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4121
Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection
Published 2023-11-01“…Here, a recombinant chimpanzee adenovirus-based vaccine (AdC68-G) and a DNA vaccine (DNA-G) were developed by expressing the codon-optimized full-length glycoprotein (G) of NiV. …”
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4122
Cohort analysis of novel SPAST variants in SPG4 patients and implementation of in vitro and in vivo studies to identify the pathogenic mechanism caused by splicing mutations
Published 2023-12-01“…In contrast, the splicing variant c.1005-1delG differed from what was predicted (skipping exon 7), and the functional study indicates the loss of frame and formation of a premature stop codon. The present study evidenced the high splice variants in SPG4 patients and indicated the relevance of functional assays added to in silico analysis to decipher the pathogenic mechanism.…”
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4123
RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma
Published 2024-03-01“…Mechanically, NSUN6 mediated tRNA m5C modifications selectively enhance the translation efficiency of CDH1 mRNA in a codon dependent manner. Rescue assays revealed that E-cadherin is an essential downstream target that mediates NSUN6’s function in the regulation of ESCC progression. …”
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4124
CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO2-Fixing Methanogenic Archaeon
Published 2022-08-01“…Mutations were introduced into an archaeal promoter BRE (transcription factor B [TFB] recognition element), a terminator U-tract region, and a gene coding region. Stop codon introduction into a gene through single-nucleotide substitution shut down its expression, providing an alternative strategy for gene inactivation. …”
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4125
Genomic Analysis of Ceftazidime/Avibactam-Resistant GES-Producing Sequence Type 235 <i>Pseudomonas aeruginosa</i> Isolates
Published 2022-06-01“…WGS revealed the presence of two mutations within the <i>bla</i><sub>GES-20</sub> gene comprising two single-nucleotide substitutions, which caused aspartic acid/serine and leucine/premature stop codon amino acid changes at positions 165 (D165S) and 237 (L237X), respectively. …”
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4126
The Cancer Driver Genes IDH1 and IDH2 and CD204 in WHO-Grade 4 Astrocytoma: Crosstalk Between Cancer Metabolism and Tumour Associated Macrophage Recruitment in Tumour Microenvironm...
Published 2023-02-01“…The alteration hotspot in codon IDH1R132 and IDH2R172 was examined using direct sequencing. …”
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4127
Effects of <i>Bt-Cry1Ah1</i> Transgenic Poplar on Target and Non-Target Pests and Their Parasitic Natural Enemy in Field and Laboratory Trials
Published 2020-11-01“…We generated a <i>Bt-Cry1Ah1</i> gene incorporating codon optimization and transferred it into <i>Populus deltoides</i> × <i>P. euramericana</i> cv “Nanlin895” using an Agrobacterium-mediated method. …”
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4128
Mapping-by-sequencing using NGS-based 3′-MACE-Seq reveals a new mutant allele of the essential nodulation gene Sym33 (IPD3) in pea (Pisum sativum L.)
Published 2019-04-01“…Sequencing of the Sym33 allele of the N24 (sym11) mutant revealed a single nucleotide deletion (c.C319del) in its third exon resulting in a codon shift in the open reading frame and premature translation termination. …”
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4129
Aflatoxin B1: Mechanism of mutagenesis
Published 2007-02-01“…AFB1 exposure is also associated with a specific codon 249 AGG (arginine) to AGT (serine) mutation in the p53 tumor suppressor gene that inactivates the protein. …”
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4130
Plastome evolution and phylogenomics of Trichosporeae (Gesneriaceae) with its morphological characters appraisal
Published 2023-05-01“…The RSCU and ENC values indicated that the codon usage pattern was conserved in Trichosporeae. …”
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4131
Comparative analyses and phylogenetic relationships of thirteen Pholidota species (Orchidaceae) inferred from complete chloroplast genomes
Published 2023-05-01“…The annotation contained a total of 135 genes in each cp. genome, i.e., 89 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The codon usage analysis indicated the preference of A/U-ending codons. …”
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4132
The exon-skipping oligonucleotide, KitStop, depletes tissue-resident mast cells in vivo to ameliorate anaphylaxis
Published 2023-01-01“…KitStop administration results in the integration of a premature stop codon within the mRNA transcript of the KIT receptor—a receptor tyrosine kinase found primarily on mast cells and whose gain-of-function mutation can lead to systemic mastocytosis. …”
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4133
The chloroplast genome of Farsetia hamiltonii Royle, phylogenetic analysis, and comparative study with other members of Clade C of Brassicaceae
Published 2022-08-01“…The genomes of F. hamiltonii and F. occidentalis show shared amino acid frequencies and codon use, RNA editing sites, simple sequence repeats, and oligonucleotide repeats. …”
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4134
Revisiting chloroplast genomic landscape and annotation towards comparative chloroplast genomes of Rhamnaceae
Published 2023-01-01“…The clustering based on codon usage was congruent with the molecular phylogenetic tree. …”
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4135
Complete Chloroplast Genomes and the Phylogenetic Analysis of Three Native Species of Paeoniaceae from the Sino-Himalayan Flora Subkingdom
Published 2023-12-01“…In addition, the genomes had 61–64 SSRs, with mononucleotide repeats being the most abundant. The codon bias patterns of the three species tend to use codons ending in A/U. …”
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4136
The fluorescent protein iLOV as a reporter for screening of high‐yield production of antimicrobial peptides in Pichia pastoris
Published 2022-07-01“…An easy and simple medium‐throughput plate‐based screen directly following transformation is demonstrated for low complexity screening, while a high‐throughput method using fluorescence‐activated cell sorting (FACS) allowed for comprehensive library screening. Both codon optimization of the iLOV_NI01 fusion cassettes and different integration strategies into the P. pastoris genome were tested to produce and isolate a high‐yielding strain. …”
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4137
Developing genome-editing strategies to ameliorate HbE/beta-thalassaemia
Published 2021“…Next base editors, a modular variant of the CRISPR/Cas9 system, were used to repair the pathogenic codon 26 mutation in the HBB gene that causes HbE/β-thalassaemia. …”
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4138
Vocal Cord Paralysis and Feeding Difficulties as Early Diagnostic Clues of Congenital Myasthenic Syndrome with Neonatal Onset: A Case Report and Review of Literature
Published 2023-05-01“…In particular, the c.565C>T variant is novel and has never been described in the literature; it causes the insertion of a premature stop codon (p.Arg189Ter) likely leading to a consequent formation of a truncated nonfunctioning protein. …”
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4139
Targeted and selective knockout of the TLQP-21 neuropeptide unmasks its unique role in energy homeostasis
Published 2023-10-01“…To achieve this goal, we used a knowledge-based approach by mutating a codon in the Vgf sequence leading to the substitution of the C-terminal Arginine of TLQP-21, which is the pharmacophore as well as an essential cleavage site from its precursor, into Alanine (R21→A). …”
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4140
Molecular and hematological studies in a cohort of beta zero South East Asia deletion (β°-thal SEA) from Malaysian perspective
Published 2022-11-01“…The mean Hb for heterozygous β°-thal SEA deletion (13.44 ± 1.45 g/dl) was normal and significantly higher than heterozygous IVS 1-1 and Codon 41/42 (post hoc test, p < 0.05). The medians for the MCV and MCH of β°-thal SEA deletion were significantly higher than for all heterozygote β°-thalassaemia traits (Mann Whitney test, p < 0.05). …”
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