CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus by Ran Peng, Ye Wang, Wan-wan Feng, Xin-jing Yue, Jiang-he Chen, Xiao-zhuang Hu, Zhi-feng Li, Duo-hong Sheng, You-ming Zhang, Yue-zhong Li

“…We firstly optimized the cas9 codon to fit the M. xanthus cells, mutated the gene to inactivate the nuclease activity, and constructed the dCas9-activator system in an epothilone producer. …”
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The complete mitochondrial genome of the North American pale summer sedge caddisfly Limnephilus hyalinus (Insecta: Trichoptera: Limnephilidae) by Harith Al-Baeity, Lauren S. Allard, Leanne Arreza, Theresa A. Asbury, Jahan Aubrey Bandayrel, Sipy Brar, Natassja Ellen Brien, Laura L. Chan, Katherine P. Chimney, Mari-Ann R. de Leon, Alejandra C. Farrell, Brittany A. Forsberg, Himesh Ghimire, Daniel J. Heschuk, Morgan L. Highfield, Darian T. Hole, Gladys M. Ilagan, Amanda Jantz, Moiz S. Kapasi, Oliver E. Ko, Emily V. Krupka, Cassie Lemon, Paulette E. Luna, Courtney L. Marshall, Lucky Princia Mucowinka, Trevor W. Oleniuk, Michaela C. L. Palmer, Ivan Paskvalin, Melissa Rodrigues, Kailee A. Rutherford, Maria Sachs, Renee K. S. Stokowski, Daniel S. Sullivan, Renee R. Taillieu, Matsya R. Thulasiram, Justin H. Tsang, Cobi E. Wiwchar, Jennifer M. Wray, Jeffrey M. Marcus

“…Limnephilus hyalinus COX1 features an atypical CGA start codon while ATP8, NAD1, NAD5, and NAD6 exhibit incomplete stop codons. …”
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Current Genetic Survey and Potential Gene-Targeting Therapeutics for Neuromuscular Diseases by Wei Chiu, Ya-Hsin Hsun, Kao-Jung Chang, Aliaksandr A. Yarmishyn, Yu-Jer Hsiao, Yueh Chien, Chian-Shiu Chien, Chun Ma, Yi-Ping Yang, Ping-Hsing Tsai, Shih-Hwa Chiou, Ting-Yi Lin, Hao-Min Cheng

“…Such manipulations may be directed to any point of the gene expression axis, such as classical gene augmentation, modulating premature termination codon ribosomal bypass, splicing modification of pre-mRNA, etc. …”
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Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice by Zongyi Yi, Yanxia Zhao, Zexuan Yi, Yongjian Zhang, Gangbin Tang, Xiaoxue Zhang, Huixian Tang, Wei Zhang, Ying Zhao, Huayuan Xu, Yuyang Nie, Xueqing Sun, Lijun Xing, Lian Dai, Pengfei Yuan, Wensheng Wei

“…In addition, when AAV-delivered circular arRNAs are systematically administered to a humanized mouse model of Hurler syndrome, it rectifies the premature stop codon precisely and restores the functionality of IDUA enzyme encoded by the Hurler causative gene in multiple organs. …”
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Generation of a human embryonic stem cell reporter line, TMEM119-EGFP, for the visualisation of in vitro differentiated human microglia by Charlotte R. Denman, Hoang-Dai Tran, Min-Kyoung Shin, Jisoo Ryu, Bernd Kuhn, Junghyun Jo

“…Using CRISPR/Cas9 technology, we introduced enhanced green fluorescence protein (EGFP), into the H9 WA-09 human embryonic stem cell line, directly before the TMEM119 stop codon. Sanger sequencing confirmed successful insertion of the EGFP sequence. …”
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pARIS-htt: an optimised expression platform to study huntingtin reveals functional domains required for vesicular trafficking by Pardo Raúl, Molina-Calavita Maria, Poizat Ghislaine, Keryer Guy, Humbert Sandrine, Saudou Frédéric

