A versatile genetic-encoded reporter for magnetic resonance imaging by Haiyang Yang, Zhengjiang Qian, Chunhua Liu, Changjun Tie, Aoling Cai, Jie Wang, Yao Xing, Jun Xia, Xiang Li

“…The GEM reporter was developed through codon modification of a bacteria-originated manganese (Mn) binding protein, allowing the sequestration of endogenous Mn in local tissues. …”
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Prediction of RNA editing sites and genome-wide characterization of PERK gene family in maize (Zea mays L.) in response to drought stress by Shahmeer Shahid, Muhammad Ali Sher, Furqan Ahmad, Shoaib ur Rehman, Babar Farid, Humayun Raza, Zulfiqar Ali, Amir Maqbool, Saleh Alfarraj, Mohammad Javed Ansari

“…These transitions were mostly based on changes in the first and second codon bases. The in-silico expression analysis of transcriptome data revealed the differential expression of ZmPERK genes in response to drought stress and oil content accumulation. …”
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On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes by Daniela Pinto, Raquel Gonçalo, Mariana Louro, Marta Sousa Silva, Guillem Hernandez, Tiago N. Cordeiro, Carlos Cordeiro, Carlos São-José

“…While less frequently reported, specific cases exist in which two overlapping ORFs are in frame and share the stop codon. Here, we studied the occurrence of this genetic arrangement in endolysins, the phage enzymes that cut the bacterial cell wall peptidoglycan to release the virion progeny. …”
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Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes by Manuel F Juette, Jordan D Carelli, Emily J Rundlet, Alan Brown, Sichen Shao, Angelica Ferguson, Michael R Wasserman, Mikael Holm, Jack Taunton, Scott C Blanchard

“…Rapid and accurate mRNA translation requires efficient codon-dependent delivery of the correct aminoacyl-tRNA (aa-tRNA) to the ribosomal A site. …”
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Highly rearranged gene orders and rapid evolutionary rates in the mitochondrial genomes of Apodida (Echinodermata: Holothuroidea) by Shao’e Sun, Shao’e Sun, Shao’e Sun, Shao’e Sun, Gang Ni, Wansheng Wang, Zhongli Sha, Zhongli Sha, Zhongli Sha, Zhongli Sha, Ning Xiao, Ning Xiao, Ning Xiao, Ning Xiao, Zeng Xiaoqi

“…Phylogenetic relationships based on two different criteria: (i) nucleotides sequences with all codon positions (PCG123) and (ii) Neutral Transitions Excluded model (NTE) confirmed the monophyly and basal position of the order Apodida with a long branch. …”
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NPC1 silent variant induces skipping of exon 11 (p.V562V) and unfolded protein response was found in a specific Niemann‐Pick type C patient by Marisa Encarnação, Maria Francisca Coutinho, Soo Min Cho, Maria Teresa Cardoso, Isaura Ribeiro, Paulo Chaves, Juliana Inês Santos, Dulce Quelhas, Lúcia Lacerda, Elisa Leão Teles, Anthony H. Futerman, Laura Vilarinho, Sandra Alves

“…We demonstrated that this variant induces the skipping of exon 11 leading to a premature stop codon and identified it in NPC patients from two unrelated families. …”
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Identification of Three Novel and One Known Mutation in the WFS1 Gene in Four Unrelated Turkish Families: The Role of Homozygosity Mapping in the Early Diagnosis by Maha Sherif, Hüseyin Demirbilek, Atilla Çayır, Sophia Tahir, Büşra Çavdarlı, Meliha Demiral, Ayşe Nurcan Cebeci, Doğuş Vurallı, Sofia Asim Rahman, Edip Unal, Gönül Büyükyılmaz, Riza Taner Baran, Mehmet Nuri Özbek, Khalid Hussain

