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2961
RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering of Kluyveromyces marxianus
Published 2022-12-01“…The use of a strong RNA polymerase II promoter allows efficient expression of gRNAs flanked by the self-cleaving RNA structures, tRNA and HDV ribozyme, from a single plasmid co-expressing a codon optimized Cas9. Implementing this system resulted in nearly 100% efficiency of gene disruptions in both NHEJ-functional and NHEJ-deficient K. marxianus strains, with donor integration efficiencies reaching 50% and 100% in the two strains, respectively. …”
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2962
Comparative Mitogenomic Analysis of Five Awl Skippers (Lepidoptera: Hesperiidae: Coeliadinae) and Their Phylogenetic Implications
Published 2021-08-01“…Most protein-coding genes (PCGs) begin with ATN, with 3 types of stop codons including TAA, TAG, and an incomplete codon T-; most of the genes terminate with TAA. …”
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2963
Mitochondrial genome of Chrysochares punctatus (Coleoptera: Chrysomelidae: Eumolpinae) and phylogenetic analysis
Published 2020-01-01“…Ten PCGs started with a typical ATN codon, whereas the remaining three PCGs started with AAC (cox1) and TTG (nad1 and nad2). …”
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2964
Generation of an induced pluripotent stem cell line (IGGi002A) from nasal cells of a cystic fibrosis patient homozygous for the G542X-CFTR mutation
Published 2023-10-01“…Several CFTR mutations are causative of CF, among which G542X is a nonsense mutation introducing a premature stop codon which prevents CFTR protein synthesis. We generated a new iPSC line from nasal cells carrying G542X homozygous mutation for CFTR: IGGi002A. …”
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2965
Comparative Mitogenomic Analysis of Two Cuckoo Bees (Apoidea: Anthophila: Megachilidae) with Phylogenetic Implications
Published 2021-01-01“…The obtained results show that 37 mitogenomic genes and one putative control region were conserved within Hymenoptera. Truncated stop codon T was found in the <i>cox3</i> gene of <i>E. polynesia</i>. …”
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2966
Cytotoxic Mechanism of Excess Polyamines Functions through Translational Repression of Specific Proteins Encoded by Polyamine Modulon
Published 2020-03-01“…It was suggested that the structure of the mRNA bulged-out region is affected by excess spermidine, so the SD sequence of the <i>rmf</i> mRNA cannot approach initiation codon AUG.…”
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2967
A Case with Laron Syndrome
Published 2019-07-01“…A missense variation leading to a stop codon (W182X) was determined in GH receptor gene. …”
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2968
Design, Expression and Evaluation of Bacteriostatic Activity of Hybrid Antimicrobial Peptide NK-LPd
Published 2023-09-01“…The hybrid antimicrobial peptide NK-LPd gene was optimized according to Pichia pastoris codon preference principle. The optimized gene was amplified by gene splicing via overlap extension polymerase chain reaction (gene splicing by overlap extension polymerase chain reaction, SOE-PCR) and inserted into the secretory expression vector pPIC9K. …”
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2969
Identification and Characterization of a New Splicing Variant of Prokineticin 2
Published 2022-02-01“…In addition, the introduction of the unnatural amino acid p-benzoyl-L-phenylalanine using amber codon suppression technology demonstrated the role of tryptophan at position 212 of PROKR2 for PROK2C binding by photoactivatable cross-linking. …”
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2970
Characteristics of the Patients Aged Less than 40 Years Operated for a Renal Mass
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2971
Integrated genomic analyses of acral and mucosal melanomas nominate novel driver genes
Published 2022-06-01“…Amplifications of PAK1 and GAB2 were more commonly observed in acral melanomas, whereas SF3B1 R625 codon mutations were unique to mucosal melanomas (12.9%). …”
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2972
Amino Acids 563–566 of the Na<sup>+</sup>/H<sup>+</sup> Exchanger Isoform 1 C-Terminal Cytosolic Tail Prevent Protein Degradation and Stabilize Protein Expression and Activity
Published 2020-03-01“…A previous study demonstrated that stop codon polymorphisms can result in decreased activity, expression, targeting and enhanced protein degradation. …”
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2973
Molecular epidemiology of potential candidate markers for chloroquine resistance in imported Plasmodium vivax malaria cases in Iran
Published 2023-04-01“…Results None of the samples had any mutation at codon 976 of pvmdr1, while the 1076 mutation was detected in 96.2% of the examined isolates. …”
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2974
Novel compound heterozygous variants in EMC1 associated with global developmental delay: a lesson from a non-silent synonymous exonic mutation
Published 2023-04-01“…RT-PCR assay followed by Sanger sequencing reveals that the c.2376G>A variant leads to aberrant splicing, with retention of intron 19 (561bp) in the mature mRNA, which is presumed to introduce a premature translational termination codon (p.(Val792fsTer31)).ConclusionNovel compound heterozygous variants in EMC1 have been identified in individuals with global developmental delay. …”
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2975
Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis
Published 2023-01-01“…Eukaryotic translation initiation factor 5A (eIF5A), upon being hypusinated, is essential for the translation elongation of proline-codon rich mRNAs. In this study, we found that increased hypusinated eIF5A protein levels were associated with cardiac fibrosis and heart dysfunction in myocardial infarction (MI) mouse models. …”
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2976
Coordination of m<sup>6</sup>A mRNA Methylation and Gene Transcriptome in Sugarcane Response to Drought Stress
Published 2023-10-01“…In this study, m<sup>6</sup>A sites of sugarcane transcriptome were significantly enriched around the stop codon and within 3′-untranslated regions (3′UTR). …”
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2977
Genome sequence analysis suggests coevolution of the DIS, SD, and Psi hairpins in HIV-1 genomes
Published 2022-11-01“…We supposed that the G-quadruplex, located 56 nts downstream of the Gag start codon, may participate in switching of HIV-1 leader RNA from BMH (branched multiple hairpins) to LDI (long distance interaction) conformation.…”
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2978
Korean Red Ginseng increases defective pol gene in peripheral blood mononuclear cells of HIV-1–infected patients; inhibition of its detection during ginseng-based combination thera...
Published 2019-10-01“…In contrast, the proportion of stop codon appeared not to be affected by KRG treatment. …”
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2979
Genetic Code Expansion System for Tight Control of Gene Expression in <i>Bombyx mori</i> Cell Lines
Published 2021-12-01“…In D[TAG]G, the amber stop codon is recognized as the orthogonal tRNA<sup>Pyl</sup>. …”
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2980
Mitochondrial genome variation and intergenomic sequence transfers in Hevea species
Published 2024-04-01“…The four mitochondrial genomes had consistent GC contents, codon usages and AT skews. However, there were significant differences in the genome lengths and sequence repeats. …”
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