“…It includes synthetic cDNA coding for full-length human htt modified so that: 1) it is improved for codon usage, 2) it is insensitive to four different siRNAs allowing gene replacement studies, 3) it contains unique restriction sites (URSs) dispersed throughout the entire sequence without modifying the translated amino acid sequence, 4) it contains multiple cloning sites at the N and C-ter ends and 5) it is Gateway compatible. …”
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Amino Acid Substitution within Seven-Octapeptide Repeat Insertions in the Prion Protein Gene Associated with Short-Term Course by Zhongyun Chen, Haitian Nan, Yu Kong, Min Chu, Li Liu, Jing Zhang, Lin Wang, Liyong Wu

“…The clinical presentation of 7-OPRI is significantly heterogeneous. Codon 129 cis-V and amino acid substitution within repeat insertions are possible contributors to the short-term disease course of 7-OPRI.…”
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<i>Verticillium dahliae VdPBP1</i> Transcription Factor Is Required for Hyphal Growth, Virulence, and Microsclerotia Formation by Huong Thi Nguyen, Thanh Thi Duong, Vu Xuan Nguyen, Tien-Dung Nguyen, Thuc Tri Bui, Dung Thuy Nguyen Pham

“…Bioinformatic analysis revealed that the <i>VdPBP1</i> gene consists of four exons and three introns, encoding a 952-codon reading frame. The protein features a 9aaTAD domain, LsmAD, and PAB1 DNA-binding sites, as well as potential nuclear localization and transmembrane helix signals. …”
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<i>S</i>-Methyl-(2-Methoxycarbonylamino-Benzimidazole-5) Thiosulfonate as a Potential Antiparasitic Agent—Its Action on the Development of <i>Ascaris suum</i> Eggs In Vitro by Małgorzata Dmitryjuk, Magdalena Szczotko, Katarzyna Kubiak, Radosław Trojanowicz, Zhanna Parashchyn, Halyna Khomitska, Vira Lubenets

“…Moreover, no SNP was detected at codon 167, which is correlated with benzimidazole resistance, in the tested samples. …”
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Threshold for Pre-existing Antibody Levels Limiting Transduction Efficiency of Systemic rAAV9 Gene Delivery: Relevance for Translation by Aaron S. Meadows, Ricardo J. Pineda, Laurie Goodchild, Tierra A. Bobo, Haiyan Fu

“…No significant differences were observed in vector genome (vg) biodistribution in animals with ≤1:400 total serum αAAV9-IgG compared to αAAV9-Ab-negative animals, following an intravenous (i.v.) rAAV9-hNAGLUop (codon-optimized human α-N-acetylglucosaminidase coding sequence cDNA) injection. …”
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High expression of LipL21 protein of Iranian Leptospira interrogans in E. coli, applicable for diagnostic ELISA by K. Kamalzadeh, M. Esmaelizad, J. Ghalani, P. Khaki, M. Tebianian

“…One dominant LipL21 protein pattern was selected. The codon optimised se-quence was cloned into the pET32a+ expression vector. …”

A gradient of force generation at rest differentiates cardiomyopathy outcomes with variants of actin located at the same residue by Michael R. Jones, Chau Tran, Jaskerat Singh, John F. Dawson

“…To determine how changes in the same codon on the same gene lead to different disease phenotypes, we characterized recombinant R312H- and R312C-ACTC variant proteins. …”
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NAD(P)H:Quinone Oxidoreductase 1 (&lt;em&gt;NQO1&lt;/em&gt;) P187S Polymorphism and Prostate Cancer Risk in Caucasians by Arndt Hartmann, Ferdinand Hofstaedter, Wolf F. Wieland, Sven Wach, Elke Nolte, Christine G. Stoehr, Robert Stoehr

“…The distribution of the &lt;em&gt;NQO1&lt;/em&gt; codon 187 SNP did not differ significantly between prostate cancer patients and the control group (&lt;em&gt;p&lt;/em&gt; = 0.242). …”
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Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene from <i>Trapa bispinosa</i> Roxb. by Shijie Ye, Dongjie Yin, Xiaoyan Sun, Qinyi Chen, Ting Min, Hongxun Wang, Limei Wang

