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1461
Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant
Published 2023-02-01“…Abstract The single-nucleotide polymorphism (SNP) of p53, in particular the codon 72 variants, has recently been implicated as a critical regulator in tumor progression. …”
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1462
Characterization of Four Complete Mitogenomes of <i>Monolepta</i> Species and Their Related Phylogenetic Implications
Published 2024-01-01“…The substitution saturation of three datasets (13 <i>PCGs</i>_codon1, 13 <i>PCGs</i>_codon2, 13 <i>PCGs</i>_codon3) showed that there was no saturation across all datasets. …”
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1463
Estimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deer
Published 2023-12-01“…Three single nucleotide polymorphisms (SNPs) were found in red deer – codon A136A, codon T98A, codon Q226E – and no sequence variation was detected in fallow deer. …”
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1464
Detection of variant of hepatitis B virus and beta-globin through DNA technology
Published 2009“…Three PCR-based methods were employed to detect the HBV precore stop codon mutant. They are RG-PCR, SSCP analysis and non-radioactive direct DNA cycle sequencing.…”
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1465
The First Mitogenomes of the Subfamily Odontiinae (Lepidoptera, Crambidae) and Phylogenetic Analysis of Pyraloidea
Published 2021-05-01“…Most PCGs are initiated with an ATN codon and terminated by a codon of TAR. All tRNAs could be folded into the clover-leaf structure with the exception of trnS1 (AGN), in which the dihydrouridine (DHU) arm formed a simple loop, and the motif ‘ATAG’ and ‘ATTTA’ in the A + T-rich region was also founded. …”
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1466
Technical Data of Heterologous Expression and Purification of SARS-CoV-2 Proteases Using <i>Escherichia coli</i> System
Published 2021-09-01“…Meanwhile, PLpro-CoV2 was successfully expressed in a fully soluble form in either BL21(DE3) or BL21-CodonPlus(DE3) strains. In contrast, the single affinity chromatography step was only applicable for PLpro-CoV2 expressed using <i>E. coli</i> BL21-CodonPlus(DE3) at 18 or 37 °C, with a yield and purification fold of 86% (18 °C) or 83.36% (37 °C) and 112 (18 °C) or 71 (37 °C), respectively. …”
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1467
A novel autism-associated UBLCP1 mutation impacts proteasome regulation/activity
Published 2023-12-01“…The deletion in UBLCP1 exon 10 (g.158,710,261CAAAG > C) generates a premature stop codon interrupting the phosphatase domain and is predicted as pathogenic. …”
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1468
The Characterization and Phylogenetic Implications of the Mitochondrial Genomes of <i>Antheminia varicornis</i> and <i>Carpocoris purpureipennis</i> (Hemiptera: Pentatomidae)
Published 2023-12-01“…Nucleotide composition is highly biased with the third codon in PCGs displaying the highest A + T content. …”
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1469
Understanding protein diffusion on force-induced stretched DNA conformation
Published 2022-12-01“…The functional role of such a DNA base triplet was hypothesized to be coupled with the ease of recognition of DNA bases by DNA-binding proteins (DBPs) and the physical origin of three letters (codon/anti-codon) in the genetic code. However, the underlying mechanism of base-triplet formation and the ease of DNA base-pair recognition by DBPs remain elusive. …”
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1470
Two New Variants in FYCO1 Are Responsible for Autosomal Recessive Congenital Cataract in Iranian Population
Published 2022-09-01“…Apreviously reported missense mutation, c.265C>T (p.Arg89Cys), was found in one Iranian family for the first time,and a combination of two variants in a single codon, c.[265C>T;267C>A] (p.Arg89X), was identified in the three otherfamilies. …”
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1471
Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
Published 2014-05-01“…Percentage of codon having high-frequency distribution was improved to 67%. …”
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1472
Comparison of chloroplast genomes and phylogenomics in the Ficus sarmentosa complex (Moraceae).
Published 2022-01-01“…The number of simple sequence repeat (SSR) loci varied from 175 (F. dinganensis and F. howii) to 190 (F. polynervis), with the dinucleotide motif appearing the most frequently. Relative synonymous codon usage (RSCU) analysis indicated that codons ending with A/T were prior to those ending with C/T. …”
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1473
Variability in prion protein genotypes by spatial unit to inform susceptibility to chronic wasting disease
Published 2022-12-01“…We sequenced the prion protein gene (PRNP) of 565 elk samples collected over 7 years (2014–2020) and found two polymorphic sites (codon 21 and codon 132). The allele associated with reduced susceptibility to CWD is present in the population, and there was no evidence of deviations from Hardy-Weinberg equilibrium in any of our sampling years (p-values between 0.14 and 1), consistent with the lack of selective pressure on the PRNP. …”
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1474
Design of Adenovirus 5 Vector with Adenovirus 26 Hexon Hypervariable Region Sequence using In Silico Approach
Published 2023-01-01“…The splice site prediction of Ad5HVR26 chimera vector was found at HVR5, 6, and 7. The codon change in the splice site was performed to decrease the possibility of incorrect splicing, while retaining the original amino acid sequence. …”
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1475
Bacteriophage Infection of the Marine Bacterium <i>Shewanella glacialimarina</i> Induces Dynamic Changes in tRNA Modifications
Published 2023-01-01“…Furthermore, we observe a preference for the UAC codon in viral transcripts expressed at the late stage of infection, which coincides with an increase in queuosine modification. …”
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1476
Antimicrobial resistance of Mycobacterium tuberculosis clinical isolates from patients with tuberculous spondylitis
Published 2023-12-01“…For rifampicin and isoniazid, the dominant mutations associated with resistance were: serine replacement for leucine in the 531 codon of the rpoB gene for rifampicin and serine replacement for threonine in the 315 codon of the katG gene for isoniazid. …”
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1477
Assessing Mutations in Treatment Response-related Genes in Egyptian Patients with Non-small Cell Lung Cancer
Published 2022-05-01“…EGFR mutations were detected in 22.3% (12.2% in exon 19, 8.6% in exon 21, and 1.4% in exon 18) and K-RAS mutations in 17.3% (15.8% in codon 12 and 1.4% in codon 13), whereas combined mutations were detected in nine patients (6.5%). …”
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1478
Mitochondrial genome features and systematic evolution of diospyros kaki thunb 'Taishuu'
Published 2024-03-01“…There are four types of stop codons: CGA, TAA, TAG, and TGA, with TAA being the most frequently used stop codon (45.24%). …”
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1479
The complete mitochondrial genome of Gobio huanghensis (Cypriniformes: Cyprinidae) in the Yellow River, China
Published 2024-02-01“…Notably, COX1 initiates with the start codon GTG, distinct from the typical ATG start codon of other PCGs. …”
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1480
Whole-Genome Sequencing of Rice Mutant Library Members Induced by N-Methyl-N-Nitrosourea Mutagenesis of Fertilized Egg Cells
Published 2022-07-01“…Although this mutant library was a small population, nonsynonymous mutations were found in nearly 61% of all annotated rice genes, and 8.6% (3248 genes) had point mutations with large effects on gene function, such as gaining a stop codon or losing a start codon. WGS showed MNU-mutagenesis using rice fertilized egg cells induces mutations efficiently and is suitable for constructing mutant libraries for an in silico mutant screening system. …”
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