Genetic association analysis of the functional c.714T>G polymorphism and mucosal expression of dectin-1 in inflammatory bowel disease. by Hilbert S de Vries, Theo S Plantinga, J Han van Krieken, Rinke Stienstra, Ad A van Bodegraven, Eleonora A M Festen, Rinse K Weersma, J Bart A Crusius, Ronald K Linskens, Leo A B Joosten, Mihai G Netea, Dirk J de Jong

“…Upon activation, dectin-1 signaling converges, similar to NOD2, on the adaptor molecule CARD9 which is associated with inflammatory bowel disease (IBD). An early stop codon polymorphism (c.714T>G) in DECTIN-1 results in a loss-of-function (p.Y238X) and impaired cytokine responses, including TNF-alpha, interleukin (IL)-1beta and IL-17 upon in vitro stimulation with Candida albicans or beta-glucan. …”
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Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus by Ying-jie Yang, Ye Wang, Zhi-feng Li, Ya Gong, Peng Zhang, Wen-chao Hu, Duo-hong Sheng, Yue-zhong Li

“…We revealed that the high expression of a codon-optimized cas9 gene in M. xanthus was cytotoxic, and developed a temporally high expression strategy to reduce the cell damage from high expressions of Cas9. …”
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An Analysis of Genetic Variability and Population Structure in Wheat Germplasm Using Microsatellite and Gene-Based Markers by Alireza Pour-Aboughadareh, Peter Poczai, Alireza Etminan, Omid Jadidi, Farzad Kianersi, Lia Shooshtari

“…In the current study, the genetic diversity and population structure of 100 individuals from four <i>Triticum</i> and <i>Aegilops</i> species (including <i>T. aestivum</i>, <i>Ae. tauschii</i>, <i>Ae. cylindrica</i>, and <i>Ae. crassa</i>) were investigated using two gene-based markers (start codon targeted (SCoT) polymorphism and CAAT-box derived polymorphism (CBDP)) and simple-sequence repeats (SSRs). …”
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Comparative chloroplast genomes and phylogenetic relationships of Aglaonema modestum and five variegated cultivars of Aglaonema. by Dong-Mei Li, Gen-Fa Zhu, Bo Yu, Dan Huang

“…The molecular structure, gene order, content, codon usage, long repeats, and simple sequence repeats (SSRs) were generally conserved among the six sequenced genomes, but the IR-SSC boundary regions were significantly different, and 'Red Vein' had a distinct long repeat number and type frequency. …”
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Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus by Alea Maurice Simbulan, Edward C. Banico, Ella Mae Joy S. Sira, Nyzar Mabeth O. Odchimar, Fredmoore L. Orosco

“…Moreover, this study designed an optimized codon for efficient translation of the ASFV vaccine construct into the Escherichia coli K-12 expression system using the pET28a(+) vector. …”
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Disorder of Sex Development Due to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 Deficiency: A Case Report and Review of 70 Different <i>HSD17B3</i> Mutations Reported in 239 Patient... by Catarina I. Gonçalves, Josianne Carriço, Margarida Bastos, Manuel C. Lemos

“…Several mutations were recurrent and missense mutations at codon 80 and the splice-site mutation c.277+4A>T each represented 17% of all mutated alleles. …”
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Strategies for Successful Over-Expression of Human Membrane Transport Systems Using Bacterial Hosts: Future Perspectives by Michele Galluccio, Lara Console, Lorena Pochini, Mariafrancesca Scalise, Nicola Giangregorio, Cesare Indiveri

“…Moreover, differences in codon usage between humans and bacteria hamper translation. …”
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Long-term response to pimitespib in postoperative recurrent gastrointestinal stromal tumors with PDGFRA D842V mutation: a case report by Ryugo Teranishi, Tsuyoshi Takahashi, Yukinori Kurokawa, Takuro Saito, Kazuyoshi Yamamoto, Kotaro Yamashita, Koji Tanaka, Tomoki Makino, Kiyokazu Nakajima, Hidetoshi Eguchi, Yuichiro Doki

“…Abstract Background Exon 18 D842V, which is a point mutation from aspartic acid to valine at codon 842, is the most frequent mutation in Platelet-Derived Growth Factor Receptor alpha (PDGFRA)-mutated gastrointestinal stromal tumor (GIST). …”
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Mutations in pseudohypoparathyroidism 1a and pseudopseudohypoparathyroidism in ethnic Chinese. by Yi-Lei Wu, Daw-Yang Hwang, Hui-Pin Hsiao, Wei-Hsin Ting, Chi-Yu Huang, Wen-Yu Tsai, Hung-Chun Chen, Mei-Chyn Chao, Fu-Sung Lo, Jeng-Daw Tsai, Stone Yang, Shin-Lin Shih, Shuan-Pei Lin, Chiung-Ling Lin, Yann-Jinn Lee

“…The latter was the most probable mechanism which caused a frameshift, changing Arg to Ser at residue 280 and invoking a premature termination of translation at codon 300 (R280Sfs*21). Five GNAS mutations in ethnic Chinese with PHP1A and PPHP were reported. …”
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A novel homozygous TUB mutation associated with autosomal recessive retinitis pigmentosa in a consanguineous Chinese family by Wei Xu, Ming Xu, Qinqin Yin, Chuangyi Liu, Qiuxiang Cao, Yun Deng, Sulai Liu, Guiyun He