“…According to the specificity of the endogenous expression of foreign genes in <i>Escherichia coli</i>, the adaptation codon of the cloned original genes was optimized for improved expression. …”
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FREQUENCY AND RISK FACTORS OF MULTI-DRUG RESISTANT TUBERCULOSIS IN KHYBER PAKHTUNKHWA, PAKISTAN: A RETROSPECTIVE STUDY by Anita Mughul, Mizna Arif, Furqan Ali Shah, Sikandar Ali Khan, Sommaira Jabeen Shah, Karishma Ali, Shah Fahad Ali Khan, Zia Ullah Shah, Irfan Ullah

“…Results: Out of 1015 cases, 137 (14%) were detected rifampicin-resistant (RR) by Xpert MTB/RIF. Mutation present in codon 531 of the rpoBgene was observed frequently. …”
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The Relationship between Ciprofloxacin Resistance and Genotypic Changes in <i>S. aureus</i> Ocular Isolates by Madeeha Afzal, Ajay Kumar Vijay, Fiona Stapleton, Mark Willcox

“…Of the fourteen resistant isolates, 9/14 had ciprofloxacin resistance mutations within their QRDRs, at codons 80 or 84 within the <i>parC</i> subunit and codon 84 within the <i>gyrA</i> subunit of DNA gyrase. …”
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<it>Mycobacterium tuberculosis </it>spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in South-Western Uganda by Bwanga Freddie, Joloba Moses L, Asiimwe Benon B, Bazira Joel, Matee Mecky I

“…There were three strains with <it>katG </it>(codon 315) gene mutations only while one strain showed the <it>inhA </it>promoter gene mutation.…”
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Antifungal Drug Repurposing by Jong H. Kim, Luisa W. Cheng, Kathleen L. Chan, Christina C. Tam, Noreen Mahoney, Mendel Friedman, Mikhail Martchenko Shilman, Kirkwood M. Land

“…Such long-term application of azole fungicides to crop fields provides environmental selection pressure for the emergence of pan-azole-resistant fungal strains such as <i>Aspergillus fumigatus</i> having TR34/L98H mutations, specifically, a 34 bp insertion into the cytochrome P450 51A (<i>CYP51A</i>) gene promoter region and a leucine-to-histidine substitution at codon 98 of <i>CYP51A</i>. Altogether, the emerging resistance of pathogens to currently available antifungal drugs and insufficiency in the discovery of new therapeutics engender the urgent need for the development of new antifungals and/or alternative therapies for effective control of fungal pathogens. …”
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Mutation of Gemin5 Causes Defective Hematopoietic Stem/Progenitor Cells Proliferation in Zebrafish Embryonic Hematopoiesis by Xiaofen Liu, Wenjuan Zhang, Changbin Jing, Lei Gao, Cong Fu, Chunguang Ren, Yimei Hao, Mengye Cao, Ke Ma, Ke Ma, Weijun Pan, Weijun Pan, Dantong Li, Dantong Li

“…Here, we reported a novel zebrafish mutant line gemin5cas008 with a point mutation in gemin5 gene obtained by ENU mutagenesis and genetic screening, causing an earlier stop codon next to the fifth WD repeat. Gemin5 is an RNA-binding protein with multifunction in post-transcriptional regulation, such as regulating the biogenesis of snRNPs, alternative splicing, stress response, and translation control. …”
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The complete chloroplast genome sequence of Rhododendron fortunei: structural comparative and phylogenetic analysis in the Ericaceae family by Yunli Xiao, Wanjing Zhang, Yirong Sun, Zhiliang Li, Jiaojun Yu, Chunyu Zhang, Shuzhen Wang

“…Leucine (11.51%) and cysteine (1.15%) were the highest and lowest representative amino acids, respectively. The total of 30 codons with obvious codon usage bias were all A/U-ending codons. …”
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