The Pkn22 Kinase of Nostoc PCC 7120 Is Required for Cell Differentiation via the Phosphorylation of HetR on a Residue Highly Conserved in Genomes of Heterocyst-Forming Cyanobacteri... by Baptiste Roumezi, Xiaomei Xu, Véronique Risoul, Yingping Fan, Régine Lebrun, Amel Latifi

“…The Serine 130 residue is part of a highly conserved sequence in filamentous cyanobacterial strains differentiating heterocysts. …”
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Genetic evaluation and germplasm identification analysis on ITS2, trnL-F, and psbA-trnH of alfalfa varieties germplasm resources by Yong Wang, Chunbo Xu, Laga Tong, Xiaoming Zhang, Mingjiu Wang

“…Alfalfa cultivars trnL-F and psbA-trnH sequences have apparent differences, showing that chloroplast conservative sequences were independent evolution. Compared with trnL-F and psbA-trnH sequences of alfalfa cultivars, psbA-trnH sequence has abundant variation sites and can better reflect the differences between cultivars than the trnL-F sequence. …”
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Construction of Lipopolysaccaride Binding Protein Knockout Mice Using CRISPR/Cas9 Technology by LI Sidi, FU Bin, GUO Zhongkun, LIN Yingjie, ZHANG Zhenyu, MI Chuanliang, WANG Kezhou

“…ObjectiveTo construct a stable hereditary lipopolysaccaride binding protein (Lbp) gene knockout mice by using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) gene editing technology.MethodsAccording to the sequence characteristics of Lbp gene in C57BL/6J mice, the target of sgRNA was designed, the 5'-end protein coding conserved sequence of Lbp gene was deleted and the shift mutation was introduced to inactivate LBP. …”
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Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses by Zongshu Zhang, Chunguang Wang, Xi Chen, Zichuang Zhang, Guoqiang Shi, Xianghe Zhai, Tie Zhang

“…In this study, specific primers and CRISPR RNA (crRNA) were designed according to the conservative sequence of AIV Nucleprotein (NP) gene. RAA technology was used to amplify the target sequence, and the amplification products were visually detected by lateral flow dipstick (LFD). …”
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A Conserved Female-Specific Requirement for the <i>GGT</i> Gene in Mosquito Larvae Facilitates RNAi-Mediated Sex Separation in Multiple Species of Disease Vector Mosquitoes by Keshava Mysore, Longhua Sun, Ping Li, Joseph B. Roethele, Joi K. Misenti, John Kosmach, Jessica Igiede, Molly Duman-Scheel

“…Yeast targeting a conserved sequence in <i>Culex GGT</i> genes was incorporated into a larval mass-rearing diet, permitting the generation of fit adult male <i>C. pipiens</i> and <i>C. quinquefasciatus</i>, two species for which labor-intensive manual sex separation had previously been utilized. …”
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Characterization of a novel psyllid-transmitted waikavirus in carrots by Ariel Shushan, Neta Luria, Oded Lachman, Noa Sela, Orly Laskar, Eduard Belausov, Elisheva Smith, Aviv Dombrovsky

“…Aligning the waikavirus-deduced amino-acid sequence with other waikavirus species at the Pro-Pol region, a conserved sequence between the putative proteinase and the RNA-dependent RNA polymerase, showed a ∼40% identity, indicating the identification of a new waikavirus species. …”
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Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC) by Zhen He, Shuangyu Sheng, Lingqi Wang, Tingting Dong, Kun Zhang, Liangjun Li

“…In order to assess the efficacy of gene silencing, we also cloned conserved sequence of gibberellin insensitive dwarf (GID1) cDNA sequences into the vector and inoculated the water dropwort. …”
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The complete mitochondrial genome of Rhynchocypris oxycephalus (Teleostei: Cyprinidae) and its phylogenetic implications by Zhichao Zhang, Qiqun Cheng, Yushuang Ge

“…The control region is 943bp in length, with a termination‐associated sequence, six conserved sequence blocks (CSB‐1, CSB‐2, CSB‐3, CSB‐D, CSB‐E, CSB‐F), and a repetitive sequence. …”
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Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme by Bareket Dassa, Ilya Borovok, Vincent Lombard, Bernard Henrissat, Raphael Lamed, Edward A. Bayer, Sarah Moraïs

“…We explored the genomic organization of key cellulosome components by comparing the cellulosomal gene clusters in each bacterial species, and the conserved sequence features of the specific cellulosomal modules (cohesins and dockerins), on the background of their phylogenetic relationship. …”
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Genome-wide identification and function analysis of HMAD gene family in cotton (Gossypium spp.) by Qinqin Wang, Xuke Lu, Xiugui Chen, Lanjie Zhao, Mingge Han, Shuai Wang, Yuexin Zhang, Yapeng Fan, Wuwei Ye

“…In addition, 15 genes were lost during evolution. Furthermore, conserved sequence analysis found the conserved catalytic center containing an anion binding (CXXC) box. …”
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Molecular Identification and Intra-species Variations among Leishmania infantum Isolated from Human and Canine Visceral Leishmaniasis in Iran by Abdolhossein DALIMI, Anita MOHAMMADIHA, Mehdi MOHEBALI, Asad MIRZAEI, Mohammadreza MAHMOUDI