“…All mutations were predicted to cause stop codon leading to early termination of protein synthesis and complete loss-of-function. …”
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Catalytic Properties of Caseinolytic Protease Subunit of <i>Plasmodium knowlesi</i> and Its Inhibition by a Member of δ-Lactone, Hyptolide by Cahyo Budiman, Raimalynah Abd Razak, Angelesa Runin Anak Unggit, Rafida Razali, Meiny Suzery, Ruzaidi Azli Mohd Mokhtar, Ping-Chin Lee, Didik Huswo Utomo

“…This study aims to characterize the catalytic properties of ClpP of <i>Plasmodium knowlesi</i> (Pk-ClpP) and the inhibition properties of a δ-lactone hyptolide against this protein. Accordingly, a codon-optimized synthetic gene encoding Pk-ClpP was expressed in <i>Escherichia coli</i> BL21(DE3) and purified under a single step of Ni²⁺-affinity chromatography, yielding a 2.20 mg from 1 L culture. …”
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A Renewed Appreciation of <i>Helicoverpa armigera</i> Nucleopolyhedrovirus BJ (Formerly <i>Helicoverpa assulta</i> Nucleopolyhedrovirus) with Whole Genome Sequencing by Lulu Zhao, Xingjian Liu, Kai Tang, Zhifang Zhang, Huan Zhang, Yinü Li

“…Three of the highest homologs, ORFs with HearNPV, were smaller due to the earlier appearance of the stop codon with unknown functions. P6.9 of HearNPV-BJ, a structural protein, is distinctly different from that of <i>Autographa californica</i> nucleopolyhedrovirus (AcMNPV); its homology with the corresponding gene in HearNPV-C1 was 93.58%. …”
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Comparative Analysis of Chloroplast Genome of <i>Meconopsis</i> (Papaveraceae) Provides Insights into Their Genomic Evolution and Adaptation to High Elevation by Shuqi Zhao, Xiaoman Gao, Xiaolei Yu, Tao Yuan, Guiyu Zhang, Chenlai Liu, Xinzhong Li, Pei Wei, Xiaoyan Li, Xing Liu

“…These cp genomes shared a great deal of similarities in terms of genome size, structure, gene content, GC content, and codon usage patterns. The cp genomes were between 151,864 bp and 154,997 bp in length, and contain 133 predictive genes. …”
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Comparative Chloroplast Genomics of <i>Litsea</i> Lam. (Lauraceae) and Its Phylogenetic Implications by Yunyan Zhang, Yongjing Tian, David Y. P. Tng, Jingbo Zhou, Yuntian Zhang, Zhengwei Wang, Pengfu Li, Zhongsheng Wang

“…The chloroplast genomes of the 12 <i>Litsea</i> species ranged from 152,132 (<i>L. szemaois</i>) to 154,011 bp (<i>L. garrettii</i>) and exhibited a typical quadripartite structure with conserved genome arrangement and content, with length variations in the inverted repeat regions (IRs). No codon usage preferences were detected within the 30 codons used in the chloroplast genomes, indicating a conserved evolution model for the genus. …”
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Spike-Independent Infection of Human Coronavirus 229E in Bat Cells by Marcus G. Mah, Martin Linster, Dolyce H. W. Low, Yan Zhuang, Jayanthi Jayakumar, Firdaus Samsudin, Foong Ying Wong, Peter J. Bond, Ian H. Mendenhall, Yvonne C. F. Su, Gavin J. D. Smith

“…However, one isolate acquired an early stop codon, abrogating spike expression but maintaining infection in bat cells. …”
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A novel compound heterozygous variation in the FKBP10 gene causes Bruck syndrome without congenital contractures: A case report by Liyuan Shang, Weizhe Shi, Yibo Xu, Tianying Nong, Xia Li, Zhaohui Li, Yanhan Liu, Jingchun Li, Ya-Ping Tang, Mingwei Zhu, Hongwen Xu

“…Both variants resulted in a frameshift and premature stop codon. Of these two variants, c.23dupG has not been previously reported. …”
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Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach by Jing Kong, Lin Miao, Zhihui Lu, Shuhui Wang, Baixiang Zhao, Cuiying Zhang, Dongguang Xiao, Desmond Teo, Susanna Su Jan Leong, Adison Wong, Aiqun Yu

