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4021
A versatile genetic-encoded reporter for magnetic resonance imaging
Published 2023-03-01“…The GEM reporter was developed through codon modification of a bacteria-originated manganese (Mn) binding protein, allowing the sequestration of endogenous Mn in local tissues. …”
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4022
Prediction of RNA editing sites and genome-wide characterization of PERK gene family in maize (Zea mays L.) in response to drought stress
Published 2022-11-01“…These transitions were mostly based on changes in the first and second codon bases. The in-silico expression analysis of transcriptome data revealed the differential expression of ZmPERK genes in response to drought stress and oil content accumulation. …”
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4023
On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes
Published 2022-08-01“…While less frequently reported, specific cases exist in which two overlapping ORFs are in frame and share the stop codon. Here, we studied the occurrence of this genetic arrangement in endolysins, the phage enzymes that cut the bacterial cell wall peptidoglycan to release the virion progeny. …”
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4024
Didemnin B and ternatin-4 differentially inhibit conformational changes in eEF1A required for aminoacyl-tRNA accommodation into mammalian ribosomes
Published 2022-10-01“…Rapid and accurate mRNA translation requires efficient codon-dependent delivery of the correct aminoacyl-tRNA (aa-tRNA) to the ribosomal A site. …”
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4025
Highly rearranged gene orders and rapid evolutionary rates in the mitochondrial genomes of Apodida (Echinodermata: Holothuroidea)
Published 2023-08-01“…Phylogenetic relationships based on two different criteria: (i) nucleotides sequences with all codon positions (PCG123) and (ii) Neutral Transitions Excluded model (NTE) confirmed the monophyly and basal position of the order Apodida with a long branch. …”
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4026
NPC1 silent variant induces skipping of exon 11 (p.V562V) and unfolded protein response was found in a specific Niemann‐Pick type C patient
Published 2020-11-01“…We demonstrated that this variant induces the skipping of exon 11 leading to a premature stop codon and identified it in NPC patients from two unrelated families. …”
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4027
Identification of Three Novel and One Known Mutation in the WFS1 Gene in Four Unrelated Turkish Families: The Role of Homozygosity Mapping in the Early Diagnosis
Published 2021-03-01“…All mutations were predicted to cause stop codon leading to early termination of protein synthesis and complete loss-of-function. …”
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4028
Catalytic Properties of Caseinolytic Protease Subunit of <i>Plasmodium knowlesi</i> and Its Inhibition by a Member of δ-Lactone, Hyptolide
Published 2022-06-01“…This study aims to characterize the catalytic properties of ClpP of <i>Plasmodium knowlesi</i> (Pk-ClpP) and the inhibition properties of a δ-lactone hyptolide against this protein. Accordingly, a codon-optimized synthetic gene encoding Pk-ClpP was expressed in <i>Escherichia coli</i> BL21(DE3) and purified under a single step of Ni<sup>2+</sup>-affinity chromatography, yielding a 2.20 mg from 1 L culture. …”
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4029
A Renewed Appreciation of <i>Helicoverpa armigera</i> Nucleopolyhedrovirus BJ (Formerly <i>Helicoverpa assulta</i> Nucleopolyhedrovirus) with Whole Genome Sequencing
Published 2022-03-01“…Three of the highest homologs, ORFs with HearNPV, were smaller due to the earlier appearance of the stop codon with unknown functions. P6.9 of HearNPV-BJ, a structural protein, is distinctly different from that of <i>Autographa californica</i> nucleopolyhedrovirus (AcMNPV); its homology with the corresponding gene in HearNPV-C1 was 93.58%. …”
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4030
Comparative Analysis of Chloroplast Genome of <i>Meconopsis</i> (Papaveraceae) Provides Insights into Their Genomic Evolution and Adaptation to High Elevation
Published 2024-02-01“…These cp genomes shared a great deal of similarities in terms of genome size, structure, gene content, GC content, and codon usage patterns. The cp genomes were between 151,864 bp and 154,997 bp in length, and contain 133 predictive genes. …”
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4031
Comparative Chloroplast Genomics of <i>Litsea</i> Lam. (Lauraceae) and Its Phylogenetic Implications
Published 2021-06-01“…The chloroplast genomes of the 12 <i>Litsea</i> species ranged from 152,132 (<i>L. szemaois</i>) to 154,011 bp (<i>L. garrettii</i>) and exhibited a typical quadripartite structure with conserved genome arrangement and content, with length variations in the inverted repeat regions (IRs). No codon usage preferences were detected within the 30 codons used in the chloroplast genomes, indicating a conserved evolution model for the genus. …”
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4032
Spike-Independent Infection of Human Coronavirus 229E in Bat Cells
Published 2023-06-01“…However, one isolate acquired an early stop codon, abrogating spike expression but maintaining infection in bat cells. …”
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4033
A novel compound heterozygous variation in the FKBP10 gene causes Bruck syndrome without congenital contractures: A case report
Published 2024-04-01“…Both variants resulted in a frameshift and premature stop codon. Of these two variants, c.23dupG has not been previously reported. …”
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4034
Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach
Published 2022-09-01“…Results We first validated the in vivo enzymatic activity of a multi-functional amyrin synthase (CrMAS) from Catharanthus roseus, by expressing its codon-optimized gene in Y. lipolytica and assayed for amyrins. …”
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4035
Highly reliable GIGA-sized synthetic human therapeutic antibody library construction
Published 2023-04-01“…The engineered antibody sequences were optimized for codon usage and subjected to synthesis. The six CDRs with variable length CDR-H3s were individually subjected to β-lactamase selection and then recombined for library construction. …”
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4036
Heat-induced seizures, premature mortality, and hyperactivity in a novel Scn1a nonsense model for Dravet syndrome
Published 2023-05-01“…Our molecular analyses of hippocampal and cortical expression demonstrated that the premature stop codon induced by the R613X mutation reduced Scn1a mRNA and NaV1.1 protein levels to ∼50% in heterozygous Scn1aWT/R613X mice (on either genetic background), with marginal expression in homozygous Scn1aR613X/R613X mice. …”
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4037
Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technolog...
Published 2024-02-01“…Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection of specific TBX5-binding sites and their associated genes.…”
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4038
Insights into the in-vitro Susceptibility and Drug–Drug Interaction Profiles Against Drug-Resistant and Susceptible Mycobacterium tuberculosis Clinical Isolates in Amhara, Et...
Published 2024-01-01“…Five of RIF-resistant isolates (55.6%) carrying rpoB common mutations at codon S450L were associated with high levels of RIF-resistance with MICs of ≥ 2μg/mL, whereas 100% of isolates harboring rpoB substitutions at codons D435V and H445Y were linked with moderate or low-level RIF-resistance in the MIC ranges from 0.5 to 1μg/mL. …”
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4039
Over-Production of the Human SLC7A10 in <i>E. coli</i> and Functional Assay in Proteoliposomes
Published 2023-12-01“…After the testing of several growth conditions, the hASC-1 transporter was successfully expressed in BL21(DE3) codon plus RIL in the presence of 0.5% glucose and induced with 0.05 mM IPTG. …”
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4040
Limited Clinical and Diagnostic Utility of Circulating Tumor DNA Detection in Patients with Early-Stage Well-Differentiated Thyroid Cancer: Comparison with Benign Thyroid Nodules a...
Published 2021-04-01“…Although only one patient with an indeterminate lesion on thyroid cytology showed <i>KRAS</i> mutation (codon 146) in the preoperative plasma, that <i>KRAS</i> mutation was not identified in the stage I papillary thyroid carcinoma tissue. …”
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