Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing, including a splice-site mutation in nucleoredoxin. by Melissa K Boles, Bonney M Wilkinson, Laurens G Wilming, Bin Liu, Frank J Probst, Jennifer Harrow, Darren Grafham, Kathryn E Hentges, Lanette P Woodward, Andrea Maxwell, Karen Mitchell, Michael D Risley, Randy Johnson, Karen Hirschi, James R Lupski, Yosuke Funato, Hiroaki Miki, Pablo Marin-Garcia, Lucy Matthews, Alison J Coffey, Anne Parker, Tim J Hubbard, Jane Rogers, Allan Bradley, David J Adams, Monica J Justice

“…The other 61% lie in noncoding regions, many of them in highly conserved sequences. A lesion in the perinatal lethal line l11Jus13 alters a consensus splice site of nucleoredoxin (Nxn), inserting 10 amino acids into the resulting protein. …”
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Detection and Genetic Characterization of Astroviruses in Brain Tissues of Wild Raccoon Dogs by Su-Beom Chae, Chang-Gi Jeong, Jun-Soo Park, Eun-Jee Na, Jae-Ku Oem

“…Phylogenetic and capsid protein amino acid sequence analysis of raccoon dog AstVs detected in the brain the 17-148B strain belonging to the HMO clade and exhibiting conserved sequences found in neurotropic AstVs (NT-AstVs), indicating their potential as NT-AstVs. …”
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Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips by Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong

“…Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42°C for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. …”
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection by Mehrdad Ravanshad, Z Khanlari, M Rasouli, M Ziyaeyan, S Falahi

“…Materials and Methods: Conserved sequences were selected for design of primers. …”
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In silico comparative structural and functional analysis of arsenite methyltransferase from bacteria, fungi, fishes, birds, and mammals by Ashutosh Kabiraj, Anubhab Laha, Anindya Sundar Panja, Rajib Bandopadhyay

“…Conclusion All of our in silico studies confirmed the fact that arsenite methyltransferase is a cytosolic stable enzyme with conserved sequences over a wide range of organisms. Thus, because of its stable and ubiquitous nature, arsenite methyltransferase could be employed in arsenic bioremediation.…”
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Isolation and functional characterization of bidirectional promoters in rice by Rui eWang, Yan eYan, Menglin eZhu, Mei eYang, Fei eZhou, Hao eChen, Yongjun eLin

“…Our study proposes a feasible method for selecting, cloning and functionally identifying bidirectional promoters as well as for discovering their bidirectional regulatory regions and conserved sequences in rice.…”
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An efficient genetic algorithm for structural RNA pairwise alignment and its application to non-coding RNA discovery in yeast by Taneda Akito

“…By focusing our search to the relatively short regions (50 bp to 2,000 bp) sandwiched by conserved sequences, we successfully predict 714 intergenic and 1,311 sense or antisense ncRNA candidates, which were found in the pairwise alignments with stable consensus secondary structure and low sequence identity (≤ 50%). …”
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Whole Genome Sequence Analysis of a Prototype Strain of the Novel Putative Rotavirus Species L by Reimar Johne, Katja Schilling-Loeffler, Rainer G. Ulrich, Simon H. Tausch

“…The non-coding genome segment termini of RVL showed conserved sequences at the 5′-end (positive-sense RNA strand), which are common to all rotaviruses, and those conserved among the RVB-like clade at the 3′-end. …”
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Prophage-mediated dynamics of 'Candidatus Liberibacter asiaticus' populations, the destructive bacterial pathogens of citrus huanglongbing. by Lijuan Zhou, Charles A Powell, Wenbin Li, Mike Irey, Yongping Duan

“…Among them, Types A and B share highly conserved sequences and localize within the two prophages, FP1 and FP2, respectively. …”
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Functional analysis of three new alpha-thalassemia deletions involving MCS-R2 reveals the presence of an additional enhancer element in the 5' boundary region. by Serena Capasso, Giovanna Cardiero, Gennaro Musollino, Romeo Prezioso, Rosario Testa, Sabrina Dembech, Giulio Piluso, Vincenzo Nigro, F Anna Digilio, Giuseppina Lacerra

“…We report three novel deletions involving the Multispecies Conserved Sequences (MCS) R2, also known as the Major Regulative Element (MRE), in patients showing the α-thalassemia phenotype. …”
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The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. by Takashi Shibano, Hiroshi Mamada, Fumihiko Hakuno, Shin-Ichiro Takahashi, Masanori Taira

