Structural and Chemical Biology Approaches Reveal Isoform-Selective Mechanisms of Ligand Interactions in Mammalian Cryptochromes by Simon Miller, Tsuyoshi Hirota

“…Accumulating evidence for distinct roles of CRY1 and CRY2 has highlighted the need for CRY isoform-selective regulation; however, highly conserved sequences in CRY ligand-binding sites have hindered the design of isoform-selective compounds. …”
Get full text

Picocyanobacterial community structure and space-time dynamics in the subalpine Lake Maggiore (N. Italy) by Gianluca Corno, Roberto Bertoni, Emanuele Caravati, Cristiana Callieri

“…We investigated the community structure of freshwater picocyanobacteria (Pcy) in the oligotrophic subalpine Lake Maggiore (Northern Italy) using the least conserved sequences of the Internal Transcribed Spacer (ITS-1) fragment. …”
Get full text

Functional single nucleotide polymorphism-based association studies by Carlton Victoria EH, Ireland James S, Useche Francisco, Faham Malek

“…SNP genotyping technologies are already sufficient for such studies, but studies will require continued genomic sequencing of multiple species, research on the functional role of conserved sequences and additional SNP discovery and validation efforts (including targeted SNP discovery to identify the rare alleles in functional regions). …”
Get full text

Conservation of expression and sequence of metabolic genes is reflected by activity across metabolic states. by Yonatan Bilu, Tomer Shlomi, Naama Barkai, Eytan Ruppin

“…In addition, we show that genes that are active in a large fraction of the space of optimal solutions tend to have conserved sequences. This supports the possibility that there is less selective pressure to maintain genes that are relevant for only a small number of metabolic states.…”
Get full text

Intrahost variations in the envelope receptor-binding domain (RBD) of HTLV-1 and STLV-1 primary isolates by Sitbon Marc, Battini Jean-Luc, Gallego Sandra, Gessain Antoine, Lavanya Madakasira, Kim Felix J, Courgnaud Valérie

“…The PTLV envelope glycoprotein surface component (SUgp46) comprises a receptor-binding domain (RBD) that alternates hypervariable and highly conserved sequences. To further delineate highly conserved motifs in PTLV RBDs, we investigated the intrahost variability of HTLV-1 and STLV-1 by generating and sequencing libraries of DNA fragments amplified within the RBD of the SUgp46 <it>env </it>gene. …”
Get full text

Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster. by Flint, J, Tufarelli, C, Peden, J, Clark, K, Daniels, R, Hardison, R, Miller, W, Philipsen, S, Tan-Un, K, McMorrow, T, Frampton, J, Alter, B, Frischauf, A, Higgs, D

“…However, refining these methods by ranking identity scores of non-coding sequences, we found conserved sequences including the previously characterized alpha globin major regulatory element. …”

Identification of an MCM4 homologue expressed specifically in the sexual stage of Plasmodium falciparum. by Li, J, Cox, L

“…The encoded protein, termed PfMCM4, contains all conserved sequences in the MCM family and displays the highest homology to the Cdc54 (MCM4) of Saccharomyces cerevisiae. …”

A 2-Mb YAC contig encompassing three loci (DXF34, DXS14, and DXS390) that lie between Xp11.2 translocation breakpoints associated with incontinentia pigmenti type 1. by Reed, V, Rider, S, Maslen, G, Hatchwell, E, Blair, H, Uwechue, I, Craig, I, Laval, S, Monaco, A, Boyd, Y

“…This expansion of the cloned region around DXF34 and DXS390 will enable the isolation of additional conserved sequences that will help in understanding both the lesions underlying the pathogenesis of IP1 and the size and extent of the man-mouse homologous block defined by DXF34.…”

Sepsid even-skipped enhancers are functionally conserved in Drosophila despite lack of sequence conservation. by Emily E Hare, Brant K Peterson, Venky N Iyer, Rudolf Meier, Michael B Eisen

“…In exploring this diverse collection of sequences to identify the shared features that account for their similar functions, we found a small number of short (20-30 bp) sequences nearly perfectly conserved among the species. These highly conserved sequences are strongly enriched for pairs of overlapping or adjacent binding sites. …”
Get full text

Formation of a unique cluster of G-quadruplex structures in the HIV-1 Nef coding region: implications for antiviral activity. by Rosalba Perrone, Matteo Nadai, Jerrod A Poe, Ilaria Frasson, Manlio Palumbo, Giorgio Palù, Thomas E Smithgall, Sara N Richter

“…A comprehensive computational analysis of the nef coding region of available strains showed the presence of three conserved sequences that were uniquely clustered. Biophysical testing proved that G-quadruplex conformations were efficiently stabilized or induced by G-quadruplex ligands in all three sequences. …”
Get full text

Importance of Viral Late Domains in Budding and Release of Enveloped RNA Viruses by Lisa Welker, Jean-Christophe Paillart, Serena Bernacchi

“…Late assembly (L) domains are conserved sequences that are necessary for the late steps of viral replication, acting like cellular adaptors to engage the ESCRT membrane fission machinery that promote virion release. …”
Get full text

Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. by Patricia P Coltri, Carla C Oliveira

“…Splicing of primary transcripts is an essential process for the control of gene expression. Specific conserved sequences in premature transcripts are important to recruit the spliceosome machinery. …”
Get full text

Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery by Keigo Fujiwara, Naoko Tsuji, Mayu Yoshida, Hiraku Takada, Shinobu Chiba

“…Several of the identified peptides contain distinct conserved sequences near the C-termini, but are still able to efficiently stall bacterial ribosomes in vitro and in vivo. …”
Get full text

piRNN: deep learning algorithm for piRNA prediction by Kai Wang, Joshua Hoeksema, Chun Liang

“…Identifying piRNAs from small RNA data is a challenging task due to the lack of conserved sequences and structural features of piRNAs. Many programs have been developed to identify piRNA from small RNA data. …”
Get full text

Constructing a DNA ladder Range for Lambda Phage by multiplex PCR by R Gopalakrishnan, S Joseph, S Sellappa

“…Different DNA ladders are available for expected DNA length. Conserved sequences were selected for design of primers to generate DNA fragments of known specific size. …”
Get full text

Evolution and selection in yeast promoters: analyzing the combined effect of diverse transcription factor binding sites. by Daniela Raijman, Ron Shamir, Amos Tanay

“…While such studies enabled the detection of conserved sequences in large genomes, the evolutionary dynamics of regulatory regions as a whole remain poorly understood. …”
Get full text

In silico guided structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: A functional marker based approach. by Geleta Dugassa Barka, Eveline Teixeira Caixeta, Sávio Siqueira Ferreira, Laércio Zambolim

“…Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. …”
Get full text

Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes by Tonissen Kathryn F, Osborne Simone A

“…The mouse and human promoters share some conserved sequences.</p>…”
Get full text

Characterisation of a sexual stage-specific gene encoding ORC1 homologue in the human malaria parasite Plasmodium falciparum. by Li, J, Cox, L

“…PfORC1 contains all conserved sequences in the ORC1/Cdc6/Cdc18 family and displays the highest homology to the Schizosaccharomyces pombe ORC1. …”

A candidate gene for mild mental handicap at the FRAXE fragile site. by Chakrabarti, L, Knight, S, Flannery, A, Davies, K

“…Through the use of conserved sequences adjacent to the FRAXE GCC repeat, we have isolated a 1495 bp cDNA which begins 331 bp distal to the FRAXE site and extends to a region &gt; 170 kb distal in Xq28. …”