Exploring Novel Variants of the Cytochrome P450 Reductase Gene (<i>POR</i>) from the Genome Aggregation Database by Integrating Bioinformatic Tools and Functional Assays by Maria Natalia Rojas Velazquez, Søren Therkelsen, Amit V. Pandey

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Heterologous expression in E. coli and functional characterization of the tomato CPR enzymes by Won Choi, Seo Young Park, Hyun Min Kim, Thanh Dat Mai, Ju Hui Do, Hye Min Jang, Hyeon Bae Hwang, Eun Gyeong Song, Jae Sung Shim, Young Hee Joung

Subjects: “…NADPH-cytochrome P450 reductase…”
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Co-Expression of a Homologous Cytochrome P450 Reductase Is Required for In Vivo Validation of the <i>Tetranychus urticae</i> CYP392A16-Based Abamectin Resistance in <i>Drosophila</i> by Maria Riga, Aris Ilias, John Vontas, Vassilis Douris

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Identification of Hydroxylation Enzymes and the Metabolic Analysis of Dihydromyricetin Synthesis in <i>Ampelopsis grossedentata</i> by Shuai Zhang, Song Gao, Yu Chen, Sha Xu, Shiqin Yu, Jingwen Zhou

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PHARMACOGENOMICS OF HUMAN P450 OXIDOREDUCTASE by Amit V Pandey, Amit V Pandey, Patrick eSproll

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Interaction Modes of Microsomal Cytochrome P450s with Its Reductase and the Role of Substrate Binding by Francisco Esteves, Philippe Urban, José Rueff, Gilles Truan, Michel Kranendonk

Subjects: “…NADPH-cytochrome P450 reductase…”
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Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories by Pradeepraj Durairaj, Shengying Li

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Liver cytochrome P450-hydroxylation system of tumor-bearing rats under the influence of ?-3 polyunsaturated fatty acids and vitamin D(3) by I. O. Shymanskyi, O. V. Ketsa, M. M. Marchenko, М. М. Veliky

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Exploring <i>Cryptococcus neoformans</i> CYP51 and Its Cognate Reductase as a Drug Target by Yasmeen N. Ruma, Mikhail V. Keniya, Brian C. Monk

“…Type 2 binding of azoles to CnCYP51-6×His and assay of NADPH cytochrome P450 reductase activity confirmed that the heterologously expressed CnCYP51 and CnCPR are functional. …”
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Evaluation of the Protective Effects of Quercetin, Rutin, Naringenin, Resveratrol and Trolox Against Idarubicin-Induced DNA Damage by Haydar Çelik, Emel Arinç

“…The studies in our laboratory have clearly demonstrated that idarubicin can undergo reductive bioactivation by NADPH-cytochrome P450 reductase to free radicals with resulting formation of DNA strand breaks, which can potentially contribute to its genotoxic effects [Çelik, H., Arinç, E., Bioreduction of idarubicin and formation of ROS responsible for DNA cleavage by NADPH-cytochrome P450 reductase and its potential role in the antitumor effect. …”
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The oxidoreductases POR and CYB5R1 catalyze lipid peroxidation to execute ferroptosis by Youwei Ai, Bo Yan, Xiaodong Wang

“…Our recent study showed that two oxidoreductases – NADPH-cytochrome P450 reductase (POR) and NADH-cytochrome b5 reductase (CYB5R1) – transfer electrons to oxygen to generate hydrogen peroxide (H2O2). …”
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Single- and Two-Electron Reduction of Nitroaromatic Compounds by Flavoenzymes: Mechanisms and Implications for Cytotoxicity by Narimantas Čėnas, Aušra Nemeikaitė-Čėnienė, Lidija Kosychova

“…This review addresses the following topics: (i) the intrinsic redox properties of ArNO₂, in particular, the energetics of their single- and two-electron reduction in aqueous medium; (ii) the mechanisms and structure-activity relationships of reduction in ArNO₂ by flavoenzymes of different groups, dehydrogenases-electrontransferases (NADPH:cytochrome P-450 reductase, ferredoxin:NADP(H) oxidoreductase and their analogs), mammalian NAD(P)H:quinone oxidoreductase, bacterial nitroreductases, and disulfide reductases of different origin (glutathione, trypanothione, and thioredoxin reductases, lipoamide dehydrogenase), and (iii) the relationships between the enzymatic reactivity of compounds and their activity in mammalian cells, bacteria, and parasites.…”
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Heme degrading protein HemS is involved in oxidative stress response of Bartonella henselae. by MaFeng Liu, Henri-Jean Boulouis, Francis Biville

