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3921
Anti-malarial resistance in Mozambique: Absence of Plasmodium falciparum Kelch 13 (K13) propeller domain polymorphisms associated with resistance to artemisinins
Published 2023-05-01“…Most (56.3%) of the reported non-synonymous mutations were due to substitution at the first base of the codon, 25% at the second base and 18.8% at the third base. …”
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3922
Gene Expression and Characterization of Iturin A Lipopeptide Biosurfactant from <i>Bacillus aryabhattai</i> for Enhanced Oil Recovery
Published 2022-06-01“…An iturin A gene sequence was codon-optimized, amplified with gene-specific primers, and ligated into the pET-32A expression vector to achieve high-level protein expression. …”
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3923
Overexpression of LT, an Oncoprotein Derived from the Polyomavirus SV40, Promotes Somatic Embryogenesis in Cotton
Published 2022-05-01“…In this study, <i>LT</i> gene (SV40 large T antigen), which promotes the transformation efficiency of animal cells, was codon-optimized. Its overexpression vector was transformed into cotton. …”
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3924
Papillary Renal Cell Carcinoma: Demographics, Survival Analysis, Racial Disparities, and Genomic Landscape
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3925
Optimization and Recombinant Expression of Antimicrobial Peptide Molluscidin in Pichia pastoris and Its Antibacterial Activity
Published 2022-02-01“…Methods: The optimized nucleotide sequence of Molluscidin were synthesized according to P. pastoris codon usage frequency. The physicochemical properties were analyzed by bioinformatics. …”
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3926
Identification of Salmonella Pullorum Factors Affecting Immune Reaction in Macrophages from the Avian Host
Published 2023-06-01“…Mutants in genes with conserved single nucleotide polymorphisms (SNPs) between the two serovars in the region 100 bp upstream of the start codon (conserved upstream SNPs [CuSNPs]) such as sseE, osmB, tolQ, a putative immune antigen, and a putative persistent infection factor, exhibited differences in induction of inflammatory cytokines compared to wild-type SP, suggesting a possible role of these CuSNPs in immune regulation. …”
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3927
Diversity and Resistance to Thiophanate-Methyl of <i>Colletotrichum</i> spp. in Strawberry Nursery and the Development of Rapid Detection Using LAMP Method
Published 2022-11-01“…Based on the point mutation at codon 198 (GAG→GCG) in the <i>β-tubulin</i> gene of <i>Colletotrichum</i> isolates, we developed a loop-mediated isothermal amplification (LAMP) assay to rapidly detect the E198A mutants. …”
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3928
Triple Reporter Assay: A Non-Overlapping Luciferase Assay for the Measurement of Complex Macromolecular Regulation in Cancer Cells Using a New Mushroom Luciferase–Luciferin Pair
Published 2023-08-01“…</i> In vitro and in vivo assessments showed that human-codon-optimized <i>Luz</i> (<i>hLuz</i>) has significantly higher activity than native <i>Luz</i> in various cancer cell types. …”
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3929
VEXAS and Myelodysplastic Syndrome: An Interdisciplinary Challenge
Published 2024-02-01“…Somatic mutation in the <i>UBA1</i> codon 41 were found in all patients by next-generation sequencing. …”
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3930
Analysis of Genetic Diversity and Phylogenetic Relationships of Wheat (<i>Triticum aestivum</i> L.) Genotypes Using Phenological, Molecular and DNA Barcoding Markers
Published 2022-12-01“…The present study evaluated 16 wheat genotypes using 19 morphological and phenological traits, 16 molecular markers (Inter Simple Sequence Repeats and Start Codon Targeted; ISSR and SCoT) and <i>rbcL</i> and <i>matK</i> plastid gene barcoding. …”
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3931
Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model
Published 2024-01-01“…The model suggests that the length of poly(A) is negatively correlated with protein production, whereas poly(A) located between 10 and 30 nt upstream of the start codon (AUG) exhibits a weak positive effect on protein abundance. …”
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3932
Comparative Analysis of Chloroplast Genomes within <i>Saxifraga</i> (Saxifragaceae) Takes Insights into Their Genomic Evolution and Adaption to the High-Elevation Environment
Published 2022-09-01“…The genome size, structure, gene content, GC content, and codon usage pattern were found to be highly similar. …”
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3933
Complete chloroplast genomes provide insights into evolution and phylogeny of Zingiber (Zingiberaceae)
Published 2023-01-01“…The genome structures, gene contents, amino acid frequencies, codon usage patterns, RNA editing sites, simple sequence repeats and long repeats are conservative in the genomes of Zingiber. …”
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3934
Interspecies hybridization between Auricularia cornea cv. Yu Muer and Auricularia heimuer cv. Bai Muer through protoplast fusion
Published 2023-10-01“…Inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) profile analysis of fusants and parents depicted that fusants contained polymorphic bands, which indicated the rearrangement and deletion of deoxyribonucleic acid (DNA) in the fusants. …”
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3935
Probing the Conformational States of Thimet Oligopeptidase in Solution
Published 2022-06-01“…<i>p</i>NF was biosynthetically incorporated with high efficiency using the amber codon suppression technology. Inhibitor binding induced shorter Donor-Acceptor (D-A) distances in all mutants, supporting the view that a hinge-like movement is operative in TOP. …”
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3936
Infrageneric Plastid Genomes of <i>Cotoneaster</i> (Rosaceae): Implications for the Plastome Evolution and Origin of <i>C. wilsonii</i> on Ulleung Island
Published 2022-04-01“…The plastomes were highly conserved, with sizes ranging from 159,595 bp (<i>C. tenuipes</i>) to 160,016 bp (<i>C. hebephyllus</i>), and had a GC content of 36.6%. The frequency of codon usage showed similar patterns among the 15 <i>Cotoneaster</i> species, and 24 of the 35 protein-coding genes were predicted to undergo RNA editing. …”
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3937
Characterization of the Complete Mitochondrial Genome of Eight Diurnal Hawkmoths (Lepidoptera: Sphingidae): New Insights into the Origin and Evolution of Diurnalism in Sphingids
Published 2022-09-01“…The most commonly used codons were UUA (Leu), AUU (Ile), UUU (Phe), AUA (Met), and AAU (Asn), whereas GCG (Ala) and CCG (Pro) were rarely used. …”
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3938
The brain-derived neurotrophic factor Val66Met polymorphism increases segregation of structural correlation networks in healthy adult brains
Published 2020-08-01“…Since a valine-to-methionine substitution at codon 66 of the BDNF gene (BDNF Val66Met single nucleotide polymorphism (SNP)) is well-known to have effects on brain structure and function, we hypothesized that SCNs are affected by the BDNF Val66Met SNP. …”
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3939
Genetic Diversity and Selective Pressure in Hepatitis C Virus Genotypes 1–6: Significance for Direct-Acting Antiviral Treatment and Drug Resistance
Published 2015-09-01“…Despite its large genetic diversity, across all genotypes, codon positions were rarely identified to be positively selected (0.23%–0.46%) and predominantly found to be under negative selective pressure, suggesting mainly neutral evolution. …”
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3940
Activated Natural Killer Cell Inoculation Alleviates Fibrotic Liver Pathology in a Carbon Tetrachloride-Induced Liver Cirrhosis Mouse Model
Published 2023-04-01“…For in vivo imaging, NK cells were isolated from codon-optimized luciferase-expressing transgenic mice. …”
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