsense-mediated decay (NMD). …”
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Impressive and Prolonged Response with Lenvatinib in a Highly Pretreated Patient with Metastatic Clear Cell Renal Cancer: A Case Report by Sara Demurtas, Mara Frascaroli, Federico Sottotetti, Alessandro Rametta, Giuseppe Procopio, Laura Deborah Locati

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Generation of human induced pluripotent stem cell line (MUi034-A) from an unusual case of hydrops fetalis associated with homozygous hemoglobin Constant Spring by Wasinee Wongkummool, Pirut Tong-ngam, Thongperm Munkongdee, Amornrat Tangprasittipap, Kittiphong Paiboonsukwong, Suradej Hongeng, Suthat Fucharoen, Pimlak Charoenkwan, Alisa Tubsuwan

“…Hemoglobin Constant Spring (HbCS) is unstable hemoglobin resulting from a nucleotide substitution at the termination codon of the HBA2 gene (c.427 T > C). The homozygous state for HbCS is non-transfusion dependent in adults. …”
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BRAF Mutations in Melanoma: Biological Aspects, Therapeutic Implications, and Circulating Biomarkers by Giorgia Castellani, Mariachiara Buccarelli, Maria Beatrice Arasi, Stefania Rossi, Maria Elena Pisanu, Maria Bellenghi, Carla Lintas, Claudio Tabolacci

“…The most frequent oncogenic BRAF mutations consist of a single point mutation at codon 600 (mostly V600E) that leads to constitutive activation of the BRAF/MEK/ERK (MAPK) signalling pathway. …”
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Generation of an induced pluripotent stem cell line from a patient with hereditary multiple endocrine neoplasia 2A (MEN2A) syndrome with RET mutation by J. Hadoux, O. Féraud, F. Griscelli, P. Opolon, D. Divers, E. Gobbo, M. Schlumberger, A. Bennaceur-Griscelli, A.G. Turhan

“…We generated induced pluripotent stem cells (iPSCs) from a patient with RET mutation at codon 634 who developed pheochromocytoma and MTC. …”
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Localization of Pyranose 2-Oxidase from <i>Kitasatospora aureofaciens</i>: A Step Closer to Elucidate a Biological Role by Ludovika Jessica Virginia, Clemens Peterbauer

“…Analysis of the genomic locus of POX from <i>Kitasatospora aureofaciens</i> (<i>Ka</i>POx), which is similar to fungal POx, revealed a start codon upstream of the originally annotated one, and the additional sequence was considered a putative Tat-signal peptide by computational analysis. …”
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<i>NSDHL</i> Frameshift Deletion in a Mixed Breed Dog with Progressive Epidermal Nevi by Matthias Christen, Michaela Austel, Frane Banovic, Vidhya Jagannathan, Tosso Leeb

“…The variant is predicted to produce a premature stop codon truncating 34% of the encoded protein, p.Glu240Profs*17. …”
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Upstream Open Reading Frame Mediated Translation of WNK8 Is Required for ABA Response in <i>Arabidopsis</i> by Zhiyong Li, Yajuan Fu, Jinyu Shen, Jiansheng Liang

“…Plants transformed with the native <i>WNK8</i> promoter driving WNK8 expression were comparable with wild-type plants, whereas the plants transformed with a similar construct with mutated CPuORF58 start codon were less sensitive to ABA. In addition, WNK8 and its downstream target RACK1 were found to synergistically coordinate ABA signaling rather than antagonistically modulating glucose response and flowering in plants. …”
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Role of Serine 310 in the Activity and Temperature Profile of Human Caspase-9 by Raheleh Shakeri, Mohadeseh Mahmoudian, Reza Khodarahmi, Soheila Mohammadi

“…Materials and Methods: Considering that phosphorylation leads to negative charge on caspase-9, the codon of serine 310 in human capsase-9 was mutated to aspartate via quick change site-directed mutagenesis. …”
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Optimization of Expression Conditions, Purification and Identification of the Recombinant Cowpea Hemoglobin Lb Ⅱ in Escherichia coli by Meng LIU, Congrui WANG, Bo LIU, Xiangzhong ZHAO

