RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering of Kluyveromyces marxianus by Danielle Bever, Ian Wheeldon, Nancy Da Silva

“…The use of a strong RNA polymerase II promoter allows efficient expression of gRNAs flanked by the self-cleaving RNA structures, tRNA and HDV ribozyme, from a single plasmid co-expressing a codon optimized Cas9. Implementing this system resulted in nearly 100% efficiency of gene disruptions in both NHEJ-functional and NHEJ-deficient K. marxianus strains, with donor integration efficiencies reaching 50% and 100% in the two strains, respectively. …”
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Comparative Mitogenomic Analysis of Five Awl Skippers (Lepidoptera: Hesperiidae: Coeliadinae) and Their Phylogenetic Implications by Qi Sun, Yumeng Yang, Xiangyu Hao, Jintian Xiao, Jiaqi Liu, Xiangqun Yuan

“…Most protein-coding genes (PCGs) begin with ATN, with 3 types of stop codons including TAA, TAG, and an incomplete codon T-; most of the genes terminate with TAA. …”
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Mitochondrial genome of Chrysochares punctatus (Coleoptera: Chrysomelidae: Eumolpinae) and phylogenetic analysis by Run-Qiu Feng, Li-Jun Zhang, Min Li, Jia Liu, Chun-Li Wen, Ming-Long Yuan

“…Ten PCGs started with a typical ATN codon, whereas the remaining three PCGs started with AAC (cox1) and TTG (nad1 and nad2). …”
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Generation of an induced pluripotent stem cell line (IGGi002A) from nasal cells of a cystic fibrosis patient homozygous for the G542X-CFTR mutation by Michał Dębczyński, Damian Mojsak, Serena Tamburro, Simona Baldassari, Ilaria Musante, Rosaria Casciaro, Fabiana Ciciriello, Federico Zara, Paolo Scudieri, Giulia Gorrieri

“…Several CFTR mutations are causative of CF, among which G542X is a nonsense mutation introducing a premature stop codon which prevents CFTR protein synthesis. We generated a new iPSC line from nasal cells carrying G542X homozygous mutation for CFTR: IGGi002A. …”
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Comparative Mitogenomic Analysis of Two Cuckoo Bees (Apoidea: Anthophila: Megachilidae) with Phylogenetic Implications by Huanhuan Lu, Bo He, Youjin Hao, Zeyang Zhou, Chengyong Su, Dunyuan Huang

“…The obtained results show that 37 mitogenomic genes and one putative control region were conserved within Hymenoptera. Truncated stop codon T was found in the <i>cox3</i> gene of <i>E. polynesia</i>. …”
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Cytotoxic Mechanism of Excess Polyamines Functions through Translational Repression of Specific Proteins Encoded by Polyamine Modulon by Akihiko Sakamoto, Junpei Sahara, Gota Kawai, Kaneyoshi Yamamoto, Akira Ishihama, Takeshi Uemura, Kazuei Igarashi, Keiko Kashiwagi, Yusuke Terui

“…It was suggested that the structure of the mRNA bulged-out region is affected by excess spermidine, so the SD sequence of the <i>rmf</i> mRNA cannot approach initiation codon AUG.…”
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A Case with Laron Syndrome by İlker Tolga ÖZGEN, Esra KUTLU, Yaşar CESUR, Gözde YEŞİL

“…A missense variation leading to a stop codon (W182X) was determined in GH receptor gene. …”
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Design, Expression and Evaluation of Bacteriostatic Activity of Hybrid Antimicrobial Peptide NK-LPd by Ziqi LIU, Zhengyu LIU, Yinhong GUO, Hongfei WANG, Bo YAO

“…The hybrid antimicrobial peptide NK-LPd gene was optimized according to Pichia pastoris codon preference principle. The optimized gene was amplified by gene splicing via overlap extension polymerase chain reaction (gene splicing by overlap extension polymerase chain reaction, SOE-PCR) and inserted into the secretory expression vector pPIC9K. …”
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Identification and Characterization of a New Splicing Variant of Prokineticin 2 by Roberta Lattanzi, Daniela Maftei, Martina Vincenzi, Maria Rosaria Fullone, Rossella Miele

