Development of a recombinant Newcastle disease virus-vectored vaccine for infectious bronchitis virus variant strains circulating in Egypt by Hassanein H. Abozeid, Anandan Paldurai, Berin P. Varghese, Sunil K. Khattar, Manal A. Afifi, Sahar Zouelfakkar, Ayman H. El-Deeb, Magdy F. El-Kady, Siba K. Samal

“…In order to circumvent these issues, and to develop a vaccine that is more relevant to Egypt and its neighboring countries, a recombinant avirulent Newcastle disease virus (rNDV) strain LaSota was constructed to express the codon-optimized S glycoprotein of the Egyptian IBV variant strain IBV/Ck/EG/CU/4/2014 belonging to GI-23 lineage, that is prevalent in Egypt and in the Middle East. …”
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Molecular and Clinical Characterization of <i>CNGA3</i> and <i>CNGB3</i> Genes in Brazilian Patients Affected with Achromatopsia by Rebeca A. S. Amaral, Fabiana L. Motta, Olivia A. Zin, Mariana M. da Palma, Gabriela D. Rodrigues, Juliana M. F. Sallum

“…The majority of <i>CNGA3</i> variants were missense, and the most prevalent <i>CNGB3</i> variant was c.1148delC (p.Thr383Ilefs*13), resulting in a frameshift and premature stop codon, which is compatible with previous publications in the literature. …”
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Detection of PI3K Gene Mutations in Endometrial Cancer in Iran by Masoumeh Elyasi, Manoochehr Tavasoli, Mehri Faghihi, Simin Hemati

“…A novel mutation was found in codon 3059 of exon 20 [C3059T (A1020V)] which has not been reported previously in endometrial cancer.  …”
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DNA DIAGNOSTICS AND MUTATION SPECTRUM OF THE GENE FBN1 IN MARFAN’S SYNDROME by Yu. A. Rogozhina, V. A. Rumyantseva, A. A. Bukaeva, E. V. Zaklyazminskaya

“…In complete analysis of FBN1 gene by fragmentation check of replacements we found 4 premature stop-codons (p.Y181*, p.R516*, p.Q1811*, p.R2776*) and 3 missense variants (C739W, p.C1095S, p. …”
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A dominant negative ADIPOQ mutation in a diabetic family with renal disease, hypoadiponectinemia, and hyperceramidemia by Christopher A. Simeone, Joseph L. Wilkerson, Annelise M. Poss, James A. Banks, Joseph V. Varre, Jose Lazaro Guevara, Edgar Javier Hernandez, Bushra Gorsi, Donald L. Atkinson, Tursun Turapov, Scott G. Frodsham, Julio C. Fierro Morales, Kristina O’Neil, Barry Moore, Mark Yandell, Scott A. Summers, Andrzej S. Krolewski, William L. Holland, Marcus G. Pezzolesi

“…This mutation introduces a frameshift in exon 3, resulting in a premature termination codon that disrupts translation of adiponectin’s globular domain. …”
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Organelle Genomes and Transcriptomes of <i>Nymphaea</i> Reveal the Interplay between Intron Splicing and RNA Editing by Zheng-Shan He, Andan Zhu, Jun-Bo Yang, Weishu Fan, De-Zhu Li

“…</i> ‘Joey Tomocik’, respectively. The target codon preference, the tendency of increasing protein hydrophobicity, and the bias distribution of editing sites are similar in both organelles, suggesting their common evolutionary origin and shared editing machinery. …”
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4346 Potential Sudden Unexpected Death in Epilepsy (SUDEP) Biomarkers in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes with DEPDC5 Loss-of-Function by Yanting Zhao, Helen Zhang, Jack M. Parent, Lori L. Isom

“…RESULTS/ANTICIPATED RESULTS: CRISPR generated heterozygous deletion of 1 base-pair in the first coding exon of DEPDC5 gene, resulting in a premature stop codon, simulated the variants identified in DEPDC5 epilepsy patients. …”
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Three Novel <i>EPCAM</i> Variants Causing Tufting Enteropathy in Three Families by Hasret Ayyıldız Civan, Coleen Leitner, Iris Östreicher, Anna-Maria Schneider, Malte Cremer, Johannes A. Mayr, Rainer Rossi, Thomas Müller, Andreas R. Janecke

“…We identified three novel pathogenic EPCAM variants: a deletion of exon 1 that removes the ATG initiation codon, a missense variant c.326A > G (p.Gln109Arg), and nonsense mutation c.429G > A (p.Trp143*) in a compound heterozygous state with the Mediterranean splice site variant c.556-14A > G (Tyr186Phefs*6). …”
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Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803 by Rui Miao, Hao Xie, Peter Lindblad

“…Moreover, the expression of the ADH encoded by codon-optimized slr1192 and co-expression of IlvC and IlvD were identified as potential approaches to further enhance isobutanol production in Synechocystis PCC 6803. …”
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Germline variation of Ribonuclease H2 genes in ovarian cancer patients by Rahel Polaczek, Peter Schürmann, Lisa-Marie Speith, Robert Geffers, Matthias Dürst, Peter Hillemanns, Tjoung-Won Park-Simon, Clemens Liebrich, Thilo Dörk

