Cryo-EM reveals an unprecedented binding site for NaV1.7 inhibitors enabling rational design of potent hybrid inhibitors by Marc Kschonsak, Christine C Jao, Christopher P Arthur, Alexis L Rohou, Philippe Bergeron, Daniel F Ortwine, Steven J McKerrall, David H Hackos, Lunbin Deng, Jun Chen, Tianbo Li, Peter S Dragovich, Matthew Volgraf, Matthew R Wright, Jian Payandeh, Claudio Ciferri, John C Tellis

“…To advance inhibitor design targeting the NaV1.7 channel, we pursued high-resolution ligand-bound NaV1.7-VSD4 structures using cryogenic electron microscopy (cryo-EM). Here, we report that GDC-0310 engages the NaV1.7-VSD4 through an unexpected binding mode orthogonal to the arylsulfonamide inhibitor class binding pose, which identifies a previously unknown ligand binding site in NaV channels. …”
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Fabrication of Yttrium Ferrite Nanoparticles by Solution Combustion Synthesis by A. A. Saukhimov, M. A. Hobosyan, G. C. Dannangoda, N. N. Zhumabekova, S. E. Kumekov, K. S. Martirosyan

“…The structural and magnetic properties of yttrium ferrite before and after annealing at temperature of 1000 °C were investigated by X-ray diffractometry, Differential Scanning Calorimetry (DSC) and cryogenic magnetometry (PPMS, Quantum Design). X-ray diffraction showed that, a broad diffraction peak was found for all samples indicating the amorphous nature of the product. …”
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Innate immunity changes in soccer players after whole-body cryotherapy by Valentina Selleri, Marco Mattioli, Domenico Lo Tartaro, Annamaria Paolini, Giada Zanini, Anna De Gaetano, Roberta D’Alisera, Laura Roli, Alessandra Melegari, Pasqualino Maietta, Ferdinando Tripi, Emanuele Guerra, Johanna Chester, Gustavo Savino, Tommaso Trenti, Andrea Cossarizza, Anna Vittoria Mattioli, Marcello Pinti, Milena Nasi

“…Abstract Whole-body cryotherapy (WBC) consists of short exposure (up to 2–3 min) to dry air at cryogenic temperatures (up to -190 °C) and has recently been applied for muscle recovery after injury to reduce the inflammation process. …”
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Continuous measurements of isotopic composition of water vapour on the East Antarctic Plateau by M. Casado, M. Casado, A. Landais, V. Masson-Delmotte, C. Genthon, C. Genthon, E. Kerstel, E. Kerstel, S. Kassi, L. Arnaud, L. Arnaud, G. Picard, G. Picard, F. Prie, O. Cattani, H.-C. Steen-Larsen, E. Vignon, E. Vignon, P. Cermak

“…Comparison of the results from infrared spectroscopy with laboratory measurements of discrete samples trapped using cryogenic sampling validates the relevance of the method to measure isotopic composition in dry conditions. …”
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Determining spontaneous fission properties by direct mass measurements with the FRS Ion Catcher by Mardor Israel, Dickel Timo, Amanbayev Daler, Ayet San Andrés Samuel, Beck Sönke, Benyamin David, Bergmann Julian, Constantin Paul, Cléroux Cuillerier Alexandre, Geissel Hans, Gröff Lizzy, Hornung Christine, Kripko-Koncz Gabriella, Mollaebrahimi Ali, Miskun Ivan, Plaß Wolfgang R., Pomp Stephan, Rotaru Adrian, Scheidenberger Christoph, Stanic Goran, Will Christian

“…With this method, fission fragments are produced by spontaneous fission from a source that is mounted in a cryogenic stopping cell (CSC), thermalized and stopped within it, and then extracted and transported to a multiple-reflection time-of-flight mass-spectrometer (MR-TOF-MS). …”
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Abstract P-4: Robust Method for Background Subtraction in Serial X-ray Diffraction Data by Egor Marin, Daniil Vakhrameev, Anastasiia Gusach, Aleksandra Luginina, Kirill Kovalev, Wei Liu, Uwe Weierstall, Jaehun Park, Ki Hyun Nam, Cho Yunje, Alexey Mishin, Vadim Cherezov, Valentin Borshchevskiy

