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3601
CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus
Published 2018-01-01“…We firstly optimized the cas9 codon to fit the M. xanthus cells, mutated the gene to inactivate the nuclease activity, and constructed the dCas9-activator system in an epothilone producer. …”
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3602
The complete mitochondrial genome of the North American pale summer sedge caddisfly Limnephilus hyalinus (Insecta: Trichoptera: Limnephilidae)
Published 2019-01-01“…Limnephilus hyalinus COX1 features an atypical CGA start codon while ATP8, NAD1, NAD5, and NAD6 exhibit incomplete stop codons. …”
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3603
Current Genetic Survey and Potential Gene-Targeting Therapeutics for Neuromuscular Diseases
Published 2020-12-01“…Such manipulations may be directed to any point of the gene expression axis, such as classical gene augmentation, modulating premature termination codon ribosomal bypass, splicing modification of pre-mRNA, etc. …”
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3604
Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice
Published 2023-10-01“…In addition, when AAV-delivered circular arRNAs are systematically administered to a humanized mouse model of Hurler syndrome, it rectifies the premature stop codon precisely and restores the functionality of IDUA enzyme encoded by the Hurler causative gene in multiple organs. …”
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3605
Generation of a human embryonic stem cell reporter line, TMEM119-EGFP, for the visualisation of in vitro differentiated human microglia
Published 2023-12-01“…Using CRISPR/Cas9 technology, we introduced enhanced green fluorescence protein (EGFP), into the H9 WA-09 human embryonic stem cell line, directly before the TMEM119 stop codon. Sanger sequencing confirmed successful insertion of the EGFP sequence. …”
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3606
pARIS-htt: an optimised expression platform to study huntingtin reveals functional domains required for vesicular trafficking
Published 2010-06-01“…It includes synthetic cDNA coding for full-length human htt modified so that: 1) it is improved for codon usage, 2) it is insensitive to four different siRNAs allowing gene replacement studies, 3) it contains unique restriction sites (URSs) dispersed throughout the entire sequence without modifying the translated amino acid sequence, 4) it contains multiple cloning sites at the N and C-ter ends and 5) it is Gateway compatible. …”
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3607
Amino Acid Substitution within Seven-Octapeptide Repeat Insertions in the Prion Protein Gene Associated with Short-Term Course
Published 2022-10-01“…The clinical presentation of 7-OPRI is significantly heterogeneous. Codon 129 cis-V and amino acid substitution within repeat insertions are possible contributors to the short-term disease course of 7-OPRI.…”
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3608
<i>Verticillium dahliae VdPBP1</i> Transcription Factor Is Required for Hyphal Growth, Virulence, and Microsclerotia Formation
Published 2024-01-01“…Bioinformatic analysis revealed that the <i>VdPBP1</i> gene consists of four exons and three introns, encoding a 952-codon reading frame. The protein features a 9aaTAD domain, LsmAD, and PAB1 DNA-binding sites, as well as potential nuclear localization and transmembrane helix signals. …”
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3609
<i>S</i>-Methyl-(2-Methoxycarbonylamino-Benzimidazole-5) Thiosulfonate as a Potential Antiparasitic Agent—Its Action on the Development of <i>Ascaris suum</i> Eggs In Vitro
Published 2020-10-01“…Moreover, no SNP was detected at codon 167, which is correlated with benzimidazole resistance, in the tested samples. …”
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3610
Threshold for Pre-existing Antibody Levels Limiting Transduction Efficiency of Systemic rAAV9 Gene Delivery: Relevance for Translation
Published 2019-06-01“…No significant differences were observed in vector genome (vg) biodistribution in animals with ≤1:400 total serum αAAV9-IgG compared to αAAV9-Ab-negative animals, following an intravenous (i.v.) rAAV9-hNAGLUop (codon-optimized human α-N-acetylglucosaminidase coding sequence cDNA) injection. …”
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3611
High expression of LipL21 protein of Iranian Leptospira interrogans in E. coli, applicable for diagnostic ELISA
Published 2023-09-01“…One dominant LipL21 protein pattern was selected. The codon optimised se-quence was cloned into the pET32a+ expression vector. …”
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3612
A gradient of force generation at rest differentiates cardiomyopathy outcomes with variants of actin located at the same residue
Published 2022-12-01“…To determine how changes in the same codon on the same gene lead to different disease phenotypes, we characterized recombinant R312H- and R312C-ACTC variant proteins. …”
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3613
NAD(P)H:Quinone Oxidoreductase 1 (<em>NQO1</em>) P187S Polymorphism and Prostate Cancer Risk in Caucasians
Published 2012-08-01“…The distribution of the <em>NQO1</em> codon 187 SNP did not differ significantly between prostate cancer patients and the control group (<em>p</em> = 0.242). …”
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3614
Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene from <i>Trapa bispinosa</i> Roxb.
