Construction and immunogenicity of an mRNA vaccine against chikungunya virus by Jingjing Liu, Jingjing Liu, Xishan Lu, Xingxing Li, Weijin Huang, Enyue Fang, Wenjuan Li, Xiaohui Liu, Minglei Liu, Jia Li, Ming Li, Zelun Zhang, Haifeng Song, Bo Ying, Yuhua Li, Yuhua Li

“…Moreover, compared with the wild-type vaccine, the codon-optimized vaccine elicited robust CD8+ T-cell responses and mild neutralizing antibody titers. …”
Get full text

Novel frameshift mutation in LIS1 gene is a probable cause of lissencephaly: a case report by Camila Simoes, Martín Graña, Soledad Rodriguez, Federico Baltar Yanes, Alejandra Tapié, Nicolás Dell’Oca, Hugo Naya, Víctor Raggio, Lucía Spangenberg

“…The insertion causes a change in the reading frame, and produces a premature stop codon, severely affecting the protein function and probably the silencing of one allele. …”
Get full text

Synthesis and assembly of full-length cyanophage A-4L genome by Ting Zhang, Bonan Xu, Jia Feng, Pingbo Ge, Guorui Li, Jiabao Zhang, Jianting Zhou, Jianlan Jiang

“…We propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ORFs. …”
Get full text

Conformational determinants necessary for secretion of Paecilomyces thermophila β-1,4-xylosidase that lacks a signal peptide by Yalin Yang, Juan Li, Qiang Yu, Junxiu Hou, Chenchen Gao, Dong Li, Yang Liu, Chao Ran, Zhigang Zhou

“…These mutants were expressed as recombinant, codon-optimized proteins in E. coli. Notably, only mutants containing blades 2–4 were effectively secreted. …”
Get full text

Inter- and Intra-specific Genetic Diversity in Diospyros Using SCoT and IRAP Markers by Changfei Guan, Sadaruddin Chachar, Pingxian Zhang, Chaoqiong Hu, Renzi Wang, Yong Yang

“…In this study, we investigated the genetic diversity and relationship among 268 Diospyros accessions from different regions in China using start codon-targeted (SCoT) and inter-retrotransposon-amplified polymorphism (IRAP) markers. …”
Get full text

The complete mitochondrial genome of Aglaia odorata, insights into its genomic structure and RNA editing sites by Zhigang Hao, Zhigang Hao, Zhigang Hao, Zhiping Zhang, Jinan Zhang, Xiufen Cui, Xiufen Cui, Jianqiang Li, Jianqiang Li, Laixin Luo, Laixin Luo, Yingbin Li

“…Additionally, we analyzed codon usage and mitochondrial plastid DNAs (MTPTs). …”
Get full text

Stress Response in Entamoeba histolytica Is Associated with Robust Processing of tRNA to tRNA Halves by Manu Sharma, Hanbang Zhang, Gretchen Ehrenkaufer, Upinder Singh

“…In particular, tRNA halves, a class of tRNA fragments produced by the cleavage of tRNAs in the anti-codon loop, have been widely reported to accumulate under stress and regulate translation in cells. …”
Get full text

A computational approach to identify point mutations associated with occult hepatitis B: significant mutations affect coding regions but not regulative elements of HBV by Presti Alessandra, Tritarelli Elena, Villano Umbertina, Amadori Alessandra, Marcantonio Cinzia, Prosperi Mattia, Bruni Roberto, Ciccozzi Massimo, Ciccaglione Anna R

“…All viral proteins, with the only exception of the X, were affected. Stop codons in the S, that might account for absence of serum HBsAg, were not significantly enriched in OBI sequences. …”
Get full text

The Alteration of m6A Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester by Jiajie She, Jiajie She, Kaifen Tan, Jie Liu, Shuo Cao, Zengguang Li, You Peng, Zhuoyu Xiao, Ruiying Diao, Liping Wang

“…These m6A peaks were mainly located in the coding region near the stop codon and were mainly characterized by AUGGAC and UGGACG motif. …”
Get full text

Computational approach for identifying immunogenic epitopes and optimizing peptide vaccine through in-silico cloning against Mycoplasma genitalium by Asma Akter, Natasha Farhin Ananna, Hedayet Ullah, Sirajul Islam, Md. Al Amin, K. M. Kaderi Kibria, Shahin Mahmud

“…Our immune simulation analysis demonstrates immune memory development and robust immunity, while codon adaptation suggests optimal expression in E. coli using the pET-28a(+) vector. …”
Get full text

