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2781
Comprehensive Analysis of the Transcriptome-Wide m6A Methylation in Mouse Pachytene Spermatocytes and Round Spermatids
Published 2022-03-01“…The peaks were mainly concentrated in the coding region and the stop codon of the GGAC motif. In addition, enrichment analysis showed significant m6A methylation genes in related pathways in spermatogenesis. …”
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2782
Comparative Plastomics of Ashwagandha (<i>Withania</i>, Solanaceae) and Identification of Mutational Hotspots for Barcoding Medicinal Plants
Published 2020-06-01“…We analyzed the structural organization, gene content and order, guanine-cytosine content, codon usage, RNA-editing sites, microsatellites, oligonucleotide and tandem repeats, and substitutions of <i>Withania</i> plastomes, which revealed high similarities among the species. …”
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2783
Non-host class II ribonucleotide reductase in Thermus viruses: sequence adaptation and host interaction
Published 2019-04-01“…Phylogenetic analysis revealed a distant origin of the nrdJm gene with the most closely related sequences found in mesophiles or moderate thermophiles from the Firmicutes phylum. GC-content, codon usage and the ratio of coding to non-coding substitutions (dN/dS) suggest extensive adaptation of the gene in the virus in terms of nucleotide composition and amino acid sequence. …”
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2784
Cloning and Expression of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica)
Published 2009-01-01“…It contains putative selenocysteine residue which is encoded by an unusual stop codon TGA. Comparison of amino acid sequences showed that Japanese quail GPX1 shares 65-74% identity with GPX1 of other animals, suggesting that our clone is an authentic member of GPX1. …”
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2785
In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activity
Published 2022-01-01“…This study aimed to produce epitopes of CCL21 and IL1β as a recombinant protein and characterize its in vitro anti-tumor and immunogenic activity. A codon-optimized ccl21/IL1β gene was designed and synthesized from human genes. …”
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2786
Hepatitis C Virus Translation Regulation
Published 2020-03-01“…Moreover, some ITAFs can act as RNA chaperones that help to position the viral AUG start codon in the ribosomal 40S subunit entry channel. …”
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2787
Clinicopathologic Spectrum of Nephrotic Syndrome in Elderly
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2788
The Asia 2 specific signal peptide region and other domains in fusion protein genes characterized Asia 1 and Asia 2 canine distemper viruses
Published 2009-10-01“…</p> <p>Results</p> <p>The phylogenetic analysis of F and H gene sequences from fourteen CDV isolates obtained from diseased dogs in Japan and Thailand indicated that the F genes had a new initiation codon and extra 27 nucleotides upstream of the usual open reading frame (ORF) and the F proteins had extra 9 amino acids at the N-terminal position only in Asia 2 isolates. …”
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2789
Full-length transcriptome characterization and comparative analysis of Gleditsia sinensis
Published 2023-12-01“…AGA, AGG, and CCA were identified as the universal optimal codon in the genus of Gleditsia. EIF5A was selected as a suitable fluorescent quantitative reference gene. 315 Cytochrome P450 monooxygenases (CYP450s) and 147 uridine diphosphate (UDP)-glycosyltransferases (UGTs) were recognized through the PacBio SMRT transcriptome. …”
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2790
Case report: FOXP1 syndrome caused by a de novo splicing variant (c.1652+5 G>A) of the FOXP1 gene
Published 2022-08-01“…Assessed by TA-cloning, the variant promoted the skipping of exon 18 and a premature stop codon (p.Asn511*), resulting in a predicted truncated protein. …”
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2791
Mutation Detection in MYO15A Gene in an Iranian Family with Non-Syndromic Hearing Loss
Published 2024-03-01“…The newly identified mutation, causing a new reading frame (p.Tyr393Serfs*38), results in an early encounter with a stop codon, leading to the formation of a shortened protein. …”
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2792
Unusual presentation of hepatitis B serological markers in an Amerindian community of Venezuela with a majority of occult cases
Published 2011-12-01“…One isolate exhibited the co-circulation of a wild type variant with a variant with a premature stop codon at the core protein, and a variant exhibiting a deletion of 28 amino acids.…”
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2793
Pacing Dynamics Determines the Arrhythmogenic Mechanism of the CPVT2-Causing CASQ2<sup>G112+5X</sup> Mutation in a Guinea Pig Ventricular Myocyte Computational Model
Published 2022-12-01“…CASQ2<sup>G112+5X</sup> is a 16 bp (339–354) deletion CASQ2 mutation that prevents the protein expression due to premature stop codon. Understanding the subcellular mechanisms of CPVT2 is experimentally challenging because the occurrence of arrhythmia is rare. …”
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2794
Engineered bacterial voltage-gated sodium channel platform for cardiac gene therapy
Published 2022-02-01“…Here, we report a platform utilizing small-size, codon-optimized engineered prokaryotic sodium channels (BacNav) driven by muscle-specific promoters that significantly enhance excitability and conduction in rat and human cardiomyocytes in vitro and adult cardiac tissues from multiple species in silico. …”
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2795
The Complete Mitochondrial Genomes of Four Species in the Subfamily Limenitidinae (Lepidoptera, Nymphalidae) and a Phylogenetic Analysis
Published 2021-12-01“…All four mitogenomes display similar nucleotide content and codon usage of protein-coding genes (PCGs). Typical cloverleaf secondary structures are identified in 21 tRNA genes, while <i>trnS1</i> (AGN) lacks the dihydrouridine (DHC) arm. …”
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2796
Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
Published 2016-01-01“…To illustrate the scarless N- and C-terminal gene tagging methods in S. cerevisiae, we tagged various genes including the E3 ubiquitin ligase RSP5, the proteasome subunit PRE1, and the eleven Rab GTPases with yeast codon-optimized mNeonGreen or mCherry; several of these represent essential genes. …”
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2797
Alternative Splicing of the Aflatoxin-Associated Baeyer–Villiger Monooxygenase from <i>Aspergillus flavus</i>: Characterisation of MoxY Isoforms
Published 2018-12-01“…Using mRNA data, an intron near the C-terminus was identified that is alternatively spliced, creating two possible MoxY isoforms which exist in vivo, while analysis of the genomic DNA suggests an alternative start codon leading to possible elongation of the N-terminus. …”
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2798
Metatranscriptomic analysis uncovers prevalent viral ORFs compatible with mitochondrial translation
Published 2023-06-01“…We exploit this genetic diversity to identify new clades of Mitoviridae, annotate clade-specific sequence motifs that distinguish the mitoviral RdRp, and reveal patterns of RdRp codon usage consistent with translation on host cell mitoribosomes. …”
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2799
Complete chloroplast genome sequence of Rhododendron mariesii and comparative genomics of related species in the family Ericaeae
Published 2023-08-01“…Leucine was the most representative amino acid, while cysteine was the lowest representative. Totally, 30 codons showed obvious codon usage bias, and most were A/U-ending codons. …”
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2800
Characterization and Comparison of the Two Mitochondrial Genomes in the Genus <i>Rana</i>
Published 2023-09-01“…The mitogenomes of both species contained 37 genes, including 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA genes, and one control region; mitogenome size varied predominantly with the length of the control region. The two synonymous codon usages in 13 PCGs showed that T and A were used more frequently than G and C. …”
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