“…Phylogenetic analysis indicated that the Asn residue at codon 460 of TUB is highly conserved across diverse species from tropicalis to humans. …”
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Exploring Renal Malignancies in Saudi Arabia: Insights from a Tertiary Care Center Study by Ahmed Alasker, Turki Rashed Alnafisah, Mohammad Alghafees, Belal Nedal Sabbah, Areez Shafqat, Abdullah Alhaider, Abdulrahman Alsayyari, Naif Althonayan, Mohammed AlOtaibi, Fisal Tariq Aldokhel, Salman Bin Ofisan, Saud Abdullah Alawad

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Case report: a novel deep intronic splice-altering variant in DMD as a cause of Becker muscular dystrophy by Shala Ghaderi Berntsson, Hans Matsson, Hans Matsson, Anna Kristoffersson, Valter Niemelä, Hermine A. van Duyvenvoorde, Cindy Richel-van Assenbergh, Heleen M. van der Klift, Olivera Casar-Borota, Olivera Casar-Borota, Carina Frykholm, Carina Frykholm, Anne-Marie Landtblom, Anne-Marie Landtblom

“…Eventually, after more than 10 years, an RNA analysis captured abnormal splicing where 154 nucleotides from intron 43 were inserted between exon 43 and 44 resulting in a frameshift and a premature stop codon. Normal splicing of the DMD gene was also observed. …”
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Enhanced Expression of Alcohol Dehydrogenase I in <i>Pichia pastoris</i> Reduces the Content of Acetaldehyde in Wines by Kun Geng, Ying Lin, Xueyun Zheng, Cheng Li, Shuting Chen, He Ling, Jun Yang, Xiangyu Zhu, Shuli Liang

“…In this study, <i>ADH1</i> was successfully expressed in <i>Pichia pastoris</i> GS115 based on codon optimization. Then, the expression level of <i>ADH1</i> was enhanced by replacing its promoter with optimized promoters and increasing the copy number of the expression cassette, with ADH1 being purified using nickel column affinity chromatography. …”
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Complete chloroplast genomes and comparative analysis of Ligustrum species by Lianxiang Long, Yongtan Li, Shijie Wang, Zhenlin Liu, Jinmao Wang, Minsheng Yang

“…Including six other published Ligustrum species, we compared various characteristics such as gene structure, sequence alignment, codon preference, and nucleic acid diversity, and performed positive-selection genes screening and phylogenetic analysis. …”
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The complete mitochondrial genome of Coccotorus beijingensis Lin et Li, 1990 (Coleoptera, Curculionidae, Curculioninae, Anthonomini) and its phylogenetic implications by Sai Jiang, Lina Jiang, Ran Li, Hui Gao, Aijing Zhang, Yurong Yan, Xinzhu Zou, Jixiao Wu, Shuying Xu, Xianfeng Yi, Yujian Li

“…All protein-coding genes initiated with ATN and TTG codons and ended with TAA, TAG or incomplete stop codons (TA, T). …”
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Contribution to the Knowledge of Dicranoptychini (Diptera, Tipuloidea, Limoniidae) in China, with the First Mitochondrial Genome of the Tribe and Its Phylogenetic Implications by Yuanyuan Xu, Shenglin Zhang, Yaru Chen, Guoquan Wang, Ding Yang, Xiao Zhang

“…In addition, the complete mt genome of <i>D. shandongensis</i> sp. nov. is sequenced and annotated, indicating that it is a typical circular DNA molecule with a length of 16,157 bp and shows a similar gene order, nucleotide composition, and codon usage to mt genomes of other Tipuloidea species. …”
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Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma by Xufeng Zhang, Junfu Wang, Yanhua Cao, Kalin Li, Chao Sun, Wen Jiang, Qian Xin, Jue Wang, Tonggang Qi, Shuangde Liu, Yun Luan

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Zearalenone Removal from Corn Oil by an Enzymatic Strategy by Xiaojiao Chang, Hujun Liu, Jing Sun, Jun Wang, Chengcheng Zhao, Wan Zhang, Jie Zhang, Changpo Sun

“…Herein, we targeted enhancing the secretion of ZEN degrading enzyme by <i>Pichia pastoris</i> through constructing an expression plasmid containing three optimized expression cassettes of <i>zlhy-6</i> codon and signal peptides. Further, we explored various parameters of enzymatic detoxification in neutralized oil and analyzed tocopherols and sterols losses in the corn oil. …”
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Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i> by María M. Tavío, Ana S. Ramírez, Carlos Poveda, Rubén S. Rosales, Cristina F. Malla, José B. Poveda

“…Five mutations were found in the L22 protein, one of them at the stop codon. In addition, two mutations were found in the amino acid sequence of L4. …”
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Update on Dihydropteroate Synthase (DHPS) Mutations in <i>Pneumocystis jirovecii</i> by Carmen de la Horra, Vicente Friaza, Rubén Morilla, Juan Delgado, Francisco J. Medrano, Robert F. Miller, Yaxsier de Armas, Enrique J. Calderón

“…The presence of dihydropteroate synthase (DHPS) mutations has been described at codon 55 and 57 and found almost around the world. …”
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