Complete Chloroplast Genome Sequence of Endangered Species in the Genus <i>Opisthopappus</i> C. Shih: Characterization, Species Identification, and Phylogenetic Relationships by Xinke Zhang, Guoshuai Zhang, Yuan Jiang, Linfang Huang

“…The repeat sequences, codon usage, RNA-editing sites, and comparative analyses revealed a high degree of conservation between the two species. …”
Get full text

A novel viral vaccine platform based on engineered transfer RNA by Tong-Yun Wang, Fan-Dan Meng, Guo-Ju Sang, Hong-Liang Zhang, Zhi-Jun Tian, Hao Zheng, Xue-Hui Cai, Yan-Dong Tang

“…Controlling the replication of premature termination codon (PTC)-containing viruses is a promising approach to generate live but replication-defective viruses that can be used for potent vaccines. …”
Get full text

Characteristics of plastid genomes in the genus Ceratostigma inhabiting arid habitats in China and their phylogenomic implications by Yu-Juan Zhao, Jian Liu, Gen-Shen Yin, Xun Gong

“…All plastomes are highly conservative and similar in gene order, simple sequence repeats (SSRs), long repeat repeats and codon usage patterns, but some structural variations in the border of single copy and inverted repeats. …”
Get full text

Genome-wide transposon mutagenesis analysis of Burkholderia pseudomallei reveals essential genes for in vitro and in vivo survival by Yee-Chin Wong, Raeece Naeem, Moataz Abd El Ghany, Moataz Abd El Ghany, Moataz Abd El Ghany, Moataz Abd El Ghany, Chee-Choong Hoh, Arnab Pain, Sheila Nathan

Get full text

Characterization and Comparative Analysis of the Complete Plastomes of Five <i>Epidendrum</i> (Epidendreae, Orchidaceae) Species by Zhuang Zhao, Meng-Yao Zeng, Yu-Wei Wu, Jin-Wei Li, Zhuang Zhou, Zhong-Jian Liu, Ming-He Li

“…Analysis of relative synonymous codon usage (RSCU) indicated that leucine (Leu) was the most and cysteine (Cys) was the least. …”
Get full text

5′-UTR allelic variants and expression of the lycopene-ɛ-cyclase <i>LCYE</i> gene in maize (<i>Zea mays</i> L.) inbred lines of Russian selection by D. Kh. Arkhestova, B. R. Shomakhov, A. V. Shchennikova, E. Z. Kochieva

“…Compared to A2, allele A5 contained two deletions (at positions -267–260 and -296–290 from the ATG codon) and a G251→T substitution, while allele A6 contained one deletion (-290–296) and two SNPs (G251→T, G265→T). …”
Get full text

Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c by Giuseppe D’Auria, Yordan Hodzhev, Michel de Jesús Aceves-Sánchez, Andrés Moya, Stefan Panaiotov, Mario Alberto Flores-Valdez

“…Results: The complete catalog of variants in genes relative to the reference genome BCG Pasteur 1173P2 (GenBank NC008769) showed that the parental strain BCG Pasteur ATCC 35734, from which the mutant BCGΔBCG1419c originated, showed five synonymous mutations, three missense mutations, and five codon insertions, whereas the BCGΔBCG1419c mutant reported the same changes. …”
Get full text

<em>PSEN1</em> Compound Heterozygous Mutations Associated with Cerebral Amyloid Angiopathy and Cognitive Decline Phenotype by Ilaria Palmieri, Marialuisa Valente, Lisa Maria Farina, Simone Gana, Brigida Minafra, Roberta Zangaglia, Orietta Pansarasa, Daisy Sproviero, Alfredo Costa, Claudio Pacchetti, Anna Pichiecchio, Stella Gagliardi, Cristina Cereda

“…However, mutations after codon 200 in the presenilin 1 (<i>PSEN1</i>) gene have been reported to facilitate CAA onset. …”
Get full text

Identification of novel branch points reveals insights into RNA processing by Gould, Genevieve Michelle

Get full text

An extensive allelic series of Drosophila kae1 mutants reveals diverse and tissue-specific requirements for t6A biogenesis by Lin, Ching-Jung, Smibert, Peter, Zhao, Xiaoyu, Ramroop, Johnny, Benton, Matthew A., Govind, Shubha, Sternglanz, Rolf, Lai, Eric C., Hu, Jennifer F., Kellner, Stefanie M, Dedon, Peter C

“…This modification occurs at high frequency at position 37 of most tRNAs that decode ANN codons, and stabilizes cognate anticodon–codon interactions. …”
Get full text
Get full text
Get full text