“…We identified one patient with a truncating variant in RNASEH2B, p.C44X, resulting in a premature stop codon. This patient had high-grade serous EOC with an 8 years survival after platinum/taxane-based therapy. …”
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GTPBP1 resolves paused ribosomes to maintain neuronal homeostasis by Markus Terrey, Scott I Adamson, Alana L Gibson, Tianda Deng, Ryuta Ishimura, Jeffrey H Chuang, Susan L Ackerman

“…Here, we show that loss of the homologous protein GTPBP1 during tRNA deficiency in the mouse brain also leads to codon-specific ribosome pausing and neurodegeneration, suggesting that these non-redundant GTPases function in the same pathway to mitigate ribosome pausing. …”
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Phylogenetic Analysis of Two New Mitochondrial Genomes of <i>Singapora shinshana</i> and <i>Seriana bacilla</i> from the Karst Region of Southwest China by Ni Zhang, Tianyi Pu, Jinqiu Wang, Weiwen Tan, Zhouwei Yuan, Can Li, Yuehua Song

“…All 13 PCGs started with ATN codons, except for <i>atp8</i> (TTA) and <i>nad5</i> (TTG). …”
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Data on gene cloning, expression, purification, and characterization of the glycoside hydrolase family 11 from Bacillus velezensis by Dinh Minh Tran, To Uyen Huynh, Thi Huyen Nguyen, Tu Oanh Do, Anh Dzung Nguyen

“…The domain structure of the enzyme has a signal peptide and a family 11 catalytic domain. xyA (without peptide sequence) was successfully expressed in E. coli BL21-CodonPlus (DE3)-RIPL cells using the pColdII vector and purified using the HisTrap FF column. …”
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<i>FRMD7</i> Gene Alterations in a Pakistani Family Associated with Congenital Idiopathic Nystagmus by Muhammad Waqar Arshad, Muhammad Imran Shabbir, Saaim Asif, Mohsin Shahzad, Larissa Leydier, Sunil Kumar Rai

“…Leu148 *) gene in affected individuals from the Pakistani family, with CIN resulting in a premature termination codon, further resulting in the formation of a destabilized protein structure that was incomplete. …”
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Comparative Analysis of <i>Luisia</i> (Aeridinae, Orchidaceae) Plastomes Shed Light on Plastomes Evolution and Barcodes Investigation by Liang Ma, Cheng-Yuan Zhou, Jin-Liao Chen, Ding-Kun Liu, Siren Lan, Zhong-Jian Liu

“…Notably, all <i>ndh</i> genes were pseudogenized or lost. An analysis of codon usage bias showed that leucine (Leu) exhibited the highest frequency, while cysteine (Cys) exhibited the lowest frequency. …”
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Comparative Mitogenomics and Phylogenetic Analyses of Pentatomoidea (Hemiptera: Heteroptera) by Shiwen Xu, Yunfei Wu, Yingqi Liu, Ping Zhao, Zhuo Chen, Fan Song, Hu Li, Wanzhi Cai

“…When the family Urostylididae is excluded in taxa sampling or the third codon positions of protein coding genes are removed, phylogenetic analyses under site-homogenous models could provide more stable tree topologies. …”
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Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro system by Fujun Zhou, Julie M Bocetti, Meizhen Hou, Daoming Qin, Alan G Hinnebusch, Jon R Lorsch

“…Our data do not support a model in which Ded1acts by reducing initiation at alternative start codons in 5′UTRs and instead indicate it functions by directly promoting mRNA recruitment to the 43S PIC and scanning to locate the main start codon. …”
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The Association Between Arg72Pro C>G Polymorphism in the p53 Gene and the Risk of Obesity by Gülşah Koç, Ahu Soyocak, Zehra Kaya, Burak Kankaya, Halil Alış

“…Conclusion: Our study is one of the first to investigate the relationship between p53 codon 72 polymorphisms and obesity risk but revealed no correlation between them. …”
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Evolution of Adaptive Variation in the Mosquito <i>Culex pipiens</i>: Multiple Independent Origins of Insecticide Resistance Mutations by Valentina Mastrantonio, Daniele Porretta, Valentina Lucchesi, Nurper Güz, Naciye Sena Çağatay, Romeo Bellini, John Vontas, Sandra Urbanelli

“…Three point mutations confer it at the codon 1043 of the chitin synthase 1 gene (<i>chs-1</i>): I1043L, I1043M, and I1043F. …”
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Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts by Byung-Chun Yoo, Narendra S. Yadav, Emil M. Orozco Jr, Hajime Sakai

“…The plasmids, designated “Edit Plasmids,” were constructed with two expression cassettes, one for the expression of Cas9, codon-optimized for each organelle, under promoters specific to each organelle, and the other cassette for the expression of guide RNAs under another set of promoters specific to each organelle. …”
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