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2741
Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB
Published 2017-09-01“…In this work, we have expressed synthetic codon-optimized versions of NifB from the γ-proteobacterium Azotobacter vinelandii and the thermophilic methanogen Methanocaldococcus infernus in Saccharomyces cerevisiae and in Nicotiana benthamiana. …”
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2742
Genome-Wide Transcription Start Sites Mapping in <i>Methylorubrum</i> Grown with Dichloromethane and Methanol
Published 2022-06-01“…One-third of the detected TSSs were located at a distance to the start codon inferior to 250 nt (average of 84 nt) with 7% of leaderless mRNA. …”
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2743
Score-based prediction of genomic islands in prokaryotic genomes using hidden Markov models
Published 2006-03-01“…It is based on the analysis of codon usage (CU) of each individual gene of a genome under study. …”
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2744
Mutation in grlA and norA genes in Fluoroquinolone resistant strains of S. aureus
Published 2017-03-01“…The obtained results demonstrated that grlA gene mutation is one of the most important mechanisms of resistance to ciprofloxacin in clinical isolates of S. aureus in Rasht and most of resistant isolates acquired common mutation in codon 80. Key Words: Fluoroquinolone, S. aureus, grlA , norA…”
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2745
High resolution melting analysis for rapid and sensitive <it>EGFR </it>and <it>KRAS </it>mutation detection in formalin fixed paraffin embedded biopsies
Published 2008-05-01“…Sequencing of these 25 samples confirmed the presence of codon 12 or 13 mutations. <it>EGFR </it>and <it>KRAS </it>mutations were mutually exclusive.…”
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2746
High Levels of PD-L1+ and Hyal2+ Myeloid-derived Suppressor Cells in Renal Cell Carcinoma
Published 2022-04-01Get full text
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2747
Molecular Basis and Natural History of Medullary Thyroid Cancer: It is (Almost) All in the <i>RET</i>
Published 2023-10-01“…The initial theory of <i>RET</i> codon-related MTC aggressiveness has been recently questioned by studies suggesting that this would only define the age at disease onset rather than the aggressiveness of MTC. …”
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2748
In-silico design of an immunoinformatics based multi-epitope vaccine against Leishmania donovani
Published 2022-08-01“…In-silico immune simulation studies confirms the vaccine to raise a pro-inflammatory response with the proliferation of activated T and B cells. In-silico codon optimization and cloning of the vaccine nucleic acid sequence have also been achieved in the pET28a vector. …”
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2749
Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous cell carcinoma.
Published 2013-01-01“…EGFR and RASSF1A expression was evaluated by immunohistochemistry. KRAS mutations at codons 12 and 13, and the BRAF mutation at codon 600 were analyzed on DNA isolated from formalin fixed paraffin embedded tissues by direct genomic sequencing. …”
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2750
Comparative mitogenomics and phylogenetic analyses of the genus Menida (Hemiptera, Heteroptera, Pentatomidae)
Published 2023-01-01“…The results showed that gene arrangements, nucleotide composition, codon preference, gene overlaps, and RNA secondary structures were highly conserved within the Menida and had more similar characteristics in Pentatominae. …”
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2751
Complete mitochondrial genome of Hygrobates turcicus Pešić, Esen & Dabert, 2017 (Acari, Hydrachnidia, Hygrobatoidea)
Published 2022-12-01“…Five genes have alternative start-codon, and four display premature termination. The multigene phylogeny obtained using all mitochondrial protein-coding genes unambiguously associates H. turcicus with the cluster formed by H. longiporus and H. taniguchii.…”
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2752
Massive‐scale genomic analysis reveals SARS‐CoV‐2 mutation characteristics and evolutionary trends
Published 2022-09-01“…Furthermore, we hypothesized that translation efficiency can affect the mutation frequency of the third position of the codon by the selection, which explains why SARS‐CoV‐2 prefers AT3 codons usage. …”
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2753
Iron in Translation: From the Beginning to the End
Published 2021-05-01“…The influence of these modifications and their correlation with codon bias in the dynamic control of protein biosynthesis, mainly in response to stress, is emerging as an interesting focus of research. …”
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2754
Enhanced Production of a Thermostable Carbonic Anhydrase in <i>Escherichia coli</i> by Using a Modified NEXT Tag
Published 2021-09-01“…The expression test with all 16 codon combinations for the ng3 sequence revealed that the change in translation initiation rate brought about by the change in nucleotide sequence was not the primary determinant for the change in expression level. …”
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2755
Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli
Published 2022-10-01“…However, non-optimised codons and expression at 30 °C gave the highest activity through improved protein quality, with increased k cat and T m values. …”
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2756
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2757
Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR- overexpressing cancer cells
Published 2018-04-01“…To this end, CDR loops of cetuximab (an FDA-approved anti-EGFR antibody) were grafted on framework regions derived from type 3 (VH3 and VL3 kappa) human germline sequences to obtain recombinant VH and VL domainslinked together with a flexible linker [(Gly4Ser)3] to form a scFv. Codon optimized synthetic gene encoding the scFv (with NH2-VH-linker-VL-COOH orientation) was expressed in E. coli Origami™ 2(DE3) cells and the resultant scFv purified by using Ni-NTA affinity chromatography. …”
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2758
MicroSalmon: A Comprehensive, Searchable Resource of Predicted MicroRNA Targets and 3′UTR Cis-Regulatory Elements in the Full-Length Sequenced Atlantic Salmon Transcriptome
Published 2021-09-01“…Many of these were enriched in the terminal end, while purine- or pyrimidine-rich motifs with unknown functions were enriched immediately downstream of the stop codon. Furthermore, several novel complex motifs were over-represented, indicating conservation and putative function. …”
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2759
Advancing Treatment Frontiers: Radiofrequency Ablation for Small Renal Mass—Intermediate-Term Results
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2760
Immunoinformatics design of multi-epitope vaccine using OmpA, OmpD and enterotoxin against non-typhoidal salmonellosis
Published 2023-02-01“…The vaccine model had good binding affinity and stability with MHC-I, MHC-II, and toll-like receptors (TLR-1, 2, 4) as well as activation of T cells, IgM, IgG, IFN- $$\gamma$$ γ and IL-2 responses. Furthermore, after codon optimization of the vaccine sequence, this sequence was cloned in E. coli plasmid vector pET-30a (+) within restriction sites of HindIII and BamHI. …”
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