“…Purified HemS from B. henselae binds heme and can degrade it in the presence of a suitable electron donor, ascorbate or NADPH-cytochrome P450 reductase. Knocking down the expression of HemS in B. henselae reduces its ability to face H₂O₂ induced oxidative stress.…”
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The catalytic function of cytochrome P450 is entwined with its membrane-bound nature [version 1; referees: 4 approved] by Carlo Barnaba, Katherine Gentry, Nirupama Sumangala, Ayyalusamy Ramamoorthy

“…P450 and its reducing counterparts—cytochrome P450-reductase and cytochrome b5—are membrane-bound proteins located in the cytosolic side of the endoplasmic reticulum. …”
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Network Pharmacology-based Research on Lipid Lowering Mechanism of Walnut Cold-press Meal by GUO Yan, MA Ya-ge, ZHANG Xi, CHEN Chao-yin, ZHAO Sheng-lan

“…This paper explores the action mechanism of walnut cold-press meal in reducing fat from the perspective of network pharmacology.Active components and potential targets of walnut cold-press meal were obtained through TCMSP database and literature mining.GeneCards and other databases were used to predict and screen Hyperlipidemia (HLP) related genes,while Cytoscape 3.7.2 software was applied to construct a “drug-active component-disease-target” network to the formation of walnuts to prevent HLP components and target interactions,and analysis of GO function and KEGG pathway enrichment.Results show that 14 active components of Walnut cold-press meal were identified,among which 7 components have 59 HLP-related targets.HAS2,NCOA2,ESR1,CAT,AR,VEGFA and POR may be the key targets of HLP,with the main signaling pathways being HIF-1 signaling pathway,TNF signaling pathway,Chagas disease,and malaria signaling pathway.HLP is prevented by multi-component,multi-target and multi-pathway,among which the most targeted components are quercetin,rutin,catechin,erucic acid and ellagic acid.Targets most affected by the compounds are hyaluronate synthase 2,nuclear receptor coactivator factor 2,estrogen receptor,catalase,androgen receptor,vascular endothelial growth factor A and NHLPPH--cytochrome P450 reductase.…”
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Redox Proteomic Profile of Tirapazamine-Resistant Murine Hepatoma Cells by Aušra Nemeikaitė-Čėnienė, Per Haberkant, Dalius Kučiauskas, Frank Stein, Narimantas Čėnas

“…On the other hand, the expression of NADPH:cytochrome P450 reductase was responsible for the single-electron reduction in TPZ and for the 2.1-fold decrease. …”
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Systematic Engineering of <i>Saccharomyces cerevisiae</i> for the De Novo Biosynthesis of Genistein and Glycosylation Derivatives by Yongtong Wang, Zhiqiang Xiao, Siqi Zhang, Xinjia Tan, Yifei Zhao, Juan Liu, Ning Jiang, Yang Shan

“…Different combinations of isoflavone synthase, cytochrome P450 reductase, and 2-hydroxyisoflavone dehydratase were tested, followed by pathway multicopy integration, to stably de novo synthesize genistein. …”
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Roles of Ferredoxin-NADP⁺ Oxidoreductase and Flavodoxin in NAD(P)H-Dependent Electron Transfer Systems by Takashi Iyanagi

“…This complex evolves into the covalent FNR (FAD)-Fld (FMN) pair, which participates in a wide variety of NAD(P)H-dependent metabolic pathways as an electron donor, including bacterial sulfite reductase, cytochrome P450 BM3, plant or mammalian cytochrome P450 reductase and nitric oxide synthase isoforms. …”
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Engineering Saccharomyces cerevisiae for the production of dihydroquercetin from naringenin by Shiqin Yu, Mingjia Li, Song Gao, Jingwen Zhou

“…The flavonoid 3′-hydroxylase (F3′H) and cytochrome P450 reductase from different species were co-expressed in S. cerevisiae, and the best strain expressing the P450-reductase enzyme complex (SmF3′H/ScCPR) yielded 435.7 ± 7.6 mg/L of ERI from NAR in the deepwell microplate. …”
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Identification of Novel Antifungal Skeleton of Hydroxyethyl Naphthalimides with Synergistic Potential for Chemical and Dynamic Treatments by Pengli Zhang, Vijai Kumar Reddy Tangadanchu, Chenghe Zhou

“…Biochemical interactions displayed that <b>7f</b> could form a supramolecular complex with DNA to block DNA replication, and constitute a biosupermolecule with cytochrome P450 reductase (CPR) from <i>Candida tropicalis</i> to hinder CPR biological function. …”
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