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3321
Intradermal ChAdOx1 Vaccine Following Two CoronaVac Shots: A Case Report
Published 2021-09-01“…The 50% pseudovirus neutralization titer (PVNT50) against lentiviral pseudovirus bearing a codon-optimized spike gene (wild type, alpha, beta, and delta) were 1812.42, 822.99, 1025.42, 1347.13, respectively. …”
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3322
Development and characterization of an enterovirus 71 (EV71) virus-like particles (VLPs) vaccine produced in Pichia pastoris
Published 2020-07-01“…We here report the construction and expression of the P1 and 3C genes of EV71 in Pichia pastoris for producing VLP-based EV71 vaccine antigen with a high yield and simple manufacturing process. Based on codon-optimized P1 and 3C genes, EV71-VLPs were efficiently expressed in Pichia pastoris system, and the expression level reached 270 mg/L. …”
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3323
Calcium/Calmodulin-Dependent Serine Protein Kinase (<i>CASK</i>) Gene Polymorphisms in Pigeons
Published 2023-06-01“…One of the detected polymorphic sites (g.8893G > A), located a very close to the start codon, was selected for genotyping in all individuals. …”
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3324
Genome-wide identification and expression analysis of the EXO70 gene family in grape (Vitis vinifera L)
Published 2021-04-01“…Bioinformatics was used to inquire VvEXO70 genes family members, gene structure, system evolution, cis-acting elements, subcellular and chromosomal localization, collinearity, selective pressure, codon bias and tissue expression. All of VvEXO70s had the conserved pfam03081 domain which maybe necessary for interacting with other proteins. …”
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3325
The Influence of <i>Trctf1</i> Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by <i>Trichoderma reesei</i> with Various Soluble Inducers
Published 2023-08-01“…To evaluate the effect of the <i>Trctf1</i> gene on cellulase synthesis and develop a high-yielding cellulase strain, we established a CRISPR–Cas9 genome editing system in <i>T. reesei</i> Rut C30 using codon-optimized Cas9 protein and in vitro transcribed RNA. …”
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3326
De novo splice site variant of ARID1B associated with pathogenesis of Coffin–Siris syndrome
Published 2019-12-01“…Further, bioinformatic analysis predicted abnormal splicing in a translational frameshift of 11 amino acids and the creation of a premature termination codon. Results found a novel de novo splice site variant c.5025+2T>C in the ARID1B and truncated 1 633 amino acid protein NP_065783.3:p. …”
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3327
Development of a Recombinant Pichinde Virus-Vectored Vaccine against Turkey Arthritis Reovirus and Its Immunological Response Characterization in Vaccinated Animals
Published 2021-02-01“…In addition, three recombinant PICV-based TARV vaccines were produced carrying codon-optimized S1 and/or S3 genes of a TARV strain. …”
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3328
ESTABLISHMENT OF A BACULOVIRUS EXPRESSION SYSTEM FOR PRODUCTION OF THE PORCINE CIRCOVIRUS TYPE 3 (PCV3) VIRUS-LIKE PARTICLES
Published 2023-05-01“…Findings: In this study, we produce the original sequence PCV3 Cap (ori- PCV3Cap) and the codon-optimized sequence of the PCV3 Cap (opti-PCV3Cap), respectively. …”
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3329
Conservation and Diversification of tRNA t<sup>6</sup>A-Modifying Enzymes across the Three Domains of Life
Published 2022-11-01“…The universal <i>N</i><sup>6</sup>-threonylcarbamoyladenosine (t<sup>6</sup>A) modification occurs at position 37 of tRNAs that decipher codons starting with adenosine. Mechanistically, t<sup>6</sup>A stabilizes structural configurations of the anticodon stem loop, promotes anticodon–codon pairing and safeguards the translational fidelity. …”
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3330
Characterization and Analysis of the Mitochondrial Genome of Common Bean (<i>Phaseolus vulgaris</i>) by Comparative Genomic Approaches
Published 2020-05-01“…Among the 31 PCGs, four genes (<i>mttB</i>, <i>nad1</i>, <i>nad4L</i>, and <i>rps10</i>) use ACG as initiation codons, which are altered to standard initiation codons by RNA editing. …”