“…Their structural studies have been enabled by multiple technological breakthroughs in their heterologous expression, stabilization, crystallization, and crystallographic data collection as well as in cryogenic electron microscopy (cryoEM). During the last decade, serial femtosecond crystallography (SFX) using X-ray free electron lasers (XFELs) has enabled structure determination of previously inaccessible proteins, including several G-protein-coupled receptors (GPCR), that produce only micrometer-sized crystals, thus paving the way towards understanding their activation mechanism and rational drug discovery. …”
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Synthesis of a Chiral 3,6T22-Zn-MOF with a T-Shaped Bifunctional Pyrazole-Isophthalate Ligand Following the Principles of the Supramolecular Building Layer Approach by Dennis Woschko, Simon Millan, Muhammed-Ali Ceyran, Robert Oestreich, Christoph Janiak

“…The small trigonal cross-section of about 6 × 4 Å (considering the van der Waals surface) prevents the access of N₂ and Ar under cryogenic conditions. After activation, only smaller H₂ (at 87 K) and CO₂ (at 195 K) are allowed for gas uptakes of 2 mmol g^–1 and 5.4 mmol g^–1, respectively, in the ultramicroporous material, for which a BET surface area of 496 m²·g^–1 was calculated from CO₂ adsorption. …”
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Production of highly oxygenated organic molecules (HOMs) from trace contaminants during isoprene oxidation by A.-K. Bernhammer, A.-K. Bernhammer, L. Fischer, B. Mentler, M. Heinritzi, M. Simon, A. Hansel, A. Hansel

“…This was successfully achieved by cryogenically trapping lower-volatility compounds such as monoterpenes before isoprene was introduced into the CLOUD chamber.…”
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Developing the intra-firm technology transfer system at the industrial enterprise based on matrix approach by Olga Maslak, Natalia Grishko, Kateryna Vorobiova, Olha Hlazunova, Maria Maslak

“…They include active development which stipulates the extensive use of endogenous factors in terms of favorable environment; moderate growth which is appropriate given certain internal and external problems of enterprises innovative development in general and technology transfer systems in particular; anti-crisis development which is appropriate either in terms of favorable environment given low internal capacity of technology development and transfer or in a difficult situation at macro-level given high transfer potential; “cryogenic” period when the entity is suffering a crisis and there are a lot of external risks, it is reasonable to resort to the “deliberate damping”, being forced to suspend the innovative activity, creation and development of technology transfer system. …”
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Whole-body Cryostimulation: A Rehabilitation Booster by Paolo Capodaglio, Riccardo Cremascoli, Paolo Piterà, Jacopo Maria Fontana

“…WBC is not only a symptomatic physical therapy but rather represents an “adaptation therapy” because of the repeated shock-like cryogenic cold stimulus over the entire body surface that induces reactions in the autonomic, endocrine, circulatory, neuromuscular and immunological systems, resulting in an adaptation that contributes to the restoration of the homeostatic state. …”
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Universal material trends in extraordinary magnetoresistive devices by Ricci Erlandsen, Thierry Désiré Pomar, Lior Kornblum, Nini Pryds, Rasmus Bjørk, Dennis V Christensen

“…By further simulating several high-mobility materials at room and cryogenic temperatures, we conclude that encapsulated graphene and InSb are amongst the most promising candidates for EMR devices showing high magnetoresistance exceeding 10 ^7 % below 1 T at room temperature. …”
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The Potential of Caffeic Acid Lipid Nanoparticulate Systems for Skin Application: In Vitro Assays to Assess Delivery and Antioxidant Effect by Supandeep Singh Hallan, Maddalena Sguizzato, Markus Drechsler, Paolo Mariani, Leda Montesi, Rita Cortesi, Sebastian Björklund, Tautgirdas Ruzgas, Elisabetta Esposito

“…Particularly, morphology has been investigated by cryogenic transmission electron microscopy and small angle X-ray scattering, while mean diameters have been evaluated by photon correlation spectroscopy. …”
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On the Specifics of Formation of the Qualitative Characteristics of Waters and Sediments in the Lena River Delta by A.A. Chetverova, I.V. Fedorova, L.A. Frolova, G.R. Nigamatzyanova, T.V. Skorospekhova, A.A. Shadrina