“…Using the pET-15b as the expression vector and E. coli BL21-CodonPlus (DE3)-R-IL as the recombinant expression host, the cowpea Lb Ⅱ was successfully expressed, then preliminarily purified this protein by using the nickel column chromatography and added ascorbic acid as the antioxidant simultaneously. …”
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Changing colors and understanding: the use of mutant chromogenic protein and informational suppressor strains of Escherichia coli to explore the central dogma of molecular biology by Sarah DeWolf, Maartje Van den Bogaard, Rachael Brady Hart, Sparrow Hartman, Nancy Boury, Gregory J. Phillips

“…The students introduce a plasmid carrying a nonfunctional chromogenic protein gene due to a nonsense mutation in a codon encoding the chromophore to various nonsense suppressor strains of Escherichia coli. …”
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Low‐grade serous carcinoma detected from intraoperative peritoneal washings: Cytological findings and detection of KRAS mutation by Eriko Yamamoto, Kenji Warigaya, Yuichi Kinoshita, Ayana Yamamoto, Shin‐ichi Murata

“…The genetic analysis that was done using exfoliated cells from peritoneal washings specimen by idensy™, detected KRAS mutation at codon 12/13. Conclusion The genetic investigation using cytological specimen as well as characteristic cytological findings were useful to make differential diagnosis between LGSC and HGSC.…”
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Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins by Ying Ma, Hernán Biava, Roberto Contestabile, Nediljko Budisa, Martino Luigi di Salvo

“…In particular, we engineered the intracellular biosynthesis of L-azidohomoalanine from O-acetyl-L-homoserine and NaN3, and achieved its direct incorporation into recombinant target proteins by AUG codon reassignment in a methionine-auxotroph E. coli strain. …”
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<i>Ustilago maydis</i> Metabolic Characterization and Growth Quantification with a Genome-Scale Metabolic Model by Ulf W. Liebal, Lena Ullmann, Christian Lieven, Philipp Kohl, Daniel Wibberg, Thiemo Zambanini, Lars M. Blank

“…Here, we report the first genome-scale metabolic model (GSMM) of <i>Ustilago maydis</i> (iUma22) for the simulation of metabolic activities. iUma22 was reconstructed from sequencing and annotation using PathwayTools, and the biomass equation was derived from literature values and from the codon composition. The final model contains over 25% annotated genes (6909) in the sequenced genome. …”
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Cloning and Expression of Domain III of Dengue Virus Type 2 Envelope Protein in Escherichia Coli by Hossein Fahimi, Majid Sadeghizadeh, Mahshid Mohammadipoor

“…For high expression level, the sequence of EDIII2 gene was optimized for appropriate codon usage and GC content. The coding gene sequence was approved by sequencing and enzymatic digestion. …”
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Analysis of genetic variants in the exon 2 and 3 of autosomal DAZLA gene among infertile South Indian men by Puja Devi Nongthombam, Suttur S. Malini

“…We also discovered two new deleterious genetic variations in exon 3 of the DAZLA gene, one nonsynonymous mutation that replaced Valine with Glutamate at the 66 codon position and the other a stop gain mutation at the 74 amino acid position. …”
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Active Surveillance of Small Renal Masses in a Large Danish Cohort: Assessing Proficiency in Patient Selection by Rasmine Bak, Jørgen Bjerregaard Jensen, Tau Pelant, Rikke Nørresø Haase, Tommy Kjærgaard Nielsen

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Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumul... by Dana Kolibachuk, Benjamin J. Ryder, Edward R. Lyons, Ariel Woolsey, Margaret K. Lopes, Keya Thakkar, Mayelin Pacheco Nunez, Michaela Duquenoy, Nevan R. Valente, Ashley Nieves

“…A mutational analysis confirmed the identity of the start codon for the PHA synthase gene and provided evidence supporting the requirement for phasins to allow for PHA accumulation in the recombinant hosts. …”
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