Neurons as a model system for cryo-electron tomography by Benoît Zuber, Vladan Lučić

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Advances in cryo-electron tomography and subtomogram averaging and classification by Zhang, P

“…Cryo-electron tomography (cryoET) can provide 3D reconstructions, or tomograms, of pleomorphic objects such as organelles or cells in their close-to-native states. …”

Algorithmic and infrastructural software development for cryo electron tomography by Frosio, T

Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography by Miriam Sarah Weber, Matthias Wojtynek, Ohad Medalia

Subjects: “…cryo-electron tomography…”
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Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography by Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Martin Beck, Hugo Germain

“…Although well-established protocols for isolating protoplasts from different plant tissues are available, they have never been used for studying plant cells using cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET). Here we describe a workflow to prepare root protoplasts from Arabidopsis thaliana plants for cryo-ET. …”
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Cryo electron tomography of native HIV-1 budding sites. by Carlson, L, de Marco, A, Oberwinkler, H, Habermann, A, Briggs, J, Kräusslich, H, Grünewald, K, Gruenewald, K

“…Here, we established a system for studying HIV-1 virus-like particle assembly and release by cryo electron tomography of intact human cells. The lattice of the structural Gag protein in budding sites was indistinguishable from that of the released immature virion, suggesting that its organization is determined at the assembly site without major subsequent rearrangements. …”

Capturing actin assemblies in cells using in situ cryo-electron tomography by Jonathan Schneider, Marion Jasnin

Subjects: “…Cryo-electron tomography workflows…”
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Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography by Eric H.-L. Chen, Chun-Hsiung Wang, Yi-Ting Liao, Feng-Yueh Chan, Yui Kanaoka, Takayuki Uchihashi, Koichi Kato, Longsheng Lai, Yi-Wei Chang, Meng-Chiao Ho, Rita P.-Y. Chen

“…In this study, we use cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM) to directly visualize the pepD2M-induced disruption of the outer and inner membranes of the Gram-negative bacterium Escherichia coli, and compared with a well-known pore-forming peptide, melittin. …”
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Recent advances in infectious disease research using cryo-electron tomography by Daniel Asarnow, Vada A. Becker, Daija Bobe, Charlie Dubbledam, Jake D. Johnston, Jake D. Johnston, Mykhailo Kopylov, Nathalie R. Lavoie, Qiuye Li, Jacob M. Mattingly, Joshua H. Mendez, Mohammadreza Paraan, Jack Turner, Viraj Upadhye, Richard M. Walsh, Meghna Gupta, Edward T. Eng

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The Mechanical Microenvironment Regulates Axon Diameters Visualized by Cryo-Electron Tomography by Di Ma, Binbin Deng, Chao Sun, David W. McComb, Chen Gu

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Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography by Benjamin D Engel, Miroslava Schaffer, Luis Kuhn Cuellar, Elizabeth Villa, Jürgen M Plitzko, Wolfgang Baumeister

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CryoFIB milling large tissue samples for cryo-electron tomography by Sihan Wang, Heng Zhou, Wei Chen, Yifeng Jiang, Xuzhen Yan, Hong You, Xueming Li

“…Abstract Cryo-electron tomography (cryoET) is a powerful tool for exploring the molecular structure of large organisms. …”
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Fully automated, sequential focused ion beam milling for cryo-electron tomography by Tobias Zachs, Andreas Schertel, João Medeiros, Gregor L Weiss, Jannik Hugener, Joao Matos, Martin Pilhofer

“…Cryo-electron tomography (cryoET) has become a powerful technique at the interface of structural biology and cell biology, due to its unique ability for imaging cells in their native state and determining structures of macromolecular complexes in their cellular context. …”
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Correction: Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography by Benjamin D Engel, Miroslava Schaffer, Luis Kuhn Cuellar, Elizabeth Villa, Jürgen M Plitzko, Wolfgang Baumeister

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Cryo-Electron Tomography of <em>Candida glabrata</em> Plasma Membrane Proteins by Cristina Jiménez-Ortigosa, Jennifer Jiang, Muyuan Chen, Xuyuan Kuang, Kelley R. Healey, Paul Castellano, Nikpreet Boparai, Steven J. Ludtke, David S. Perlin, Wei Dai

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Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei by Höög, J, Bouchet-Marquis, C, McIntosh, JR, Hoenger, A, Gull, K

“…In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. …”

Three-dimensional structure of herpes simplex virus from cryo-electron tomography. by Grünewald, K, Desai, P, Winkler, D, Heymann, J, Belnap, D, Baumeister, W, Steven, A

emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging by Himes, B, Zhang, P

“…Such complexes can be studied by cryo-electron tomography (cryo-ET) combined with subtomogram alignment and classification, which in exceptional cases achieves subnanometer resolution, yielding insight into structure-function relationships. …”

Differentiation and characterization of excitatory and inhibitory synapses by cryo-electron tomography and correlative microscopy by Tao, C, Liu, Y, Sun, R, Zhang, B, Qi, L, Shivakoti, S, Tian, C, Zhang, P, Lau, P, Zhou, Z, Bi, G

“…We have developed an integrative approach combining cryo-electron tomography and correlative fluorescence microscopy to visualize 3D ultrastructural features of intact excitatory and inhibitory synapses in their native state. …”

Streamlined structure determination by cryo-electron tomography and subtomogram averaging using TomoBEAR by Nikita Balyschew, Artsemi Yushkevich, Vasilii Mikirtumov, Ricardo M. Sanchez, Thiemo Sprink, Mikhail Kudryashev

“…Abstract Structures of macromolecules in their native state provide unique unambiguous insights into their functions. Cryo-electron tomography combined with subtomogram averaging demonstrated the power to solve such structures in situ at resolutions in the range of 3 Angstrom for some macromolecules. …”
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