-
3701
The Mitogenome of <i>Sedum plumbizincicola</i> (Crassulaceae): Insights into RNA Editing, Lateral Gene Transfer, and Phylogenetic Implications
Published 2022-11-01“…We further identified totally 508 RNA editing sites in PCGs, and demonstrated that the second codon positions of mitochondrial genes are most prone to RNA editing events. …”
Get full text
Article -
3702
Comparative mitogenomic analysis of mirid bugs (Hemiptera: Miridae) and evaluation of potential DNA barcoding markers
Published 2017-08-01“…Our results showed that the general mitogenomic features (gene content, gene arrangement, base composition and codon usage) were well conserved among these mirids. …”
Get full text
Article -
3703
Effects of Efavirenz for the Treatment of HIV on the Basis of Genetic Polymorphisms
Published 2022-04-01“…One such polymorphism, rs3745274, where there is a change from G to T at codon 516 results in an amino acid change from glutamine to histidine at position 172. …”
Get full text
Article -
3704
The population structure and genetic diversity of Listeria monocytogenes ST9 strains based on genomic analysis
Published 2022-11-01“…The ST9 isolates were divided into three clusters after phylogenetic analysis; 67.63% of ST9 isolates contained putative plasmids with different sizes and genomic structure, the putative prophages inserted in the chromosome at ten hotspots, and seven types of premature stop codon (PMSC) mutations in inlA were found in 81.86% of the ST9 isolates. …”
Get full text
Article -
3705
Case Report: Identification of a rare nonsense mutation in the POC1A gene by NGS in a diabetes mellitus patient
Published 2023-03-01“…Genetic screening revealed that there was a pathogenic variant at the POC1A gene locus and a cytosine-to-thymine mutation at the G81 locus, turning the Arg to a termination codon and shortening the POC1A protein from 359 amino acids (aa) to 80 aa. …”
Get full text
Article -
3706
Five new mitogenomes sequences of Calidridine sandpipers (Aves: Charadriiformes) and comparative mitogenomics of genus Calidris
Published 2022-04-01“…There were four start codons, four stop codons, and one incomplete stop codon (T–). …”
Get full text
Article -
3707
NGS-Based Analysis of Atypical Deep Penetrating Nevi
Published 2021-06-01“…The only case with progressive nodal disease carried alterations in the β-catenin pathway and mutations in IDH1 and NRAS (codon 61). By a comprehensive mutation analysis, we found low genetic heterogeneity and a lack of significant associations between specific gene mutations and histopathological features, despite atypical features. …”
Get full text
Article -
3708
Immunoinformatics-aided design of a new multi-epitope vaccine adjuvanted with domain 4 of pneumolysin against Streptococcus pneumoniae strains
Published 2023-02-01“…The structural stability of the docked complex was confirmed by molecular dynamics. Finally, codon optimization was performed for gene expression in E. coli, followed by in silico cloning in the pET28a(+) vector. …”
Get full text
Article -
3709
Two Simple Methods for Optimizing the Production of "Difficult-to-Express" GnRH-DFF40 Chimeric Protein
Published 2019-08-01“…Here we introduce two successful and reproducible conditions for production and purification of "difficult-to-express" GnRH-DFF40 protein. Methods: A synthetic codon optimized GnRH-DFF40 fusion gene was cloned in pET28a plasmid. …”
Get full text
Article -
3710
Precision Medicine in Systemic Mastocytosis
Published 2021-10-01“…More than 80% of patients with SM carry a somatic point mutation of <i>KIT</i> at codon 816, which may be targeted by kinase inhibitors. …”
Get full text
Article -
3711
Comparative and Phylogenetic Analysis of Complete Plastomes among Aristidoideae Species (Poaceae)