“…DNA was confirmed by amplification of variable area of the minicircle kDNA (conserved sequence blocks (CSB)). Conclusion: Low divergence and high likelihood were seen among L. infantum isolates of human and dogs from Iran with a very slight divergence was seen between isolates from northwest and south of Iran, thus grouped in a unique clad. …”
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Screening of small molecular compounds with carcinogenic inhibition function of HPV-16 E6 by Jiaoyu He, Qiufu Li, Yang Liu, Tianjun Li, Chunlan cheng, Ning Li, Yanru Cui, Yunfan Shi, Yiran Liu, Xia Wei, Xianping Ding

“…HPV persistent infection and cell immortalization does not always cause cancer, but they increase risk and can lead to tumor formation and carcinogenesis in cooperation with other tumorigenic factors, and HR-HPV E6 oncoprotein is the mainly tumorigenic factors.E6 binds to the highly conserved sequence “LXXLL” of E6AP to form a heterodimer, which recruits and induces the degradation of tumor suppressor protein p53, trigger the immortalized transformation of infected cells, and induce its carcinogenicity. …”
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The Regulatory Functions of σ⁵⁴ Factor in Phytopathogenic Bacteria by Chao Yu, Fenghuan Yang, Dingrong Xue, Xiuna Wang, Huamin Chen

“…RpoN directly binds to the highly conserved sequence, GGN₁₀GC, at the −24/−12 position relative to the transcription start site of target genes. …”
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Hnf1b renal expression directed by a distal enhancer responsive to Pax8 by L. Goea, I. Buisson, V. Bello, A. Eschstruth, M. Paces-Fessy, R. Le Bouffant, A. Chesneau, S. Cereghini, J. F. Riou, M. Umbhauer

“…Using phylogenetic footprinting, we identified an evolutionary conserved sequence (CNS1) located several kilobases (kb) upstream the Hnf1b transcription start and harboring epigenomic marks characteristics of a distal enhancer in embryonic and adult renal cells in mammals. …”
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Genomic positional conservation identifies topological anchor point RNAs linked to developmental loci by Paulo P. Amaral, Tommaso Leonardi, Namshik Han, Emmanuelle Viré, Dennis K. Gascoigne, Raúl Arias-Carrasco, Magdalena Büscher, Luca Pandolfini, Anda Zhang, Stefano Pluchino, Vinicius Maracaja-Coutinho, Helder I. Nakaya, Martin Hemberg, Ramin Shiekhattar, Anton J. Enright, Tony Kouzarides

“…Furthermore, we find that tapRNAs contain conserved sequence domains that are enriched in motifs for zinc finger domain-containing RNA-binding proteins and transcription factors, whose binding sites are found mutated in cancers. …”
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Identification of 2,4-Di-<i>tert</i>-butylphenol as a Novel Agonist for Insect Odorant Receptors by Shinhui Lee, Sanung Eom, Minsu Pyeon, Myungmi Moon, Jihwon Yun, Jaehyeong Lee, Yong-Seok Choi, Junho H. Lee

“…Orco, an OR coreceptor, is crucial for olfactory transduction, as it possesses a conserved sequence across the insect lineage. In this study, we focused on 2,4-di-<i>tert</i>-butylphenol (DTBP), a single substance present in acetic acid bacteria culture media. …”
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Genome-wide in silico and in vitro mining to develop a novel cyclotide-based marker system in plants by Arwa Salah, Eman Abdelsattar El-Khateeb, Reda Mohamed Gaafar, Mohamed Atia Mohamed Atia

“…Here we developed four degenerate cyclotide subfamily-specific (CSS) markers based on their subfamily’s semi-conserved sequence for identifying each subfamily separately. …”
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Phylogenetic Characterisation of the Full Genome of a Bagaza Virus Isolate from Bird Fatalities in South Africa by Adriano Mendes, Olivia Lentsoane, Mushal Allam, Zamantungwaka Khumalo, Arshad Ismail, Jacobus A. W. Coetzer, Marietjie Venter

“…The 3′UTR sequence of ZRU96-16 was resolved with sufficient detail to be able to annotate the variable and conserved sequence elements within this region. Multiple sequence alignment of the 3′UTR suggested that it could be useful in lineage designation as more similar viruses carried similar mutations across this region, while also retaining certain unique sites. …”
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The role of IBV PL1pro in virus replication and suppression of host innate immune responses by Weirong Liu, Ge Mu, Yiquan Jia, Mengting Yu, Songbai Zhang, Zhen Wang, Shouguo Fang

“…Results The recombinant viruses rIBV-ΔPL1pro and rIBV-ΔPL1pro-N were obtained using reverse genetic techniques through the deletion of the IBV PL1pro domain and the N-terminal conserved sequence of PL1pro (PL1pro-N). We observed significantly lower replication of rIBV-ΔPL1pro and rIBV-ΔPL1pro-N than wild-type IBV. …”
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Co-silencing of tomato S-adenosylhomocysteine hydrolase genes confers increased immunity against Pseudomonas syringae pv. tomato DC3000 and enhanced tolerance to drought stress by Li Xiao Hui, Lei eHuang, Yongbo eHong, Yafen eZhang, Shixia eLiu, Zhang Hui Juan, Dayong eLi, Fengming eSong

“…However, co-silencing of three SlSAHH genes using a conserved sequence led to significant inhibition of vegetable growth. …”
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