Exploring the origin of a unique mutant allele in twin-tail goldfish using CRISPR/Cas9 mutants by Shu-Hua Lee, Chen-Yi Wang, Ing-Jia Li, Gembu Abe, Kinya G. Ota

“…Here, we performed genetic crossing with twin-tail ornamental goldfish and CRISPR/Cas9-mutated goldfish to investigate why only a single mutated allele—chdS with a E127X stop codon (also called chdA E127X )—gives rise to the twin-tail phenotype in the modern domesticated goldfish population. …”
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Epidemiological, Clinical, and Diagnostic Aspects of Urinary Tract Infection in Newborns at the Departmental Teaching Hospital of Borgou-Alibori (DTH-B/A) in Benin by Séraphin Ahoui, Gerard Kpanidja, Alphonse Noudamadjo, Falilatou Agbeille, Hermione Nelly Djima, Ahmed Adegbola, Aristide Dah, Julien Didier Adedemy, Joseph Agossou

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Hematological and molecular characteristics of a novel α-globin variant Hb Liangqing (HBA2:c.224A>G) by Youqiong Li, Shulin Liang, Liang Liang, Lihong Zheng, Xiaocai Lu

“…However, Sanger sequencing confirmed the presence of a new heterozygous mutation (GAC>GGC) at codon 74 of the HBA2 gene (HBA2:c.224A>G), resulting in a novel Hb variant. …”
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SEQUENCIAMENTO E ANÁLISE DOS GENES DAS SUBUNIDADES ALFA E BETA DO HORMÔNIO FOLÍCULO ESTIMULANTE DE BOVINO (Bos taurus indicus) by Ângela Patrícia Santana, Lidia Pepe Moraes, Luci Sayori Murata, Fernando Araripe Torres

“…Na comparação com ßFSH, registrou-se modificação na primeira base do codon que levou à alteração no resíduo do aminóacido 83, que, em Bos taurus indicus, é uma glicina, ao invés da serina presente em Bos taurus taurus. …”
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Comparative genomic analyses reveal evidence for adaptive A-to-I RNA editing in insect Adar gene by Caiqing Zheng, Ling Ma, Fan Song, Li Tian, Wanzhi Cai, Hu Li, Yuange Duan

“…Recent discoveries on the evolutionary trajectory of Ser>Gly auto-editing site in insect Adar gene demonstrated a selective advantage to having an editable codon compared to uneditable ones. However, apart from pure observations, quantitative approaches for justifying the adaptiveness of individual RNA editing sites are still lacking. …”
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A Novel Method of Affinity Tag Cleavage in the Purification of a Recombinant Thermostable Lipase from Aneurinibacillus thermoaerophilus Strain HZ by Malihe Masomian, Raja Noor Zaliha Raja Abd Rahman, Abu Bakar Salleh

“…N-terminal sequencing of purified recombinant HZ lipase confirmed the elimination of the 17.4 kDa tag from one amino acid before the native start codon (Methionine) of the protein. The mature rHZ lipase was highly active at 65 °C and a pH of 7.0, with a half-life of 2 h 15 min at 55 °C and 45 min at 60 °C. …”
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Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli by Christian Sonnendecker, Ren Wei, Elisabeth Kurze, Jinpeng Wang, Thorsten Oeser, Wolfgang Zimmermann

“…G825-6 was investigated in Escherichia coli BL21(DE3) using a codon-adapted gene. In addition, a novel purification method for the CGTase using starch adsorption was developed. …”
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Exploring Roles of the Polysaccharide Capsule in Pathogenesis of Hypervirulent <i>Acinetobacter baumannii</i> Clinical Isolate Lac-4 by Elisabet Bjånes, Truman Koh, Tariq Qayum, Raymond Zurich, Sinead McCabe, Kegan Hampel, Lisa Cartwright, Victor Nizet

“…We assessed the role of the capsule in wildtype Lac-4 (WT) by generating a premature stop codon in <i>wza</i>, which codes for the polysaccharide export protein. …”
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Assembly and analysis of the complete mitochondrial genome of Forsythia suspensa (Thunb.) Vahl by Yun Song, Xiaorong Du, Aoxuan Li, Amei Fan, Longjiao He, Zhe Sun, Yanbing Niu, Yonggang Qiao

“…We further analyzed RNA editing of the protein-coding genes, relative synonymous codon usage, and sequence repeats based on the genomic data. …”
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APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate i... by Pathak Vinay K, Hu Wei-Shau, Moore Michael D, Russell Rebecca A

“…Additionally, genomes containing start codon mutations and early termination codons within <it>gag </it>were isolated from the vRNA.…”
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The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC by Rongrong Chen, Qianhan Wei, Yan Liu, Jiankun Li, Xuemei Du, Yan Chen, Jianhua Wang, Yunjun Liu