“…In addition, the introduction of the unnatural amino acid p-benzoyl-L-phenylalanine using amber codon suppression technology demonstrated the role of tryptophan at position 212 of PROKR2 for PROK2C binding by photoactivatable cross-linking. …”
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Characteristics of the Patients Aged Less than 40 Years Operated for a Renal Mass by Abdullah Ilktac, Bayram Dogan, Cevper Ersoz, Ganime Coban, Senad Kalkan

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Integrated genomic analyses of acral and mucosal melanomas nominate novel driver genes by Meng Wang, Ishani Banik, A. Hunter Shain, Iwei Yeh, Boris C. Bastian

“…Amplifications of PAK1 and GAB2 were more commonly observed in acral melanomas, whereas SF3B1 R625 codon mutations were unique to mucosal melanomas (12.9%). …”
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Amino Acids 563–566 of the Na⁺/H⁺ Exchanger Isoform 1 C-Terminal Cytosolic Tail Prevent Protein Degradation and Stabilize Protein Expression and Activity by Xiuju Li, Debajyoti Dutta, Martin Jung, Richard Zimmermann, Larry Fliegel

“…A previous study demonstrated that stop codon polymorphisms can result in decreased activity, expression, targeting and enhanced protein degradation. …”
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Molecular epidemiology of potential candidate markers for chloroquine resistance in imported Plasmodium vivax malaria cases in Iran by Sakineh Pirahmadi, Shima Afzali, Akram Abouie Mehrizi, Abbasali Raz, Ahmad Raeisi

“…Results None of the samples had any mutation at codon 976 of pvmdr1, while the 1076 mutation was detected in 96.2% of the examined isolates. …”
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Novel compound heterozygous variants in EMC1 associated with global developmental delay: a lesson from a non-silent synonymous exonic mutation by Ge Wang, Yanli Wang, Chao Gao, Wanqin Xie

“…RT-PCR assay followed by Sanger sequencing reveals that the c.2376G&gt;A variant leads to aberrant splicing, with retention of intron 19 (561bp) in the mature mRNA, which is presumed to introduce a premature translational termination codon (p.(Val792fsTer31)).ConclusionNovel compound heterozygous variants in EMC1 have been identified in individuals with global developmental delay. …”
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Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis by Kadiam C. Venkata Subbaiah, Jiangbin Wu, Wai Hong Wilson Tang, Peng Yao

“…Eukaryotic translation initiation factor 5A (eIF5A), upon being hypusinated, is essential for the translation elongation of proline-codon rich mRNAs. In this study, we found that increased hypusinated eIF5A protein levels were associated with cardiac fibrosis and heart dysfunction in myocardial infarction (MI) mouse models. …”
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Coordination of m⁶A mRNA Methylation and Gene Transcriptome in Sugarcane Response to Drought Stress by Jinju Wei, Haibi Li, Yiyun Gui, Hui Zhou, Ronghua Zhang, Kai Zhu, Xihui Liu

“…In this study, m⁶A sites of sugarcane transcriptome were significantly enriched around the stop codon and within 3′-untranslated regions (3′UTR). …”
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Genome sequence analysis suggests coevolution of the DIS, SD, and Psi hairpins in HIV-1 genomes by Margarita.I. Zarudnaya, Andriy.L. Potyahaylo, Iryna.M. Kolomiets, Leonid.G. Gorb

“…We supposed that the G-quadruplex, located 56 nts downstream of the Gag start codon, may participate in switching of HIV-1 leader RNA from BMH (branched multiple hairpins) to LDI (long distance interaction) conformation.…”
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Korean Red Ginseng increases defective pol gene in peripheral blood mononuclear cells of HIV-1–infected patients; inhibition of its detection during ginseng-based combination thera... by Young Keol Cho, Jung-Eun Kim, Jun-Hee Woo

“…In contrast, the proportion of stop codon appeared not to be affected by KRG treatment. …”
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Genetic Code Expansion System for Tight Control of Gene Expression in <i>Bombyx mori</i> Cell Lines by Wei Lu, Ruolin Wang, Pan Wang, Sanyuan Ma, Qingyou Xia

“…In D[TAG]G, the amber stop codon is recognized as the orthogonal tRNA^Pyl. …”
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Mitochondrial genome variation and intergenomic sequence transfers in Hevea species by Yingfeng Niu, Chengwen Gao, Jin Liu

“…The four mitochondrial genomes had consistent GC contents, codon usages and AT skews. However, there were significant differences in the genome lengths and sequence repeats. …”
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