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2921
Pectin acetylesterase 8 influences pectin acetylation in the seed coat, seed imbibition, and dormancy in common bean (Phaseolus vulgaris L.)
Published 2022-09-01“…Genomic sequence data identified a non‐functional allele, due to a five‐base pair insertion resulting in a frameshift and premature stop codon. The non‐functional pae8 allele was associated with a lack of detectable protein as determined by Western blot. …”
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2922
Viral proteogenomic and expression profiling during productive replication of a skin-tropic herpesvirus in the natural host.
Published 2023-06-01“…We identified peptides representing alternative start codon usage in several genes and putative novel microORFs at the 5' ends of two core herpesviral genes, pUL47 and ICP4, along with strong evidence of independent transcription and translation of the capsid scaffold protein pUL26.5. …”
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2923
Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters
Published 2018-12-01“…To improve the heterologous expression of BGCs, herein we optimized S. lividans strain SBT5 via the stepwise integration of three global regulatory genes and two codon-optimized multi-drug efflux pump genes and deletion of a negative regulatory gene, yielding four engineered strains. …”
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2924
RSV Vaccine Based on Rhabdoviral Vector Protects after Single Immunization
Published 2019-07-01“…Wild-type F (F<sub>wt</sub>) or a codon optimized version (F<sub>syn</sub>) were introduced at position 5 into the VSV-GP genome. …”
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2925
Comparison evaluation between gene Xpert Mtb/Rif and multiplex PCR for rapid diagnosis of mycobacterium tuberculosis
Published 2020-11-01“…Multiplex PCR detected only 44 RIF culture resistant, while remaining 40 did not showed resistance to rpoB gene codon 531 region. Sensitivity, Specificity, PPV and NPV of Gene Xpert MTB/RIF were 100% each respectively. …”
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2926
PoSE: visualization of patterns of sequence evolution using PAML and MATLAB
Published 2018-10-01“…Substitutions are displayed by category (transitions and transversions), codon position, and phenotypic effect (synonymous and nonsynonymous). …”
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2927
Complete mitochondrial genome of Rectoris luxiensis (Teleostei, Cyprinidae): characterisation and phylogenetic implications
Published 2023-01-01“…All the 13 PCGs were found to start with ATG codons, except for the COXI, in which GTG was the start codon. …”
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2928
Intraoperative Embolization during Inferior Vena Cava Tumor Thrombectomy for Renal Cell Carcinoma
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2929
Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3)
Published 2019-10-01“…As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. …”
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2930
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2931
Biochemical Atypia in Russian <i>Neisseria gonorrhoeae</i> Clinical Isolates Belonging to the G807 NG-MAST Genogroup/ST1594 MLST
Published 2022-11-01“…Cultures with defective amino acid metabolism had a 5-nucleotide insertion in the <i>pip</i>-gene that caused a stop codon and led to synthesis of the non-functional enzyme. …”
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2932
Genome-wide identification and analysis of B-box zinc finger gene family in sugarcane (Saccharum officinarum)
Published 2023-08-01“…Results: Considered in the present research work, 21 Sugarcane genes were discovered by comprehensive bioinformatics analysis viz the phylogeny relationships, structures of genes, locations of chromosomes, gene duplication, subcellular localization, homology and codon analysis were analysed. Relying on phylogeny as well as domain constitution analysis, the identified genes must be divided into five clades. …”
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2933
The Barley Chloroplast Mutator (<i>cpm</i>) Mutant: All Roads Lead to the <i>Msh</i>1 Gene
Published 2022-02-01“…A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the <i>Msh</i>1 gene of <i>cpm</i> plants. …”
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2934
Comparative Analysis of Mitochondrial Genomes between the B-Type Cytoplasmic Male Sterility Line and Its Maintainer Line in Wheat
Published 2022-03-01“…The B and YS9 genomes were assembled into a typical circular molecule 452,794 and 452,453 bp in length, respectively, comprising 34 protein-coding genes (PCGs), 3 ribosomal RNA genes (rRNAs), and 16 transfer RNA genes (tRNAs). The codon usage analysis revealed leucine (Leu) and serine (Ser) as the most frequently used amino acid residues in the B and YS9 mitochondrial proteins. …”
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2935
Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas
Published 2024-02-01“…These consensus sequences were used to design Pseudomonas codon-optimized genes named psc1 and psd1 and assays were conducted to test the activity in P. putida. …”
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2936
Detection of Mutations in Precore and Basal Core Region in HBeAg Negative Chronic Hepatitis B Patients from Jaipur
Published 2023-06-01“…G1896A, which is the most frequently observed mutation in the PC region, acts by insertion of a premature stop codon in the concerned ORF while the A1762T/G1764A double mutation in the BCP region acts by termination of HBeAg expression. …”
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2937
Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome.
Published 2018-02-01“…We generated EBNA-LP knockout (LPKO) EBVs containing a STOP codon within each repeat unit of internal repeat 1 (IR1). …”
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2938
TusDCB, a sulfur transferase complex involved in tRNA modification, contributes to UPEC pathogenicity
Published 2024-04-01“…Abstract tRNA modifications play a crucial role in ensuring accurate codon recognition and optimizing translation levels. …”
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2939
Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in <i>Pichia pastoris</i>
Published 2023-02-01“…In this study, the carboxylesterase FumDM from <i>Sphingopyxis</i> sp. ASAG22 was codon-optimized and co-expressed with five different molecular chaperones (PDI, CPR5, ERO1, HAC1, and Bip) in order to improve the expression level of FumDM in <i>Pichia pastoris</i> (also known as <i>Komagataella phaffii</i>) GS115. …”
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2940
Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries
Published 2016-06-01“…We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. …”
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