“…A single-nucleotide deletion in the emp601 mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex III as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. …”
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Medial Temporal Lobe Involvement in Human Prion Diseases: Implications for the Study of Focal Non Prion Neurodegenerative Pathology by Alberto Rábano, Carmen Guerrero Márquez, Ramón A. Juste, María V. Geijo, Miguel Calero

“…Most cases (90%) were classified according to PrP^res type and/or <i>PRNP</i> codon 129 status, in addition to a full neuropathological profile. …”
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Identification and functional analysis of m6A in the mammary gland tissues of dairy goats at the early and peak lactation stages by Shujun Wang, Lu Zhang, Rong Xuan, Qing Li, Zhibin Ji, Tianle Chao, Jianmin Wang, Chunlan Zhang

“…The results showed that m6A methylation occurred at 3,927 loci, which were significantly enriched in the 3′ untranslated region (3′UTR) and the termination codon region. In the early stage and peak stage of lactation, m6A methylation occurred extensively in mammary tissues, and a total of 725 differentially expressed m6A-modified genes were obtained, all negatively correlated with mRNA expression. …”
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Analysis of Viral Promoters for Transgene Expression and of the Effect of 5′-UTRs on Alternative Translational Start Sites in <i>Chlamydomonas</i> by Justus Niemeyer, Laura Fischer, Frank O’Neill Aylward, Michael Schroda

“…We show that this problem can be overcome by mutating the responsible methionine codons to codons for leucine and by using the 5′-UTR of <i>βTUB2</i> instead of the 5′-UTRs of <i>PSAD</i> or <i>RBCS2</i>. …”
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The land flatworm Amaga expatria (Geoplanidae) in Guadeloupe and Martinique: new reports and molecular characterization including complete mitogenome by Jean-Lou Justine, Delphine Gey, Jessica Thévenot, Romain Gastineau, Hugh D. Jones

“…The mitogenome is 14,962 bp in length and contains 12 protein coding genes, two rRNA genes and 22 tRNA genes; for two protein genes it was not possible to determine the start codon. The mitogenome was compared with the few available mitogenomes from geoplanids and the most similar was Obama nungara, a species from South America. …”
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Efficient Soluble Expression and Fermentation Conditions of D-Allulose 3-Epimerase in Escherichia coli by Qiufeng LI, Jing CHEN, Jingyi ZHAO, Xin WEI, Zhiqi WANG, Jidong LIU

“…The DAEase gene sequence derived from Clostridium bolteae ATCCBAA-613 was synthesized by codon optimization. Using pCold TF as the expression vector, the cold-shock promoter CspA induced the expression of the DAEase gene in Escherichia coli BL21(DE3) at low temperature. …”
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Central precocious puberty in a boy with X-linked adrenoleukodystrophy caused by a novel ABCD1 mutation by Chaoyue Zhao, Hanhong Zhu, Jie Wang, Wenlong Liu, Yongzhen Xue, Yanyan Hu

“…WES revealed a novel variant (c.1376dup) in the ABCD1 gene, which resulted in a reading frameshift and a premature termination codon (p.Leu461ProfsTer95). Sanger sequencing confirmed that the variant was inherited from his heterozygous mother. …”
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From a variant of unknown significance to pathogenic: Reclassification of a large novel duplication in BRCA2 by high‐throughput sequencing by Jana Lisa vanLuttikhuizen, Janin Bublitz, Stephanie Schubert, Gunnar Schmidt, Winfried Hofmann, Susanne Morlot, Reena Buurman, Bernd Auber, Brigitte Schlegelberger, Doris Steinemann

“…It is predicted to result in a frameshift and a premature stop codon likely triggering nonsense‐mediated mRNA decay. …”
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CRISPR/Cas9‐mediated homology donor repair base editing confers glyphosate resistance to rice (Oryza sativa L.) by Sonia Khan Sony, Tanushri Kaul, Khaled Fathy Abdel Motelb, Arulprakash Thangaraj, Jyotsna Bharti, Rashmi Kaul, Rachana Verma, Mamta Nehra

“…The indigenously designed two-sgRNA OsEPSPS-NICTK-1_pCRISPR-Cas9 construct harboring rice codon-optimized SpCas9 along with OsEPSPS-HDR template was transformed into rice. …”
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A neurodevelopmental disorder caused by a dysfunctional CACNA1A allele by Audra A. Kramer, Daniel F. Bennett, Kristin W. Barañano, Roger A. Bannister

“…The duplicated guanine precipitated 43 residues of altered amino acid sequence beginning with a glutamine to serine substitution in CaV2.1 at position 1674 ending with a premature stop codon (CaV2.1 p.Gln1674Serfs*43). The patient presented with episodic downbeat vertical nystagmus, hypotonia, ataxia, developmental delay and febrile seizures. …”
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