Published 2022-11-01“…According to the specificity of the endogenous expression of foreign genes in <i>Escherichia coli</i>, the adaptation codon of the cloned original genes was optimized for improved expression. …”
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3615
FREQUENCY AND RISK FACTORS OF MULTI-DRUG RESISTANT TUBERCULOSIS IN KHYBER PAKHTUNKHWA, PAKISTAN: A RETROSPECTIVE STUDY
Published 2021-12-01“…Results: Out of 1015 cases, 137 (14%) were detected rifampicin-resistant (RR) by Xpert MTB/RIF. Mutation present in codon 531 of the rpoBgene was observed frequently. …”
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3616
The Relationship between Ciprofloxacin Resistance and Genotypic Changes in <i>S. aureus</i> Ocular Isolates
Published 2022-11-01“…Of the fourteen resistant isolates, 9/14 had ciprofloxacin resistance mutations within their QRDRs, at codons 80 or 84 within the <i>parC</i> subunit and codon 84 within the <i>gyrA</i> subunit of DNA gyrase. …”
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3617
<it>Mycobacterium tuberculosis </it>spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in South-Western Uganda
Published 2011-03-01“…There were three strains with <it>katG </it>(codon 315) gene mutations only while one strain showed the <it>inhA </it>promoter gene mutation.…”
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3618
Antifungal Drug Repurposing
Published 2020-11-01“…Such long-term application of azole fungicides to crop fields provides environmental selection pressure for the emergence of pan-azole-resistant fungal strains such as <i>Aspergillus fumigatus</i> having TR34/L98H mutations, specifically, a 34 bp insertion into the cytochrome P450 51A (<i>CYP51A</i>) gene promoter region and a leucine-to-histidine substitution at codon 98 of <i>CYP51A</i>. Altogether, the emerging resistance of pathogens to currently available antifungal drugs and insufficiency in the discovery of new therapeutics engender the urgent need for the development of new antifungals and/or alternative therapies for effective control of fungal pathogens. …”
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3619
Mutation of Gemin5 Causes Defective Hematopoietic Stem/Progenitor Cells Proliferation in Zebrafish Embryonic Hematopoiesis
Published 2021-04-01“…Here, we reported a novel zebrafish mutant line gemin5cas008 with a point mutation in gemin5 gene obtained by ENU mutagenesis and genetic screening, causing an earlier stop codon next to the fifth WD repeat. Gemin5 is an RNA-binding protein with multifunction in post-transcriptional regulation, such as regulating the biogenesis of snRNPs, alternative splicing, stress response, and translation control. …”
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3620
The complete chloroplast genome sequence of Rhododendron fortunei: structural comparative and phylogenetic analysis in the Ericaceae family
Published 2023-10-01“…Leucine (11.51%) and cysteine (1.15%) were the highest and lowest representative amino acids, respectively. The total of 30 codons with obvious codon usage bias were all A/U-ending codons. …”
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