“…The inner nuclear membrane (INM) protein Nemp1/TMEM194A has previously been suggested to be involved in eye development in Xenopus, and contains two evolutionarily conserved sequences in the transmembrane domains (TMs) and the C-terminal region, named region A and region B, respectively. …”
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Interplay between chaperones and protein disorder promotes the evolution of protein networks. by Sebastian Pechmann, Judith Frydman

“…We further find that λ serves well to quantify the evolution of intrinsically disordered proteins even though the unstructured, thus generally variable regions in proteins are often flanked by very conserved sequences. Crucially, we show that both intrinsically disordered proteins and highly re-wired proteins in protein interaction networks, which have evolved new interactions and functions, exhibit a higher λ at the expense of enhanced chaperone assistance. …”
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Functional evolution of cis-regulatory modules at a homeotic gene in Drosophila. by Margaret C W Ho, Holly Johnsen, Sara E Goetz, Benjamin J Schiller, Esther Bae, Diana A Tran, Andrey S Shur, John M Allen, Christoph Rau, Welcome Bender, William W Fisher, Susan E Celniker, Robert A Drewell

“…Bioinformatic analysis reveals the presence of short conserved sequences within defined CRMs, representing gap and pair-rule transcription factor binding sites. …”
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Molecular cloning and expression analysis of the STAT1 gene from olive flounder, <it>Paralichthys olivaceus</it> by Chung Jongkyeong, Kim GunDo, Seo Jung, Kang Jung-Ha, Park Eun-Mi, Choi Tae-Jin

“…</p> <p>Results</p> <p>A DNA fragment containing the conserved SH2 domain was amplified by RT-PCR using degenerate primers designed based on the highly conserved sequences in the SH2 domains of the zebrafish and mammalian STAT1. …”
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Murasaki: a fast, parallelizable algorithm to find anchors from multiple genomes. by Kris Popendorf, Hachiya Tsuyoshi, Yasunori Osana, Yasubumi Sakakibara

“…When large-scale rearrangements break the collinearity of gene orders among genomes, genome comparison algorithms must first identify sets of short well-conserved sequences present in each genome, termed anchors. …”
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Gene Identification, expression analysis and molecular docking of ATP sulfurylase in the selenization pathway of Cardamine hupingshanensis by Zhijing Xiao, Yanke Lu, Yi Zou, Chi Zhang, Li Ding, Kai Luo, Qiaoyu Tang, Yifeng Zhou

“…Nine ATPS genes were found from C. hupingshanensis, which share highly conserved sequences with ATPS from Arabidopsis thaliana. …”
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External Quality Assessment for the Detection of Measles Virus by Reverse Transcription-PCR Using Armored RNA. by Dong Zhang, Yu Sun, Tingting Jia, Lei Zhang, Guojing Wang, Rui Zhang, Kuo Zhang, Guigao Lin, Jiehong Xie, Lunan Wang, Jinming Li

“…The external quality assessment panel, which consisted of 10 specimens, was prepared using armored RNAs, complex of noninfectious MS2 bacteriophage coat proteins encapsulated RNA of measles virus, as measles virus surrogate controls. Conserved sequences amplified from a circulating measles virus strain or from a vaccine strain were encapsulated into these armored RNAs. …”
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Comparison of the Mitochondrial Genomes and Steady State Transcriptomes of Two Strains of the Trypanosomatid Parasite, Leishmania tarentolae. by Larry Simpson, Stephen M Douglass, James A Lake, Matteo Pellegrini, Feng Li

“…Minicircles were identified by a characteristic size, the presence of three short conserved sequences, a region of inherently bent DNA and the presence of single gRNA genes at a fairly defined location. …”
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Cloning and Expression Profiling of the Polycomb Gene, Protein from Tomato L. by Zainab M. Almutairi, Monther T. Sadder

“…In this study, we present the cloning and genomic structure of a putative SlRBR gene in the tomato Solanum lycopersicum L. by isolating cDNA clones that correspond to the SlRBR gene from tomato using primers that were designed from available Solanaceae ESTs based on conserved sequences between the PcG genes in Arabidopsis thaliana and tomato. …”
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Conserved intron positions in ancient protein modules by de Roos Albert DG

“…However, no apparent differences in phase distribution were found between introns in conserved versus non-conserved sequences.</p> <p>Conclusion</p> <p>The data confirm an origin of introns deep in the eukaryotic branch and is in concordance with the presence of introns in the first functional protein modules in an 'Exon theory of genes' scenario. …”
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