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3331
The LIKE system, a novel protein expression toolbox for <it>Bacillus subtilis</it> based on the <it>liaI</it> promoter
Published 2012-10-01“…To enhance the performance of the P<sub><it>liaI</it></sub>-derived system, site-directed mutagenesis was employed to optimize the ribosome binding site and alter its spacing to the initiation codon used for the translational fusion. The impact of these genetic modifications on protein production yield was measured using GFP as a model protein. …”
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3332
Study on the genetic diversity of tomato’s cultivars via SCoT marker
Published 2021-12-01“…Objective This study was conducted to investigate the application of the start codon targeted (SCoT) markers in the genetic diversity of tomato. …”
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3333
Renal Fanconi Syndrome Is Caused by a Mistargeting-Based Mitochondriopathy
Published 2016-05-01“…Summary: We recently reported an autosomal dominant form of renal Fanconi syndrome caused by a missense mutation in the third codon of the peroxisomal protein EHHADH. The mutation mistargets EHHADH to mitochondria, thereby impairing mitochondrial energy production and, consequently, reabsorption of electrolytes and low-molecular-weight nutrients in the proximal tubule. …”
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3334
OBTAINING THE RECOMBINANT ORF3 PROTEIN OF HEPATITIS E GENOTYPE 3 AND EVALUATION OF ITS ANTIGENIC PROPERTIES
Published 2018-10-01“…The primary structure of the missing C-terminal region of the ORF3 VGE of the genotype 3 was calculated by bioinformatics methods. Codon optimization of the sequence for biosynthesis in E.coli cells was performed. …”
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3335
Complete chloroplast genome data of Shorea macrophylla (Engkabang): Structural features, comparative and phylogenetic analysis
Published 2023-04-01“…The genome exhibits a similar GC content, gene order, structure, and codon usage when compared to previously reported chloroplast genomes from other plant species. …”
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3336
Plastid Phylogenomic Analyses Reveal a Cryptic Species of <i>Ligusticopsis</i> (Apiaceae, Angiosperms)
Published 2023-04-01“…Moreover, the plastid genomes of 12 <i>Ligusticopsis</i> species, including the new species, were highly conserved in terms of gene order, gene content, codon bias, IR boundaries and SSR content. Overall, the integration of morphological, comparative genomic and phylogenetic evidence indicates that <i>Ligusticopsis litangensis</i> actually represents a new species.…”
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3337
Interleukin gene cloning and expression in E. coli
Published 2021-04-01“…These synthetic genes were synthesised with the E. coli codon usage and inserted in pNZY29 cloning vector (Nzytech). …”
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3338
UPF1 regulates mRNA stability by sensing poorly translated coding sequences
Published 2024-04-01“…We find that the strength of translation initiation, upstream open reading frame (uORF) content, codon optimality, AU-rich elements, microRNA binding sites, and open reading frame (ORF) length function combinatorially to regulate mRNA stability. …”
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3339
Assembly and comparative analysis of the complete multichromosomal mitochondrial genome of Cymbidium ensifolium, an orchid of high economic and ornamental value
Published 2024-04-01“…Repeat sequence analysis and prediction of RNA editing sites revealed a total of 915 dispersed repeats, 162 simple repeats, 45 tandem repeats, and 530 RNA editing sites. Analysis of codon usage showed a preference for codons ending in A/T. …”
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3340
Chloroplast genome analyses of Caragana arborescens and Caragana opulens
Published 2024-02-01“…The two Caragana species exhibited similar codon usage patterns. rpl20-clpP, rps19-rpl2, and rpl23-ycf2 showed the highest nucleotide diversity (pi). …”
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