“…Results We first validated the in vivo enzymatic activity of a multi-functional amyrin synthase (CrMAS) from Catharanthus roseus, by expressing its codon-optimized gene in Y. lipolytica and assayed for amyrins. …”
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Highly reliable GIGA-sized synthetic human therapeutic antibody library construction by Chao-Yang Huang, Chao-Yang Huang, Ying-Yung Lok, Chia-Hui Lin, Szu-Liang Lai, Yen-Yu Wu, Chih-Yung Hu, Chu-Bin Liao, Chen-Hsuan Ho, Yu-Ping Chou, Yi-Hsuan Hsu, Yu-Hsun Lo, Edward Chern, Edward Chern

“…The engineered antibody sequences were optimized for codon usage and subjected to synthesis. The six CDRs with variable length CDR-H3s were individually subjected to β-lactamase selection and then recombined for library construction. …”
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Heat-induced seizures, premature mortality, and hyperactivity in a novel Scn1a nonsense model for Dravet syndrome by Anat Mavashov, Anat Mavashov, Marina Brusel, Marina Brusel, Jiaxing Liu, Victoria Woytowicz, Haneui Bae, Ying-Hsin Chen, Vardhan S. Dani, Elena Cardenal-Muñoz, Vittoria Spinosa, José Ángel Aibar, Moran Rubinstein, Moran Rubinstein, Moran Rubinstein

“…Our molecular analyses of hippocampal and cortical expression demonstrated that the premature stop codon induced by the R613X mutation reduced Scn1a mRNA and NaV1.1 protein levels to ∼50% in heterozygous Scn1aWT/R613X mice (on either genetic background), with marginal expression in homozygous Scn1aR613X/R613X mice. …”
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Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technolog... by Harald Lahm, Niraj K. Singh, Irina Gottmann, Stefanie A. Doppler, Elda Dzilic, Heike Preisler, Stephanie Schneider, Rüdiger Lange, Markus Krane, Martina Dreßen

“…Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection of specific TBX5-binding sites and their associated genes.…”
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Insights into the in-vitro Susceptibility and Drug&ndash;Drug Interaction Profiles Against Drug-Resistant and Susceptible Mycobacterium tuberculosis Clinical Isolates in Amhara, Et... by Seid A, Girma Y, Dereb E, Kassa M, Nureddin S, Abebe A, Berhane N

“…Five of RIF-resistant isolates (55.6%) carrying rpoB common mutations at codon S450L were associated with high levels of RIF-resistance with MICs of ≥ 2μg/mL, whereas 100% of isolates harboring rpoB substitutions at codons D435V and H445Y were linked with moderate or low-level RIF-resistance in the MIC ranges from 0.5 to 1μg/mL. …”
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Over-Production of the Human SLC7A10 in <i>E. coli</i> and Functional Assay in Proteoliposomes by Michele Galluccio, Tiziano Mazza, Mariafrancesca Scalise, Martina Tripicchio, Martina Scarpelli, Maria Tolomeo, Lorena Pochini, Cesare Indiveri

“…After the testing of several growth conditions, the hASC-1 transporter was successfully expressed in BL21(DE3) codon plus RIL in the presence of 0.5% glucose and induced with 0.05 mM IPTG. …”
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Limited Clinical and Diagnostic Utility of Circulating Tumor DNA Detection in Patients with Early-Stage Well-Differentiated Thyroid Cancer: Comparison with Benign Thyroid Nodules a... by Yong Joon Suh, Mi Jung Kwon, Hye-Mi Noh, Hong Kyu Lee, Yong Joon Ra, Nan Young Kim

“…Although only one patient with an indeterminate lesion on thyroid cytology showed <i>KRAS</i> mutation (codon 146) in the preoperative plasma, that <i>KRAS</i> mutation was not identified in the stage I papillary thyroid carcinoma tissue. …”
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