Published 2022-01-01“…The number of SSRs ranged from 30 (<i>Sartidia isaloensis</i>) to 54 (<i>Aristida purpurea</i>). Codon usage analysis showed that plastome genes preferred to use codons ending with A/T. …”
Get full text
Article -
3712
Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology
Published 2023-04-01“…Objective·To establish a T-box transcription factor Brachyury (T gene) fluorescence reporter cell line, in which foot-and-mouth disease virus 2A (F2A) and enhanced green fluorescent protein (EGFP) were knocked in at the end of mouse T gene (mT-F2A-EGFP) in mouse embryonic stem cells (mESCs) by CRISPR/Cas9n (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 nickase)-mediated homologous-directed repair (HDR) technology.Methods·First of all, the specific single guide RNA (sgRNA) plasmid targeting the sequence near the stop codon of the mouse T gene and the plasmid donor containing F2A-EGFP were constructed. …”
Get full text
Article -
3713
Role of BgaA as a Pneumococcal Virulence Factor Elucidated by Molecular Evolutionary Analysis
Published 2020-09-01“…Our evolutionary analysis showed that among the examined genes, nanA and bgaA had high proportions of codon that were under significant negative selection. …”
Get full text
Article -
3714
Comparative mitochondrial genome analysis of three leafhopper species of the genus Abrus Dai & Zhang (Hemiptera: Cicadellidae: Deltocephalinae) from China with phylogenetic implica...
Published 2023-11-01“…The nucleotide composition of these genomes is highly biased toward A + T nucleotides (76.2%, 76.3%, and 74.7%), AT-skews (0.091 to 0.095, and 0.095), negative GC-skews (− 0.138, − 0.161, and − 0.138), and codon usage. All 22 tRNA genes had typical cloverleaf secondary structures, except for trnS1 (AGN) which lacks the dihydrouridine arm, and distinctively trnG in the mitogenome of A. expansivus lacks the TψC arm. …”
Get full text
Article -
3715
Transcriptome-wide N6-methyladenosine methylation profile of atherosclerosis in mice
Published 2023-12-01“…Peaks mainly appeared in the coding sequence (CDS) regions close to the stop codon with the RRACH motif. Moreover, functional enrichment analysis demonstrated that m6A-containing genes were significantly enriched in AS-relevant pathways. …”
Get full text
Article -
3716
A Novel Pathogenic IGSF1 Variant in a Patient with GH and TSH Deficiency Diagnosed by High IGF-I Values at Transition to Adult Care
Published 2023-12-01“…This prompted an analysis of IGSF1, in which a novel hemizygous variant causing a stop codon at c.3559C>T (p.Q1187*) was found, confirming the diagnosis of IGSF1 deficiency syndrome. …”
Get full text
Article -
3717
-
3718
Comparative Activity of Six Recombinant Stilbene Synthases in Yeast for Resveratrol Production
Published 2020-07-01“…<i>atropurpurea</i>, <i>Rheum tataricum</i>, <i>Vitis vinifera</i> and <i>Arachis hypogaea</i>. Codon optimized versions of these sequences were inserted in an expression vector flanked by the constitutive PGK and GPD promoters before expression in <i>Saccharomyces cerevisiae</i>. …”
Get full text
Article -
3719
Characterization of the morphology and complete mitochondrial genomes of Eupteryx minusula and Eupteryx gracilirama (Hemiptera: Cicadellidae: Typhlocybinae) from Karst area, Southw...
Published 2021-11-01“…All of the protein-coding genes are starting with ATN, except for some in mitogenome, which has a single T or TAN as a stop codon. All tRNAs have the typical cloverleaf-shaped structure except for trnS1 (AGN) (E. minuscula) which has a reduced DHU arm. …”
Get full text
Article -
3720
Complete Chloroplast Genome Sequences of Four Species in the <i>Caladium</i> Genus: Comparative and Phylogenetic Analyses
Published 2022-11-01“…The effective number of codons (ENC) values and neutrality plot analyses provided the foundation that the natural selection pressure could greatly affect the codon preference. …”
Get full text
Article