“…The interpretation of these factors reflects the influence of river catchment area, cryogenic processes in the river delta, and biogeochemical processes. …”
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Design of a Fiber Alkali Vapor Cell for Atomic Magnetometer for Magnetoencephalography Applications by Xuejing Liu, Yanhui Hu, Yang Li, Xudong Wu, Min Chang, Xuedian Zhang

“…Spin exchange relaxation free (SERF) atomic magnetometer (AM), based on the Larmor precession of alkali atoms, is considered a promising candidate for magnetoencephalography (MEG) systems with the advantages of high sensitivity and no need for cryogenic devices. The footprint of the sensor header contains alkali vapor cell and bulk optical elements determining the spatial resolution of the MEG system. …”
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Effect of Calcination Temperature and Chemical Composition of PAN-Derived Carbon Microfibers on N₂, CO₂, and CH₄ Adsorption by Reyna Ojeda-López, Guadalupe Ramos-Sánchez, Cinthia García-Mendoza, Diana C. S. Azevedo, Ariel Guzmán-Vargas, Carlos Felipe

“…Nonetheless, a higher concentration of heteroatoms also causes: (i) a reduction in the adsorption capacity of CO₂ and CH₄ and (ii) open hysteresis loops in N₂ adsorption at cryogenic temperatures. Therefore, the calcination of PAN derived microfibers at temperatures above 800 °C is recommended, which results in materials with suitable micropore volume and a low content of surface heteroatoms, leading to high CO₂ uptake while keeping acceptable selectivity with regards to CH₄ and moderate adsorption enthalpies.…”
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Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition. [version 4; peer review: 2 ap... by David Joseph, Nagana Gowda G. A., Guruprasad Kalthur, Gitanjali Asampille, Vani Lakshmi R., Satish Kumar Adiga, Keyur Raval, Shubhashree Uppangala, Aswathi Cheredath

“…Droplets were subjected to the sensitivity-enhanced nuclear magnetic resonance (NMR)-based profiling using 800 MHz NMR equipped with a cryogenically cooled micro-coil (1.7mm) probe. NMR profile of the embryo culture medium between the two groups were comprehensively assessed. …”
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Magnetoconductivity and Terahertz Response of a HgCdTe Epitaxial Layer by Dmitriy Yavorskiy, Krzysztof Karpierz, Michał Baj, Małgorzata M. Bąk, Nikolai N. Mikhailov, Sergey A. Dvoretsky, Vladimir I. Gavrilenko, Wojciech Knap, Frederic Teppe, Jerzy Łusakowski

“…An epitaxial layer of HgCdTe—a THz detector—was studied in magnetotransmission, magnetoconductivity and magnetophotoconductivity experiments at cryogenic temperatures. In the optical measurements, monochromatic excitation with photon frequency ranging from 0.05 THz to 2.5 THz was used. …”
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Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location by Liao, Shu-Yu, Lee, Myungwoon, Wang, Tuo, Sergeyev, Ivan V., Hong, Mei

“…This motivated us to carry out a systematic evaluation of sample preparation protocols for optimizing the sensitivity of DNP NMR spectra of membrane-bound peptides and proteins at cryogenic temperatures of ~110 K. We show that mixing the radical with the membrane by direct titration instead of centrifugation gives a significant boost to DNP enhancement. …”
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A concept for seeing-limited near-IR spectroscopy on the Giant Magellan Telescope by Furesz, Gabor, Egan, Mark, Hellickson, Timothy H, Malonis, Andrew C., Simcoe, Robert A.

“…Catalog gratings and H2RG detectors are used to minimize cost, and only two cryogenic rotary mechanisms are employed, reducing mechanical complexity. …”
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Science with the XEUS High Time Resolution Spectrometer by Homan, Jeroen, Zdziarski, A. A., Wilms, J., Poutanen, J., Paltani, Stephane, Mereghetti, S., Miller, M. Coleman, Miller, J. M., Mendez, M., Gogus, E., Gilfanov, M., Bhattacharyya, S., Belloni, Tomaso M., Barret, Didier

“…The High Time Resolution Spectrometer (HTRS) is one of the five focal plane instruments, which comprises also a wide field imager, a hard X-ray imager, a cryogenic spectrometer, and a polarimeter. The HTRS is unique in its ability to cope with extremely high count rates (up to 2 Mcts/s), while providing sub-millisecond time resolution and good (CCD like) energy resolution. …”
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