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3441
Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy
Published 2023-04-01“…The constructed library has a clonal diversity of 10<sup>8</sup> and an amino acid variability that matches the codon distribution set by design at each randomized position. …”
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3442
Variation suggestive of horizontal gene transfer at a lipopolysaccharide (<it>lps</it>) biosynthetic locus in <it>Xanthomonas oryzae </it>pv. <it>oryzae</it>, the bacterial leaf bl...
Published 2004-10-01“…All six open reading frames (ORFs) have atypical G+C content and altered codon usage, which are the hallmarks of genomic islands that are acquired by horizontal gene transfer. …”
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3443
Heterologous expression of pediocin/papA in Bacillus subtilis
Published 2022-05-01“…In this study, two vectors carrying the codon sequence of the mature PapA peptide were constructed, one with and one without a His tag. …”
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3444
Quality of Life of Patients on Peritoneal Dialysis and Contributing Factors
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3445
Immunoinformatics-Aided Design and Evaluation of a Potential Multi-Epitope Vaccine against <i>Klebsiella Pneumoniae</i>
Published 2019-08-01“…<i>In silico</i> reverse translation and codon optimization also confirmed its high expression in <i>E. coli</i> K12 strain. …”
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3446
Understanding Insulin in the Age of Precision Medicine and Big Data: Under-Explored Nature of Genomics
Published 2023-01-01“…Moreover, a rare, poorly characterized African population-enriched variant of INS–IGF2 results in a loss of the stop codon. <i>INS</i> transcript UTR variants rs689 and rs3842753, associated with type 1 diabetes, are found in many pancreatic RNAseq datasets with an elevation of the 3′UTR alternatively spliced <i>INS</i> transcript. …”
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3447
β-Globin Gene Cluster Haplotypes in Iranian Patients with β-Thalassemia
Published 2005-09-01“…IVS I.110 (G→A) mutation was linked to haplotype I. Mutation in codon 30 (G→A) was associated with haplotype V. …”
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3448
Determination of Pathogenicity of Breast Cancer 1 Gene Variants using the American College of Medical Genetics and Genomics and the Association for Molecular Pathology Guidelines
Published 2019-12-01“…Keywords: BRCA1 Gene; Bioinformatics; DNA Sequencing; Nonsense Codon; Splice Donor Site; New Zealand.…”
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3449
Comprehensive genomic analysis of the SARS-CoV-2 Omicron variant BA.2.76 in Jining City, China, 2022
Published 2024-04-01“…A total of 69 amino acid variant sites, 9 amino acid deletions, and 1 stop codon mutation were identified across 18 proteins. …”
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3450
Nonsense-mediated decay machinery in Plasmodium falciparum is inefficient and non-essential
Published 2023-08-01“…ABSTRACT Nonsense-mediated decay (NMD) is a conserved mRNA quality control process that eliminates transcripts bearing a premature termination codon. In addition to its role in removing erroneous transcripts, NMD is involved in post-transcriptional regulation of gene expression via programmed intron retention in metazoans. …”
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3451
Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli
Published 2024-03-01“…To improve the adsorption performance, surface display and codon optimization were carried out. Results In this study, we constructed 24 adsorption engineering strains for Hg2+ and Cr6+, utilizing different strategies. …”
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3452
Impact of β-2 Thr164Ile and combined β-adrenergic receptor polymorphisms on prognosis in a cohort of heart failure outpatients
Published 2010-06-01“…We evaluated the role of the β2-AR Thr164Ile polymorphism at codon 164 on prognosis in a prospective study on 315 adult Brazilian HF patients, predominantly middle-aged Caucasian men in functional class I-II, with severe left ventricular systolic dysfunction. …”
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3453
K65R in subtype C HIV-1 isolates from patients failing on a first-line regimen including d4T or AZT: comparison of Sanger and UDP sequencing data.
Published 2012-01-01“…METHODS: 27 subtype C HIV-1 isolates from treated patients failing on a regimen with d4T or AZT plus lamivudine (3TC) plus nevirapine (NVP) or efavirenz (EFV) and who had been sequenced by Sanger were investigated by UDPS at codon 65 of the reverse transcriptase (RT). 18 isolates from naïve patients and dilutions of a control K65R plasmid were analysed by Sanger plus UDPS. …”
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3454
A Possible Pathogenic PSEN2 Gly56Ser Mutation in a Korean Patient with Early-Onset Alzheimer’s Disease
Published 2022-03-01“…Here, a heterozygous <i>PSEN2</i> point mutation from G to A nucleotide change at position 166 (codon 56; c.166G>A, Gly56Ser) was identified in a 64-year-old Korean female with AD with progressive cognitive memory impairment for the 4 years prior to the hospital visit. …”
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3455
IRF8 Regulates Intrinsic Ferroptosis through Repressing p53 Expression to Maintain Tumor Cell Sensitivity to Cytotoxic T Lymphocytes
Published 2023-01-01“…To restore IRF8 expression in tumor cells, we designed and synthesized codon usage-optimized IRF8-encoding DNA to generate IRF8-encoding plasmid NTC9385R-mIRF8. …”
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3456
Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
Published 2017“…The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. …”
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3457
Improved vectors and genome-wide libraries for CRISPR screening
Published 2017“…To create a new vector capable of producing higher-titer virus (lentiCRISPRv2), we made several modifications, including removal of one of the nuclear localization signals, human-codon optimization of the remaining nuclear localization signal and P2A bicistronic linker sequences, and repositioning of the U6-driven sgRNA cassette (Fig. 1a). …”
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3458
A supervised learning approach for taxonomic classification of core-photosystem-II genes and transcripts in the marine environment
Published 2010“…Results To achieve an accurate and rapid taxonomic classification, we employed a computational approach combining a multi-class Support Vector Machine (SVM) with a codon usage position specific scoring matrix (cuPSSM). …”
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3459
Intragenic and Extragenic Suppressors of Temperature Sensitive Mutations in the Replication Initiation Genes dnaD and dnaB of Bacillus subtilis
Published 2010“…These suppressor mutations caused a change in the anticodon of an alanine tRNA so that it would recognize the mutant codon (threonine) in dnaB and likely insert the wild type amino acid (alanine). …”
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3460
Intragenic and extragenic suppressors of temperature sensitive mutations replication initiation genes dnaD and dnaB of Bacillus subtilis
Published 2010“…These suppressor mutations caused a change in the anticodon of an alanine tRNA so that it would recognize the mutant codon (threonine) in dnaB and likely insert the wild type amino acid (alanine). …”
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